Effects of cadmium stress on juvenile hormone and molting hormone titers in Spodoptera exigua

doi:10.6048/j.issn.1001-4330.2023.09.025

Abstract

Abstract:

【Objective】 Effects of cadmium stress on juvenile hormone and molting hormone titers in Spodoptera exigua. The effect of heavy metal on growth and development of insects from the perspective of insect physiology in the hope of providing a basis for ecological risk assessment and pollution control of heavy metals.【Methods】 In this study, artificial diet containing four concentrations of Cd²⁺ (0, 0.2,3.2, 51.2 mg/kg) were used to feed the newly hatched larvae of Spodoptera exigua until pupation.Then the titers of juvenile hormone and molting hormone at different growth stages (3rd instar, 5th instar, female adult and male adult) of S.exigua were determined.【Results】 The results of this experiment showed that the titers of juvenile hormone and molting hormone in larvae increased under low concentration cadmium stress (0.2 mg/kg).With the increase of cadmium concentration, the titers of juvenile hormone and molting hormone gradually decreased.The titers of juvenile-preserving hormone and molting hormone increased in Spodoptera exigua under cadmium stress, and the titers of juvenile-preserving hormone and molting hormone did not change significantly with the increase of cadmium concentration.【Conclusion】 The results indicate that cadmium stress has significant effects on the titers of two hormones in S.exigua.

Key words: cadmium; Spodoptera exigua; juvenile hormone; molting hormone

摘要：

【目的】研究重金属镉胁迫对昆虫生长发育的影响,为重金属镉胁迫评价提供依据。【方法】采用镉含量分别为0、0.2、3.2和51.2 mg/kg的人工饲料饲喂甜菜夜蛾初孵幼虫直至化蛹,测定不同虫态(3龄幼虫、5龄幼虫、雌/雄成虫)甜菜夜蛾体内保幼激素和蜕皮激素的滴度。【结果】低浓度镉(0.2 mg/kg)胁迫下甜菜夜蛾幼虫体内保幼激素和蜕皮激素滴度升高;随着镉浓度的升高,保幼激素和蜕皮激素的滴度逐渐下降。镉胁迫下甜菜夜蛾成虫体内保幼激素滴度升高,蜕皮激素滴度降低;随着镉浓度的升高,保幼激素(JH)和蜕皮激素(MH)的滴度无明显变化。【结论】JH和MH是昆虫生长发育和繁殖的重要调控因子,重金属镉胁迫通过改变昆虫体内JH和MH的滴度进而对昆虫生长发育和生殖产生影响。

关键词: 金属镉, 甜菜夜蛾, 保幼激素, 蜕皮激素

CLC Number:

S435.663

WU Menglu, SUN Qingming, WU Jiaojiao, WANG Kai, YANG Yang, YANG Yizhong, SU Honghua. Effects of cadmium stress on juvenile hormone and molting hormone titers in Spodoptera exigua[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2291-2297.

吴梦露, 孙清明, 吴娇娇, 王凯, 杨旸, 杨益众, 苏宏华. 重金属镉胁迫对甜菜夜蛾体内保幼激素和蜕皮激素滴度的影响[J]. 新疆农业科学, 2023, 60(9): 2291-2297.

Figures/Tables 2

References 30

[1]	白建林. 镉对褐飞虱(Nilaparvata lugens Stal)生殖的影响[D]. 福州: 福建农林大学, 2010.
	BAI Jianlin. Effect of Cadmium on Reproduction of Brown Planthopper, Nilaparvata lugens Stal[D]. Fuzhou: Fujian Agriculture and Forestry University, 2010.
[2]	莫少锋, 汤井田, 李康慧, 等. 镉对去卵巢大鼠肾脏的类雌激素作用[J]. 环境与健康杂志, 2015, 32(2): 105-108.
	MO Shaofeng, TANG Jingtian, LI Kanghui, et al. Estrogen-mimicking effects of cadmium on kidneys of ovariectomized rats[J]. Journal of Environment and Health, 2015, 32(2): 105-108.
[3]	贾海梅. 镉对大鼠卵巢孕激素合成的影响及其机理研究[D]. 福州: 福建医科大学, 2007.
	JIA Haimei. Effect and Mechanism of Cadmium on the Progesterone Synthesis of Ovaries[D]. Fuzhou: Fujian Medical University, 2007.
[4]	赵小凡. 昆虫蜕皮激素信号转导途径研究进展[J]. 生命科学, 2010, 22(12): 1208-1214.
	ZHAO Xiaofan. Progress in insect molting hormone signaling transduction pathways[J]. Chinese Bulletin of Life Sciences, 2010, 22(12): 1208-1214.
[5]	毛益文. 褐飞虱Hairy基因的克隆及其功能研究[D]. 杭州: 中国计量大学, 2019.
	MAO Yiwen. Molecular cloning and functional study of Hairy(Hry)from Nilaparvata lugens (Stal)[D]. Hangzhou: China Jiliang University, 2019.
[6]	张长禹. UV胁迫下棉铃虫生殖补偿研究及Hsps基因的克隆与表达[D]. 武汉: 华中农业大学, 2010.
	ZHANG Changyu. Study on the reproductive compensation of Helicoverpa Armigera under uv stress and cloning and expression of hsps genes[D]. Wuhan: Huazhong Agricultural University, 2010.
[7]	蒋丰泽, 郑灵燕, 郭技星, 等. 温度对昆虫繁殖力的影响及其生理生化机制[J]. 环境昆虫学报, 2015, 37(3): 653-663.
	JIANG Fengze, ZHENG Lingyan, GUO Jixing, et al. Effects of temperature stress on insect fertility and its physiological and biochemical mechanisms[J]. Journal of Environmental Entomology, 2015, 37(3): 653-663.
[8]	钱明惠, 任顺祥, 胡琼波. 双斑恩蚜小蜂寄生对烟粉虱体内保幼激素和20-羟基蜕皮酮含量的影响[J]. 昆虫学报, 2006, 49(4): 568-573.
	QIAN Minghui, REN Shunxiang, HU Qiongbo. Effects of endoparasitism byEncarsia bimaculata on the titers of juvenile hormones and 20-hydroxyecdysone in Bemisia tabaci nymphs[J]. Acta Entomologica Sinica, 2006, 49(4): 568-573.
[9]	陈振, 李忠榜, 张绍勇, 等. 喜树碱对小菜蛾体内保幼激素及蜕皮激素滴度的影响[J]. 植物保护学报, 2015, 42(4): 658-662.
	CHEN Zhen, LI Zhongbang, ZHANG Shaoyong, et al. Effects of camptothecin on juvenile hormone and ecdysone titer in diamondback moth Plutella xyllostella[J]. Journal of Plant Protection, 2015, 42(4): 658-662.
[10]	江幸福, 罗礼智, 胡毅. 幼虫食物对甜菜夜蛾生长发育、繁殖及飞行的影响[J]. 昆虫学报, 1999, 42(3): 270-276.
	JIANG Xingfu, LUO Lizhi, HU Yi. Influence of Larval Diets on Development, Fecundity and Flight Capacity of the Beet Armyworm, Spodoptera Exigua[J]. Acta Entomologica Sinica, 1999, 42(3): 270-276.
[11]	余慧灵, 向兴, 袁贵鑫, 等. 溴氰虫酰胺亚致死剂量对甜菜夜蛾生长发育及体内解毒酶活性的影响[J]. 昆虫学报, 2015, 58(6): 634-641.
	YU Huiling, XIANG Xing, YUAN Guixin, et al. Effects of sublethal doses of cyantraniliprole on the growth and development and the activities of detoxifying enzymes in Spodoptera exigua (Lepidoptera: Noctuidae)[J]. Acta Entomologica Sinica, 2015, 58(6): 634-641.
[12]	王学贵, 余慧灵, 梁沛, 等. 氯虫苯甲酰胺诱导甜菜夜蛾细胞色素P450基因上调表达[J]. 昆虫学报, 2015, 58(3): 281-287.
	WANG Xuegui, YU Huiling, LIANG Pei, et al. Chlorantraniliprole induces up-regulated expression of cytochrome P450 genes in Spodoptera exigua (Lepidoptera: Noctuidae)[J]. Acta Entomologica Sinica, 2015, 58(3): 281-287.
[13]	李腾武, 杨崇珍, 何将绪, 等. 甜菜夜蛾人工饲料及饲养技术研究[J]. 昆虫知识, 2001, 38(5): 383-386.
	LI Tengwu, YANG Chongzhen, HE Jiangxu, et al. The artificial diet and the mass rearing method of Spodoptera exigua[J]. Acta Entomologica Sinica, 2001, 38(5): 383-386.
[14]	Hendrickx F, Maelfait JP, Langenbick F. Absence of cadmium excretion and high assimilation result in cadmium biomagnifications in a wolf spider[J]. Ecotox Environ Safe, 2003, 55(3): 287-292. PMID
[15]	Eraly D, Hendrickx F, Backeljau T, et al. Direct and indirect effects of metal stress on physiology and life history variation in field populations of a lycosid spider[J]. Ecotox Environ Safe, 2011, 74(6): 1489-1497. DOI PMID
[16]	吴国星, 高熹, 叶恭银, 等. 取食重金属铜对棕尾别麻蝇亲代及子代生长发育与繁殖的影响[J]. 昆虫学报, 2007, 50(10): 1042-1048.
	WU Guoxing, GAO Xi, YE Gongyin, et al. Effects of dietary copper on the growth, development and reproduction of Boettcherisca peregrine (Diptera: Sarcophagidae)in the parental generation and first filial generation[J]. Acta Entomologica Sinica, 2007, 50(10): 1042-1048.
[17]	曹红妹. 镉/镍对亚洲玉米螟生长发育及生殖行为的影响[D]. 南昌: 江西农业大学, 2015.
	CAO Hongmei. Effects of heavy mental Cd²⁺ and Ni²⁺ on the growth and reproductive behaviors of Ostrinia furnacalls[D]. Nanchang: Jiangxi Agricultural University, 2015.
[18]	胡蒙蒙. Pb²⁺和Cd²⁺在甜菜夜蛾体内的积累与排泄及对种群增长的影响[D]. 扬州: 扬州大学, 2013.
	HU Mengmeng. Accumulation and Excretion Activities of Lead and Cadmium in Spodoptera exigua (Hubner)and the Impact on the Population Increase[D]. Yangzhou: Yangzhou University, 2013.
[19]	Dong S Z, Ye G Z, Zhu J Y, et al. Vitellin of Pteromalus puparum (Hymenoptera: Pteromalidae), a pupal endoparasitoid of Pieris rapae (Lepidoptera: Pieridae): Biochemical characterization, temporal patterns of production and degradation[J]. Journal of Insect Physiology, 2007, 53(5): 468-477. DOI URL
[20]	戈林泉, 吴进才. 昆虫卵黄蛋白及其激素调控的研究进展[J]. 昆虫知识, 2010, 47(2): 236-246.
	GE Linquan, WU Jincai. Research progress in insect vitellin and its hormone regulation[J]. Chinese Bulletin of Entomology, 2010, 47(2): 236-246.
[21]	Cervera A, Maymó A C, Martínez-Pardo R, et al. Vitellogenesis inhibition in Oncopeltus fasciatus females (Heteroptera: Lygaeidae) exposed to cadmium[J]. Journal of Insect Physiology, 2005, 51(8):895-911. PMID
[22]	Adams T S. The role of juvenile hormone in housefly ovarian folliclemorphogenesis[J]. Journal of Insect Physiology, 1974, 20(2): 263-276. PMID
[23]	Caroci A S, Li Y, Noriega F G. Reduced juvenile hormone synthesis in mosquitoes with low teneral reserves reduces ovarian previtellogenic development in Aedes aegypti[J]. Journal of Experimental Biology, 2004, 207(15): 2685-2690. DOI URL
[24]	Song J S, Wu Z X, Wang Z M, et al. Krüppel-homolog 1 mediates juvenile hormone action to promote vitellogenesis and oocyte maturation in the migratory locust[J]. Insect Biochemistry & Molecular Biology, 2014, 52(1): 94-101.
[25]	周娇, 李娟, 翁强, 等. 蜕皮激素对昆虫生长及生殖过程的调控[J]. 应用昆虫学报, 2013, 50(5): 1413-1418.
	ZHOU Jiao, LI Juan, WENG Qiang, et al. The regulation of ecdysteroid on insect growth and productive processes[J]. Chinese Journal of Applied Entomology, 2013, 50(5): 1413-1418.
[26]	杨欣. 蜕皮激素滴度对家蚕幼虫蜕皮进程的调控机制研究[D]. 重庆: 重庆大学, 2020.
	YANG Xin. Regulation Mechanism of larval molting process by the fluctuation of ecdysteroid titer in the domestic silkworm, Bombyx mori[D]. Chongqing: Chongqing University, 2020.
[27]	庞海玉. 20-羟基蜕皮甾酮和川牛膝甾酮对异色瓢虫生物活性及其作用机理研究[D]. 哈尔滨: 东北林业大学, 2013.
	PANG Haiyu. Study on the Biological Activity ang Functional Mechanism of 20-Hydroxyecdysone and Cyasterone on Harmonia axyridis Pallas (Coleoptera, Coccinellidae)[D]. Harbin: Northeast Forestry University, 2013.
[28]	陈英. 保幼激素对棉铃虫成虫生殖、寿命及子代生长发育的影响[D]. 武汉: 华中农业大学, 2013.
	CHEN Ying. Effects of Juvenile Hormone on Reproduction and Longevity in Helicoverpa Armigera, and on the Development of Its F₁ Generation[D]. Wuhan: Huazhong Agricultural University, 2013.
[29]	Pinto L Z, Bitondim M, Simões Z L. Inhibition of vitellogenin synthesis inApis mellifera workers by a juvenile hormone analogue, pyriproxyfen[J]. Journal of Insect Physiology, 2000, 46(2): 153-160. PMID
[30]	Satoshi H, Elizabeth K, Sai M, et al. Effects of the juvenile hormone mimic NC-184 on the development of the reproductive organs and mating behaviour of nymphs of the desert locust, Schistocerca gregaria (Orthoptera: Acrididae)[J]. European Journal of Entomology, 2019, 116: 477-485. DOI URL

镉浓度 Cadmium concentration (mg/kg)	保幼激素滴度 Juvenile hormone titer(ng/g)
镉浓度 Cadmium concentration (mg/kg)	3龄 3rd instar	5龄5th instar	雌成虫 Female adult	雄成虫 Male adult
0	14.75±0.52^b	13.10±0.56^a	5.41±0.76^b	3.73±0.73^b
0.2	17.46±1.28^a	14.75±0.24^a	17.08±2.91^a	20.39±0.27^a
3.2	13.69±0.51^b	10.63±0.52^b	16.53±2.54^a	20.25±0.62^a
51.2	11.63±2.25^b	9.87±0.56^b	18.90±0.38^a	20.63±0.22^a

镉浓度 Cadmium concentration (mg/kg)	保幼激素滴度 Juvenile hormone titer(ng/g)
镉浓度 Cadmium concentration (mg/kg)	3龄 3rd instar	5龄5th instar	雌成虫 Female adult	雄成虫 Male adult
0	14.75±0.52^b	13.10±0.56^a	5.41±0.76^b	3.73±0.73^b
0.2	17.46±1.28^a	14.75±0.24^a	17.08±2.91^a	20.39±0.27^a
3.2	13.69±0.51^b	10.63±0.52^b	16.53±2.54^a	20.25±0.62^a
51.2	11.63±2.25^b	9.87±0.56^b	18.90±0.38^a	20.63±0.22^a

镉浓度 Cadmium concentration (mg/kg)	蜕皮激素滴度(ng/g)Molting hormone titer
镉浓度 Cadmium concentration (mg/kg)	3龄3rd instar	5龄5th instar	雌成虫 Female adult	雄成虫 Male adult
0	17.45±0.86^b	12.58±4.88^b	40.26±0.49^b	32.29±2.33^b
0.2	21.41±1.71^b	30.24±2.00^a	32.56±1.12^a	24.64±1.59^ab
3.2	15.63±2.27^ab	17.81±2.47^ab	31.25±0.78^a	22.86±0.80^a
51.2	9.05±1.00^a	15.20±2.65^b	33.69±2.12^a	32.81±2.19^b

镉浓度 Cadmium concentration (mg/kg)	蜕皮激素滴度(ng/g)Molting hormone titer
镉浓度 Cadmium concentration (mg/kg)	3龄3rd instar	5龄5th instar	雌成虫 Female adult	雄成虫 Male adult
0	17.45±0.86^b	12.58±4.88^b	40.26±0.49^b	32.29±2.33^b
0.2	21.41±1.71^b	30.24±2.00^a	32.56±1.12^a	24.64±1.59^ab
3.2	15.63±2.27^ab	17.81±2.47^ab	31.25±0.78^a	22.86±0.80^a
51.2	9.05±1.00^a	15.20±2.65^b	33.69±2.12^a	32.81±2.19^b