Study on Pollen Germination Characteristics of Jujube and its Response to Gibberellin

doi:10.6048/j.issn.1001-4330.2020.02.009

Xinjiang Agricultural Sciences ›› 2020, Vol. 57 ›› Issue (2): 283-291.DOI: 10.6048/j.issn.1001-4330.2020.02.009

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Study on Pollen Germination Characteristics of Jujube and its Response to Gibberellin

Abudoukayoumu Ayimaiti¹, FAN Dingyu², HAO Qing², ZENG Bin¹, ZHANG Xiangfei¹, YU Zhenfan¹, TANG Yilian¹

1. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China;
2. Institute of Horticulture Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091, China

Received:2019-10-15 Published:2020-03-02
Correspondence author: ZENG Bin (1970-), male, Hubei, associate professor, doctor, master's supervisor, research direction is fruit tree germplasm resources and fruit tree cultivation physiology, (E-mail) zbxnd@163.com
HAO Qing (1969-), male, Aksu, Xinjiang, researcher, master's supervisor, research direction is Xinjiang characteristic fruit tree cultivation, (E-mail) haoqingxj@sohu.com
Supported by:
Supported by the Ear-marked Fund for Synergia of Quality and Efficiency of Forestry and Fruit Industry of Xinjiang Uygur Autonomous Region “Study on the Effect of Gibberellin on the Yield, Quality and Fruit Safety of Jujube” (2019-4202012);Key Subject Project of Horticulture of Xinjiang Uygur Autonomous Region “Exploitation and Utilization of Xinjiang Featured Fruit Germplasm Resources and Breeding and Promotion of Fine Varieties” (2016-10758-3);Central Finance Forestry Reform and Development Program Project "Demonstration and Promotion of Comprehensive Technologies for Quality Improvement and Efficiency Increase in Eco-healthy Jujube Gardens" ([2018] TG08)

赤霉素对枣花繁殖生物学特性的影响

阿布都卡尤木·阿依麦提¹, 樊丁宇², 郝庆², 曾斌¹, 张翔飞¹, 余镇藩¹, 唐义莲¹

1. 新疆农业大学林学与园艺学院, 乌鲁木齐 830052;
2. 新疆农业科学院园艺作物研究所,乌鲁木齐 830091

通讯作者: 曾斌(1970-),男,湖北人,副教授,博士,硕士生导师,研究方向为果树种质资源及果树栽培生理,(E-mail)zbxnd@163.com
郝庆(1969-),男,新疆阿克苏人,研究员,硕士生导师,研究方向为新疆特色果树栽培,(E-mail)haoqingxj@sohu.com
作者简介:阿布都卡尤木·阿依麦提(1995-),男,新疆喀什人,硕士研究生,研究方向为果树栽培生理,(E-mail)315894588@qq.com
基金资助:
自治区林业专项资金林果业提质增效科技专项资金“赤霉素对红枣产量质量及果品安全的影响研究”(2019-4202012);新疆维吾尔自治区园艺学重点学科项目“新疆特色果树种质资源开发利用与优良品种选育推广”(2016-10758-3);中央财政林业改革发展资金项目“生态健康枣园提质增效综合技术示范与推广”([2018]TG08)

Abstract

Abstract: 【Objective】 Spraying gibberellin in flowering stage is an important measure to promote fruit setting of jujube trees. To have a clearer understanding of its effect on the Biological characteristics of jujube flower reproductionwill provide a theoretical basis for the scientific application of gibberellin in production. 【Method】 The effects of gibberellin (GA₃) on the germination and vitality of jujube pollen were analyzed by using the in vitro culture medium, iodine potassium iodide staining and benzidine hydrogen peroxide method. 【Result】 By spraying different concentrations of gibberellin during the flowering stage of jujube, the pollen germination rate, pollen viability and pollen amount of Huizao and Junzao were significantly different from those of the control. Low concentration (10-30 mg/L) gibberellin significantly promoted pollen germination rate, pollen tube length, pollen viability, pollen quantity and stigma receptivity of the fruits. With the increase of gibberellin concentrations, the germination characteristics of pollen were inhibited; the pollen germination rate and pollen viability of 7 d after gibberellin treatment were not significantly different from those of the control. 【Conclusion】 The response of pollen germination, pollen tube length, pollen quantity and pollen activity to exogenous gibberellin was promoted by low concentration and inhibited by high concentration.

Key words: jujube; gibberellin;pollen germination rate; pollen vitality; stigma receptivity

摘要： 【目的】 研究赤霉素对枣花繁殖生物学特性的影响,为生产上赤霉素的科学应用提供理论依据。【方法】 以灰枣和骏枣的鲜花粉为材料,于盛花期喷施赤霉素(GA₃)处理,采用离体培养基,I-KI(碘-碘化钾)染色法,联苯胺-过氧化氢法测定,分析赤霉素对红枣花粉萌发和活力特性的影响。【结果】 低浓度(10~30 mg/L)赤霉素对灰枣和骏枣花粉萌发率、花粉管长度、花粉活力、花粉量和柱头可授性有较显著的促进作用。随着赤霉素浓度的升高,对花粉的萌发特性产生抑制作用;赤霉素处理后7 d的花粉萌发率和花粉活力跟对照没有显著差异。【结论】 枣花粉萌发、花粉管长度、花粉量和花粉活力特性,对外源赤霉素的响应表现为低浓度促进,高浓度抑制作用。

关键词: 枣, 赤霉素, 花粉萌发率, 花粉活力, 柱头可授性

CLC Number:

S665

Abudoukayoumu Ayimaiti, FAN Dingyu, HAO Qing, ZENG Bin, ZHANG Xiangfei, YU Zhenfan, TANG Yilian. Study on Pollen Germination Characteristics of Jujube and its Response to Gibberellin[J]. Xinjiang Agricultural Sciences, 2020, 57(2): 283-291.

阿布都卡尤木·阿依麦提, 樊丁宇, 郝庆, 曾斌, 张翔飞, 余镇藩, 唐义莲. 赤霉素对枣花繁殖生物学特性的影响[J]. 新疆农业科学, 2020, 57(2): 283-291.

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Study on Pollen Germination Characteristics of Jujube and its Response to Gibberellin

赤霉素对枣花繁殖生物学特性的影响

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