Comparative Analysis of Amino Acid Sequence of Gastrin and Cholecystokinin and Their Receptors Based on Informatics

doi:10.6048/j.issn.1001-4330.2019.05.019

Xinjiang Agricultural Sciences ›› 2019, Vol. 56 ›› Issue (5): 955-963.DOI: 10.6048/j.issn.1001-4330.2019.05.019

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Comparative Analysis of Amino Acid Sequence of Gastrin and Cholecystokinin and Their Receptors Based on Informatics

YANG Yang, HOU Yu, ZHAO Shu-lin, LI Lu-lu,SUN Xiao-yan, Aili Adilai, ZHAO Hong-qiong

College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China

Received:2019-03-21 Published:2019-05-20
Correspondence author: ZHAO Hong-qiong ( 1974 - ), female, native place: Xichong, Sichuan, Associate professor; research field: The structure and function of biologically active substances,zhaohongqiong@sina.com
Supported by:
Supported by the National Natural Science Foundation of China (31560684) and The Graduate Research Innovation Project of Xinjiang Agricultural University (XJAUGRI2016005)

胃泌素和胆囊收缩素及其受体氨基酸序列信息学比较分析

杨阳, 侯宇, 赵树林, 李璐璐, 孙小燕, 阿迪莱·艾力, 赵红琼

新疆农业大学动物医学学院,乌鲁木齐 830052

通讯作者: 赵红琼(1974-),女,四川西充人,博士,研究方向为生物活性物质结构与功能,(E-mail)zhaohongqiong@sina.com
作者简介:杨阳(1992-),女,新疆人,硕士研究生,研究方向为生物活性物质结构与功能,(E-mail)565029651@qq.com
基金资助:
国家自然科学基金(31560684);新疆农业大学研究生科研创新(XJAUGRI2016005)

Abstract

Abstract: 【Objective】 Gastrin (GAS) and cholecystokinin (CCK) are found to have more similarities in their physiological functions, but these two hormones have different affinities to their receptors (CCK-A and CCK-B). The present study aims to compare the amino acid sequences of sheep GAS and CCK and their receptors with those of other animals by bioinformatics.【Method】The UniProt database was used to search the amino acid sequences of various animal GAS, CCK and their receptors and these sequences were compared by Phylogenetic Tree Analysis. 【Result】The comparative results showed that seven amino acids at C-terminal of GAS were identical in all animals except for chicken and fish. Ten amino acids at C-terminal of CCK were identical in all animals except for guinea pigs. Amino acid sequence of GAS-34 and CCK-33 were identical in sheep and cattle. Phylogenetic analysis showed that CCK-A and CCK-B were on the same branch in sheep, cattle, rats, mice, dogs and cats. Full length amino acid sequences of GAS, CCK and their receptors (including signal peptide and propeptide) revealed that sequences of avian and fish are largely different from mammals. Whereas, amino acid sequence similarity between sheep CCK-A and CCK-B was only 45.094%.【Conclusion】The above analysis demonstrated that the C-terminal amino acid sequences of GAS and CCK were highly conserved among different animals, whereas the N-terminus was a variable region. These bioinformatics analyses can be used as reference for the studies of antigen design for these two polypeptides and their receptor protein, and the selection of active polypeptide in different animals

Key words: gastrin; CCK; CCK-A; CCK-B; amino acid sequence

摘要： 【目的】胃泌素(GAS)和胆囊收缩素(CCK)具有较多相似性,但两种激素与其受体(CCK-A和CCK-B)亲和力不同,研究利用生物信息学的方法,比对羊GAS和CCK及其受体氨基酸序列与其它动物序列的不同,为不同动物这两种多肽及其受体蛋白抗原设计和活性多肽筛选等提供参考。【方法】通过UniProt数据库检索各种动物GAS和CCK及其受体氨基酸序列进行比对,并进行全氨基酸序列进化树分析。【结果】除了鸡和鱼,其他动物GAS的C-端7个氨基酸完全相同;除了豚鼠外,CCK的C-端10个氨基酸序列完全相同;羊与牛的GAS-34及CCK-33氨基酸序列完全相同。羊与牛,大鼠与小鼠、狗与猫的CCK-A和CCK-B均分别在同一个分支上;从GAS和CCK及受体全氨基酸序列(包括信号肽和原肽)来看,禽类和鱼类序列同哺乳动物差距均较大;绵羊CCK-A与CCK-B氨基酸序列相似性仅为45.094%。【结论】GAS和CCK的C-端氨基酸序列在不同动物之间均具有高度的保守性,而N-端为变化区域。

关键词: 胃泌素, 胆囊收缩素, CCK-A, CCK-B, 氨基酸序列

CLC Number:

S813

YANG Yang, HOU Yu, ZHAO Shu-lin, LI Lu-lu,SUN Xiao-yan, Aili Adilai, ZHAO Hong-qiong. Comparative Analysis of Amino Acid Sequence of Gastrin and Cholecystokinin and Their Receptors Based on Informatics[J]. Xinjiang Agricultural Sciences, 2019, 56(5): 955-963.

杨阳, 侯宇, 赵树林, 李璐璐, 孙小燕, 阿迪莱·艾力, 赵红琼. 胃泌素和胆囊收缩素及其受体氨基酸序列信息学比较分析[J]. 新疆农业科学, 2019, 56(5): 955-963.

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Comparative Analysis of Amino Acid Sequence of Gastrin and Cholecystokinin and Their Receptors Based on Informatics

胃泌素和胆囊收缩素及其受体氨基酸序列信息学比较分析

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