不同因素影响下棉田土壤中大丽轮枝菌微菌核的数量特征

doi:10.6048/j.issn.1001-4330.2021.03.015

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (3): 522-531.DOI: 10.6048/j.issn.1001-4330.2021.03.015

• 园艺特产·植物保护·畜牧兽医·微生物·农业生态环境 • 上一篇下一篇

不同因素影响下棉田土壤中大丽轮枝菌微菌核的数量特征

刘海洋¹, 王伟¹, 张仁福¹, 温切木·阿布列孜², 姚举¹

1.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091;
2.阿勒泰市菜篮子工程办公室, 新疆阿勒泰836500

收稿日期:2020-09-05 出版日期:2021-03-20 发布日期:2021-03-30
通信作者: 姚举(1969-),男,山东人,研究员,研究方向为棉花病虫害综合防控,(E-mail)yaoju500@sohu.com
作者简介:刘海洋(1982-),男,山东人,副研究员,博士,研究方向为棉花病害综合防控,(E-mail)liuhaiyang001@163.com
基金资助:
新疆维吾尔自治区科技支疆项目计划(2019E0244);新疆农业科学院青年科技骨干创新能力培养项目(xjnkq-2020015);自治区公益性科研院所基本科研业务费(KY201807);国家重点研发计划(2017YFD0201106,2017YFD0201903)

Quantitative Characteristics of Microsclerotia of Verticillium dahliae in Cotton Field Soil under Different Factors

LIU Haiyang¹, WANG Wei¹, ZHANG Renfu¹, Wenqiemu Abulizi², YAO Ju¹

1. Key Laboratory of Integrated Crop Pest Management in Northwestern Oasis of China,MOARA, Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;
2. The Shopping Basket Program Office of Altay, Altay Xinjiang 836500, China

Received:2020-09-05 Online:2021-03-20 Published:2021-03-30
Correspondence author: YAO Ju (1969- ), male, Shandong, Professor, research field: study on integrated prevention and control of cotton diseases and pests,(E-mail)yaoju500@sohu.com
Supported by:
the Science and Technology Supporting Xinjiang Project of Xinjiang Autonomous Region (2019E0244), Project of Renovation Capacity Building for the Young Sci-Tech Talents Sponsored by Xinjiang Academy of Agricultural Sciences (xjnkq-2020015); Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region (KY201807); the National Key R&D Program of China (2017YFD0201903 and 2017YFD0201106)

摘要/Abstract

摘要： 【目的】 土壤中大丽轮枝菌的微菌核是棉花黄萎病发生的关键因子,研究外界因素对土壤中微菌核数量的影响特征,为棉花黄萎病的防治提供参考意义。【方法】 利用选择性分离培养与荧光定量PCR技术,检测采自新疆棉花黄萎病田内不同处理组土壤中的微菌核,并结合室内盆栽试验进行验证。【结果】 大丽轮枝菌微菌核在棉田土壤中呈聚集分布,不同抗病性棉花品种根围微菌核的数量无明显差异。花铃期时'新陆中66号根围微菌核的数量显著高于新稻11号,至吐絮期时新稻11号根围微菌核数量显著增加,与新陆中66号根围相比已无显著差异。耐病品种中植棉2号以及中植棉2号＋新稻11号混种处理土壤中微菌核数量与感病品种军棉1号及军棉1号+新稻11号混种处理均无显著差异。水淹20~30 d时土壤中微菌核的数量显著上升,至60 d土壤中微菌核数量略下降,至150 d时微菌核数量升至最高,是初期微菌核数量的5倍;种稻处理土壤中微菌核的消长动态与对照一致,至150 d时微菌核数量是初期的8倍。【结论】 大丽轮枝菌微菌核在土壤中聚集分布,其数量与棉花品种的抗病性无明显相关,大田种植旱稻前期能延缓微菌核的增长。盆栽水淹能够显著促进土壤中微菌核数量的增长,种稻未能抑制甚至促进了土壤中微菌核数量的增长。

关键词: 大丽轮枝菌; 微菌核; 棉花黄萎病; 选择性分离; 根围土壤

Abstract: 【Objective】 Microsclerotia of Verticillium dahliae in soil is the basis of cotton Verticillium wilt. This project aims to clarify the influence of external factors on microsclerotia in soil and provide a solution for the prevention and control of the disease.【Method】 The microsclerotia in soils collected from cotton field with Verticillium wilt under different treatments were detected using selective isolation and culture technology, and verified by indoor pot experiment.【Result】 Microsclerotia were aggregated in the soil of cotton field. Its number in the cotton rhizosphere was not significantly correlated with its disease resistance. Comparing cotton with upland rice, at the flowering and boll-forming stages, the number of microsclerotia in the cotton rhizosphere was significantly higher than that in the rice rhizosphere. At the harvest stage, the number of microsclerotia in the rice rhizosphere increased significantly, but showed no significant difference with the number of microsclerotia in the cotton rhizosphere. Indoor pot experiment revealed no significant differences in the number of microsclerotia in the rhizosphere soil between disease-resistant varieties Zhongzhimian 2, Zhongzhimian 2 + rice treatment and susceptible variety Junmian 1, Junmian 1 + rice mixed treatment. Water flooding was performed for the soil. After 20-30 days of waterflooding, the number of microsclerotia in soil increased significantly, but decreased slightly at the 60th day. After 150 days, the number of microsclerotia increased to the highest level, which was five times as much as that in the initial stage. The dynamics of growth and decline of microsclerotia in soil under rice + waterflooding treatment were consistent with those in blank control, presenting a trend of rapid increase, then decrease and then increase. The number of microsclerotia at the 150th day was 8 times as much as that in the initial stage.【Conclusion】 Microsclerotia are aggregated in the soil of cotton field. The number of microsclerotia in the cotton rhizosphere is not significantly correlated with its disease resistance. Planting upland rice in field can delay the growth of microsclerotia in early stage. Water flooding significantly promotes the growth of microsclerotia in potted planting soil. Rice planting fails to inhibit or even fail to promote the growth of microsclerotia in soil.

Key words: Verticillium dahliae; microsclerotia; Verticillium wilt; selective isolation; rhizosphere soil

中图分类号:

S562
S188

刘海洋, 王伟, 张仁福, 温切木·阿布列孜, 姚举. 不同因素影响下棉田土壤中大丽轮枝菌微菌核的数量特征[J]. 新疆农业科学, 2021, 58(3): 522-531.

LIU Haiyang, WANG Wei, ZHANG Renfu, Wenqiemu Abulizi, YAO Ju. Quantitative Characteristics of Microsclerotia of Verticillium dahliae in Cotton Field Soil under Different Factors[J]. Xinjiang Agricultural Sciences, 2021, 58(3): 522-531.

参考文献

[1] 刘海洋, 王琦, 王伟, 等.新疆棉花黄萎病的发生及其病原菌的分子鉴定与ISSR分析[J].植物保护学报, 2018, 45(6): 1194-1203.
LIU Haiyang, WANG Qi, WANG Wei, et al.Molecular identification and ISSR analysis of Verticillium dahliae and the current status of cotton Verticillium wilt in Xinjiang [J].Journal of Plant Protection, 2018, 45(6):1194-1203.
[2] 刘海洋, 姚举, 张仁福, 等.黄萎病不同发生程度棉田中土壤微生物多样性[J].生态学报, 2018, 38(5): 1619-1629 .
LIU Haiyang, YAO Ju, ZHANG Renfu, et al.Analysis of soil microbial diversity in cotton fields differing in occurrence of cotton Verticillium wilt in Xinjiang [J].Acta Ecologica Sinica, 2018, 38(5): 1619-1629.
[3] 商文静, 陈婷, 白应文, 等.大丽轮枝菌微菌核的萌发条件及致死温度[J].菌物学报, 2013,32(6): 986-992.
SHANG Wenjing, CHEN Ting, BAI Yingwen, et al.Germination condition and lethal temperature for microsclerotia of Verticillium dahlia [J].Mycosystema, 2013, 32(6): 986-992.
[4] 商文静, 魏锋, 冯小军, 等.棉田大丽轮枝菌微菌核的空间分布及其抽样技术[J].西北农业学报, 2014, 23(4): 192-197.
SHANG Wenjing, WEI Feng, FENG Xiaojun, et al. Spatial pattern and sampling method for microsclerotia of Verticillium dahliae in soil of cotton field [J].Acta Agriculturae Boreali-occidentalis Sinica, 2014, 23(4): 192-197.
[5] 杨家荣, 商鸿生, 高立强.土壤环境因素对棉花黄萎病菌微菌核存活的影响[J].植物病理学报, 2004, 34(2): 180-183.
YANG Jiarong, SHANG Hongsheng, GAO Liqiang.The effect of soil habitat factors on survival of microsclerotia of Verticillium dahliae of cotton [J].Acta Phytopathologica Sinica, 2004, 34(2): 180-183.
[6] 薛磊, 徐万里, 顾美英, 等.pH及盐分对大丽轮枝菌微菌核形成的影响[J].西北农业学报, 2017, 26(10): 1520-1528.
XUE Lei, XU Wanli, GU Meiying, et al.Effect of pH and salinity on microsclerotia formation of Verticillium dahliae[J].Acta Agriculturae Boreali-occidentalis Sinica, 2017, 26(10): 1520-1528.
[7] 刘小龙, 马建江, 管吉钊, 等.连作对棉田土壤枯-黄萎病菌数量及细菌群落的影响[J].棉花学报, 2015, 27(1): 62-70.
LIU Xiaolong, MA Jianjiang, GUAN Jizhao, et al. Effects of continuous cropping on numbers of Fusarium oxysporum, Verticillium dahliae and soil bacterial community in cotton field [J].Cotton Science, 2015, 27(1): 62-70.
[8] Inderbitzin P, Ward J, Barbella A, et al.Soil microbiomes associated with Verticillium wilt suppressive broccoli and chitin amendments are enriched with potential biocontrol agents [J].Phytopathology, 2017, 108(1): 31-43.
[9] 刘海洋, 王兰, 努尔孜亚, 等.棉田深翻对棉花黄萎病发病及其微菌核分布影响的初步研究[J].新疆农业科学, 2010, 47(5): 932-935.
LIU Haiyang, WANG Lan, NU Erziya, et al.Effect of deap plough on occurrence of cotton Verticillium wilt and microsclerotia distribution [J].Xinjiang Agricultural Sciences, 2010, 47(5): 932-935.
[10] Lópezescudero F J, Mwanza C, Blancoloópez M A.Reduction of Verticillium dahliae microsclerotia viability in soil by dried plant residues [J].Crop Protection, 2007, 26(2): 127-133.
[11] 姚琴, 王美入, 杨家荣, 等.韭菜等几种植物根系分泌物对棉花黄萎病菌的影响[J].植物保护, 2013, 39(6): 37-42.
YAO Qin, WANG Meiru, YANG Jiarong, et al.Effects of root exudates on the pathogen of cotton Verticillium wilt [J].Plant Protection, 2013, 39(6): 37-42.
[12] Huisman O C, Ashworth L J.Influence of crop rotation on survival of Verticillium albo-atrum in soils [J].Phytopathology, 1976,66(8): 978-981.
[13] Borza T, Beaton B, Govindarajan A, et al.Incidence and abundance of Verticillium dahliae in soil from various agricultural fields in Prince Edward Island, Canada [J].European Journal of Plant Pathology, 2018, 151(3): 825-830.
[14] Bilodeau G J, Koike S T, Uribe P, Martin F N.Development of an assay for rapid detection and quantification of Verticillium dahliae in soil[J].Phytopathology, 2012, 102(3): 331-343.
[15] 冯争光, 鹿秀云, 彭巧慧, 等.一种新的检测土壤棉花黄萎菌微菌核的选择性培养基[J].华北农学报,2004,(19):, 44-47.
FENG Zhengguang, LU Xiuyun, PENG Qiaohui, et al.A novel selective medium for the detection of the microsclerotia [J].Acta Agriculturae-Boreali Siica, 2004,(19): 44-47.
[16] 魏锋, 余真真, 商文静, 等.土壤大丽轮枝菌微菌核的快速定量检测[J].植物病理学报, 2013, 43(5): 449-457.
WEI Feng, YU Zhenzhen, SHANG Wenjing, et al.Rapid detection and quantification of Verticillium dahliae microsclerotia in soil [J].Acta Phytopathologica Sinica, 2013, 43(5): 449-457.
[17] Bilodeau G J, Koike S T, Uribe P, et al.Development of an assay for rapid detection and quantification of Verticillium dahliae in soil [J].Phytopathology, 2012,102(3): 331-343.
[18] Knox O G G, Gupta V V S R, Lardner R.Field evaluation of the effects of cotton variety and GM status on rhizosphere microbial diversity and function in Australian soils[J].Soil Research, 2014, 52(2): 203-215.
[19] 王守正, 王海燕, 李洪连, 等.植物微生物区系和植物抗病性研究[J].河南农业科学, 2001, 30(5): 20-23.
WANG Shouzheng, WANG Haiyang, LI Honglian, et al.Studies on plant microflora and disease resistance of plant [J].Journal of Henan Agricultural Sciences, 2001, 30(5): 20-23.
[20] 吴传德, 延欣芳.棉花抗枯萎病品种连作防病效果的研究[J].植物保护学报, 1985, 12(3): 195-200.
WU Chuande, YAN Xinfang.Study on the effect of continuous cropping of cotton against fusarium wilt [J].Acta Phytophylacica Sinica, 1985, 12(3): 195-200.
[21] 吴玉香, 沈晓佳, 房卫平, 等.陆地棉根系分泌物对黄萎病菌生长发育的影响[J].棉花学报, 2007, 19(4): 286-290.
WU Yuxiang, SHEN Xiaojia, FANG Weiping, et al.The effects of cotton root exudates on growth and development of Verticillium dahliae [J].Cotton Science, 2007, 19(4): 286-290.
[22] Neal J L, Atkinso T G, Larson R I.Change in the rhizosphere microflora of spring wheat induced by disomic substitytion of a chromosome [J]. Microbiology, 1970, 16(3): 153-158.
[23] 王夏雯, 余翔, 乔俊卿, 等.西瓜茬后种植稻麦对土壤微生物数量和西瓜枯萎病发生的影响[J].江苏农业学报, 2015, 31(6): 1291-1295.
WANG Xiawen, YU Xiang, QIAO Junqing, et al.Effect of rice-wheat rotation after watermelon season on the amount of soil microbes and the incidence of Fusarium wilt [J].Acta agriculturae Jiangsu, 2015, 31(6): 1291-1295.
[24] 苏世鸣, 任丽轩, 霍振华, 等.西瓜与旱作水稻间作改善西瓜连作障碍及对土壤微生物区系的影响[J].中国农业科学, 2008, 41(3): 704-712.
SU Shiming, REN Lixuan, HUO Zhenhua, et al.Effects of intercropping watermelon with rain fed rice on Fusarium wilt and the microflora in the rhizosphere soil [J].Scientia Agricultura Sinica, 2008, 41(3): 704-712.
[25] Pavlou G C, Vakalounakis D J. Biological control of root and stem rot of greenhouse cucumber, caused by Fusarium oxysporum f.sp.radicis cucumerinum, by lettuce soil amendment [J].Crop Protection, 2005, 24(2): 135 -140.
[26] Bais H P, Weir T L, Perry L G, et al.The role of root exudates in rhizosphere interactions with plants and other organisms [J].Annual Review of Plant Biology, 2006, 57(1): 233-266.
[27] 马云艳, 顾美英, 徐万里, 等.棉秆生物炭对大丽轮枝菌生长及毒素作用的影响[J].干旱地区农业研究, 2015, 33(5): 217-224.
MA Yunyan, GU Meiying, XU Wanli, et al.Influences of cotton biochar on growth and toxin effect of Verticillium dahliae [J].Agricultural Research in the Arid Areas, 2015, 33(5): 217-224.

不同因素影响下棉田土壤中大丽轮枝菌微菌核的数量特征

Quantitative Characteristics of Microsclerotia of Verticillium dahliae in Cotton Field Soil under Different Factors

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	阳妮, 玛依拉·玉素音, 杨延龙, 李春平, 张大伟, 徐海江, 赖成霞. 黄萎病枯斑型与黄化型病症棉花叶片的植物挥发物对比[J]. 新疆农业科学, 2023, 60(8): 1975-1986.
[2]	王静怡, 佐长赓, 牛新湘, 杨红梅, 楚敏, 王宁, 林青, 王有武, 娄恺, 史应武. 生防菌在棉田土壤中定殖数量与防病作用相关性[J]. 新疆农业科学, 2023, 60(1): 178-184.
[3]	刘海洋, 王伟, 张仁福, 温切木·阿布列孜, 姚举. 利用生防菌防治棉花黄萎病效果的制约因素[J]. 新疆农业科学, 2022, 59(1): 155-161.
[4]	赖成霞, 玛依拉·玉素音, 石必显, 李春平, 姜梦竹, 郑子漂, 杨栋. 助剂激健在防治棉花黄萎病中的效果[J]. 新疆农业科学, 2021, 58(12): 2220-2227.
[5]	石志钰, 裴雅琨, 朱玉涛, 贾玉姣, 胡晓倩, 侯士聪, 侯玉霞. 基于数字图像棉花黄萎病诊断与防治的管理远程监测[J]. 新疆农业科学, 2020, 57(6): 1166-1174.
[6]	许泰百, 萨吉达木·艾则孜, 吴琼, 李玉华, 李进, 郭庆元. 基于PCR检测的4种土传病害病原菌侵染棉花的动态分析[J]. 新疆农业科学, 2019, 56(5): 910-918.
[7]	蔡梦杭,徐灿,刘启,俞燕,黄家风. 北疆棉区棉花黄萎病菌培养性状及致病力分化[J]. 新疆农业科学, 2019, 56(1): 93-103.
[8]	俞燕,蔡梦杭,徐灿,黄家风. 不同碳氮比对棉花黄萎病菌主要生物学性状及致病力的影响[J]. 新疆农业科学, 2019, 56(1): 84-92.
[9]	萨吉达木·艾则孜,赵志强,阿孜古丽·阿不力孜,马依努尔·米吉提,郭庆元. 基于PCR检测棉花4种病原菌在新疆北部分布[J]. 新疆农业科学, 2018, 55(2): 293-303.
[10]	戴翠荣, 练文明, 邰红忠, 卢金宝, 吴博, 王献礼, 贺美球, 武刚, 赵静. 棉花黄萎病发病程度对海岛棉产量和纤维品质的影响[J]. 新疆农业科学, 2017, 54(11): 2022-2027.
[11]	孔德真;华东来;王秀珍;刘步仓;祝建波;王爱英. dGFP-GUS基因标记新疆棉花黄萎病原菌的研究[J]. , 2016, 53(1): 1-8.
[12]	毛建才;张婷;高云;宋雯;高峰. 一种新的大丽轮枝菌基因敲除载体的构建[J]. , 2015, 52(10): 1878-1883.
[13]	刘海洋;王伟;张仁福;武刚;姚举. 新疆棉花黄萎病发生调查及病原菌系统进化分析[J]. , 2015, 52(1): 65-71.
[14]	吴博;李新裕;张玉斌;但红霞;陈小飞;刘婷. 大丽轮枝菌微菌核快速繁殖技术研究[J]. , 2014, 51(8): 1526-1531.
[15]	王丽丽;符桂华;马金贵;日孜旺古丽;郎玉瑶;帕提古丽;李克梅. 乌昌地区马铃薯黄萎病菌分离与鉴定[J]. , 2014, 51(4): 667-672.