新疆红肉苹果果实性状及MYB10启动子基因型鉴定分析

doi:10.6048/j.issn.1001-4330.2020.12.007

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (12): 2213-2220.DOI: 10.6048/j.issn.1001-4330.2020.12.007

• 园艺特产·贮藏保鲜加工·植物保护 • 上一篇下一篇

新疆红肉苹果果实性状及MYB10启动子基因型鉴定分析

闫鹏, 朱燕飞, 梅闯, 冯贝贝, 韩立群, 艾沙江·买买提, 马凯, 王继勋

新疆农业科学院园艺作物研究所/农业部新疆地区果树科学观测试验站,乌鲁木齐 830091

收稿日期:2019-03-13 出版日期:2020-12-20 发布日期:2020-12-24
通信作者: 王继勋(1965-),男,研究员,研究方向为果树资源与设施栽培,(E-mail)ee_wjx@163.com
作者简介:闫鹏(1981-),男,江苏南通人,副研究员,研究方向为果树资源与栽培,(E-mail)xaasyysyp@163.com
基金资助:
新疆维吾尔自治区公益性科研院所基本科研业务费专项(KYGY2016116);新疆维吾尔自治区科技援疆项目(2018E02048);国家现代苹果产业技术体系建设专项(CARS-27)

Analysis on Fruit Characteristics and Genotype of MYB10 Promoter in Xinjiang Red-Fleshed Apple

YAN Peng, ZHU Yanfei, MEI Chuang, FEN Beibei, HAN Liqun, Aishangjian Maimaiti, MA Kai, WANG Jixun

Research Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences /Scientific Observing and Experimental Station of Pomology Xinjiang, Ministry of Agriculture, Urumqi 830091

Received:2019-03-13 Online:2020-12-20 Published:2020-12-24
Correspondence author: WANG Jixun(1965－),male,Researcher,Research orientation: Fruit tree resources and Facility cultivation,(E-mail) ee_wjx@163.com
Supported by:
Basic Scientific R&D Program of Public Welfare Research Institutes of Xinjiang (KYGY2016116);S&T Supporting Xinjiang Project(201802048);China Agriculture Research System (CARS-27)

摘要/Abstract

摘要： 【目的】 分析新疆红肉苹果(Malus sieversii f. Neidzwetzkyana (Dieck) Langenf.)的果实特性及MYB10基因的启动子类型,为研究新疆的红肉基因类型及红肉苹果资源的利用研究奠定基础。【方法】 测定果实单果重、纵径、横径等性状指标并观察果肉颜色,设计引物,利用PCR克隆的方法,进行启动子类型检测。【结果】 果肉颜色多为淡红色,丝状或片状分布,HX-1为血红色果肉全红,单果重变异系数较大,最大单果重为124.57 g,果形指数最大值1.01,最小值0.82;可溶性固形物最大值17.36,最小值10.66;果梗粗最大值2.50 mm,最小值1.02 mm;果梗长度最大值34.36 mm,最小值13.48 mm;MYB10启动子类型全部为R₆R₁型。【结论】 29份资源的果实性状的遗传多样性较为丰富,果肉的呈色各有特点,但MYB10启动子类型全部一致为R6R₁型。

关键词: 红肉苹果; MYB10; 启动子基因型

Abstract: 【Objective】 To study the promoter type of red fresh gene MYB10 in Xinjiang red fresh apple (Malus sieversiif neidzwetzkyana (Dieck) Langenf and the fruit characteristics. The results lay a foundation for further study on red fresh apple breeding in Xinjiang. 【Methods】 The promoter type of 29 red fresh apple were detected by PCR cloning,measured fruit weight,diameter and observed the color of the flesh. 【Results】 The color of flesh was light red, The red part was not continuously distributed in the pulp.The variation coefficients of friut weight were 57.10%,the maximum of single fruit weight was 124.57 g;The shape index of fruit maximum was 1.01, the minimum was 0.82; The soluble solids content maximum was 17.36, minimum value was 10.66; The thickness of stalk maximum was is 2.50 mm, minimum value was 1.02 mm;The length of stalk maximum was 34.36 mm,minimum value was 13.48 mm;The MYB10 promoter type of 29 germplasm red fresh apple was R6R1. 【Conclusion】 The same type of promoter of 29 red fresh apples was R6R1, The fruit characters had abundant genetic diversity and The red part in the pulp was not continuously distributed.

Key words: red fresh apple; MYB10; promoter

中图分类号:

S661.1

闫鹏, 朱燕飞, 梅闯, 冯贝贝, 韩立群, 艾沙江·买买提, 马凯, 王继勋. 新疆红肉苹果果实性状及MYB10启动子基因型鉴定分析[J]. 新疆农业科学, 2020, 57(12): 2213-2220.

YAN Peng, ZHU Yanfei, MEI Chuang, FEN Beibei, HAN Liqun, Aishangjian Maimaiti, MA Kai, WANG Jixun. Analysis on Fruit Characteristics and Genotype of MYB10 Promoter in Xinjiang Red-Fleshed Apple[J]. Xinjiang Agricultural Sciences, 2020, 57(12): 2213-2220.

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新疆红肉苹果果实性状及MYB10启动子基因型鉴定分析

Analysis on Fruit Characteristics and Genotype of MYB10 Promoter in Xinjiang Red-Fleshed Apple

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