盐胁迫对葡萄苗期多胺和激素代谢的影响

doi:10.6048/j.issn.1001-4330.2018.01.008

摘要/Abstract

摘要： 【目的】 研究盐胁迫条件下,葡萄叶片中多胺含量、多胺氧化酶、多胺合成酶活性以及激素的动态变化,研究不同盐胁迫强度对多胺类物质及激素代谢的影响。【方法】 采用盆栽基质培养模拟盐胁迫环境,设置4个NaCl处理梯度:0 (CK)、1、2和3 g/kg。多胺和激素含量的测定采用高效液相色谱法,多胺合成及分解酶活性的测定采用紫外分光光度计比色法。【结果】 盐胁迫处理后,葡萄叶片中多胺含量和脱落酸含量较对照而言显著增加,玉米素含量显著降低,随胁迫时间的延长,Put、Spm、Spd、ABA以及PAO、DAO、ADC、ODC、SAMDC活性均呈先上升后下降趋势,在10 d及10 d后达到峰值。ZT呈逐渐下降的趋势,并随胁迫强度增大而减小;进行显著性分析后,叶片中多胺含量与Put、Spm、Spd含量呈极显著正相关,DAO、ADC、ODC活性与Put含量呈显著正相关,PAO与SAMDC活性与Spm和Spd含量也呈极显著正相关。【结论】 3种多胺经酶的合成代谢后和激素共同缓解葡萄苗所遭受到的盐胁迫,期间主要发生作用的是多胺合成酶,盐胁迫下多胺与激素含量之间的变化关系也十分密切。

关键词: 盐胁迫; 葡萄; 多胺; 激素; PAO; DAO; ADC; ODC; SAMDC

Abstract: 【Objective】 To study the dynamic changes of polyamine content, polyamine oxidase, polyamine synthase activity and hormones in grape leaves under salt stress, and explore the effects of different salt stress intensity on the metabolism of polyamines and hormones.【Method】 The pot culture was used to simulate the salt stress environment, and 4 NaCl treatment gradients were set up: 0 g/kg (CK), 1 g/kg, 2 g/kg, 3 g/kg. Polyamine content and hormone content were determined by high performance liquid chromatography (HPLC). Polyamine synthesis and decomposition enzyme activity were determined by ultraviolet spectrophotometry.【Result】 After salt stress treatment, polyamine content in grape leaves and abscisic acid content significantly increased compared with the control and zeatin content decreased significantly with the prolonging of stress time. Activities of Put, Spm, Spd, ABA, PAO, DAO, ADC, ODC, SAMDC increased first and then declined, reaching the peak in 10 days or after 10 days. ZT gradually decreased, and decreased with the increase of stress intensity. After significant analysis, leaf polyamine content and Put, Spm, Spd content were positively correlated. The activities of DAO, ADC, ODC were significantly correlated with Put content and the activity of SAMDC was positively correlated with the contents of Spm and PAO.【Conclusion】 Metabolism of three polyamines by enzymes and hormones can alleviate salt stress in grape seedling stage, during which the main action is the polyamine synthase. The relationship between the contents of polyamine and hormone under salt stress is also very close.

Key words: salt stress; grape; polyamines; hormones; PAO; DAO; ADC; ODC; SAMDC

中图分类号:

S663.1

孙琳琳,郁松林,赵丰云,杨湘,杨夕. 盐胁迫对葡萄苗期多胺和激素代谢的影响[J]. 新疆农业科学, 2018, 55(1): 66-73.

SUN Lin-lin, YU Song-lin, ZHAO Feng-yun, YANG Xiang, YANG Xi. Effects of Salt Stress on Polyamine and Hormone Metabolism in Grape Seedling Stage[J]. Xinjiang Agricultural Sciences, 2018, 55(1): 66-73.

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