新疆茄子绒菌斑病原菌的分离及鉴定

doi:10.6048/j.issn.1001-4330.2024.02.022

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (2): 455-460.DOI: 10.6048/j.issn.1001-4330.2024.02.022

• 园艺特产·土壤肥料·植物保护 • 上一篇下一篇

新疆茄子绒菌斑病原菌的分离及鉴定

罗文芳(), 周军辉, 何伟(), 许建军()

新疆农业科学院植物保护研究所/农业农村部西北荒漠作物有害生物综合治理重点实验室/新疆农业生物安全重点实验室,乌鲁木齐 830091

收稿日期:2023-06-21 出版日期:2024-02-20 发布日期:2024-03-19
通信作者: 许建军(1972-),男,河南人,研究员,研究方向为蔬菜病虫害防治,(E-mail)xjj72@163.com；何伟(1980-),男,安徽人,副研究员,研究方向为蔬菜病虫害防治技术,(E-mail)181113882@qq.com
作者简介:罗文芳(1990-),女,河南人,助理研究员,研究方向为蔬菜病虫害防治,(E-mail)576263465@qq.com
基金资助:
新疆维吾尔自治区重大科技专项(2022A02005-3);新疆现代农业产业技术体系(XJARS-07)

Isolation and identification of the Passalora nattrassii HR-1 from aubergine in Xinjiang

LUO Wenfang(), Zhou Junhui, HE Wei(), XU Jianjun()

Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis/Ministry of Agriculture and Rural Affairs, Xinjiang Key Laboratory of Agricultural Bio-safety, Institute of Plant Protection/Xinjiang Academy of Agricultural Sciences, Urumqi Xinjiang 830091, China

Received:2023-06-21 Online:2024-02-20 Published:2024-03-19
Correspondence author: XU Jianjun(1972-), male, from Henan, research fellow,research area:control of vegetables diseases and insect pests,(E-mail)xjj72@163.com；HE Wei(1980-), male, from Anhui,research associate, research area: Vegetable diseases and insect pests prevention and control technology,(E-mail)181113882@qq.com
Supported by:
Major R&D Program Project of Xinjiang Uygur Autonomous Region(2022A02005-3);XinJiang Agriculture Research System(XJARS-07)

摘要/Abstract

摘要：

【目的】分离与鉴定设施茄子上新发现的1种病害,为该病害的精准防治提供科学依据。【方法】试验设在新疆巴音郭楞蒙古自治州和硕县采用随机取样法,调查设施茄子上新发现病害的田间发生危害情况,采用组织分离法、柯赫氏法则、形态学观察和分子生物学方法鉴定该病害。【结果】菌株HR-1是该病害的病原菌。菌株HR-1与已报道的灰毛茄钉孢(Passalora nattrassii)同源性高达99.0%以上,菌株HR-1(MW362742.1)与P.nattrassii处于同一分支。【结论】菌株HR-1为灰毛茄钉孢(P.nattrassii),新疆设施茄子叶部新发现的病害为茄子绒菌斑病。

关键词: 茄子; 致病性; 病原菌鉴定; 灰毛茄钉孢

Abstract:

【Objective】 The authors identified one new disease on aubergine in facility eggplant in Heshuo County, Mongolian Autonomous Prefecture of Bayingolin, Xinjiang, and identified the species of the disease, hoping to provide a scientific basis for accurate control of the disease.【Methods】 Random sampling was used to investigate and clarify the damage caused by the disease in the field, and the new disease of aubergine was identified using tissue isolation, Koch's rule, morphological observations and molecular biology to determine the taxonomic status of the pathogen.【Results】 The Koch's Law results indicated that strain HR-1 was the causal agent of the disease.Strain HR-1 was more than 99.0% homologous with the reported Passalora nattrassii.Strain HR-1(MW362742.1)was in the same branch as P.nattrassii.【Conclusion】 The strain HR-1 was identified as P.nattrassii, a newly discovered disease of eggplant foliage in Xinjiang.

Key words: aubergine; pathogenicity; identification of pathogenic bacteria; Passalora nattrassii

中图分类号:

S436.41

罗文芳, 周军辉, 何伟, 许建军. 新疆茄子绒菌斑病原菌的分离及鉴定[J]. 新疆农业科学, 2024, 61(2): 455-460.

LUO Wenfang, Zhou Junhui, HE Wei, XU Jianjun. Isolation and identification of the Passalora nattrassii HR-1 from aubergine in Xinjiang[J]. Xinjiang Agricultural Sciences, 2024, 61(2): 455-460.

图/表 7

表1 茄子叶部病害病斑分级标准

Tab.1 The classification standard for leaf disease and spot of eggplant

级别 Grade	划分标准 Classification standard
0	叶片上无病斑
1	叶片上发现零星病斑
2	病斑面积为叶面积的1 /4
3	病斑面积为叶面积的1 /4~1 /3
4	病斑面积为叶面积的1 /3以上或落叶

图1 茄子绒菌斑病田间发病症状注:A:叶片正面发病症状;B:叶片背面发病症状

Fig.1 Field symptoms on eggplant leaves caused by Passalora nattrassii Note:A:Symptoms on the upside of eggplant leaves;B:Symptoms on the underside of eggplant leaves

表2 茄子绒菌斑病病情

Tab.2 The disease investigation for Passalora nattrassii

编号 Number	调查总叶片数 Investigate the number of leaves	病叶数 Number of disease leaves	发病率 Incidence of a disease (%)	病情指数 Disease index
1号棚 No.1 shed	125	125	100	58.16±4.77
2号棚 No.2 shed	125	125	100	62.40±3.32
3号棚 No.3 shed	125	125	100	60.60±5.09
4号棚 No.4 shed	125	125	100	63.04±2.85
5号棚 No.5 shed	125	125	100	65.13±1.94

图2 柯赫氏法则验证结果注:A:发病叶片;B:空白对照

Fig.2 Koch's rule of validation results Note:A:Diseased leaves;B:control

图3 茄子绒菌斑病病原菌形态注:A:菌落正面;B:菌落背面;C:分生孢子;D:分生孢子梗

Fig.3 The shape of Passalora nattrassii Note:A:Top of the colony;B:Bottom of the colon;C:Conidium;D:Conidiophore

图4 病原菌的ITS序列PCR扩增产物电泳图注:M:DL2000 marker;CK:阴性对照;HR-1:菌株HR-1

Fig.4 Electrophoregrams of PCR amplification products of ITS sequences of the pathogen Note:M:DL2000 marker;CK:negative control;HR-1:strains HR-1

图5 病原真菌基于ITS序列构建的系统发育树

Fig.5 Phylogenetic tree constructed by pathogenic fungi based on ITS sequence

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新疆茄子绒菌斑病原菌的分离及鉴定

Isolation and identification of the Passalora nattrassii HR-1 from aubergine in Xinjiang

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