不同砧木对酿酒葡萄马瑟兰花色苷种类和含量的影响

doi:10.6048/j.issn.1001-4330.2023.06.020

摘要/Abstract

摘要：

【目的】研究不同砧木对酿酒葡萄马瑟兰果皮花色苷种类和含量的影响。【方法】以2018年嫁接的酿酒葡萄马瑟兰为材料,砧木分别为SO4、5BB、3309M和101-14,以未嫁接的为对照,利用LCMS/MS法测定果皮花色苷的种类和含量。【结果】不同砧木嫁接的马瑟兰葡萄果皮中有48种花色苷种类(锦葵苷8种、飞燕草苷12种、矮牵牛苷8种、芍药苷7种、花青苷9种、天竺葵素4种)和11种非花色苷(黄酮5种、黄烷酮2种、原花青素4种)种类。马瑟兰果皮中以锦葵苷、飞燕草苷、矮牵牛苷、芍药苷、花青苷为主,锦葵苷占50%左右,飞燕草苷约占33.00%,并检测到了极少量的天竺葵素。花色苷单体中,锦葵色素-3-O-葡萄糖苷含量最高,约占45%。【结论】砧木SO4下调了总花色苷含量,比对照减低了11.860%,但与对照间不存在极显著差异,砧木5BB、3309M、101-14分别比对照上调了26.621%、2.759%和12.629%,M/3309M、M/101-14花色苷含量与对照间无极显著差异,M/5BB的总花色苷含量与对照间存在极显著差异。

关键词: 马瑟兰; 砧木; 花色苷

Abstract:

【Objective】 To study the effects of different rootstocks on the composition and relative content of anthocyanins in Marselan grape. 【Methods】 In this study, Marserlan, a wine grape grafted in 2018, was used as the test material and the stocks were SO4,5BB, 3309M, and 101-14, and their species and content were determined using the LCMS/MS method with ungrafted ones as a control.【Results】 A total of 48 anthocyanins and 11 nonanthocyanins were indetified in Marselan grape skin, including Malvidin(Mv), Delphinidin(Dp), Petunidin(Pt), Peonidin(Pn), Cyanindin(Cy), Pelargnidin(Pg), nonanthocyanins including flavonoids, flavonol glycoside, proanthocyanidins. The main anthocyanins were Malvidin(Mv), Delphinidin(Dp), Petunidin(Pt), Peonidin(Pn), Cyanindin(Cy), there were less Pelargnidin (Pg), Malvidin(Mv) accounted for about 50%, Delphinidin(Dp) accounted for about 33%. In all of the anthocyanins, Malvidin 3-O-glucoside content was the highest, about 45%.【Conclusion】 The total anthocyanins contents of SO4 is down-regulated and it is 11.860%lower than that of the control. There is no extremely significant difference, while the total anthocyanins contents of 5BB, 3309M and 101-14 are up-regulated, which are 26.621%, 2.759%and 12.629%respectively. There are no extremely significant difference in the anthocyanins contents between M/3309M, M/101-14 and the control, but there is significant difference between M/5BB and the control.

Key words: Marselan; rootstock; anthocyanin

中图分类号:

S663.1

户金鸽, 白世践, 陈光, 蔡军社. 不同砧木对酿酒葡萄马瑟兰花色苷种类和含量的影响[J]. 新疆农业科学, 2023, 60(6): 1466-1475.

HU Jinge, BAI Shijian, CHEN Guang, CAI Junshe. Effects of different rootstocks on the composition and content of anthocyanin in Marselan wine grape[J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1466-1475.

图/表 7

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仪器 Instruments	型号 Instrument model	品牌 Brand
LC-MS/MS	QTRA 6500+	SCIEX
离心机The centrifuge	5424R	Eppendorf
电子天平Electronic balance	AS 60/220.R2	RADWAG
球磨仪Ball mill instrument	MM400	Retsch
多管旋涡振荡器 Multi-tube vortex oscillator	MIX-200	上海净信
超声清洗仪 Ultrasonic cleaning apparatus	KQ5200E	昆山舒美

仪器 Instruments	型号 Instrument model	品牌 Brand
LC-MS/MS	QTRA 6500+	SCIEX
离心机The centrifuge	5424R	Eppendorf
电子天平Electronic balance	AS 60/220.R2	RADWAG
球磨仪Ball mill instrument	MM400	Retsch
多管旋涡振荡器 Multi-tube vortex oscillator	MIX-200	上海净信
超声清洗仪 Ultrasonic cleaning apparatus	KQ5200E	昆山舒美

试剂Reagent	级别Level	品牌Brand
甲醇Methanol	色谱纯	Merck
甲酸Formic acid	色谱纯	Sigma-Aldrich
盐酸 Hydrochloric acid	优级纯	信阳市化学试剂厂
标准品 Standard sample	>99%	isoReag

试剂Reagent	级别Level	品牌Brand
甲醇Methanol	色谱纯	Merck
甲酸Formic acid	色谱纯	Sigma-Aldrich
盐酸 Hydrochloric acid	优级纯	信阳市化学试剂厂
标准品 Standard sample	>99%	isoReag

项目Items	Equation	r
Del	Y=1. 344 1e5X-312.583 59	0.991 92
Del-3,5-O-diglu	Y=1.344 165X-312.583 59	0.991 92
Del-3-sam-5-glu	Y=7.358 06e4X -1 694.587 64	0.99 370
Del-3-O-sop	Y=1.344 165e5X-312.583 59	0.991 92
Del-3-O-gal	Y=4.597 38 e5X-4 695.942 08	0.991 44