新疆农业科学 ›› 2022, Vol. 59 ›› Issue (12): 2924-2932.DOI: 10.6048/j.issn.1001-4330.2022.12.007

• 作物遗传育种·耕作栽培·种质资源 • 上一篇    下一篇

153份新疆冬小麦品种3个条锈病抗性基因的分子检测

路子峰1(), 时晓磊1, 李玉姗1, 崔燕华2, 仙鹤2, 严勇亮1, 张金波1, 高海峰3, 丛花1()   

  1. 1.新疆农业科学院农作物品种资源研究所,乌鲁木齐 830091
    2.新疆农业科学院综合试验场,乌鲁木齐 830012
    3.新疆农业科学院植物保护研究所,乌鲁木齐 830091
  • 收稿日期:2021-12-20 出版日期:2022-12-20 发布日期:2023-01-30
  • 通信作者: 丛花
  • 作者简介:路子峰(1986-),男,河南辉县人,副研究员,研究方向为农作物种质资源创新利用,(E-mail)luzifeng712@126.com
  • 基金资助:
    国家自然科学基金(31660389);新疆农业科学院青年基金(xjnkq-2016002);乌鲁木齐市科学技术计划项目(Z161210002)

Molecular Detection of Three Yr Genes in 153 Winter Wheat Varieties from Xinjiang

LU Zifeng1(), SHI Xiaolei1, LI Yushan1, CUI Yanhua2, XIAN He2, YAN Yongliang1, ZHANG Jinbo1, GAO Haifeng3, CONG Hua1()   

  1. 1. Institute of Crop Germplasm Resources, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
    2. Anningqu Comprehensive Experimental Field, Xinjiang Academy of Agricultural Sciences, Urumqi 830012, China
    3. Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2021-12-20 Online:2022-12-20 Published:2023-01-30
  • Correspondence author: CONG Hua
  • Supported by:
    The National Natural Science Foundation of China(31660389);Xinjiang Academy of Agricultural Sciences for Young Scientists Found(xjnkq-2016002);Urumqi Science and Technology Program Project(Z161210002)

摘要: 【目的】 研究条锈病抗性基因Yr 9、Yr 26、Yr Tp1在新疆153份冬小麦品种中的分布,为新疆小麦抗病育种提供理论依据。【方法】 利用SSR分子标记,采用PCR扩增和聚丙烯酰胺凝胶电泳方法,检测供试材料中是否含有小麦条锈病抗性基因Yr 9、Yr 26和Yr Tp1。【结果】 在123份冬小麦地方品种和30份冬小麦育成品种中,Yr 9基因的检出率分别为78.86%和63.33%,Yr 26基因的检出率分别为95.12%和73.33%,Yr Tp1基因的检出率分别为88.62%和60%。在123份冬小麦地方品种中,有87份供试材料能同时检测出3个条锈病抗病基因,占比70.73%;28份供试材料能同时检测出2个条锈病抗性基因(Yr 9+Yr 26、Yr 9+Yr Tp1、Yr 26+Yr Tp1),占比22.76%;1份供试材料只能检测出Yr 9基因;5份供试材料只能检测出Yr 26基因。在30份冬小麦育成品种中,15份供试材料可以同时检测出3个条锈病抗性基因,占比50%;7份供试材料可以同时检测到2个抗性基因(Yr 9+Yr 26、Yr 26+Yr Tp1)的材料,占比23.33%。【结论】 携带Yr 9、Yr 26、Yr Tp1小麦条锈病抗性基因的新疆冬小麦种质资源丰富,可以作为抗病育种的中间材料。

关键词: 冬小麦品种; 小麦条锈病; 抗病基因; 分子检测

Abstract:

【Objective】 To clarify the distribution of stripe rust resistant genes Yr 9, Yr 26 and Yr Tp1 in 153 winter wheat varieties from Xinjiang with a view to providing theoretical basis for resistance breeding of Xinjiang winter wheat.【Methods】 The SSR marker of stripe rust resistant genes, polymerase chain reaction and polyacrylamide Gel electrophoresis were used to detect whether the tested wheat varieties contain Yr 9, Yr 26 and Yr Tp1 genes.【Results】 The percentage of 123 winter wheat landraces and 30 cultivars from Xinjiang that carried Yr 9 was 78.86% and 63.33%, respectively; Yr 26 was 95.12% and 73.33%, respectively; Yr Tp1 was 88.62% and 60%, respectively; In 123 winter wheat landraces, we identified that all the three resistant genes in 87 varieties (70.73%), two resistant genes—the gene recombination types including Yr 9+Yr 26, Yr 9+Yr Tp1, Yr 26+Yr Tp1- in 28 varieties (22.76%), only Yr 9 in 1 variety, only Yr 26 in 5 varieties; In 30 winter wheat cultivars, we detected all the three resistant genes in 15 varieties (50%), two resistant genes—the gene recombination types including Yr 9+Yr 26andYr 26+Yr Tp1 in 7 varieties (23.33%).【Conclusion】 Above all, it is concluded that the winter wheat germplasm in Xinjiang carrying the Yr genes are abundant and these germplasms can be used as intermediate materials in disease resistant breeding.

Key words: winter wheat; wheat stripe rust; resistance genes; molecular detection

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

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