伊犁河谷小麦条锈病菌源地调查研究

doi:10.6048/j.issn.1001-4330.2017.09.014

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (9): 1679-1687.DOI: 10.6048/j.issn.1001-4330.2017.09.014

伊犁河谷小麦条锈病菌源地调查研究

李伟华¹, 刘太国¹, 高海峰², 刘博¹, 吴伟³, 李宁静⁴, 高利¹, 陈万权¹

1.植物病虫害生物学国家重点实验室/中国农业科学院植物保护研究所,北京 100193;
2.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091;
3.新源县农业技术推广站,新疆新源 835800;
4.新疆维吾尔自治区植物保护站伊犁工作站,新疆伊宁 835000

收稿日期:2017-07-28 发布日期:2020-07-22
通信作者: 刘太国(1974-),男,黑龙江明水人,副研究员,博士,研究方向为麦类作物真菌病害,(E-mail)tgliu@ippcaas.cn;陈万权(1962-),男,四川丰都人,研究员,研究方向为植物病害流行学,(E-mail)wqchen@ippcaas.cn
作者简介:李伟华(1981-),女,博士,研究方向为植物病害流行学,(E-mail)moxingyin@163.com
基金资助:
国家973课题“病原菌毒性变异途径及规律”(2013CB127701);国家自然科学基金项目“小麦条锈菌高致病性新致病类群V26的流行预测”(31371884);自治区科技支疆课题“新疆小麦锈病和白粉病监测及可持续防控技术研究与应用”(2013911092);国家现代农业产业技术体系(CARS-3)

Investigation into Wheat Stripe Rust Pathogen in the Valley of Yili River of Xinjiang, China

LI Wei-hua¹, LIU Tai-guo¹, GAO Hai-feng², LIU Bo¹, WU Wei³, LI Ning-jing⁴, GAO Li¹, CHEN Wan-quan¹

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, West Yuanmingyuan Road 2, Beijing 100193, China;
2. Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences / Key Laboratory of Integrated Pest Management of Crops in Northwestern Oasis, Ministry of Agriculture, P. R..China, Urumqi 830091,China;
3. Agrotechnique Extension Center of Xinyuan County, Xinyuan Xinjiang 835800,China;
4. Yili Plant Protection Station of Xinjiang Uygur Autonomous Region, Yining Xinjiang 835000,China

Received:2017-07-28 Published:2020-07-22
Correspondence author: LIU Tai-guo (1974-), male, native place: Mingshui, Heilongjiang. Associate Professor, research field: Wheat fungal diseases. (E-mail)tgliu@ippcaas.cn; CHEN Wan-quan (1962-), male, native place: Fengdu, Chongqing. Professor, research field: Plant disease epidemiology. (E-mail)wqchen@ippcaas.cn
Supported by:
The Major State Basic Research Development Program of China (973 Program) "The Variation and Pattern of Virulence Genes from Important Pathogenic Fungi of Wheat"(2013CB127701); The National Natural Science Foundation of China "The Epidemiology and Forecasting of New Race V26 Variants of Puccinia striiformis with Highly Virulence Gene"(31371884); Autonomous Region Science and Technology Program "Research and Application of Monitoring and Sustainable Control of Wheat Rust and Powdery Mildew in Xinjiang" (2013911092); National Modern Agricultural Industrial Technology System (CARS-3)

摘要/Abstract

摘要： 【目的】实地调查分析新疆伊犁地区新源县田间小麦条锈病和野外小檗锈病发生情况,为揭示新疆小麦条锈菌的群体遗传多样性以及小檗在小麦条锈病发生流行中的作用提供基础数据。【方法】利用中国小麦条锈病菌鉴别寄主,对2013~2015年新源县小麦条锈菌进行生理小种鉴定,对异果小檗种类鉴定、锈孢子DNA提取及PCR检测。【结果】新源县小麦条锈菌主要类群有Hybrid46类群,出现频率高达31.25%;水源11类群出现频率为22.92%;贵农22类群出现频率为18.75%;中四类群出现频率为6.25%;其他类群出现频率为20.83%。异果小檗锈病鉴定结果表明,异果小檗锈病中存在小麦条锈病,条锈菌锈子器的检出率在4.7%~5.7%。【结论】新源县小麦条锈菌主要为Hybrid46类群、水源11类群、贵农22类群。异果小檗锈病中存在小麦条锈病,条锈菌锈子器的检出率在4.7%~5.7%。

关键词: 小麦条锈病; 异果小檗; 分子检测; 小种

Abstract: 【Objective】 The purpose of this project is to investigate the rust-infected Berberisheteropoda Schneid in spring wheat in order to accumulate data on the genetic diversity and virulence variation of Puccinia striiformis f. sp. tritici (Pst) population in Xinyuan of Xinjiang and the control of wheat stripe rust in China in the future.【Method】The race analysis was carried out with Chinese differentials for Pst from Xinyuan populations in 2013-2015. We also identified the species of B. heteropoda Schneid, extracted the DNA from aeciospores and PCR reactions were amplified.【Result】In Xinyuan, the main pathotypes were Hybrid46 group at a rate of 31.25%, Suwon 11 group at a rate of 22.92%, Guinong 22 group at a rate of 18.75%, Zhong 4 group at a rate of 6.25% and unclassified group at a rate of 20.83%. Aecia on B. heteropoda Schneid were identified at a rate of 4.7%-5.7% by PCR detection with specific molecular marker of Pst.【Conclusion】The pathotypes of Xinyuan populations consisted of Hybrid 46 group, Suwon 11 group, Guinong 22 group and other undesignated races. Aecia of Pst on B. heteropoda Schneid was identified at a rate of 4.7%-5.7% by PCR amplifications with molecular marker specific to Pst.

Key words: wheat stripe rust; B. heteropoda Schneid; molecular detection; race

中图分类号:

S435.121

李伟华, 刘太国, 高海峰, 刘博, 吴伟, 李宁静, 高利, 陈万权. 伊犁河谷小麦条锈病菌源地调查研究[J]. 新疆农业科学, 2017, 54(9): 1679-1687.

LI Wei-hua, LIU Tai-guo, GAO Hai-feng, LIU Bo, WU Wei, LI Ning-jing, GAO Li, CHEN Wan-quan. Investigation into Wheat Stripe Rust Pathogen in the Valley of Yili River of Xinjiang, China[J]. Xinjiang Agricultural Sciences, 2017, 54(9): 1679-1687.

参考文献

[1] Chen, X. M. (2005). Epidemiology and control of stripe rust [puccinia striiformis f. sp. tritici] on wheat. Canadian Journal of Plant Pathology, 27(3):314-337.
[2] 李振岐, 曾士迈. 中国小麦锈病 [M]. 北京: 中国农业出版社, 2002.
   LI Zhen-qi, ZENG Shi-mai.(2002).Wheat rust in China [M].Beijing:China Agriculture Press.(in Chinese)
[3] Chen, W., Wellings, C., Chen, X., Kang, Z., & Liu, T. (2014). Wheat stripe (yellow) rust caused by puccinia striiformis f. sp. tritici. Molecular Plant Pathology, 15(5): 433-446.
[4] 李晶, 曾娟, 姜玉英, 等. 新疆小麦条锈病发生特点及流行规律初探. 中国植保导刊, 2010,30(6): 16-19.
   LI Jing,ZENG Juan,JIANG Yu-ying,et al. (2010).Occurrence characteristics and Regularities of wheat stripe rust in Xinjiang [J].China Plant Protection,36(6):16-19.(in Chinese)
[5] 何海明, 郭文超, 谢震宇. 伊犁河谷冬小麦锈病发生与流行预测模型的研究. 新疆农业科学, 2007, 44(s1): 131-133.
   HE Hai-ming, GUO Wen-chao, XIE Zhen-yu. (2007). Study on the occurrence and epidemic prediction model of winter wheat rust in Yili River valley [J]. Xinjiang Agricultural Sciences, 44 (s1):131-133. (in Chinese)
[6] Yue, J., Lesj, S., & Martin, C. Century-old mystery of puccinia striiformis life history solved with the identification of berberis as an alternate host. Phytopathology, 2010,100(5): 432-435.
[7] Wang, M. N., & Chen, X. M. (2013). First report of oregon grape (mahonia aquifolium) as an alternate host for the wheat stripe rust pathogen (puccinia striiformis f. sp. tritici) under artificial inoculation. Plant Disease, 97(6): 839.
[8] 赵杰,张宏昌,姚娟妮,等. 中国小麦条锈菌转主寄主小檗的鉴定[J]. 菌物学报, 2011,(30):895-900.
   ZHAO Jie, ZHANG Hong-chang, YAO Juan-ni, et al. (2011). Confirmation of Berberis spp. as alternate hosts of Puccinia striiformis f. sp. tritici on wheat in China[J].Mycosystema, (30): 895-900. (in Chinese)
[9] 康振生,王晓杰,赵杰,等. 小麦条锈菌致病性及其变异研究进展[J]. 中国农业科学,2015,48(17):3 439-3 453.
   KANG Zhen-sheng, WANG Xiao-jie, ZHAO Jie, et al. (2015). Advances in research of pathogenicity and virulence variation of the wheat stripe rust fungus Puccinia striiformis f. sp. tritici. [J]. Scientia Agricultura Sinica, 48 (17): 3,439-3,453. (in Chinese)
[10] Wan, A., Zhao, Z., Chen, X., He, Z., Jin, S., & Jia, Q., et al. (2004). Wheat stripe rust epidemic and virulence of puccinia striiformis f. sp. tritici in china in 2002. Plant Disease, 88(8): 896-904.
[11] Line, R. F., & Qayoum, A. (1992). Virulence, aggressiveness, evolution and distribution of races of puccinia striiformis (the cause of stripe rust of wheat) in North America, 1968-87. Technical Bulletin.
[12] Mogens S. Hovmøller, & Karen E. Henriksen. (2008). Application of pathogen surveys, disease nurseries and varietal resistance characteristics in an ipm approach for the control of wheat yellow rust. European Journal of Plant Pathology, 121(3):377-385.
[13] 兰茗清, 刘林, 杨静, 等. 直接、快速地从罹病小麦叶片中提取条锈菌基因组 DNA的方法. 云南农业大学学报, 2012,27(6), 798-801.
   LAN Ming-qing, LIU Lin, YANG Jing, et al. (2012). Direct DNA extraction method of Puccinia striiformis f. sp. tritici from infected wheat leaves [J]. Journal of Yunnan Agricultural University, 27 (6): 798-801. (in Chinese)
[14] 中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京: 科学出版社, 2001.
   Chinese Flora Editorial Board, Chinese Academy of Sciences. (2001). Chinese Flora [M]. Beijing: Science Press. (in Chinese)
[15] Yue, J. (2011). Role of berberis spp. as alternate hosts in generating new races of puccinia graminis and p. striiformis. Euphytica, 179(1): 105-108.
[16] Roelfs, A. P. (1982). Effects of barberry eradication on stem rust in the United States. Plant Disease, 66(1): 177.

伊犁河谷小麦条锈病菌源地调查研究

Investigation into Wheat Stripe Rust Pathogen in the Valley of Yili River of Xinjiang, China

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

[1]	杨茹薇, 刘易, 李江涛, 古丽米拉·热合木土拉, 罗正乾, 徐琳黎, 沈洪飞. 马铃薯病毒病病原RT-PCR分子鉴定[J]. 新疆农业科学, 2023, 60(12): 3032-3040.
[2]	白剑宇, 罗达, 刘正兴, 李宏. 枣树花叶病毒病的分子检测[J]. 新疆农业科学, 2022, 59(3): 657-662.
[3]	路子峰, 时晓磊, 李玉姗, 崔燕华, 仙鹤, 严勇亮, 张金波, 高海峰, 丛花. 153份新疆冬小麦品种3个条锈病抗性基因的分子检测[J]. 新疆农业科学, 2022, 59(12): 2924-2932.
[4]	王冬梅, 潘洪生, 李海强, 丁瑞丰, 阿克旦・吾外士, 刘建, 李号宾. DNA分子检测技术定量评估捕食性天敌对棉蚜的控害功能[J]. 新疆农业科学, 2022, 59(11): 2661-2667.
[5]	孙晓军, 周婷婷, 玉山江·麦麦提, 何伟, 罗文芳, 许建军, 阿布都赛买提·吐尔送. 设施番茄病毒病病原鉴定[J]. 新疆农业科学, 2021, 58(1): 99-106.
[6]	白剑宇, 毕司进, 宋峰惠, 史彦江. 阿克苏地区枣黑斑病菌 PCR 检测方法的建立与应用[J]. 新疆农业科学, 2018, 55(9): 1674-1681.
[7]	高海峰, 白微微, 陈利, 杨安沛, 张航, 雷钧杰, 黄天荣, 李广阔, 王锁牢. 新疆小麦条锈病和白粉病越夏调查[J]. 新疆农业科学, 2017, 54(9): 1670-1678.
[8]	周登攀;郝小军;向本春. 新疆石河子地区设施樱桃病毒病害调查及病原检测[J]. , 2017, 54(4): 715-724.
[9]	张学军;季娟;翟文强;李寐华;王豪杰;伊鸿平. 新疆地区厚皮甜瓜白粉病菌生理小种的鉴定[J]. , 2013, 50(8): 1450-1455.
[10]	高静涛;牛建新;王雪莲;刘娜;叶春秀;张飞;朱天丁. 枣疯病植原体的分子检测与同源性鉴定[J]. , 2011, 48(4): 662-667.
[11]	王华;高峰;吴彩兰;战勇;张军;李国英. 新疆大豆疫霉生物学特性及生理小种鉴定[J]. , 2008, 45(1): 126-129.