海岛棉抗枯萎病性状与 SSR标记的关联分析

doi:10.6048/j.issn.1001-4330.2022.10.004

摘要/Abstract

摘要：

【目的】运用SSR( Simple sequence repeat) 分子标记关联分析204份海岛棉种质资源的抗枯萎病性状,为抗枯萎病棉花育种材料的利用、分子标记辅助选择提供参考。【方法】采用前期实验室研究的抗病基因序列,设计出40对SSR分子标记,采用聚丙烯酰胺凝胶电泳检测筛选标记引物特异性,并鉴定204份海岛棉种质资源。【结果】(1)从40对引物中筛选出6对引物具有多态性,多次重复后选用CHS-04和CHS-05两个分子标记,均来自类黄酮代谢途径;(2)引物CHS-04扩增条带与群体抗病性一致率介于41.67%~52.94%,平均值为46.67%;引物CHS-05扩增条带与群体材料一致率介于30.39%~54.68%,平均值为35.78%。【结论】引物CHS-04和CHS-05可以作为海岛棉枯萎病抗性的分子标记鉴定指标。

关键词: 海岛棉; 枯萎病; 关联分析; 分子标记; 引物筛选

Abstract:

【Objective】 To provide reference for the utilization of Fusarium wilt resistant cotton breeding materials and marker-assisted selection by carrying out SSR (Simple Sequence Repeat) molecular marker association analysis on 204 island cotton germplasm resources.【Method】 40 pairs of SSR markers were designed based on the disease resistance gene sequences studied in the previous laboratory.The specificity of marker primers was screened by polyacrylamide gel electrophoresis, and 204 samples of island cotton germplasm resources were identified.【Results】 (1) Six pairs of primers were selected from 40 pairs of primers with polymorphism, and two molecular markers, CHS-04 and CHS-05, were selected after repeated repeats, both from the flavonoid metabolic pathway; (2) Molecular markers and laboratory identification of 204 germplasm were used to identify Fusarium wilt.The results of comprehensive analysis showed that the primers CHS-04 amplified bands had a consistent rate of 41.67%-52.94% with an average of 46.67%.The coincidence rate of the amplified band of CHS-05 primer and the population material ranged from 30.39% to 54.68% with an average of 35.78%.【Conclusion】 Primers CHS-04 and CHS-05 can be used as molecular markers for identification of Fusarium wilt resistance of Sea Island Cotton.

Key words: sea island cotton; blight; association analysis; molecular markers; primer selection

中图分类号:

S562

陆小双, 张梦洁, 韩万里, 龙遗磊, 刘鹏飞, 陈全家, 曲延英, 邓晓娟. 海岛棉抗枯萎病性状与 SSR标记的关联分析[J]. 新疆农业科学, 2022, 59(10): 2365-2373.

LU Xiaoshuang, ZHANG Mengjie, HAN Wanli, LONG Yilei, LIU Pengfei, CHEN Quanjia, QU Yanying, DENG Xiaojuan. Association Analysis of Fusarium wilt Resistance Traits and SSR Markers in Island Cotton[J]. Xinjiang Agricultural Sciences, 2022, 59(10): 2365-2373.

图/表 11

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来源地 Origin region	数量(份) Number	来源地 Origin region	数量(份) Number
中国新疆 Xinjiang China	47	美国 America	15
中国内地 China's inland	52	埃及 Egypt	15
前苏联 Former Soviet Union	45	阿尔巴尼亚 Albania	2
未知 Unknown	27

来源地 Origin region	数量(份) Number	来源地 Origin region	数量(份) Number
中国新疆 Xinjiang China	47	美国 America	15
中国内地 China's inland	52	埃及 Egypt	15
前苏联 Former Soviet Union	45	阿尔巴尼亚 Albania	2
未知 Unknown	27

病级 Disease stage	苗期 Seedling stage
0	健株,无病状
1	子叶变黄发紫,真叶无病症
2	子叶和少数真叶发黄或发紫出现矮化病症
3	子叶和大部分真叶呈现典型病症,病叶发黄或发紫,株型矮缩或萎蔫
4	植株萎蔫,青枯死亡

病级 Disease stage	苗期 Seedling stage
0	健株,无病状
1	子叶变黄发紫,真叶无病症
2	子叶和少数真叶发黄或发紫出现矮化病症
3	子叶和大部分真叶呈现典型病症,病叶发黄或发紫,株型矮缩或萎蔫
4	植株萎蔫,青枯死亡

抗病类型 Resistance Type	病情指数 Disease Index	反应型 Reaction Type
免疫 Immune	0.0	I
高抗 Highly Resistance	0.1~5.0	HR
抗病 Resistance	5.1~10.0	R
耐病 Tolerance	10.1~20.0	T
感病 Susceptible	≥20.1	S