海岛棉GbHCT10基因的克隆与表达分析

doi:10.6048/j.issn.1001-4330.2021.02.002

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (2): 206-215.DOI: 10.6048/j.issn.1001-4330.2021.02.002

• 作物遗传育种·种质资源·分子遗传学·耕作栽培 • 上一篇下一篇

海岛棉GbHCT10基因的克隆与表达分析

闵凯丽¹, 晁祥保¹, 滕露¹, 蔡永生¹, 雷慧辰¹, 严中建¹, 郑凯^1,2, 陈全家¹

1.新疆农业大学农学院/农业生物技术重点实验室,乌鲁木齐 830052;
2.新疆农业大学博士后流动站,乌鲁木齐 830052

出版日期:2021-02-20 发布日期:2021-01-29
通信作者: 陈全家(1972-),男,江苏如皋人,教授,博士,博士生导师,研究方向为棉花遗传育种,(E-mail)chenqjia@126.com
作者简介:闵凯丽(1998-),女,本科生,研究方向为棉花遗传育种,(E-mail)1184745504@qq.com
基金资助:
国家自然科学基金“海岛棉GbHCT基因在棉花纤维发育中的功能及分子机制研究”(31860394);新疆农业大学创新项目(S201910758075);新疆农业大学作物学棉花团队发展基金“陆地棉耐低磷基因型筛选及评价指标的鉴定”(XNMH2019001);天山雪松计划(2018XS05)

Cloning and Expression Analysis of GbHCT10 Gene of Gossypium barbadense L.

MIN Kaili¹, CHAO Xiangbao¹, TENG Lu¹, CAI Yongsheng¹, LEI Huichen¹, YAN Zhongjian¹, ZHENG Kai^1,2, CHEN Quanjia¹

1. Key Laboratory of Agricultural Biological Technology / College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China;
2.Post - doctoral Mobile Station of Xinjiang Agricultural University, Urumqi 830052, China

Online:2021-02-20 Published:2021-01-29
Correspondence author: CHEN Quanjia(1972-),Male, Rugao, Jiangsu,Professor, doctor's degree, doctorial tutor, Mainly engaged in cotton genetics and breeding research,(E-mail)chenqjia@126.com
Supported by:
Supported by the National Natural Science Foundation of China “Function and Molecular Mechanism of GbHCT gene in Cotton Fiber Development ”(31860394);The Innovation Project of Xinjiang Agricultural University(S201910758075);Xinjiang Agricultural University Crop Science Cotton Group Development Fund “Screening of Low Phosphorus Tolerance Genotypes and Identification of Evaluation Indexes in Upland Cotton”(XNMH2019001);The Tianshan Cedar Project(2018XS05)

摘要/Abstract

摘要： 【目的】研究海岛棉(Gossypium barbadense)GbHCT10基因在棉纤维发育中的作用。【方法】 以海岛棉品种新海21号纤维发育第5 d的样本作为材料,根据海岛棉GbHCT10基因序列设计一对引物,利用RT-PCR技术从中克隆GbHCT10核苷酸序列。利用生物信息学方法分析GbHCT10基因序列。【结果】 采用实时荧光定量PCR技术分析海岛棉GbHCT10基因在新海21号棉花开花后不同时期中表达量差异,该基因具有长度为1 248 bp的开放阅读框,编码415个氨基酸,预测蛋白质分子量为45.13 kD,等电点为6.01。海岛棉GbHCT10基因的表达随棉纤维发育变化而变化,在开花后第5和第10 d时表达量较高,第25 d表达量最低,总体表达量呈现波动状态。【结论】 新海21号GbHCT10基因与树棉HCT基因在同一分支,有较近亲缘关系。

关键词: 海岛棉; GbHCT10; 基因克隆; 表达分析

Abstract: 【Objective】 To investigate the role of GbHCT10 gene in fiber development of Gossypium barbadense L.【Methods】 The experiment was carried out on the 5 th day of fiber development of cottonvariety Xinhai 21, a pair of primers were designed according to the sequence of GbHCT10 gene of Gossypium barbadense L., and the nucleotide sequence of GbHCT10 was cloned by RT-PCR. The analysis of the sequence of GbHCT10 gene by the bioinformatics method showed that the gene had a length of 1,248 bp, and it encoded 415 amino acids. The predicted molecular weight of the protein was 45.13 kD, and the isoelectric point was 6.01, respectively. Real-time fluorescence quantitative PCR was used to analyze the difference of GbHCT10 gene expression in Xinhai 21 cotton at different flowering stages.【Results】 The results showed that, the expression of GbHCT10 gene in Gossypium barbadense L. varied with the development of cotton fiber, and the expression was higher on the 5 th and the 10 th day after anthesis, and the expression was the lowest on the 25 th day. Evolutionary tree analysis showed that Xinhai 21 GbHCT10 gene was closely related to Gossypium arboreum HCT gene in the same branch.【Conclusion】 The results of this study las a foundation for further study on the role of GbHCT10 gene in cotton fiber development.

Key words: Gossypium barbadense L.; GbHCT10; gene cloning; expression analysis

中图分类号:

S562

闵凯丽, 晁祥保, 滕露, 蔡永生, 雷慧辰, 严中建, 郑凯, 陈全家. 海岛棉GbHCT10基因的克隆与表达分析[J]. 新疆农业科学, 2021, 58(2): 206-215.

MIN Kaili, CHAO Xiangbao, TENG Lu, CAI Yongsheng, LEI Huichen, YAN Zhongjian, ZHENG Kai, CHEN Quanjia. Cloning and Expression Analysis of GbHCT10 Gene of Gossypium barbadense L.[J]. Xinjiang Agricultural Sciences, 2021, 58(2): 206-215.

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海岛棉GbHCT10基因的克隆与表达分析

Cloning and Expression Analysis of GbHCT10 Gene of Gossypium barbadense L.

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