刺山柑果实醇提物正丁醇部位提取物抗炎镇痛效果分析

doi:10.6048/j.issn.1001-4330.2021.01.019

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (1): 159-167.DOI: 10.6048/j.issn.1001-4330.2021.01.019

• 植物保护·土壤肥料·贮藏保鲜加工·农业水土工程·微生物·农产品分析检测 • 上一篇下一篇

刺山柑果实醇提物正丁醇部位提取物抗炎镇痛效果分析

张亚涛, 包晓玮, 曾兰君, 魏晨业, 何梦梦, 金渭荃

新疆农业大学食品科学与药学学院,乌鲁木齐 830052

收稿日期:2019-02-15 出版日期:2021-01-20 发布日期:2021-01-20
通信作者: 包晓玮(1969-),女,新疆乌鲁木齐人,副教授,硕士生导师,研究方向为微生物学与免疫学,(E-mail)1546679412@qq.com
作者简介:张亚涛(1992-),男,河南鹿邑人,硕士研究生,研究方向为食品科学与工程,(E-mail)1810116636@qq.com
基金资助:
国家自然科学基金地区科学基金 (81860766)

Anti-inflammatory and Analgesic Effects of Alcohol Extracts from Capers Fruits and N-butanol Extracts

ZHANG Yatao, BAO Xiaowei, ZENG Lanjun, WEI Chenye, HE Mengmeng, JIN Weiquan

College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China

Received:2019-02-15 Online:2021-01-20 Published:2021-01-20
Correspondence author: BAO Xiaowei (1969-), female, associate professor, Urumqi, master tutor, research direction for Microbiology and Immunology, (E-mail)1546679412@qq.com
Supported by:
The National Natural Science Foundation of China (81860766)

摘要/Abstract

摘要： 【目的】 研究刺山柑乙醇提取物正丁醇萃取部位的抗炎镇痛活性。【方法】 以总黄酮提取量为指标采用超声波辅助提取法并结合响应面优化乙醇提取物最佳工艺,将乙醇提取物浸膏通过萃取得到正丁醇部位。采用二甲苯致小鼠耳廓肿胀、小鼠腹腔毛细血管通透性分析正丁醇萃取部位抗炎效果;以热板法和醋酸扭体考察正丁醇萃取部位镇痛效果。【结果】 正丁醇部位高剂量组、中剂量组对二甲苯致小鼠耳廓肿胀有明显抑制作用(P＜0.01);正丁醇部位高剂量组、中剂量组对小鼠毛细血管通透性有明显抑制作用(P＜0.01);正丁醇部位高剂量组、中剂量组可显著提高小鼠热板痛阈值,正丁醇部位高剂量组、中剂量组能明显减少醋酸所致小鼠扭体次数(P＜0.01)。【结论】 刺山柑果实醇提物正丁醇部位提取物具有潜在的抗炎镇痛作用。

关键词: 刺山柑果实; 正丁醇部位; 抗炎镇痛

Abstract: 【Objective】 To investigate the anti-inflammatory and analgesic activity of ethanol extracts of Capparis spinosa and n-butanol. 【Method】 Using ultrasonic-assisted extraction method combined with response surface to optimize the optimal ethanol extraction process with the total flavonoids extraction as the index, extract the ethanol extract to obtain the n-butanol fraction. The anti-inflammatory effect of the n-butanol extraction site was investigated using xylene-induced swelling of the auricle of mice and the capillary permeability of the abdominal cavity of the mice; the analgesic effect of the n-butanol extraction site was examined by hot plate method and twisted acetate. 【Results】 The high-dose and medium-dose groups of n-butanol significantly inhibited the auricle swelling induced by xylene in mice (P<0.01); Permeability has a significant inhibitory effect (P<0.01); high-dose and mid-dose groups in the n-butanol area could significantly increase the hot plate pain threshold in mice, and high-dose and middle-dose groups in the n-butanol area could significantly reduce the number of writhing in mice induced by acetic acid (P<0.01). 【Conclusion】 The n-butanol extract of Capparis spinosa fruit has potential anti-inflammatory and analgesic effects.

Key words: capparis fruits; N-butanol site; anti-inflammatory and analgesic

中图分类号:

S663.9

张亚涛, 包晓玮, 曾兰君, 魏晨业, 何梦梦, 金渭荃. 刺山柑果实醇提物正丁醇部位提取物抗炎镇痛效果分析[J]. 新疆农业科学, 2021, 58(1): 159-167.

ZHANG Yatao, BAO Xiaowei, ZENG Lanjun, WEI Chenye, HE Mengmeng, JIN Weiquan. Anti-inflammatory and Analgesic Effects of Alcohol Extracts from Capers Fruits and N-butanol Extracts[J]. Xinjiang Agricultural Sciences, 2021, 58(1): 159-167.

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刺山柑果实醇提物正丁醇部位提取物抗炎镇痛效果分析

Anti-inflammatory and Analgesic Effects of Alcohol Extracts from Capers Fruits and N-butanol Extracts

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