降低样品采集制备过程中土壤硝态氮和铵态氮测定误差的方法

doi:10.6048/j.issn.1001-4330.2020.03.020

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (3): 553-561.DOI: 10.6048/j.issn.1001-4330.2020.03.020

• 植物保护·土壤肥料·农业机械·畜牧兽医 • 上一篇下一篇

降低样品采集制备过程中土壤硝态氮和铵态氮测定误差的方法

朱靖蓉^1,2,3, 马磊^1,3, 康露^1,2,3, 杨涛^2,4, 李磐^2,4, 蒲胜海^2,4, 马兴旺^2,4

1.新疆农业科学院农业质量标准与检测技术研究所,乌鲁木齐 830091;
2.农业农村部西北绿洲农业环境重点实验室,乌鲁木齐 830091;
3农业部农产品质量安全风险评估实验室(乌鲁木齐),乌鲁木齐 830091;
4.新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830091

收稿日期:2019-11-18 出版日期:2020-03-20 发布日期:2020-03-11
通信作者: 马兴旺(1970-),男,研究员,博士,博士生导师,研究方向为养分资源高效利用、面源污染防治,(E-mail)maxw@xaas.ac.cn
作者简介:朱靖蓉(1972-),女,浙江龙游人,高级实验师,硕士,研究方向为农产品营养与质量安全,(E-mail)zhujr2030@163.com
基金资助:
国家重点研发计划(2018YFD0800802,2018YFD0800804)

Review on the Method for Reducing Measurement Error of Soil Nitrate Nitrogen and Ammonium Nitrogen during Sample Collection and Preparation

ZHU Jingrong^1,2,3, MA Lei^1,2, KANG Lu^1,2,3, YANG Tao^2,4, LI Pan^2,4, PU Shenghai^2,4, MA Xingwang^2,4

1. Research Institute of Agricultural Quality Standards and Testing Technology, Xinjiang Academy of Agricultural Sciences, Urumqi , 830091, China;
2. Key Laboratory of Northwest Oasis Agricultural Environment, Ministry of Agriculture and Rural Affairs, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
3. Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture, Urumqi 830091, China;
4. Research Institute of Soil, Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2019-11-18 Online:2020-03-20 Published:2020-03-11
Correspondence author: MA Xingwang(1970-),male,born in Ningxia,Research professor, doctoral supervisor,Mainly engaged in research on efficient utilization of nutrient resources and prevention of non-point source pollution,(E-mail)maxw@xaas.ac.cn
Supported by:
Supported by the National R & D Program Projects (2018YFD0800802 and 2018YFD0800804)

摘要/Abstract

摘要： 【目的】分析新鲜土壤样品采集、保存、浸提以及浸提液存放等处置方式对土壤硝态氮、铵态氮测定结果误差的影响,研究降低测定结果误差的途径,提出实现测定结果在误差范围内的土样采集和制备方法。【方法】通过对文献收集、整理和统计分析,分析测定过程中人为影响痕迹最多的新鲜土样的采集、保存、浸提以及浸提液存放等环节的处置方式对土壤硝态氮、铵态氮测定结果误差的影响。【结果】实验室测定步骤有严格误差控制,而土壤样品采集和制备环节易引起测定数据误差;土样采集和运输时放置在室外或低温运输都会引起土壤硝态氮和铵态氮含量增加;土样风干、烘干保存时硝态氮和铵态氮含量增加,长时间冷藏含量也会增加,冷冻保存时含量变化最小;不同浸提剂和不同浸提时间的测定结果有差异,浸提液保存方式对测定结果有明显影响。【结论】测定得到接近土样采集时的田间新鲜土壤中氮含量的关键途径为加快采样速度、样品储运、浸提等环节。

关键词: 土壤; 硝态氮; 铵态氮; 前处理; 采样

Abstract: 【Objective】 To analyze the effects of the disposal methods of fresh soil sample collection, preservation, extraction and storage of extract on the error of soil nitrate nitrogen and ammonium nitrogen determination results, and explore the ways to reduce errors in measurement results. 【Methods】 Through literature collection, sorting and statistical analysis, the effects of methods of collection, preservation, extraction and storage of leaching liquid on the error of soil nitrate and ammonium nitrogen determination results were comprehensively analyzed. 【Results】 It was so easy to cause the measurement data error that the laboratory measurement procedure had strict error control, and the soil sample collection and preparation link. When soil samples were collected and transported outdoors or at low temperature, the contents of nitrate and ammonium nitrogen in soil would increase. When soil samples were dried by wind or by heat and preserved, the content of nitrate nitrogen and ammonium nitrogen increased. The contents of them by cold storage for a long time would increase, but the change was the least, and the determination results of different extractants and different extraction time would be different, and the way of extraction solution preservation would have a significant effect on the results. 【Conclusion】 The key way to determine the nitrogen content in the fresh soil is to accelerate the sampling speed, sample storage and transportation, extraction and so on. In addition, the method of collecting and preparing soil samples within the error range was also proposed.

Key words: soil; nitrate nitrogen; ammonium nitrogen; pretreatment; sampling

中图分类号:

S14-33

朱靖蓉, 马磊, 康露, 杨涛, 李磐, 蒲胜海, 马兴旺. 降低样品采集制备过程中土壤硝态氮和铵态氮测定误差的方法[J]. 新疆农业科学, 2020, 57(3): 553-561.

ZHU Jingrong, MA Lei, KANG Lu, YANG Tao, LI Pan, PU Shenghai, MA Xingwang. Review on the Method for Reducing Measurement Error of Soil Nitrate Nitrogen and Ammonium Nitrogen during Sample Collection and Preparation[J]. Xinjiang Agricultural Sciences, 2020, 57(3): 553-561.

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降低样品采集制备过程中土壤硝态氮和铵态氮测定误差的方法

Review on the Method for Reducing Measurement Error of Soil Nitrate Nitrogen and Ammonium Nitrogen during Sample Collection and Preparation

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