高效液相色谱法测定不同品种石榴皮中5种类黄酮的含量

doi:10.6048/j.issn.1001-4330.2019.09.011

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (9): 1659-1667.DOI: 10.6048/j.issn.1001-4330.2019.09.011

• 园艺·林业·生理生化 • 上一篇下一篇

高效液相色谱法测定不同品种石榴皮中5种类黄酮的含量

孟新涛^{1, 2}, 魏健³, 许铭强^{1, 2}, 李德华⁴, 谭归⁴, 朱丽娜⁴, 邵文志⁴, 潘俨^{1, 2}

1.新疆农业科学院农产品贮藏加工研究所, 乌鲁木齐 830091;
2.新疆主要农副产品精深加工工程技术研究中心,乌鲁木齐 830091;
3.喀什大学生命与地理科学学院,新疆喀什 844006;
4.新疆农业大学食品科学与药学学院,乌鲁木齐 830052

收稿日期:2019-06-09 发布日期:2019-10-30
通信作者: 潘俨(1979-),男,副研究员, 博士研究生,研究方向为农产品贮藏加工与保鲜,(E-mail)panyan1590@163.com
作者简介:孟新涛(1985-),女,硕士研究生,助理研究员,研究方向为农产品贮藏加工与保鲜,(E-mail)mengxintao_929@126.com
基金资助:
国家科技支撑计划(2011BAD27B01-01-02);新疆农业科学院青年基金(xjnkq-2015038)

Determination of Five Flavonoids in Pomegranate Peel of Different Cultivars by High Performance Liquid Chromatography

MENG Xin-tao^{1, 2}, WEI Jian³, XU Ming-qiang^{1, 2}, LI De-hua⁴, TAN Gui⁴, ZHU Li-na⁴, SHAO Wen-zhi⁴, PAN Yan^{1, 2}

1.Research Institute of Agro-products Storage and Processing, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091, China;
2. Research Center of Main Subsidiary Agro-products Processing Engineering in Xinjiang, Urumqi, 830091, China;
3. College of Life and Geography Sciences, Kashi University, Kashi , Xinjiang 844006, China;
4. College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China

Received:2019-06-09 Published:2019-10-30
Correspondence author: PAN Yan(1979-),male,doctoral candidate,research associate,engaged in farm product storage and processing, (E-mail)panyan1590@163.com
Supported by:
Supported by the National Science and Technology Support Project (2011BAD27B01-01-02) and Outstanding Young Scientific Talent Foundation of Xingjiang Academy of Agricultural Sciences(xjnkq-2015038)

摘要/Abstract

摘要： 【目的】研究新疆石榴皮中5种类黄酮物质的测定方法,分析不同品种石榴果皮中类黄酮物质的组分及含量,为新疆石榴类黄酮物质的良种选育和综合开发利用提供理论依据。【方法】以新疆主栽4个石榴品种皮亚曼、千籽红、赛柠檬和叶城石榴为试材,采用高效液相色谱法(HPLC)分别测定其果皮中芦丁、槲皮素、槲皮苷、山奈酚和异鼠李素5种类黄酮物质含量。【结果】石榴果皮中类黄酮含量测定的液相色谱条件:C₁₈(Agilent Poroshell 120 EC-C₁₈柱,250 mm× 4.6 mm,孔径4 μm)色谱柱;流动相:100%甲醇:0.2%甲酸=55∶45,紫外检测器(G1362A RID),检测波长:360 nm。5种类黄酮在21 min内完全分离,线性范围为1~150 μg/mL(R²=0.991 9-0.999 7),回收率为76.68%~124.50%,检出限为0.2 mg/kg,定量限为5 mg/kg。4个品种中均检出芦丁、槲皮素、槲皮苷、山奈酚和异鼠李素5种类黄酮物质,其中槲皮苷含量最高。【结论】 4个品种石榴皮提取物中芦丁、槲皮苷、槲皮素、异山奈酚和李素含量差异较大,由高到低依次为:皮亚曼＞赛柠檬＞千籽红＞叶城石榴。

关键词: 石榴; 果皮; 类黄酮; 高效液相色谱法

Abstract: 【Objective】 This study aims to investigate a high-performance liquid chromatograph(HPLC)method for the determination of different kinds of flavonoids and research and analyze the composition and content of flavonoids in pomegranate pericarp of different varieties in Xinjiang in the hope of providing theoretical basis for the breeding and comprehensive development and utilization of pomegranate flavonoids in Xinjiang. 【Method】'Piyaman', 'Qianzihong', 'Saining meng' and'Yechen Pomegranate' were used as the test materials, five kinds of flavonoids: rutin, meletin, quercetin, rhizoma kaempferiae phenol and isorhamnetin were analyzed by HPLC method. The chromatographic separation was performed on Agilent Poroshell 120 EC-C18 (250 mm×4.6 mm,4 μm) using methanol/formic acid/water mixture as the mobile phase at a flow rate of 0.5 mL·min-1through gradient elution, and the detection wavelength was set at 360 nm. 【Result】Results showed that the thorough separation of 5 flavonoids was achieved within 21 min. The method exhibited a linear range of 1-150 μg/mL(R²=0.991,9-0.999,7)and detection limits was 0.2 mg/kg, quantitative limit was 5 mg/kg, the average recovery rates of this method were 76.68%~124.50%. 5 kinds of flavonoids such as rutin, meletin, quercetin, rhizoma kaempferiae phenol and isorhamnetin were detected in all four varieties, among which, quercetin was the highest. 【Conclusion】 There were differences in FLs content between the four different cultivars. 'Piyaman' had the highest total FLs content in peel. From high to low : 'Piyaman'＞'Sainingmeng' ＞'Qianzihong'＞'Yechen Pomegranate'.

Key words: pomegranate; peel; juice; flavonoids; HPLC

中图分类号:

TS255.1

孟新涛, 魏健, 许铭强, 李德华, 谭归, 朱丽娜, 邵文志, 潘俨. 高效液相色谱法测定不同品种石榴皮中5种类黄酮的含量[J]. 新疆农业科学, 2019, 56(9): 1659-1667.

MENG Xin-tao, WEI Jian, XU Ming-qiang, LI De-hua, TAN Gui, ZHU Li-na, SHAO Wen-zhi, PAN Yan. Determination of Five Flavonoids in Pomegranate Peel of Different Cultivars by High Performance Liquid Chromatography[J]. Xinjiang Agricultural Sciences, 2019, 56(9): 1659-1667.

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高效液相色谱法测定不同品种石榴皮中5种类黄酮的含量

Determination of Five Flavonoids in Pomegranate Peel of Different Cultivars by High Performance Liquid Chromatography

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