新疆野生甜瓜形态学观察及其与不同变种杂交亲和性研究

doi:10.6048/j.issn.1001-4330.2017.09.008

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (9): 1627-1635.DOI: 10.6048/j.issn.1001-4330.2017.09.008

新疆野生甜瓜形态学观察及其与不同变种杂交亲和性研究

李寐华^1,2, 樊国全³, 杨永¹, 王广智¹, 张建⁴, 张学军¹, 伊鸿平¹, 张永兵¹

1.新疆农业科学院哈密瓜研究中心,乌鲁木齐 830091;
2.新疆农业大学林学与园艺学院,乌鲁木齐 830052;
3.新疆农业科学院科研管理处,乌鲁木齐 830091;
4.浙江衢州新农都实业有限公司,浙江衢州 324022

收稿日期:2017-03-19 发布日期:2020-07-22
通信作者: 张永兵(1971-),男,河南临颍人,研究员,博士,研究方向为甜瓜遗传育种与生物技术,(E-mail)luoybzhang@163.com
作者简介:李寐华(1985-),男,湖南湘潭人,助理研究员,研究方向为西瓜甜瓜遗传育种,(E-mail)mh0732@qq.com
基金资助:
国家自然科学基金项目“新疆甜瓜种质资源遗传多样性及野生甜瓜系统生物学研究”(31260480);国家现代农业产业技术体系建设专项“厚皮甜瓜育种岗”(CARS-26-04);“十二五”农村领域国家科技计划研究任务“西甜瓜杂种优势利用与新品种选育”(2012BAD02B03-4)

Morphological Observation on Xinjiang Wild Muskmelons and Study on Cross Compatibility between Them and Different Varieties in Cucumis melo L.

LI Mei-hua^1,2, FAN Guo-quan³, YANG Yong¹, WANG Guang-zhi¹, ZHANG Jian⁴, ZHANG Xue-jun¹, YI Hong-ping¹, ZHANG Yong-bing¹

1. Hami Melon Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
2. College of Forestry and Horticulture,Xinjiang Agricultural University,Urumqi 830052,China;
3. Scientific Research Management Office, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
4. Zhejiang Quzhou Xinnongdu Industry Co., Ltd., Quzhou Zhejiang 324022, China

Received:2017-03-19 Published:2020-07-22
Correspondence author: ZHANG Yong-bing (1971-), male, native place: Linying Henan. Research Fellow. Ph.D, research field: Genetics, breeding and biotechnology of melon, (E-mail)luoybzhang@163.com
Supported by:
National Natural Science Foundation of China "Study on Genetic Diversity of Melon (Cucumis melo L.) Germplasm and Biological Characteristics of Wild Melon in Xinjiang" (31260480); Modern Agro-industry Technology Research System "Position of Breeding on Muskmelon"(CARS-26-04); National Science & Technology Pillar Program during the 12th Five-year Plan Period "Utilization of Heterosis and New Cultivar Selection of Muskmelon and Watermelon"(2012BAD02B03-4)

摘要/Abstract

摘要： 【目的】通过形态学观察鉴定与种内不同变种间杂交亲和性研究,为明确新疆野生甜瓜分类地位和扩大甜瓜栽培品种遗传基础提供支撑。【方法】观察比较两份新疆野生甜瓜和地方品种叶片、茎、花及果实等28个形态学性状,利用苗期接种技术鉴定新疆野生甜瓜对蔓枯病和白粉病的抗病性。同时,以亚种ssp. agrestis内var. agrestis、var. conomon、var. dudaim、var. chito和var. momordica等5个变种,亚种ssp. melo内var. cantalupensis、var. inodorus和var. flexuosus等3个变种为试材,通过人工授粉杂交后坐果率和结籽率的观察记录,分析新疆野生甜瓜与种内的8个变种间的杂交亲和性。【结果】哈密野甜瓜绝大多数性状与栽培品种对照差异明显,而吐鲁番野甜瓜大部分性状与对照接近。苗期人工接种甜瓜蔓枯病和白粉病菌后,哈密野甜瓜高抗蔓枯病、不抗白粉病,吐鲁番野甜瓜和对照易感或高感蔓枯病和白粉病。人工授粉杂交后,新疆野生甜瓜与2个亚种的8个变种之间未发现不亲和不稔现象;野生甜瓜与8个变种杂交果实的结籽率正反交间有差异,以野生甜瓜为母本时的结籽率高于父本时的;此外,哈密野甜瓜与亚种ssp. agrestis和ssp. melo杂交的平均结籽率分别为79.3%和83.4%,吐鲁番野甜瓜与两个亚种杂交的平均结籽率分别为76.1%和79.4%,野生甜瓜与两个亚种杂交的平均结籽率相近。【结论】新疆野生甜瓜哈密野甜瓜的形态性状与亚种ssp. agrestis内野生变种的性状相近,而吐鲁番野甜瓜的形态性状接近于地方栽培品种。经苗期接种鉴定,哈密野甜瓜对甜瓜蔓枯病表现高抗。新疆野生甜瓜与甜瓜两个亚种ssp. agrestis和ssp. melo可正常杂交且杂种后代可育,应当被划分在C. melo.内。

关键词: 野生甜瓜; 形态学; 结籽率; 杂交亲和性

Abstract: 【Objective】 Through morphological observation, identification and cross compatibility between wild muskmelon and varieties in Cucumis melo L. this project aims to provide a support for the classification of wild muskmelons in Xinjiang and the genetic base of melon cultivars.【Method】Twenty-eight traits of leaf, stem, flower and fruit of Xinjiang wild melon were observed and compared with local landraces, and the disease resistance of wild melon in Xinjiang to 2 fungi diseases: gummy stem blight (GSB) and powdery mildew was identified by inoculation technique at the seedling stage. Meanwhile, cross compatibility between Xinjiang wild melon and 8 varieties, in which 5 were included in ssp. agrestis, var. agrestis, var. conomon, var. dudaim, var. chito and var. momordica, and 3 were included in ssp. melo, var. cantalupensis, var. inodorus and var. flexuosus were analyzed, by the way of recording on fruit-setting and seed-setting rate of combinations after artificial pollination.【Result】Results obtained showed that most morphological traits of 'Hami wild' were distinct from the local cultivated control, but traits of 'Turpan wild' were similar to the control. 'Hami wild' was highly resistant to GSB but susceptible to PM, while 'Turpan wild' was susceptible or highly susceptible to GSB and PM after inoculation at seedling stage. Infertility of hybrids and incompatibility were not observed among any combinations between Xinjiang wild melon and 8 varieties. Except var. agrestis and chito, the seed-setting rate between reciprocal crosses was found differentially among combinations between Xinjiang wild melon and 8 varieties, which higher seed-setting rate was detected when Xinjiang wild melon was used as female parent. Furthermore, the seed-setting rate between 'Hami wild' and 2 subspecies, ssp. agrestis and ssp. melo, were 79.3% and 83.4%, respectively; and the seed-setting rate between 'Turpan wild' and 2 subspecies were 76.1% and 79.4%, respectively, which indicated that seed-setting rate exhibited small differences between combinations of Xinjiang wild melon with ssp. agrestis and with ssp. melo. 【Conclusion】Morphological traits observed in 'Hami wild' looked like that of feral or weedy melon in ssp. agrestis, however, 'Turpan wild' showed affinities with local landraces. 'Hami wild' was highly resistant to GSB after screening in seedlings. Xinjiang wild melon could be classified into C. melo, because of fertility of hybrids and compatibility were detected between wild melon and 2 subspecies, ssp. agrestis, ssp. melo.

Key words: wild melon; morphology; seed-setting rate; cross compatibility

中图分类号:

S652.9

李寐华, 樊国全, 杨永, 王广智, 张建, 张学军, 伊鸿平, 张永兵. 新疆野生甜瓜形态学观察及其与不同变种杂交亲和性研究[J]. 新疆农业科学, 2017, 54(9): 1627-1635.

LI Mei-hua, FAN Guo-quan, YANG Yong, WANG Guang-zhi, ZHANG Jian, ZHANG Xue-jun, YI Hong-ping, ZHANG Yong-bing. Morphological Observation on Xinjiang Wild Muskmelons and Study on Cross Compatibility between Them and Different Varieties in Cucumis melo L.[J]. Xinjiang Agricultural Sciences, 2017, 54(9): 1627-1635.

参考文献

[1] Decker-Walters, D. S., Chung, S. M., Staub, J. E., Quemada, H. D., & López-Sesé, A. I. (2002). The origin and genetic affinities of wild populations of melon ( cucumis melo, cucurbitaceae) in North America. Plant Systematics & Evolution, 233(3-4): 183-197.
[2] Roy A, Bal S S, Fergany M, Kaur S, Singh H, Malik A A, Singh J, Monforte A J, Dhillon N P S. (2012). Wild melon diversity in India (Punjab State). Genetic Resources & Crop Evolution, (59): 755-767.
[3] Pitrat, M. (2013). Phenotypic diversity in wild and cultivated melons (cucumis melo). Plant Biotechnology, 30(3): 273-278.
[4] John, K. J., Scariah, S., Nissar, V. A. M., Latha, M., Gopalakrishnan, S., & Yadav, S. R., et al. (2013). On the occurrence, distribution, taxonomy and genepool relationship of cucumis callosus (rottler) cogn. the wild progenitor of cucumis melo l. from india. Genetic Resources & Crop Evolution, 60(3):1,037-1,046.
[5] Zhang, C., Pratap, A. S., Natarajan, S., Pugalendhi, L., Kikuchi, S., & Sassa, H., et al. (2012). Evaluation of morphological and molecular diversity among south asian germplasms of cucumis sativus and cucumis melo. ISM Agronomy, doi: 10.5402/2012/134134.
[6] Trimech, R., Zaouali, Y., Boulila, A., Chabchoub, L., Ghezal, I., & Boussaid, M. (2013). Genetic variation in tunisian melon (cucumis melo, L.) germplasm as assessed by morphological traits. Genetic Resources & Crop Evolution, 60(5):1,621-1,628.
[7] Hu, J., Wang, P., Su, Y., Wang, R., Li, Q., & Sun, K. (2015). Microsatellite diversity, population structure, and core collection formation in melon germplasm. Plant Molecular Biology Reporter, 33(3): 439-447.
[8] Tanaka, K., Stevens, C. J., Iwasaki, S., Akashi, Y., Yamamoto, E., & Dung, T. P., et al. (2016). Seed size and chloroplast dna of modern and ancient seeds explain the establishment of japanese cultivated melon (cucumis melo, L.) by introduction and selection. Genetic Resources & Crop Evolution, 63(7):1,237-1,254.
[9] Stepansky, A., Kovalski, I., & Perl-Treves, R. (1999). Intraspecific classification of melons (cucumis melo, L.) in view of their phenotypic and molecular variation. Plant Systematics & Evolution, 217(3-4): 313-332.
[10] Nesom, G. L. (2011). Toward consistency of taxonomic rank in wild/domesticated cucurbitaceae. Phytoneuron. (13):1-33.
[11] Hammer, K., & Gladis, T. (2014). Notes on infraspecific nomenclature and classifications of cultivated plants in compositae, cruciferae, cucurbitaceae, gramineae (with a remark on triticum dicoccon, schrank) and leguminosae. Genetic Resources & Crop Evolution, 61(8):1,455-1,467.
[12] Ali-Shtayeh, M. S., Jamous, R. M., Shtaya, M. J., Mallah, O. B., Eid, I. S., & Zaitoun, S. Y. A. (2010). Morphological characterization of snake melon ( cucumis melo, var. flexuosus ) populations from palestine. Genetic Resources & Crop Evolution, accepted: 1-16.
[13] Singh, A. K., & Yadava, K. S. (1984). An analysis of interspecific hybrids and phylogenetic implications in cucumis, ( cucurbitaceae ). Plant Systematics & Evolution, 147(3-4): 237-252.
[14] Alexander Beharav, & Yigal Cohen. (1995). Attempts to overcome the barrier of interspecific hybridization between and. Israel Journal of Plant Sciences, (2): 113-123.
[15] Sebastian, P., Schaefer, H., Telford, I. R., & Renner, S. S. (2010). Cucumber (cucumis sativus) and melon (c. melo) have numerous wild relatives in asia and australia, and the sister species of melon is from australia. Proceedings of the National Academy of Sciences of the United States of America, 107(32): 14,269-14,273.
[16] Matsumoto, Y., Miyagi, M., Watanabe, N., & Kuboyama, T. (2012). Temperature-dependent enhancement of pollen tube growth observed in interspecific crosses between wild cucumis, spp. and melon ( c. melo, l.). Scientia Horticulturae, 138(138):144-150.
[17] Morales, M., Orjeda, G., Nieto, C., Leeuwen, H. V., Monfort, A., & Charpentier, M., et al. (2005). A physical map covering the nsv, locus that confers resistance to melon necrotic spot virus, in melon (cucumis melo, L.). Tag.theoretical & Applied Genetics.theoretische Und Angewandte Genetik, 111(5): 914-922.
[18] Boissot, N., Thomas, S., Sauvion, N., Marchal, C., Pavis, C., & Dogimont, C. (2010). Mapping and validation of qtls for resistance to aphids and whiteflies in melon. Theoretical & Applied Genetics, 121(1): 9-20.
[19] 咸丰,张勇,马建祥,等. 野生甜瓜'云甜-930'抗白粉病主基因+多基因遗传分析[J]. 中国农业科学,2011,44(7):1 425-1 433.
   XIAN Feng, ZHANG Yong, MA Jian-xiang, et al. (2011). Genetic Analysis of Resistant to Powdery Mildew with Mixed Modelof Major Gene Plus Polygene in Wild Melon Material 'Yuntian-930'[J]. Scientia Agricultura Sinica, 44(7):1,425-1,433. (in Chinese)
[20] Diaz, A., Fergany, M., Formisano, G., Ziarsolo, P., Blanca, J., & Fei, Z., et al. (2011). A consensus linkage map for molecular markers and quantitative trait loci associated with economically important traits in melon (cucumis melo L.). Bmc Plant Biology, 11(1): 1-14.
[21] Malik, A. A., Vashisht, V. K., Singh, K., Sharma, A., Singh, D. K., & Singh, H., et al. (2014). Diversity among melon (cucumis melo L.) landraces from the indo-gangetic plains of india and their genetic relationship with usa melon cultivars. Genetic Resources & Crop Evolution, 61(6):1,189-1,208.
[22] 王坚. 中国西瓜甜瓜[M]. 北京:中国农业出版社,2000: 355-368.
   WANG Jian. (2000). China Watermelon and Muskmelon [M]. Beijing: China Agriculture Press: 355-368. (in Chinese)
[23] 张永兵,李寐华,吴海波,等. 新疆甜瓜地方品种资源的表型遗传多样性[J]. 园艺学报,2012,(39):305-314.
   ZHANG Yong-bing, LI Mei-hua, WU Hai-bo, et al. (2012).Genetic diversity of melon landraces (Cucumis melo L.) in Xinjiang based on phenotypic characters [J]. Acta Horticulturae Sinica, (39): 305-314. (in Chinese)
[24] Renner, S. S., Schaefer, H., & Pitrat, M. (2008). Phylogenetics of Cucumis (Cucurbitaceae) as understood in 2008. Cucurbitaceae 2008. Proceedings of the Ixth Eucarpia Meeting on Genetics and Breeding of Cucurbitaceae, Avignon, France, 21-24 May.
[25] 吴明珠. 吐鲁番盆地厚皮甜瓜品种资源及其利用[J]. 中国果树,1982,(2):28-33.
   WU Ming-zhu. (1982).Germplasm resources and utilization of Muskmelon in Turpan Basin [J]. Journal of Fruits Science, 9(2): 28-33. (in Chinese)
[26] 林德佩. 新疆野生甜瓜的研究[J]. 新疆八一农学院学报,1984,(1):50-52.
   LIN De-pei. (1984).Study on wild melon (Cucumis melo L.) in Xinjiang [J]. Journal of Bayi Agricultural College, (1): 50-52. (in Chinese)
[27] Zhang, Y. P., Kyle, M., Anagnostou, K., & Zitter, T. A. (1997). Screening melon (cucumis melo) for resistance to gummy stem blight in the greenhouse and field. Hortscience A Publication of the American Society for Horticultural Science, 32(1):117-121.
[28]周晓慧,Joseph N. Wolukau,李英,等. 甜瓜抗蔓枯病种质资源的筛选及RAPD分析[J]. 园艺学报,2007,34(5):1 201-1 206.
   ZHOU Xiao-hui, JOSEPH N. Wolukau, LI Ying, et al. (2007). Screening and RAPD analysis of gummy stem blight resistance in melon germplasm [J]. Acta Horticulturae Sinica, 34(5):1,201-1,206. (in Chinese)
[29] Zhang, Y., Fan, X., Aierken, Y., Ma, X., Yi, H., & Wu, M. (2016). Genetic diversity of melon landraces ( cucumis melo, l.) in the xinjiang uygur autonomous region on the basis of simple sequence repeat markers. Genetic Resources & Crop Evolution: 1-13.
[30] Akashi, Y., Fukuda, N., Wako, T., Masuda, M., & Kato, K. (2002). Genetic variation and phylogenetic relationships in east and south asian melons, cucumis melo l. based on the analysis of five isoenzymes. Euphytica, 125(3): 385-396.
[31] Tanaka, K., Nishitani, A., Akashi, Y., Sakata, Y., Nishida, H., & Yoshino, H., et al. (2007). Molecular characterization of south and east asian melon, cucumis melo l. and the origin of group conomon var. makuwa and var. conomon revealed by rapd analysis. Euphytica, 153(1-2): 233-247.

新疆野生甜瓜形态学观察及其与不同变种杂交亲和性研究

Morphological Observation on Xinjiang Wild Muskmelons and Study on Cross Compatibility between Them and Different Varieties in Cucumis melo L.

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

[1]	裴红霞, 李生梅, 武旭霞, 耿世伟, 赖黎丽, 高晶霞, 董心久. 辣椒核雄性不育系pby-1形态学及生理生化特性分析[J]. 新疆农业科学, 2023, 60(3): 624-632.
[2]	吴巧玉, 何天久. 紫色甘薯资源的形态学和ISSR荧光标记分析[J]. 新疆农业科学, 2022, 59(7): 1625-1631.
[3]	郭书含, 罗顺刚, 钟问, 康宁, 胡红英. 中国克虎天牛属整合分类[J]. 新疆农业科学, 2022, 59(4): 957-965.
[4]	蒋玲玲, 罗顺刚, 胡红英. 新疆云粉蝶属雄性外生殖器比较形态学[J]. 新疆农业科学, 2019, 56(2): 363-372.
[5]	罗影;关永强;贾培松;努尔孜亚·亚力买买提;郝敬喆;贾文捷;魏鹏. 新疆阿魏菇细菌性斑点病病原菌的鉴定[J]. , 2017, 54(3): 513-519.
[6]	艾尼古丽·依明;付超;郭开发;何丽;赵思峰. 石河子地区寄生性杂草菟丝子的种类鉴定[J]. , 2017, 54(2): 327-335.
[7]	尹弯, 钟问, 胡红英. 新疆寡索赤眼蜂属Oligosita Walker(膜翅目:赤眼蜂科)比较形态学研究[J]. 新疆农业科学, 2017, 54(10): 1893-1902.
[8]	王开胜;刘继荣. 新疆北疆部分地区鸡球虫病病原调查及分类鉴定[J]. , 2016, 53(5): 955-960.
[9]	曹文秋;熊嘉武;王雨晴;林思雨;胡红英. 新疆七种峡蝶雄性外生殖器的比较形态学研究[J]. , 2016, 53(4): 679-687.
[10]	王金泉;何宗林;赵红琼;王小静;达拉;蔡秀莲;姚刚;刘志强. 新和鸽消化系统形态学观察与测量[J]. , 2004, 41(4): 251-254.