Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (10): 2484-2490.DOI: 10.6048/j.issn.1001-4330.2024.10.016
• Plant Protection · Soil Fertilizer · Water Saving Irrigation · Agricultural Equipment Engineering and Mechanization · Prataculture • Previous Articles Next Articles
YANG Ruwei(), LIU Yi(
), Gulimila Rehemutula, SUN Hui, JIANG Yinghong
Received:
2024-04-12
Online:
2024-10-20
Published:
2024-11-07
Correspondence author:
LIU Yi
Supported by:
杨茹薇(), 刘易(
), 古丽米拉·热合木土拉, 孙慧, 江应红
通讯作者:
刘易
作者简介:
杨茹薇(1984-),女,宁夏灵武人,正高级农艺师,研究方向为植物保护,(E-mail)617950493@qq.com
基金资助:
CLC Number:
YANG Ruwei, LIU Yi, Gulimila Rehemutula, SUN Hui, JIANG Yinghong. Research on real-time fluorescence quantitative PCR detection of potato virus disease[J]. Xinjiang Agricultural Sciences, 2024, 61(10): 2484-2490.
杨茹薇, 刘易, 古丽米拉·热合木土拉, 孙慧, 江应红. 马铃薯病毒病实时荧光定量PCR检测技术[J]. 新疆农业科学, 2024, 61(10): 2484-2490.
病毒种类 Virus type | 序列(5'-3') Sequence(5'-3') | Tm(℃) |
---|---|---|
PVY | F:TGAAAATGGAACCTCGCC | 89.3 |
R:AATGTGCCATGATTTGCC | ||
PVS | F:TCGTBTGGAATTACATGCTG | 90.6 |
R:ATCAAATGTGTCAAAGCGG | ||
PLRV | F:AAGAAGGCAATCCCTTCG | 86.7 |
R:ATGTCTCGCTTGAGCCTC |
Tab.1 Virus primer sequences
病毒种类 Virus type | 序列(5'-3') Sequence(5'-3') | Tm(℃) |
---|---|---|
PVY | F:TGAAAATGGAACCTCGCC | 89.3 |
R:AATGTGCCATGATTTGCC | ||
PVS | F:TCGTBTGGAATTACATGCTG | 90.6 |
R:ATCAAATGTGTCAAAGCGG | ||
PLRV | F:AAGAAGGCAATCCCTTCG | 86.7 |
R:ATGTCTCGCTTGAGCCTC |
引物 Primer | 标准曲线方程 Standard curve equation | R2 | 扩增效率 Amplification efficiency(%) | 斜率 Slope | Y轴截距 Y-Intercept |
---|---|---|---|---|---|
PVY | Y=-3.57X+37.05 | 0.994 2 | 90.59 | 3.57 | 37.05 |
PVS | Y=-3.76X+39.93 | 0.996 4 | 84.48 | 3.76 | 39.93 |
PLRV | Y=-3.72X+40.16 | 0.998 2 | 85.7 | 3.72 | 40.16 |
Tab.2 Standard curves of real-time fluorescence quantitative PCR for PVY, PVS, PLRV
引物 Primer | 标准曲线方程 Standard curve equation | R2 | 扩增效率 Amplification efficiency(%) | 斜率 Slope | Y轴截距 Y-Intercept |
---|---|---|---|---|---|
PVY | Y=-3.57X+37.05 | 0.994 2 | 90.59 | 3.57 | 37.05 |
PVS | Y=-3.76X+39.93 | 0.996 4 | 84.48 | 3.76 | 39.93 |
PLRV | Y=-3.72X+40.16 | 0.998 2 | 85.7 | 3.72 | 40.16 |
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