Inhibitory effect of different phases of Streptomyces rochei A144 volatile substances on Valsa mali var.mali

doi:10.6048/j.issn.1001-4330.2024.10.015

Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (10): 2475-2483.DOI: 10.6048/j.issn.1001-4330.2024.10.015

• Plant Protection · Soil Fertilizer · Water Saving Irrigation · Agricultural Equipment Engineering and Mechanization · Prataculture • Previous Articles Next Articles

Inhibitory effect of different phases of Streptomyces rochei A144 volatile substances on Valsa mali var.mali

HUANG Wei¹(), WANG Ning¹, SONG Bo², WANG Suling¹, QIN Xinzheng¹, SONG Suqin¹, LUO Yi¹, WEI Zengzhou¹, ZHANG Lijuan¹^,³(), WANG Wei¹()

1. Xinjiang Laboratory of Special Environmental Microbiology/Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China
2. Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China
3. College of Grassland Science, Xinjiang Agricultural University, Urumqi 830052,China

Received:2024-04-08 Online:2024-10-20 Published:2024-11-07
Correspondence author: ZHANG Lijuan, WANG Wei
Supported by:
The Open Project of Key Laboratory of Xinjiang Uygur Autonomous Region(2020D04032);Youth the Fund Project of Xinjiang Uygur Autonomous Region(2022D01B172);Innovation Ability Training Project for Young Sci-Tech Backbone Talents Sponsored by Xinjiang Academy of Agricultural Sciences(xjnkq-2023015);Innovation Ability Training Project for Young Sci-Tech Backbone Talents Sponsored by Xinjiang Academy of Agricultural Sciences(xjnkq-2023013)

不同相态娄彻氏链霉菌A144挥发性物质对苹果树腐烂病菌的抑制效果

黄伟¹(), 王宁¹, 宋博², 王苏玲¹, 秦新政¹, 宋素琴¹, 罗义¹, 魏增宙¹, 张丽娟¹^,³(), 王玮¹()

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091
2.新疆农业科学院植物保护研究所,乌鲁木齐 830091
3.新疆农业大学草业学院,乌鲁木齐 830000

通讯作者: 张丽娟,王玮
作者简介:黄伟(1991-),男,新疆阿图什人,助理研究员,研究方向为有益微生物代谢产物,(E-mail)1428775150@qq.com
基金资助:
新疆维吾尔自治区重点实验室开放课题(2020D04032);新疆维吾尔自治区青年基金项目(2022D01B172);新疆农业科学院青年骨干创新能力培养项目(xjnkq-2023015);新疆农业科学院青年骨干创新能力培养项目(xjnkq-2023013)

Abstract

Abstract:

【Objective】 To investigate the inhibitory effect of volatile substances produced by Streptomyces rochei A144 on Valsa mali under different phases, and to detect and determine the volatile substance with antifungal activity in vitro. 【Methods】 The inhibitory effect of volatile substances of Streptomyces rochei A144 on apple tree rot at different growth stages (0, 10, 20, 25 and 30 d) was determined by plate coupling method, and the content of volatile substances was detected by headspace solid phase microextraction and gas chromatography-tandem mass spectrometry. After that, the volatile substances with good antibacterial activity under liquid and gas treatment were obtained by plate and coating method, respectively. 【Results】 When the growth time was 20 days, the inhibition rate of volatile substances produced by strain A144 was 100%. Strain A144 produced 63 volatile substances after 20 days of growth. When the concentration was 62.50 μL/L, the gas fumigation treatment of guaiacol, 1-octanol and dimethyl trisulfide could still completely inhibit the growth of apple tree rot. After diluting the original solution by 8 times, the liquid treatment of 1-octanol and dimethyl trisulfide with volume fraction of 12.5% could still completely inhibit the growth of apple tree rot. 【Conclusion】 The volatile substances 1-octanol and dimethyl trisulfide have the ability to significantly inhibit the pathogen of apple tree rot, and have the potential to be an important pesticide source of anti-apple tree rot.

Key words: Streptomyces rochei; growth time; volatile substances; Valsa mali

摘要：

【目的】 研究不同相态下娄彻氏链霉菌Streptomyces rochei A144挥发性物质对苹果树腐烂病菌壳囊孢Valsa mali的抑制效果,并进行体外检测,确定具有抑菌活性挥发性物质种类。【方法】 采用平板对扣法测定不同生长时期(0、10、20、25、30 d)娄彻氏链霉菌A144挥发性物质对苹果树腐烂病菌的抑制效果,使用顶空固相微萃取和气相色谱-串联质谱法检测分析其挥发性物质的含量,分别采用平板对扣法和涂抹法得出液态和气态处理下具有较好抑菌活性的挥发性物质。【结果】 当娄彻氏链霉菌A144生长至20 d时,菌株A144产生的挥发性物质对苹果树腐烂病菌的抑制率为100%;生长至20 d的菌株A144共产生了63种挥发性物质。当浓度为62.50 μL/L时,愈创木酚、1-辛醇和二甲基三硫气态熏蒸处理仍能完全抑制苹果树腐烂病菌的生长;将原液稀释8倍后,即体积分数为12.5% 的1-辛醇和二甲基三硫液态处理仍能完全抑制苹果树腐烂病菌的生长。【结论】 挥发性物质单体化合物1-辛醇和二甲基三硫具有显著抑制苹果树腐烂病菌的能力,有潜力作为抗苹果树腐烂病的重要开发农药药剂来源。

关键词: 娄彻氏链霉菌, 生长时期, 挥发性物质, 苹果树腐烂病菌

CLC Number:

S188

HUANG Wei, WANG Ning, SONG Bo, WANG Suling, QIN Xinzheng, SONG Suqin, LUO Yi, WEI Zengzhou, ZHANG Lijuan, WANG Wei. Inhibitory effect of different phases of Streptomyces rochei A144 volatile substances on Valsa mali var.mali[J]. Xinjiang Agricultural Sciences, 2024, 61(10): 2475-2483.

黄伟, 王宁, 宋博, 王苏玲, 秦新政, 宋素琴, 罗义, 魏增宙, 张丽娟, 王玮. 不同相态娄彻氏链霉菌A144挥发性物质对苹果树腐烂病菌的抑制效果[J]. 新疆农业科学, 2024, 61(10): 2475-2483.

Figures/Tables 6

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峰号 Peak no.	保留时间 Retention time(min)	相对含量 Relative content(%)	相似度 Similarity(%)	CAS号 CAS#	挥发性物质名称 Name of volaile substance
1	6.012	3.51	96	624-92-0	二甲基二硫/Disulfide, dimethyl
2	19.204	3	97	513-86-0	3-羟基-2-丁酮/Acetoin
3	21.582	4.23	97	3658-80-8	二甲基三硫/Dimethyl trisulfide
4	27.026	0.06	87	111-87-5	1-辛醇/1-Octanol
5	27.609	0.42	92	513-85-9	2,3-丁二醇/2,3-Butanediol
6	33.381	0.07	95	3796-70-1	香叶基丙酮/5,9-Undecadien-2-one, 6,10-dimethyl-, (E)-
7	33.57	0.75	98	90-5-1	愈创木酚/Phenol, 2-methoxy-
8	33.858	1.39	95	100-51-6	苯甲醇/Benzyl alcohol
9	34.634	53.94	97	60-12-8	苯乙醇/Phenylethyl Alcohol

峰号 Peak no.	保留时间 Retention time(min)	相对含量 Relative content(%)	相似度 Similarity(%)	CAS号 CAS#	挥发性物质名称 Name of volaile substance
1	6.012	3.51	96	624-92-0	二甲基二硫/Disulfide, dimethyl
2	19.204	3	97	513-86-0	3-羟基-2-丁酮/Acetoin
3	21.582	4.23	97	3658-80-8	二甲基三硫/Dimethyl trisulfide
4	27.026	0.06	87	111-87-5	1-辛醇/1-Octanol
5	27.609	0.42	92	513-85-9	2,3-丁二醇/2,3-Butanediol
6	33.381	0.07	95	3796-70-1	香叶基丙酮/5,9-Undecadien-2-one, 6,10-dimethyl-, (E)-
7	33.57	0.75	98	90-5-1	愈创木酚/Phenol, 2-methoxy-
8	33.858	1.39	95	100-51-6	苯甲醇/Benzyl alcohol
9	34.634	53.94	97	60-12-8	苯乙醇/Phenylethyl Alcohol

挥发性物质名称 Name of volatile substance	抑制率 Inhibition rate(%)
挥发性物质名称 Name of volatile substance	62.50 μL/L	125.00 μL/L	250.00 μL/L	500.00 μL/L
香叶基丙酮Geranyl acetone	0^e	22.97±4.64^d	67.20±1.17^c	73.31±1.95^b
苯甲醇Benzyl alcohol	70.38±1.08^b	82.29±0.79^b	80.10±1.87^b	100^a
3-羟基-2-丁酮3-hydroxy-2-butanone	3.23±0.97^d	13.08±0.71^e	12.48±0.99^f	12.66±3.58^c
愈创木酚Guaiacol	100^a	100^a	100^a	100^a
二甲基二硫Dimethyl disulfide	0^e	28.07±2.29^cd	45.54±2.17^e	100^a
辛醇1-octanol	100^a	100^a	100^a	100^a
二甲基三硫Dimethyl trisulfide	100^a	100^a	100^a	100^a
2,3-丁二醇2, 3-butanediol	0^e	0^f	0^g	0^d
苯乙醇Phenylethanol	35.29±3.54^c	34.53±3.56^c	51.83±1.46^d	71.74±1.16^b

挥发性物质名称 Name of volatile substance	抑制率 Inhibition rate(%)
挥发性物质名称 Name of volatile substance	62.50 μL/L	125.00 μL/L	250.00 μL/L	500.00 μL/L
香叶基丙酮Geranyl acetone	0^e	22.97±4.64^d	67.20±1.17^c	73.31±1.95^b
苯甲醇Benzyl alcohol	70.38±1.08^b	82.29±0.79^b	80.10±1.87^b	100^a
3-羟基-2-丁酮3-hydroxy-2-butanone	3.23±0.97^d	13.08±0.71^e	12.48±0.99^f	12.66±3.58^c
愈创木酚Guaiacol	100^a	100^a	100^a	100^a
二甲基二硫Dimethyl disulfide	0^e	28.07±2.29^cd	45.54±2.17^e	100^a
辛醇1-octanol	100^a	100^a	100^a	100^a
二甲基三硫Dimethyl trisulfide	100^a	100^a	100^a	100^a
2,3-丁二醇2, 3-butanediol	0^e	0^f	0^g	0^d
苯乙醇Phenylethanol	35.29±3.54^c	34.53±3.56^c	51.83±1.46^d	71.74±1.16^b

挥发性物质名称 Name of volatile substance	抑制率Inhibition rate(%)
挥发性物质名称 Name of volatile substance	原液 Original fluid	稀释2倍 Diluted 2 times	稀释4倍 Diluted 4 times	稀释8倍 Diluted 8 times
香叶基丙酮Geranyl acetone	100^a	80.23±0.66^b	75.91±0.53^b	70.51±1.25^c
苯甲醇Benzyl alcohol	100^a	100^a	100^a	26.76±4.29^e
3-羟基-2-丁酮3-hydroxy-2-butanone	0^b	0^c	0^c	0^f
愈创木酚Guaiacol	100^a	100^a	100^a	87.63±0.39^b
二甲基二硫Dimethyl disulfide	0^b	0^c	0^c	f
1-辛醇1-octanol	100^a	100^a	100^a	100^a
二甲基三硫Dimethyl trisulfide	100^a	100^a	100^a	100^a
2,3-丁二醇2, 3-butanediol	0^b	0^c	0^c	0^f
苯乙醇Phenylethanol	100^a	100^a	100^a	67.93±0.65^d

Inhibitory effect of different phases of Streptomyces rochei A144 volatile substances on Valsa mali var.mali

不同相态娄彻氏链霉菌A144挥发性物质对苹果树腐烂病菌的抑制效果

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