Effects of Adding Different Proportions of Cotton Stalks to the Diet on the Performance of Reserve Rams, Serum Related Hormones and Testicular Tissue

doi:10.6048/j.issn.1001-4330.2022.03.028

Abstract

Abstract:

【Objective】 The purpose of this experiment is to study the effects of adding different proportions of cotton stalks on the production performance, serum related hormones and testicular tissues of the reserve ram.【Method】 The experiment used a single factor completely randomized trial design, and randomly divided 60 small-tailed Han sheep baCKup breed of similar weight into 6 groups, 10 in each group.The diet of control group 1 (group CK₁) did not contain cotton source feed,and control group 2 (group CK₂) contained cotton seed meal and without cotton stalk.On the basis of CK₂ diet,the trial group diet was supplemented with 20% (group A),30% (group B),40% (group C) and 50% (group D) cotton stalks.The full duration of the trial was 70 days, including a preliminary trial period of 10 days and a formal trial period of 60 days.【Results】 (1)there were no significant differences in dry matter intake, daily gain and feed meat ratio between CK₁ and CK₂ groups (P>0.05); dry matter intake of group D was significantly higher than that of CK₂ group by 17.80% (P<0.01), extremely significantly higher than that of group A and C by 18.42% (P<0.01) and 16.58% (P<0.01), and significantly higher than that of group B by 4.65% (P<0.05); the daily gain and feed / meat ratio of group A, B, C, D and CK₂ were also different.There was no significant difference (P>0.05).(2) There was no significant difference in serum testosterone and gossypol between the experimental group and the control group (P> 0.05).(3) The growth and development of testes in the control group and the experimental group were normal without pathological injury.【Conclusion】 The addition of 20% -50% cotton stalks to diet does not affect the production performance of reserve ram, serum related hormones and testicular growth and development, and 20% cotton stalks are the best.

Key words: cotton straw; ram; testis; production performance; hormone level

摘要：

【目的】研究日粮中添加不同比例棉秆对后备种公羊生产性能、血清激素和睾丸组织的影响。【方法】采用单因素完全随机试验设计,将体重相近的60只小尾寒羊后备种公羊随机分为6组,每组10只。设计2个对照组,即饲粮中对照1组(CK₁组)不含棉源饲料,对照2组(CK₂组)含棉籽粕、不含棉秆。在CK₂组饲粮基础上,试验组饲粮分别添加20%(A组)、30%(B组)、40%(C组)和50%(D组)棉秆。全期70 d,其中预试期10 d,正试期60 d。【结果】CK₁组与CK₂组干物质采食量、日增重和料肉比差异不显著(P>0.05);D组干物质采食量极显著高于CK₂组17.80%(P<0.01),极显著高于A组和C组18.42%(P<0.01)和16.58%(P<0.01),显著高于B组4.65%(P<0.05);A、B、C、D组与CK₂组日增重和料肉比差异也不显著(P>0.05);对照组与及试验组血清睾酮、血清胰岛素和棉酚含量差异均不显著(P>0.05);对照组及试验组绵羊睾丸生长发育正常,未出现病理性伤害。【结论】日粮中添加20%~50%棉秆不影响后备种公羊生产性能、血清中相关激素和睾丸生长发育,添加20%棉秆时生长性能指标效果最好。

关键词: 棉秆, 种公羊, 睾丸, 生长性能, 激素水平

CLC Number:

S823

HOU Liangzhong, ZHANG Junyu, ZHANG Zhijun, SANG Duanji, GUO Tongjun, LI Congnian. Effects of Adding Different Proportions of Cotton Stalks to the Diet on the Performance of Reserve Rams, Serum Related Hormones and Testicular Tissue[J]. Xinjiang Agricultural Sciences, 2022, 59(3): 774-780.

侯良忠, 张俊瑜, 张志军, 桑断疾, 郭同军, 李聪年. 日粮中添加不同比例棉秆对后备种公羊生产性能、血清激素和睾丸组织的影响[J]. 新疆农业科学, 2022, 59(3): 774-780.

Figures/Tables 4

References 21

[1]	中华人民共和国国家统计局[EB/OL].https://data.stats.gov.cn/easyquery.htmcn=C01
	National Bureau of Statistics[EB/OL].https://data.stats.gov.cn/easyquery.htmcn=C01
[2]	苏玲玲, 郭同军, 张俊瑜, 等. 日粮添加不同比例棉秆对育肥绵羊生产性能及经济效益的影响[J]. 中国饲粮, 2020,(9): 122-125.
	SU Linlin, GUO Tongjun, ZHANG Junyu, et al. Effects of dietary supplementation of cotton stalk levels on Production performance and economic benefits of fattening sheep[J]. Chinese Feed, 2020,(9): 122-125.
[3]	古再丽努尔·艾麦提, 郭同军, 张俊瑜, 等. 日粮中不同水平棉秆对育肥期绵羊瘤胃发酵参数和屠宰性能的影响[J]. 新疆农业科学, 2020, 57(3): 581-588. DOI
	Guzalnur Amat, GUO Tongjun, ZHANG Junyu, et al. Effects of Different Levels of Cotton Stalk on Rumen Fermentation Parameters and Slaughter Performance of Fattening Sheep[J]. Xinjiang Agricultural Sciences, 2020, 57(3): 581-588. DOI
[4]	张俊瑜, 郭同军, 桑断疾, 等. 饲粮中不同水平棉秆对母羊生长性能和繁殖性能的影响[J]. 动物营养学报, 2020, 32(3): 1238-1246.
	ZHANG Junyu, GUO Tongjun, SANG Duanji, et al. Effects of Cotton Stalk Level in Diets on Growth Performance and Reproductive Performance of Ewe[J]. Chinese Journal of Animal Nutrition, 2018, 30(9): 3535-3542.
[5]	吐尔逊帕夏, 闻正顺, 潘晓亮, 等. 棉籽壳对雄性细毛羊生长性能及组织中棉酚残留的影响[J]. 浙江大学学报(农业与生命科学版), 2010, 36(3): 306-310.
	Tuerxun Paxia, WEN Zhengshun, PAN Xiaoliang, et al. Effect of cottonseed hull on growth performance and tissue residues of gossypol in fine-wool male sheep[J]. Journal of Zhejiang University (Agric.& Life Sci.), 2010, 36(3): 306-310
[6]	任广跃, 朱文学, 张仲欣, 等. 棉秆营养价值及其饲用模式分析[J]. 粮食与饲料业, 2009,(10): 28-30.
	REN Guangyue, ZHU Wenxue, ZHANG Zhongxin, et al. Analysis on nutritional value of cotton stalk and its feeding mode[J]. Food and Feed Industry, 2009,(10): 28-30.
[7]	张永建, 郝雷雷, 丁健, 等. 新疆棉秆发酵饲料加工利用[J]. 农业工程, 2014, 4(6): 44-46.
	ZHANG Yongjian, HAO Leilei, DIN Jian, et al. Processing and Utilization of Cotton Stalk Fermented Feed in Xinjiang[J]. Agricultural Engineering, 2014, 4(6): 44-46.
[8]	GB13078-2017. 饲料卫生标准[S].
	GB13078-2017. Hygienic Standard of Feed[S].
[9]	NY /T 816-2004肉羊饲养标准[S].
	NY /T 816—2004, Mutton sheep feeding standards[S].
[10]	郭同军, 臧长江, 王连群, 等. 去势对17~21月龄西门塔尔牛生长性能的影响[J]. 中国畜牧兽医, 2015, 42(5): 1173-1179.
	GUO Tongjun, ZANG Changjiang, WANG Lianqun, et al. The Influence of castration on GrowthPerformance of Simmental Cattle Aged from 17 to 21 Months[J]. Chinese Animal Husbandry and Veterinary Medicine, 2015, 42(5): 1173-1179.
[11]	李文娟, 刁其玉. 肉羊饲粮干物质采食量及其影响因素的研究进展[J]. 中国畜牧杂志, 2016, 32(19): 4-5.
	LI Wenjuan, DIAO Qiyu. Research progress of dry matter intake and its influencing factors in diet of mutton sheep[J]. China Journal of Animal Husbandry, 2016, 32(19): 4-5.
[12]	王文奇, 罗永明, 刘艳丰, 等. 不同棉秆水平全混合饲粮对绵羊生长性能和瘤胃发酵参数的影响[J]. 新疆农业科学, 2015, 52(11): 2111-2116.
	WANG Wenqi, LUO Yongming, LIU Yanfeng, et al. Effects of Diets with Different Cotton Stalk Levels of Total Mixed Rationon Growth Performance and Rumen Fermentation Parameters in Sheep[J]. Xinjiang Agricultural Science and Technology, 2015, 52(11): 2111-2116.
[13]	张俊瑜, 桑断疾, 张志军, 等. 饲粮中棉秆比例对绵羊生长性能和消化性能的影响[J]. 动物营养学报, 2018, 30(9): 3535-3542.
	ZHANG Junyu, SANG Duanji, ZHANG Zhijun, et al. Effects of Cotton Stalk Ratio in Diets on Growth and Digestive Performance of Sheep[J]. Chinese Journal of Animal Nutrition, 2018, 30(9): 3535-3542.
[14]	王镜岩, 朱圣庚, 徐长法. 生物化学[M]北京;高等教育出版社, 2002:550-589.
	WANG Jingyan, ZHU Shenggeng, XU Changfa. Biochemistry [M] Beijing: Higher Education Press, 2002, 550-589.
[15]	付华, 文继舫. 睾丸素的研究进展[J]. 国际病理科学与临床杂志, 2006,(4): 367-368.
	FU Hua, WEN Jifang. Research progress of testicle[J]. Journal of International Pathology and Clinical Medicine, 2006,(4): 367-368.
[16]	李朝晖, 刘彩娟, 伍翠娴, 等. 不同胰岛素用药方案治疗糖尿病酮症酸中毒的临床评价[J]. 岭南急诊医学杂志, 2020, 25(2): 170-171,190.
	LI Zhaohui, LIU Caijuan, WU Cuixian, et al. Clinical Evaluation of Different Insulin Drug Regimens in Treatment of Patients with Diabetes Ketoacidosis[J]. Lingnan Journal of Emergency Medicine, 2020, 25(2): 170-171,190.
[17]	崔钟秀. 胰岛素不同给药方式治疗妊娠糖尿病效果的临床比较[J]. 临床医药文献电子杂志, 2020, 7(14): 157.
	CUI Zhongxiu. Clinical comparison of the effects of different insulin administration methods on GesTational diabetes[J]. Electronic Journal of Clinical Medicine Literature, 2020, 7(14): 157.
[18]	张鹏, 杨自军, 宋超, 等. 高钼对小尾寒羊睾丸生精功能和病理损伤作用的研究[J]. 中国兽医学报, 2013, 33(5): 750-753,758.
	ZHANG Peng, YANG Zijun, SONG Chao, et al. Study on the spermatogenic function and pathological Damage of testis in Small Tail Han sheep by high molybdenum[J]. Chinese Journal of Veterinary Medicine, 2013, 33(5): 750-753,758.
[19]	王彤, 崔江波, 王芳, 等. 不同移植部位对成年大鼠睾丸间质细胞雄激素分泌功能的影响[J]. 现代生物医学进展, 2018, 18(13):
	WANG Tong, CUI Jiangbo, WANG Fang, et al. Effects of different transplantation sites on androgen secretion of adult rat Leydig cells[J]. Progress of Modern Biomedicine, 2018, 18(13):
[20]	潘思颖, 黄群, 覃天资, 等. 睾丸支持细胞相关激素影响的研究进展[J]. 右江民族医学院学报, 2018, 40(1): 82-86.
	PAN Siying, HUANG Qun, QIN Tianzi, et al. Research progress on the effects of testicular Sertoli cell related hormones[J]. Journal of Youjiang University of Ethnic Medicine, 2018, 40(1): 82-86.
[21]	陈思东, 许雅, 曾转萍. 醋酸棉酚对雄性小鼠生育能力影响的实验研究[J]. 广东药学院学报, 2007,(2): 172-174.
	CHEN Sidong, XU Ya, ZENG Zhuanping. A Study on the effects of gossypol acetate on fertility of male Mice[J]. Journal of Guangdong Pharmaceutical University, 2007,(2): 172-174.

项目 Items	试验处理 Experimental treatment
项目 Items	CK₁组 CK₁ group	CK₂组 CK₂ group	A组 A group	B组 B group	C组 C group	D组 D group
组成 Ingredients
棉秆 Cotton stalk	0.00	0.00	20.03	30.20	40.04	50.03
玉米秸秆 Corn stalk	22.95	22.09	10.20	6.23	2.83	0.00
苜蓿 Alfalfa	12.54	12.42	10.50	6.18	4.45	0.00
麦秸 Wheat straw	12.13	11.17	8.96	6.57	2.99	0.00
玉米 Corn	28.3	29.16	23.48	22.26	20.34	18.89
麸皮 Wheat bran	3.01	3.01	3.01	2.95	2.95	2.95
豆粕 Soybean meal	17.44	0.00	0.00	0.00	0.00	0.00
棉籽粕 Cotton seed meal	0.00	18.53	20.20	21.99	22.78	24.51
食盐 NaCl	0.80	0.80	0.80	0.80	0.80	0.80
碳酸氢钠 NaHCO₃	0.80	0.80	0.80	0.80	0.80	0.80
预混料Premix¹⁾	2.03	2.02	2.02	2.02	2.02	2.02
合计 Total	100.00	100.00	100.00	100.00	100.00	100.00
营养水平Nutrient levels²⁾
代谢能ME/(MJ/kg)	8.00	8.00	8.00	8.00	8.00	8.00
粗蛋白质 Cp	14.56	12.79	13.70	14.12	14.21	14.98
粗脂肪 EE	1.06	0.99	1.27	1.32	1.13	1.12
中性洗涤纤维 NDF	35.37	39.28	55.41	37.35	35.18	34.92
酸性洗涤纤维 ADF	19.67	23.50	41.14	26.57	27.87	27.61
钙 Ca	0.96	1.05	1.39	1.47	1.16	1.58
磷P	0.36	0.35	0.42	0.43	0.41	0.41
游离棉酚 Free gosspol/(mg/kg)	0	74.55	141.17	158.65	189.81	196.39

项目 Items	试验处理 Experimental treatment
项目 Items	CK₁组 CK₁ group	CK₂组 CK₂ group	A组 A group	B组 B group	C组 C group	D组 D group
组成 Ingredients
棉秆 Cotton stalk	0.00	0.00	20.03	30.20	40.04	50.03
玉米秸秆 Corn stalk	22.95	22.09	10.20	6.23	2.83	0.00
苜蓿 Alfalfa	12.54	12.42	10.50	6.18	4.45	0.00
麦秸 Wheat straw	12.13	11.17	8.96	6.57	2.99	0.00
玉米 Corn	28.3	29.16	23.48	22.26	20.34	18.89
麸皮 Wheat bran	3.01	3.01	3.01	2.95	2.95	2.95
豆粕 Soybean meal	17.44	0.00	0.00	0.00	0.00	0.00
棉籽粕 Cotton seed meal	0.00	18.53	20.20	21.99	22.78	24.51
食盐 NaCl	0.80	0.80	0.80	0.80	0.80	0.80
碳酸氢钠 NaHCO₃	0.80	0.80	0.80	0.80	0.80	0.80
预混料Premix¹⁾	2.03	2.02	2.02	2.02	2.02	2.02
合计 Total	100.00	100.00	100.00	100.00	100.00	100.00
营养水平Nutrient levels²⁾
代谢能ME/(MJ/kg)	8.00	8.00	8.00	8.00	8.00	8.00
粗蛋白质 Cp	14.56	12.79	13.70	14.12	14.21	14.98
粗脂肪 EE	1.06	0.99	1.27	1.32	1.13	1.12
中性洗涤纤维 NDF	35.37	39.28	55.41	37.35	35.18	34.92
酸性洗涤纤维 ADF	19.67	23.50	41.14	26.57	27.87	27.61
钙 Ca	0.96	1.05	1.39	1.47	1.16	1.58
磷P	0.36	0.35	0.42	0.43	0.41	0.41
游离棉酚 Free gosspol/(mg/kg)	0	74.55	141.17	158.65	189.81	196.39

项目 Items	CK₁组 CK₁ group	CK₂组 CK₂ group	A组 A group	B组 B group	C组 C group	D组 D group	SEM	P值 P value
干物质采食量 Dry matter intake (kg/d)	1.93^Bb	1.91^Bb	1.90^Bb	2.15^ABb	1.93^Bb	2.25^Aa	0.072	0.01
日增重 Daily gaing (g/d)	224.72	191.67	179.58	175.42	146.67	141.04	32.322	0.38
料肉比 Feed conversion ratio	8.72	9.97	11.33	13.21	13.54	18.78	3.581	0.31

项目 Items	CK₁组 CK₁ group	CK₂组 CK₂ group	A组 A group	B组 B group	C组 C group	D组 D group	SEM	P值 P value
干物质采食量 Dry matter intake (kg/d)	1.93^Bb	1.91^Bb	1.90^Bb	2.15^ABb	1.93^Bb	2.25^Aa	0.072	0.01
日增重 Daily gaing (g/d)	224.72	191.67	179.58	175.42	146.67	141.04	32.322	0.38
料肉比 Feed conversion ratio	8.72	9.97	11.33	13.21	13.54	18.78	3.581	0.31

项目 Items	CK₁组 CK₁ group	CK₂组 CK₂ group	A组 A group	B组 B group	C组 C group	D组 D group	SEM	P值 P value
睾酮 Testosteronen (ng/dL)	127.02	116.85	78.56	72.88	66.13	64.91	46.993	0.82
胰岛素 InsulinnμIU/(mL)	11.16	12.28	12.16	17.43	8.42	15.01	3.437	0.38
游离棉酚 Free gosspolμg/(mL)	ND	0.10	0.21	0.30	0.19	0.33	0.056	0.51