Effects of PEG-6000 and NaCl Stress on Seed Germination  and Seedling Growth of the Tobacco with GbWRKY32 Gene

doi:10.6048/j.issn.1001-4330.2018.08.006

Abstract

Abstract: 【Objective】 To study the drought resistance and salt tolerance of GbWRKY32 gene.【Method】 GbWRKY32 transgenic tobacco and wild type tobacco were used as materials to study the effects of different concentrations of PEG-6000 (0%, 5%, 10% and 15%) and NaCl (0 mm, 100 mm, 150 mm and 200 mm) on seed germination, seedling length, root system, dry weight and fresh weight of GbWRKY32 transgenic tobacco.【Result】 (1) With the increase of PEG-6000 concentration, the seed germination rate of GbWRKY32 transgenic tobacco seeds and native tobacco seeds decreased, and the root weight, dry weight and fresh weight decreased; (2) After treatment of 15% PEG-6000 for 14 days, the seed germination rate, seedling length, root system, dry weight and fresh weight of the three transgenic tobacco lines GbWRKY32 were significantly higher than those of wild type tobacco seeds; (3) With the increase of NaCl concentration, the whole trend of NaCl was the same as that of PEG stress. After 14 days of treatment, the seed germination rate, seedling length, root system, dry weight and fresh weight of three transgenic tobacco lines with GbWRKY32 gene were significantly higher than those of wild type tobacco seeds. 【Conclusion】Through seed germination rate, root scanning and fresh weight and dry weight measurement, it was found that GbWRKY32 gene transferred into tobacco could significantly improve the resistance of tobacco to drought and salt stress.

Key words: transgenic tobacco; abiotic stress; germination rate; root scanning; amount of substance

摘要： 【目的】研究GbWRKY32基因的抗旱及耐盐功能。【方法】以转GbWRKY32基因烟草以及本生烟草为材料,研究在不同浓度PEG-6000(0%、5%、10%、15%)以及不同浓度NaCl(0 mm、100 mm、150 mm、200 mm)处理下,对转GbWRKY32基因烟草种子萌发、全苗长、根系以及干重、鲜重的影响。【结果】(1)随着PEG-6000浓度的不断增加转GbWRKY32基因烟草种子与本生烟草种子萌发率全苗长、根系以及干重、鲜重不断降低;(2)在PEG-6000浓度达到15%处理14 d后,三个转GbWRKY32基因烟草株系的种子萌发率、全苗长、根系以及干重、鲜重明显高于本生烟草种子;(3)随着NaCl浓度的不断上升,其整体趋势与PEG胁迫相同;在NaCl浓度达到150 mm时,处理14 d以后三个转GbWRKY32基因烟草株系的种子萌发率、全苗长、根系以及干重、鲜重明显高于本生烟草种子;【结论】GbWRKY32基因转入烟草中可以明显提高烟草对干旱以及盐胁迫的抵御能力。

关键词: 转基因烟草, 非生物胁迫, 萌发率, 根系扫描, 物质的量

CLC Number:

S188

GUO Ding, QU Yang-ying, NI Zhi-yong, CHEN Quan-jia. Effects of PEG-6000 and NaCl Stress on Seed Germination and Seedling Growth of the Tobacco with GbWRKY32 Gene[J]. Xinjiang Agricultural Sciences, 2018, 55(8): 1417-1427.

郭丁,曲延英,倪志勇,陈全家. PEG-6000和NaCl胁迫对转GbWRKY32基因烟草种子萌发及苗期生长的影响[J]. 新疆农业科学, 2018, 55(8): 1417-1427.

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Effects of PEG-6000 and NaCl Stress on Seed Germination and Seedling Growth of the Tobacco with GbWRKY32 Gene

PEG-6000和NaCl胁迫对转GbWRKY32基因烟草种子萌发及苗期生长的影响

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