Effects of PEG-6000 and NaCl Stress on Seed Germination  and Seedling Growth of the Tobacco with GbWRKY32 Gene

doi:10.6048/j.issn.1001-4330.2018.08.006

Abstract

Abstract: 【Objective】 To study the drought resistance and salt tolerance of GbWRKY32 gene.【Method】 GbWRKY32 transgenic tobacco and wild type tobacco were used as materials to study the effects of different concentrations of PEG-6000 (0%, 5%, 10% and 15%) and NaCl (0 mm, 100 mm, 150 mm and 200 mm) on seed germination, seedling length, root system, dry weight and fresh weight of GbWRKY32 transgenic tobacco.【Result】 (1) With the increase of PEG-6000 concentration, the seed germination rate of GbWRKY32 transgenic tobacco seeds and native tobacco seeds decreased, and the root weight, dry weight and fresh weight decreased; (2) After treatment of 15% PEG-6000 for 14 days, the seed germination rate, seedling length, root system, dry weight and fresh weight of the three transgenic tobacco lines GbWRKY32 were significantly higher than those of wild type tobacco seeds; (3) With the increase of NaCl concentration, the whole trend of NaCl was the same as that of PEG stress. After 14 days of treatment, the seed germination rate, seedling length, root system, dry weight and fresh weight of three transgenic tobacco lines with GbWRKY32 gene were significantly higher than those of wild type tobacco seeds. 【Conclusion】Through seed germination rate, root scanning and fresh weight and dry weight measurement, it was found that GbWRKY32 gene transferred into tobacco could significantly improve the resistance of tobacco to drought and salt stress.

Key words: transgenic tobacco; abiotic stress; germination rate; root scanning; amount of substance

摘要： 【目的】研究GbWRKY32基因的抗旱及耐盐功能。【方法】以转GbWRKY32基因烟草以及本生烟草为材料,研究在不同浓度PEG-6000(0%、5%、10%、15%)以及不同浓度NaCl(0 mm、100 mm、150 mm、200 mm)处理下,对转GbWRKY32基因烟草种子萌发、全苗长、根系以及干重、鲜重的影响。【结果】(1)随着PEG-6000浓度的不断增加转GbWRKY32基因烟草种子与本生烟草种子萌发率全苗长、根系以及干重、鲜重不断降低;(2)在PEG-6000浓度达到15%处理14 d后,三个转GbWRKY32基因烟草株系的种子萌发率、全苗长、根系以及干重、鲜重明显高于本生烟草种子;(3)随着NaCl浓度的不断上升,其整体趋势与PEG胁迫相同;在NaCl浓度达到150 mm时,处理14 d以后三个转GbWRKY32基因烟草株系的种子萌发率、全苗长、根系以及干重、鲜重明显高于本生烟草种子;【结论】GbWRKY32基因转入烟草中可以明显提高烟草对干旱以及盐胁迫的抵御能力。

关键词: 转基因烟草, 非生物胁迫, 萌发率, 根系扫描, 物质的量

CLC Number:

S188

GUO Ding, QU Yang-ying, NI Zhi-yong, CHEN Quan-jia. Effects of PEG-6000 and NaCl Stress on Seed Germination and Seedling Growth of the Tobacco with GbWRKY32 Gene[J]. Xinjiang Agricultural Sciences, 2018, 55(8): 1417-1427.

郭丁,曲延英,倪志勇,陈全家. PEG-6000和NaCl胁迫对转GbWRKY32基因烟草种子萌发及苗期生长的影响[J]. 新疆农业科学, 2018, 55(8): 1417-1427.

References

[1] Vinocur, B., & Altman, A. (2005). Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Current Opinion in Biotechnology, 16(2): 123-132.
[2]王海标.棉花品种苗期与花期抗旱性评价及遗传多样性分析[D].乌鲁木齐:新疆农业大学硕士论文,2013.
WANG Hai-biao. (2016). Drought Resistance Evaluation and genetic Diversity Analysis of Cotton varieties at Seedling and flowering stages [D]. Master Dissertation. Xinjiang Agricultural University, Urumqi, (in Chinese)
[3] Cattivelli, L., Baldi, P., Crosatti, C., Fonzo, N. D., Faccioli, P., & Grossi, M., et al. (2002). Chromosome regions and stress-related sequences involved in resistance to abiotic stress in triticeae. Plant Molecular Biology, 48(5-6): 649-665.
[4]郭瑞霞.管晓丹.张艳婷.我国荒漠化主要研究进展[J].干旱气象,2015,33(3):505-514.
GUO Rui-xia, GUAN Xiao-dan, ZHANG Yan-ting. (2015). Main Advances in Desertification Research in China [J]. Journal of Arid Meteorology , 33(3): 505-514. (in Chinese)
[5]岳惠玲.盐碱胁迫下烟草出芽率和脯氨酸·丙二醛活性变化[J].安徽农业科学,2011,39(18):10 804-10 806.
YOU Hui-ling. (2011). Changes of Sprouting Rate of Tobacco, Proline and MDA Activity in Saline Environment [J]. Journal of Arid Meteorology , 39(18): 10,804-10,806. (in Chinese)
[6]唐益苗,赵昌平,高世庆,等.植物抗旱相关基因研究进展[J].麦类作物学报,2009,29(1):166-173.
TANG Yi-miao, ZHAO Chang-ping, GAO Shi-qing, et al. (2009). Advances in Genes Related to Plant Drought Tolerance [J]. Journal of Arid Meteorology , 29(1): 166-173. (in Chinese)
[7] Ulker, B., & Somssich, I. E. (2004). Wrky transcription factors: from dna binding towards biological function. Current Opinion in Plant Biology, 7(5): 491-498.
[8]李冉,娄永根.植物中逆境反应相关的WRKY转录因子研究进展[J].生态学报,2011, 31(11): 3 223-3 231.
Li Wei, Yan Yonggen.Advances in research on WRKY transcription factors related to stress in plants[J].Journal of Ecology,2011, 31 (11) : 3,223-3,231.
[9] Zhou, Q. Y., Tian, A. G., Zou, H. F., Xie, Z. M., Lei, G., & Huang, J., et al. (2008). Soybean wrky-type transcription factor genes, gmwrky13, gmwrky21, and gmwrky54, confer differential tolerance to abiotic stresses in transgenic arabidopsis plants. Plant Biotechnology Journal,6(5): 486-503.
[10] Devaiah, B. N., Karthikeyan, A. S., & Raghothama, K. G. (2007). Wrky75 transcription factor is a modulator of phosphate acquisition and root development in arabidopsis. Plant Physiology, 143(4): 1,789-1,801.
[11] Zhang, J., Peng, Y., & Guo, Z. (2008). Constitutive expression of pathogen-inducible oswrky31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants. Cell research,18(4): 508-521.
[12]R Robatzek, S., & Somssich, I. E. (2001). A new member of the arabidopsis wrky transcription factor family, atwrky6, is associated with both senescence‐ and defence‐related processes. Plant Journal,28(2): 123-133.
[13] lker, B., Mukhtar, M. S., & Somssich, I. E. (2007). The wrky70 transcription factor of arabidopsis, influences both the plant senescence and defense signaling pathways. Planta, 226(1):125-137.
[14] Jing, S., Zhou, X., Song, Y., & Yu, D. (2009). Heterologous expression of oswrky23, gene enhances pathogen defense and dark-induced leaf senescence in arabidopsis. Plant Growth Regulation, 58(2): 181-190.
[15] Johnson, C. S., Kolevski, B., & Smyth, D. R. (2002). Transparent testa glabra2, a trichome and seed coat development gene of arabidopsis, encodes a wrky transcription factor. Plant Cell, 14(6):1,359-1,375.
[16] Sun, C., Palmqvist, S., Olsson, H., Borén, M., Ahlandsberg, S., & Jansson, C. (2003). A novel wrky transcription factor, susiba2, participates in sugar signaling in barley by binding to the sugar-responsive elements of the iso1 promoter. Plant Cell, 15(9): 2,076-2,092.
[17] Xu, Y. H., Wang, J. W., Wang, S., Wang, J. Y., & Chen, X. Y. (2004). Characterization of gawrky1, a cotton transcription factor that regulates the sesquiterpene synthase gene (+)-delta-cadinene synthase-a. Plant Physiology, 135(1): 507-515.
[18] Zhang, Z. L., Xie, Z., Zou, X., Casaretto, J., Ho, T. D., & Shen, Q. J. (2004). A rice wrky gene encodes a transcriptional repressor of the gibberellin signaling pathway in aleurone cells. Plant Physiology, 134(4): 1,500-1,513.
[19] Xie, Z., Zhang, Z. L., Zou, X., Yang, G., Komatsu, S., & Shen, Q. J. (2006). Interactions of two abscisic-acid induced wrky genes in repressing gibberellin signaling in aleurone cells. Plant Journal, 46(2): 231-242.
[20] Xie, Z., Zhang, Z. L., Hanzlik, S., Cook, E., & Shen, Q. J. (2007). Salicylic acid inhibits gibberellin-induced alpha-amylase expression and seed germination via a pathway involving an abscisic-acid-inducible wrky, gene. Plant Molecular Biology, 64(3): 293-303.
[21] Zou, X., Neuman, D., & Shen, Q. J. (2008). Interactions of two transcriptional repressors and two transcriptional activators in modulating gibberellin signaling in aleurone cells. Plant Physiology, 148(1): 176-186.
[22] Wu, X., Shiroto, Y., Kishitani, S., Ito, Y., & Toriyama, K. (2009). Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing oswrky11, under the control of hsp101, promoter. Plant Cell Reports, 28(1): 21-30.
[23] Wang, Z., Zhu, Y., Wang, L., Liu, X., Liu, Y., & Phillips, J., et al. (2009). A wrky transcription factor participates in dehydration tolerance in boea hygrometrica by binding to the w-box elements of the galactinol synthase (bhgols1) promoter. Planta, 230(6): 1,155-1,166.
[24] Niu, C., Wei, W., Zhou, Q., Tian, A., Hao, Y., & Zhang, W., et al. (2012). Wheat wrky genes tawrky2 and tawrky19 regulate abiotic stress tolerance in transgenic arabidopsis plants. Plant Cell & Environment,35(6): 1,156-1,170.
[25]加得拉·吐留汗.海岛棉GbWRKY32、GbWRKY40和GbHCT基因的克隆[D].乌鲁木齐:新疆农业大学学报硕士论文,2016.
Jiadela Duliuhan. (2016). Cloning of GbWRKY32,GbWRKY40 and GbHCT genes from Sea Island cotton [D]. Master Dissertation. Xinjiang Agricultural University, Urumqi, (in Chinese)
[26] Zhang, S., Hu, J., Zhang, Y., Xie, X. J., & Knapp, A. (2007). Seed priming with brassinolide improves lucerne (medicago sativa l.) seed germination and seedling growth in relation to physiological changes under salinity stress. Australian Journal of Agricultural Research, 58(8): 811-815.
[27]刘玲玲,丁永乐,程传策,等.4个烟草品种干旱胁迫下萌发和苗期生理特性及抗旱性评价[J].江苏农业科学,2015, (5) :104-107.
LIU Ling-ling, DING Yong-le, CHEN Chuan-ce, et al. (2015). Physiological characteristics and drought resistance evaluation of four tobacco varieties under drought stress during germination and seedling stage [J]. Jiangsu Agricultural Sciences, (5):104-107. (in Chinese)
[28]许灵杰,杜相革,郑登峰,等.模拟干旱胁迫对不同烤烟品种萌发和幼苗生长的影响[J].贵州农业科学,2014, (9) :48-51.
XU Ling-jie, DU Xiang-ge, ZGENG Deng-feng, et al. (2014). Effects of Simulated Drought Stress on Germination and Seedling Growth of Different Flue-cured Tobacco Varieties [J]. Guizhou Agricultural Sciences, (9):48-51. (in Chinese)
[29]彭耀东,徐庆凯,钟玉德,等.聚乙二醇(PEG)溶液浸种对烟草种子发芽整齐度的影响研究[J].园艺与种苗,2014,(2):1-4.
PENG Yao-dong, XU Qin-kai, ZHONG Yu-de, et al. (2014). Study on Effects of PEG on Germinating Uniformity of Tobacco Seeds [J]. Horticulture and Seed, (2):1-4. (in Chinese)
[30]马文广, 崔华威, 李永平,等. 20个烟草品种干旱胁迫下发芽和苗期生理特性及耐旱性评价[J].种子,2012,(2):25-30.
MA Wen-guan, CUI Hua-wei, LI Yong-ping, et al. (2012). Physiological Characteristics of 20 Tobacco Cultivars under Drought Stress and Assessment of Their Drought Tolerance at Germination and Seedling Stages [J]. Seed, (2):25-30. (in Chinese)
[31] Jiang, Y., & Deyholos, M. K. (2009). Functional characterization of arabidopsis nacl-inducible wrky25 and wrky33 transcription factors in abiotic stresses. Plant Molecular Biology, 69(1-2): 91-105.

Effects of PEG-6000 and NaCl Stress on Seed Germination and Seedling Growth of the Tobacco with GbWRKY32 Gene

PEG-6000和NaCl胁迫对转GbWRKY32基因烟草种子萌发及苗期生长的影响

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