Effects of planting pattern on the diversity of fungi in Isatidis Radix

doi:10.6048/j.issn.1001-4330.2025.02.018

Xinjiang Agricultural Sciences ›› 2025, Vol. 62 ›› Issue (2): 412-418.DOI: 10.6048/j.issn.1001-4330.2025.02.018

• Plant Protection·Microbes • Previous Articles Next Articles

Effects of planting pattern on the diversity of fungi in Isatidis Radix

CHEN Yong¹(), SONG Suqin², LIN Caixia³(), QIAO Xu⁴()

1. Institute of Soil, Fertilizer and Agricultural Water Conservation, Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China
2. Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs / Xinjiang Key Laboratory of Agricultural Bio-safety, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China
3. Agricultural Technology Extension Station of Xinjiang Uygur Autonomous Region, Urumqi 830011,China
4. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine / Ministry of Education National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China

Received:2024-08-15 Online:2025-02-20 Published:2025-04-17
Correspondence author: LIN Caixia, QIAO Xu
Supported by:
Key Research and Development Program of Xinjiang Uygur Autonomous Region,key special project of Department Local Cooperation "Research and demonstration of beeding and green intelligent quality control common key Technologies for Xinjing's advantageous and characteristic medicinal plant varieties"(2024B040039);Key Research and Development Program of Xinjiang Uygh Autonomous Region,key special project of Department Local Cooperation "Research and development of key technologies for high-quality three-dimensional planting of seabuckthorn and chinese herbal medicine" subtopics"Resarch and pemonstration on optimal selection of seabuckthorn Chinese" herbal three dimensional planting varieties(2023B02007-1)

种植模式对板蓝根内生真菌多样性的影响

陈勇¹(), 宋素琴², 蔺彩霞³(), 乔旭⁴()

1.新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830091
2.新疆农业科学院植物保护研究所/农业农村部西北荒漠作物有害生物综合治理重点实验室/新疆农业生物安全重点实验室,乌鲁木齐 830091
3.新疆维吾尔自治区农业技术推广总站,乌鲁木齐 830011
4.中国医学科学院北京协和医学院药用植物研究所/中草药物质基础与资源利用教育部重点实验室 / 濒危药材繁育国家工程实验室,北京 100193

通讯作者: 蔺彩霞,乔旭
作者简介:陈勇(1977- ),男,安徽六安人,副研究员,硕士研究生,研究方向为药用植物品种选育及栽培,(E-mail)chenyong@xaas.ac.cn
基金资助:
新疆维吾尔自治区重点研发计划“厅地联动”重点专项“新疆优势特色中药材品种选育及绿色智能质控共性关键技术研究与示范”(2024B040039);新疆维吾尔自治区重点研发计划“厅地联动”重点专项“沙棘-中草药立体高质高效种植关键技术研发”子课题“沙棘-中草药立体种植品种优选研究及示范”(2023B02007-1)

Abstract

Abstract:

【Objective】Endophytic fungi have a significant impact on the physiology, development and health of the host, can stimulate the germination and growth of the host plant, and play an important role in regulating the nutrient absorption and enhancing the stress resistance of the host plant. This study aims to reveal the effects of different planting patterns of one-year spring planting (SPS1), two-year spring continuous planting (SPS2) and one-year summer planting (SUS) on the fungal community structure of Isatidis root in Xinjiang. 【Methods】High throughput sequencing technology was used to identify and analyze endophytic fungi community structure of Radix isatidis root. 【Results】At the phylum level, endorhizoidal fungi were mainly composed of Ascomycota, Mortierellomycota, Basidiomycota, Chytridiomycota, Olpidiomycota,Blastocladiomycota, Glomeromycota, Rozellomycota and Mucoromycota. Compared with SUS, SPS1 decreased the relative abundance of Neonectria and Penicillium significantly, but increased the relative abundance of Oedocephalum. SPS2 increased the abundences of Ascomycota and Chytridiomycota significantly, but significantly decreased the abundences of Mortierellomycota. However, it increased the relative abundance of Fusarium, Aspergillus, Acremonium, Gibberella and Bisifusarium. Compared with SPS1, SPS2 decreased the relative abundations of Mortierella and Plectosphaerella significantly, but increased the relative abundances of Fusarium, Acremonium and Neonectria. 【Conclusion】The population structure of endophytic fungi in isatidis root is different under different planting patterns. Continuous planting would decrease the abundance of beneficial fungi and increase the relative abundance of pathogenic fungi and increase the risk of continuous cropping obstacles.

Key words: Isatidis Radix; different planting patterns; high-throughput sequencing; endophytic fungus diversity; community composition

摘要：

【目的】研究不同种植模式下,新疆1年春季种植 (SPS1)、2年春季连作种植(SPS2)以及一年夏季种植(SUS)板蓝根对板蓝根根内真菌群落结构的影响。【方法】利用高通量测序技术对板蓝根根系内生真菌进行鉴定与分析根系内生真菌群落结构。【结果】根内真菌在门水平上主要由子囊菌门Ascomycota,被孢霉门 Mortierellomycota,担子菌门Basidiomycota,壶菌门Chytridiomycota,油壶菌门Olpidiomycota,芽枝霉门Blastocladiomycota,球囊菌门 Glomeromycota,罗兹菌门Rozellomycota和毛霉门Mucoromycota组成。与SUS相比,SPS1显著降低了杓兰菌根菌Neonectria以及青霉菌Penicillium的相对丰度,但是却提高了头珠霉属Oedocephalum的相对丰度, SPS2显著增加了土壤Ascomycota和Chytridiomycota的丰度,显著降低了Mortierellomycota的丰度;但提高了镰刀菌Fusarium,黄曲霉 Aspergillus,枝顶孢霉属Acremonium,赤霉属Gibberella以及新月双镰孢Bisifusarium物种的相对丰度。与SPS1相比,SPS2显著降低了Mortierella和 Plectosphaerella的相对丰度,但提高了镰刀菌Fusarium,枝顶孢霉属Acremonium和杓兰菌根菌Neonectria的相对丰度。【结论】不同种植模式下板蓝根内生真菌的种群结构存在差异,连续种植会降低有益真菌丰度和增加病原真菌的相对丰度,增加连作障碍的风险。

关键词: 板蓝根, 不同种植模式, 高通量测序, 内生真菌多样性, 群落组成

CLC Number:

S567

CHEN Yong, SONG Suqin, LIN Caixia, QIAO Xu. Effects of planting pattern on the diversity of fungi in Isatidis Radix[J]. Xinjiang Agricultural Sciences, 2025, 62(2): 412-418.

陈勇, 宋素琴, 蔺彩霞, 乔旭. 种植模式对板蓝根内生真菌多样性的影响[J]. 新疆农业科学, 2025, 62(2): 412-418.

Figures/Tables 6

Tab.1 Changes of different cropping system on soil parameters

	SUS	SPS	SPS2
pH	8.73 ± 0.19^a	8.41 ± 0.088^b	8.87 ± 0.034^a
SOC	13.95 ± 0.450 92^a	15.30 ± 2.57^a	16.28 ± 0.88^a
Nmin	51.38 ± 3.50^b	58.83 ± 5.75^a	62.53 ± 4.22^a
AP	14.31 ± 2.89^b	22.40 ± 9.00^b	33.93 ± 3.88^a
AK	392.00 ± 46.90^ab	462.00 ± 85.70^a	312.50 ± 32.73^b
TN	0.81 ± 0.05^c	0.92 ± 0.08^b	0.96 ± 0.06^a
TP	1.03 ± 0.48^a	1.02 ± 0.54^a	0.63 ± 0.19^a
TK	16.45 ± 5.28^a	13.74 ± 0.85^a	14.57 ± 3.94^a

Fig.1 Changes of different cropping system on microbial diversity and community

Fig.2 Root microbial composition in different cropping system

Fig.3 Changes of differential species in different cropping system

Fig 4 Changes of different cropping system on microbial network and network parameters

Fig.5 Changes of different soil parameters on microbial community

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Effects of planting pattern on the diversity of fungi in Isatidis Radix

种植模式对板蓝根内生真菌多样性的影响

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