Study on Electroantennogram and Behavioral Response of Potosia brevitarsis Lewis to 9 Volatiles in Sugar-Vinegar Liquid

doi:10.6048/j.issn.1001-4330.2022.02.025

Abstract

Abstract:

【Objective】 To elucidate the reason why Potosia brevitarsis Lewis is attracted to sugar-vinegar liquid, and to search for compounds with attractant activity to Potosia brevitarsis Lewis. 【Method】 EAG and “Y”-typed olfactometer were used to tested the EAG and behavioral response of Potosia brevitarsis Lewis. 【Result】 Potosia brevitarsis Lewis contained EAG reaction to 9 volatiles of sugar-vinegar liquid, and the female and male adults had higher EAG reaction values to ethyl valerate, 2, 2, 4, 6, 6-pentamethyl-heptane and furfural, the male adults also had higher EAG reaction values to ethyl lactate and hexanoic acid.The analysis of “Y”-typed olfactometer showed that 2, 2, 4, 6, 6- pentamethyl-heptane and ethyl lactate had good attraction to both male and female adults of the Potosia brevitarsis Lewis.There were gender differences in the selection of furfural and hexanoic acid between female and male adults.Furfural had a good attraction to female adults, but male adults had no obvious tendency to them and hexanoic acid had a good attraction to male adults, while female adults had no obvious tropism to them. 【Conclusion】 2,2,4,6,6-pentamethyl-heptane, ethyl lactate, furfural and hexanoic acid can be used as the components of the attractant formula for the Potosia brevitarsis Lewis.

Key words: Potosia brevitarsis Lewis; sugar-vinegar liquid; volatile; electroantennogram (EAG); “Y”-typed olfactometer

摘要：

【目的】 研究白星花金龟(Protaetia brevitarsis Lewis)对糖醋液的趋性,寻找对白星花金龟具有引诱活性的化合物。【方法】 运用触角电位(electroantennogram,EAG)仪和“Y”型嗅觉仪测定白星花金龟触角电位和行为反应。【结果】 白星花金龟对9种糖醋液挥发物均有一定的EAG反应,雌、雄成虫对戊酸乙酯、2,2,4,6,6-五甲基庚烷和糠醛有较高的EAG反应值,此外,雄成虫还对乳酸乙酯和正己酸有较高的EAG反应值。“Y”型嗅觉仪分析表明,2,2,4,6,6-五甲基庚烷和乳酸乙酯对白星花金龟雌、雄两性成虫均有良好的引诱作用;白星花金龟雌、雄成虫对糠醛和正己酸的选择存在性别差异,糠醛对雌成虫具有较好引诱作用,雄成虫对其无明显趋性,正己酸对雄成虫具有较好引诱作用,雌成虫对其无明显趋性。【结论】 2,2,4,6,6-五甲基庚烷、乳酸乙酯、糠醛和正己酸可作为白星花金龟引诱剂配方的组成成分。

关键词: 白星花金龟, 糖醋液, 挥发物, EAG, “Y”型嗅觉仪

CLC Number:

LI Mingjie, WANG Shaoshan. Study on Electroantennogram and Behavioral Response of Potosia brevitarsis Lewis to 9 Volatiles in Sugar-Vinegar Liquid[J]. Xinjiang Agricultural Sciences, 2022, 59(2): 466-473.

李明杰, 王少山. 白星花金龟对糖醋液9种挥发物的触角电位和行为反应[J]. 新疆农业科学, 2022, 59(2): 466-473.

Figures/Tables 4

Table 1 Standard compounds used to determine the Electroantennogram response of the Potosia brevitarsis Lewis

序号 Code	化合物 Compound	CAS号 CAS number	纯度 Purity (%)	来源 Source
1	戊酸乙酯	539-82-2	≥99.5	Macklin
2	糠醛	98-01-1	≥99.5	Macklin
3	2,2,4,6,6-五甲基庚烷	13475-82-6	≥98	Macklin
4	L-乳酸乙酯	687-47-8	≥98	Sigma
5	乳酸乙酯	97-64-3	≥98	Sigma
6	己酸乙酯	123-66-0	≥99	Sigma
7	丁酸乙酯	105-54-4	≥99.5	Macklin
8	正己酸	142-62-1	≥98	Sigma
9	2,3-丁二醇	513-85-9	≥98	Macklin

Fig.1 Electroantennographic responses of female and male adults of the Potosia brevitarsis Lewis to different volatiles Note:The small letters (capital letters) indicate significant differences between female and male adults at the P<0.05(P<0.01) levels; the numbers indicate the relative EAG values of the volatiles; **indicates significant difference in the EAG response between female and male adults to the same volatile (P< 0.01) (T-test)

Fig.2 Behavioral selective responses of female (A) and male (B) adults of Potosia brevitarsis Lewis to different volatiles Note: *、** indicate significant difference between the treatment and the control P<0.05 or P<0.01 level by χ2 test, respectively; ns indicate no significant difference in the results of the χ2 test

Table 2 Behavioral selective response rate of female and male adults of Potosia brevitarsis Lewis to different volatiles

挥发物 Volatiles	性别 Sex	反应率 Response rate(%)	选择反应率 Selection response rate(%)	选择系数 Selection coefficient
乳酸乙酯 Ethyl lactate	雌虫	100.00	65.00	0.30
乳酸乙酯 Ethyl lactate	雄虫	100.00	66.67	0.33
正己酸 Hexanoic acid	雌虫	95.00	54.39	0.09
正己酸 Hexanoic acid	雄虫	98.33	64.41	0.29
2,2,4,6,6-五甲基庚烷 2,2,4,6,6-pentamethyl-Heptane	雌虫	95.00	63.16	0.26
2,2,4,6,6-五甲基庚烷 2,2,4,6,6-pentamethyl-Heptane	雄虫	93.33	64.29	0.29
糠醛 Furfural	雌虫	96.67	65.52	0.31
糠醛 Furfural	雄虫	100.00	48.33	-0.03
戊酸乙酯 Ethyl valerate	雌虫	100.00	55.00	0.10
戊酸乙酯 Ethyl valerate	雄虫	91.67	45.46	-0.09

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