Identification of Active Components in Feces of Monolepta hieroglyphica (Motschulsky)

doi:10.6048/j.issn.1001-4330.2020.06.014

Abstract

Abstract: 【Objective】 To screen out the compounds with electrophysiological and behavioral activities from the fecal volatile compounds of Monolepta hieroglyphica (Motschulsky) adults.【Method】 The components and contents of the volatile compounds in the feces were collected and identified by using GC-MS. The electrophysiological and behavioral responses of the female and male adults of the beetle to four kinds of volatile compounds in the feces were measured by the EAG and "Y" olfactometer. 【Result】There were 29 compounds in the feces of the adult beetle, including alkenes, aldehydes, esters, acids, alcohols, etc. The EAG responses of the female and male adults to α-caryophyllene, α-pinene, Dragosantol were obvious, and the male and female insects had obvious convergence to 10 μL/mL Dragosantol and α-pinene. 【Conclusion】Dragosantol and α-pinene may be the active components in the feces of the adult.

Key words: Monolepta hieroglyphica; fecal volatiles; EAG response; behavioral response; active component

摘要： 【目的】研究从双斑长跗萤叶甲Monolepta hieroglyphica (Motschulsky)成虫粪便挥发性化合物中,筛选对该叶甲具有电生理及行为活性的化合物。【方法】通过GC-MS采集鉴定双斑长跗萤叶甲粪便挥发性化合物的组分和含量,利用触角电位仪和“Y”型嗅觉仪测定该叶甲雌、雄成虫对其粪便中4种挥发物的电生理和行为反应。【结果】该叶甲成虫粪便挥发物中含有包括烯类、醛类、酯类、酸类、醇类等共29种化合物;其雌、雄成虫均对α-石竹烯、α-蒎烯、α-红没药醇的EAG反应较明显,且雌雄虫均对10 μL/mL的α-红没药醇和α-蒎烯有较为明显的趋性。【结论】α-红没药醇和α-蒎烯可能为该叶甲成虫粪便中的有效活性成分。

关键词: 双斑长跗萤叶甲, 粪便挥发物, 电生理反应, 行为反应, 活性成分

CLC Number:

S435.62

LI Lun, YANG Chen, CHEN Jing. Identification of Active Components in Feces of Monolepta hieroglyphica (Motschulsky)[J]. Xinjiang Agricultural Sciences, 2020, 57(6): 1103-1112.

李伦, 杨陈, 陈静. 双斑长跗萤叶甲成虫粪便活性成分鉴定[J]. 新疆农业科学, 2020, 57(6): 1103-1112.

References

[1] 陈静, 张建萍, 张建华, 等. 双斑长跗萤叶甲的嗜食性[J]. 应用昆虫学报, 2007, 44(3): 357-360.
CHEN Jing, ZHANG Jianping, Zhang Jianhua, et al. Food preference of Monolepta hieroglyphica [J]. Chinese Bulletin of Entomology, 2007, 44(3): 357-360.
[2] 张聪, 袁志华, 王振营, 等. 双斑长跗萤叶甲在玉米田的种群消长规律[J]. 应用昆虫学报, 2014, 51(3): 668-675.
ZHANG Cong, YUAN Zhihua, WANG Zhenying, et al. Population dynamics of Monolepta hieroglyphica (Motschulsky) in cornfields [J]. Chinese Journal of Applied Entomology, 2014, 51(3): 668-675.
[3] 田永浩, 张建萍, 陈静, 等. 新疆棉花新害虫双斑长跗萤叶甲的发生特点及防治策略[J]. 安徽农学通报, 2007, 13(10): 120-121,230.
TIAN Yonghao, ZHANG Jianping, CHEN Jing, et al. Occurrence characteristics and preventions and control strategy of Monolepta hieroglyphica-a new pest of the cotton field in Xinjiang [J]. Anhui Agricultural Science Bulletin, 2007, 13(10): 120-121,230.
[4] 李广伟, 陈秀琳. 新疆棉区双斑长跗萤叶甲生活习性及消长动态调查研究[J]. 中国植保导刊, 2010, 30(6): 8-10.
LI Guangwei, CHEN Xiulin. Studies on biological characteristics and population dynamics of Monolepta hieroglyphica in cotton in Xinjiang [J]. China Plant Protection,2010, 30(6): 8-10.
[5] 陈光辉, 尹弯, 李勤, 等. 双斑长跗萤叶甲研究进展[J]. 中国植保导刊, 2016, 36(10): 19-26.
CHEN Guang-hui, YIN Wan, LI Qin, et al. Research progress on Monolepta hieroglyphica (Motschulsky) [J]. China Plant Protection, 2016, 36(10): 19-26.
[6] 姜勇, 雷朝亮, 张钟宁. 昆虫聚集信息素[J]. 昆虫学报, 2002, 45(6): 822-832.
JIANG Yong, LEI Chaoliang, ZHANG Zhongning. The aggregation pheromones of insects [J]. Acta Entomologica Sinica, 2002, 45(6): 822-832.
[7] McCaffery A R, Simpson S J, Islam M S, et al. A gregarizing factor present in the egg pod foam of the desert locust Schistocerca gregaria [J]. Journal of experimental Biology, 1998, 201(3): 347-363.
[8] Shi W P, Sun H L, Edward N, et al. Fecal volatile components elicit aggregation in the oriental migratory locust, Locusta migratoria manilensis (Orthoptera: Acrididae)[J]. Insect Science, 2011, 18(2): 166-174.
[9] Xu H, Turlings T C J. Plant volatiles as mate-finding cues for insects [J]. Trends in Plant Science, 2018, 23(2): 100-111.
[10] 王海建, 李彝利, 李庆, 等. 西藏飞蝗虫粪粗提物的成分分析及其活性测定[J]. 生态学报, 2013, 33(14): 4361-4369.
WANG Haijian, LI Yili, LI Qing, et al. Component analysis and bioactivity determination of fecal extract of Locusta migratoria tibetensis (Chen) [J]. Acta Ecologica Sinica, 2013, 33(14): 4361-4369.
[11] 王玉洁, 赵冬香, 曹海鹏. 小蠹虫信息素的研究与应用现状[J]. 生物安全学报, 2007, 16(4): 299-303.
WANG Yujie, ZHAO Dongxiang, CAO Haipeng. Research and application on bark beetles pheromone [J]. Journal of Biosafety, 2007, 16(4): 299-303.
[12] 雷高科, 符悦冠, 吴伟坚. 长小蠹聚集信息素研究概况[J]. 热带作物学报, 2019, 40(3): 616-622.
LEI Gaoke, FU Yueguan, WU Weijian. Aggregation Pheromones for Platypus (Coleoptera: Platypodidae) [J]. Chinese Journal of Tropical Crops, 2019, 40(3): 616-622.
[13] 刘晓梅, 凌冰, 张茂新. 跳甲聚集信息素的研究进展[J]. 环境昆虫学报, 2013, 35(5): 656-663.
LIU Xiaomei, LING Bing, ZHANG Maoxin. Research progress in aggregation pheromones of Alticinae[J]. Journal of Environmental Entomology, 2013, 35(5): 656-663.
[14] 程彬, 张健, 侯丽伟. 云杉八齿小蠹信息素的研究进展[J]. 环境昆虫学报, 2018, 40(2): 268-275.
CHENG Bin, ZHANG Jian, HOU Liwei. Advances in the studies on semiochemicals in Ips typographus [J]. Journal of Environmental Entomology, 2018, 40(2): 268-275.
[15] 陈青, 梁晓, 伍春玲, 等. 12种聚集信息素对橡胶小蠹虫的诱捕效果[J]. 热带作物学报, 2019, 40(12): 2432-2439.
CHEN Qing, LIANG Xiao, WU Chunling, et al. Trapping Effects of 12 Aggregation Pheromones on Rubber Bark Beetles[J]. Chinese Journal of Tropical Crops, 2019, 40(12): 2432-2439.
[16] 靳秀芳, 秦长生, 赵丹阳, 等. 象甲科昆虫性信息素与聚集信息素研究及应用进展[J]. 林业与环境科学, 2019, 35(3): 107-115.
JIN Xiufang, QIN Changsheng, ZHAO Danyang, et al. The Research and Application Progress of the Curculionidae Sex Pheromones and Aggregation Pheromone [J]. Forestry and Ecological Sciences, 2019, 35(3): 107-115.
[17] 泽桑梓, 闫争亮, 张真, 等. 华山松木蠹象聚集信息素分离鉴定和引诱效果[J]. 昆虫学报, 2010, 53(3): 293-297.
ZE Sangzi, YAN Zhengliang, ZHANG Zhen, et al. Identification and bioassay of aggregation pheromone components of Pissodes punctatus (Coleoptera: Curculionidae)[J]. Acta Entomologica Sinica, 2010, 53(3): 293-297.
[18] 范丽华, 牛辉林, 张金桐, 等. 脐腹小蠹聚集信息素的提取鉴定和引诱效果[J]. 生态学报, 2015, 35(3): 892-899.
FAN Lihua, NIU Huilin, ZHANG Jintong, et al. Extraction and identification of aggregation pheromone components of Scolytus schevyrewi Semenov(Coleoptera: Scolytidae) and trapping test[J]. Acta Ecologica Sinica, 2015, 35(3): 892-899.
[19] 张志虎, 郭丹丹, 陈静, 等. 双斑长跗萤叶甲对棉花和玉米10种挥发物的触角电生理反应[J]. 新疆农业科学, 2018, 55(2): 285-292.
ZHANG Zhihu, GUO Dandan, CHEN Jing, et al. Electrophysiological responses of the Monolepta hieroglyphica (Motschulsky) to 10 volatiles of cotton and maize [J]. Xinjiang Agricultural Sciences, 2018, 55(2): 285-292.
[20] 郭丹丹, 张志虎, 陈静, 等. 双斑长跗萤叶甲对棉花和玉米七种挥发物的EAG及行为反应[J]. 应用昆虫学报, 2018, 55(1): 79-86.
GUO Dandan, ZHANG Zhihu, CHEN Jing, et al. Electrophysiological and behavioral responses of Monolepta hieroglyphica (Motschulsky) to 7 cotton and corn volatiles [J]. Chinese Journal of Applied Entomology, 2018, 55(2): 285-292.
[21] Hassanali A, Njagi P G N, Bashir M O. Chemical ecology of locusts and related acridids[J]. Annu. Rev. Entomol., 2005, 50: 223-245.
[22] 郑卫青, 陈海婴, 柳小青, 等. 昆虫信息素研究与应用概况[J]. 中华卫生杀虫药械, 2014, 20(6): 591-594.
ZHENG Weiqing, CHEN Haiying, LIU Xiaoqing, et al. A brief review of the researching situation and application of insect pheromones [J]. Chinese Journal of Hygienic Insecticides & Equipments, 2014, 20(6): 591-594.
[23] 李会霞. 亚洲小车蝗体表和粪便挥发物及其电生理反应[D]. 呼和浩特:内蒙古农业大学, 2010.
LI Huixia. Physiological responses in the grasshopper Oedaleus asiaticus (Orthoptera:Acrididae) to volatiles from its live body and feces[D]. Hohhot: Inner Mongolia Agricultural University, 2010.
[24] 郭志永. 东亚飞蝗粪便挥发物中活性成分鉴定[D]. 北京:中国农业大学, 2004.
GUO Zhiyong. Identification of the Active Components from Fecal volatiles of Locusta migratoria manilensis[D]. Beijing: China Agricultural University, 2004.
[25] 石旺鹏, 闫跃英, 严毓骅. 东亚飞蝗不同来源聚集信息素的电生理活性[J]. 昆虫知识, 2003, 40(2): 145-149.
SHI Wangpeng, YAN Yueying, YAN Yuhua. EAG responses of Oriental migratory locust to semiochemicals from different sources of the insect [J]. Chinese Bulletin of Entomology, 2003, 40(2): 145-149.
[26] 陈静, 张建萍, 张建华, 等. 双斑长跗萤叶甲成虫在棉田的空间分布型及其抽样模型[J]. 石河子大学学报(自然科学版), 2007, 25(1): 39-42.
CHEN Jing, ZHANG Jianping, ZHANG Jianhua, et al. Spatial Distribution Pattern and Sampling Model for the Adults of Monolepta hieroglyphica in Cotton Fields[J]. Journal of Shihezi University (Natural Science Ed.), 2007, 25 (1): 39-42.
[27] 张增强. 八角尺蠖粪便及八角叶片提取物的生物活性研究[D]. 南宁:广西大学, 2011.
ZHANG Zengqiang. The Biological Activity of Extracts from Dilophodes elegans sinica Prout Frass and Illicium verum leaves [D]. Nanning: Guangxi University, 2011.
[28] 刘航, 迟德富, 陈海一, 等. 双斑长跗萤叶甲对几种植物挥发物的触角电位和行为反应[J]. 林业科学研究, 2013, 26(4): 488-493.
LIU Hang, CHI Defu, CHEN Haiyi, et al. EAG and behavioral responses of Monolepta hieroglyphica to several volatile compounds [J]. Forest Research, 2013, 26(4): 488-493.
[29] 张志虎, 陈静, 唐思琼, 等. 双斑长跗萤叶甲成虫对α-红没药醇等棉花和玉米挥发物的嗅觉行为反应[J]. 新疆农业科学, 2018, 55(6): 1117-1123.
ZHANG Zhihu, CHEN Jing, TANG Siqiong, et al. Olfactory Behavioral Response of the Monolepta hieroglyphica (Motschulsky) to volatiles of cotton and corn such as Dragosantol [J]. Xinjiang Agricultural Sciences, 2018, 55(6): 1117-1123.
[30] 樊慧, 金幼菊, 李继泉, 等. 引诱植食性昆虫的植物挥发性信息化合物的研究进展[J]. 北京林业大学学报, 2004, 26(3): 76-81.
FAN Hui, JIN Youju, LI Jiquan, et al. Advances on plant volatile semiochemicals attracting herbivorous insects [J]. Journal of Beijing Forestry University, 2004, 26(3): 76-81.
[31] 李倩. 桃蛀螟对玉米挥发性物质的电生理反应及触角超微结构观察[D]. 保定:河北农业大学, 2013.
LI Qian. Electrophysiological Responses to Corn Volatiles and Antennal Ultra-structure of Conogethes punctiferalis (Guenée)[D]. Baoding: Hebei Agricultural University, 2013.
[32] 任利利. 松墨天牛和天敌花绒寄甲对几种树木挥发物的电生理及行为反应[D]. 北京:北京林业大学, 2014.
REN Lili. Electrophysiological and behavioral responses of Monochamus alternatus and parasitoid Dastarcus helophorides to semiochemicals of several tree species [D]. Beijing: Beijing Forestry University, Beijing, 2014.
[33] 王佩星. 光肩星天牛对山核桃挥发物质的触角电生理反应[D]. 杭州:浙江农林大学, 2015.
WANG Peixing. EAG responses of Asian longhorn beetle Anoplophora glabripennis (Coleoptera:Cerambycidae) to Volatiles of Hickory [D]. Hangzhou: Zhejiang A & F University, 2015.
[34] 王茹琳, 杨伟, 杨佐忠, 等. 华山松大小蠹对几种寄主挥发物组分的EAG和行为反应[J]. 生态学杂志, 2011, 30(4): 724-729.
WANG Rulin, YANG Wei, YANG Zuozhong, et al. Electroantennographic and behavioral responses of Dendroctonus armandi (Coleoptera: Ipidae) to host plant volatiles [J]. Chinese Journal of Ecology, 2011, 30(4): 724-729.
[35] 杜家纬. 植物-昆虫间的化学通讯及其行为控制[J]. 植物生理学报, 2001, 27(3): 193-200.
DU Jiawei. Chemical communication between plants and insects and its behavior control [J]. Plant Physiology Journal, 2001, 27(3): 193-200.
[36] Pérez-Aparicio A, Torres-Vila L M, Gemeno C. EAG Responses of Adult Lobesia botrana Males and Females Collected from Vitis vinifera and Daphne Gnidium to Larval Host-Plant Volatiles and Sex Pheromone.[J]. Insects, 2019, 10(9): 281.
[37] 宁眺, 樊建庭, 方宇凌, 等. 不同危害状态下寄主萜烯挥发物含量的变化及松墨天牛对其组分的触角电位反应[J]. 昆虫学报, 2006, 49(2): 179-188.
NING Tiao, FAN Jianting, FANG Yuling, et al. Changes in contents of host volatile terpenes under different damaged states and electroantennogram response of Monochamus alternatus Hope to these volatiles [J]. Acta Entomologica Sinica, 2006, 49(2): 179-188.
[38] Yang G, Zhang Y N, Gurr G M, et al. Electroantennogram and behavioral responses of Cotesia plutellae to plant volatiles[J]. Insect Science, 2016, 23(2): 245-252.