Effects on Predation Response of Coccinella undecimpunctata Fed on Aphis gossypii Treated with Sublethal Doses of Flonicamid

doi:10.6048/j.issn.1001-4330.2021.10.012

Abstract

Abstract:

【Objective】 To provide a scientific basis for the rational use of pesticides and the cooperative biological control of Aphis gossypii by studying the effects on predation response of Coccinella undecimpunctata feeding on A. gossypii treated with sublethal doses of flonicamid. 【Methods】 According to the results of toxicity determination of LC₂₅ and LC₅₀ of flonicamid on A. gossypii, the influence of predation response of C. undecimpunctata feeding on A. gossypii treated with sublethal doses by the leaf soaking method were determined. 【Results】 The results showed that A. gossypii treated with flonicamid of different concentrations had certain influence on the predation function response and search effect of C. undecimpunctata, and with the increase of the concentration of the insecticides, the effect on the predation ability was greater. Especially after feeding A. gossypii treated with flonicamid LC₅₀, the daily maximum consumption and searching ability of C. undecimpunctata were significantly lower than that of CK, C. undecimpunctata‘s 1, 2, 3, 4 instars and adult instantaneous attack rate fell by 0.38%, 7.18%, 2.19%, 16.83% and 1.41%, the handling times required for a head of A. gossypii were extended the 0.002,4 d, 0.001,6 d, 0.000,6 d, 0.000,5 d and 0.000,1 d. The daily maximum consumption decreased by 0.72, 2.52, 7.76, 7.01 and 1.17 heads/dish, respectively. 【Conclusion】 The instantaneous attack rates of C. undecimpunctata of 1-4 instar larva and adult fed on A. gossypii treated with sublethal doses of flonicamid were reduced, the handling time was prolonged to certain degree, and the daily maximum predation amount decreased. Among them, A. gossypii treated with flonicamid LC₅₀ had the most obvious effect on the individual state of C. undecimpunctata.

Key words: Coccinella undecimpunctata; flonicamid; sublethal concentration; Aphis gossypii; predation response

摘要：

【目的】研究氟啶虫酰胺亚致死浓度处理棉蚜对十一星瓢虫捕食功能反应的影响,为合理使用杀虫剂和协同生物防治防控棉蚜提供科学依据。【方法】测定氟啶虫酰胺对棉蚜的毒力,以供试药剂的LC₂₅、LC₅₀为处理浓度,采用浸叶法处理棉蚜,选取活体棉蚜作为十一星瓢虫的食物,分析对其捕食功能反应的影响。【结果】饲喂氟啶虫酰胺不同亚致死浓度处理棉蚜后,十一星瓢虫捕食功能反应等参数及寻找效应均有一定影响,且随药剂浓度增加,对其捕食能力影响越大。饲喂氟啶虫酰胺LC₅₀处理的棉蚜后,十一星瓢虫日捕食量及搜寻能力显著降低,与对照相比,十一星瓢虫1、2、3、4龄幼虫和成虫的瞬时攻击率分别下降0.38%、7.18%、2.19%、16.83%、1.41%,处理一头棉蚜所用的时间分别延长0.002 4 d、0.001 6 d、0.000 6 d、0.000 5 d、0.000 1 d,日最大捕食量分别下降0.72、2.52、7.76、7.01、1.17头/皿。【结论】饲喂氟啶虫酰胺亚致死浓度处理棉蚜对十一星瓢虫1~4龄幼虫及成虫的瞬时攻击率降低,处理棉蚜所用时间延长,日最大捕食量下降。其中饲喂氟啶虫酰胺LC₅₀处理棉蚜对十一星瓢虫各虫态捕食功能有显著影响。

关键词: 十一星瓢虫, 氟啶虫酰胺, 亚致死浓度, 棉蚜, 捕食功能

CLC Number:

S476

Yindi WEI, Xue MA, Xu HAN, Zhenghui MA, Xiaoli ZHANG, Yongsheng YAO, Renci XIONG. Effects on Predation Response of Coccinella undecimpunctata Fed on Aphis gossypii Treated with Sublethal Doses of Flonicamid[J]. Xinjiang Agricultural Sciences, 2021, 58(10): 1860-1867.

魏引弟, 马雪, 韩旭, 麻正辉, 张笑丽, 姚永生, 熊仁次. 氟啶虫酰胺亚致死浓度处理棉蚜对十一星瓢虫捕食功能反应的影响[J]. 新疆农业科学, 2021, 58(10): 1860-1867.

Figures/Tables 4

References 26

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虫态 Stage	猎物密度 Prey density	处理 Pesticide
虫态 Stage	猎物密度 Prey density	CK	F LC₂₅	F LC₅₀
1龄幼虫 1st instar	5	2.80±0.20^a	2.80±0.20^a	2.80±0.20^a
	10	5.20±0.20^a	5.00±0.24^ab	4.80±0.20^ab
	15	6.80±0.37^a	6.60±0.40^a	6.80±0.24^a
	20	7.60±0.24^a	7.40±0.24^a	7.60±0.20^a
	25	7.80±0.20^a	7.60±0.24^a	7.80±0.24^a
2龄幼虫 2nd instar	10	6.20±0.20^a	5.80±0.20^ab	5.60±0.24^abc
	20	10.40±0.51^a	10.00±0.32^a	9.60±0.24^ab
	30	14.20±0.58^a	13.80±0.58^a	13.20±0.73^ab
	40	17.80±0.37^a	16.80±0.49^ab	15.80±0.37^bc
	50	19.00±0.32^a	17.80±0.20^ab	17.40±0.40^bc
3龄幼虫 3rd instar	30	15.60±0.24^a	15.20±0.20^a	15.00±0.20^a
	60	26.80±0.37^a	26.00±0.55^a	25.40±0.24^a
	90	35.00±0.32^a	34.20±0.58^ab	33.20±0.20^b
	120	46.00±0.32^a	45.00±0.32^a	44.60±0.24^a
	150	53.00±0.32^a	51.80±0.58^ab	50.40±0.51^b
4龄幼虫 4th instar	50	33.40±0.51^a	32.20±0.37^a	27.80±0.20^b
	90	43.00±0.45^a	41.00±0.32^b	37.80±0.20^c
	130	52.00±0.55^a	49.40±0.40^b	45.20±0.20^c
	170	69.00±0.32^a	66.20±0.37^b	59.20±0.37^c
	210	90.00±0.55^a	89.20±0.58^a	80.00±1.10^b
成虫 Adult	50	29.00±0.32^a	28.60±0.24^a	28.40±0.24^a
	90	40.60±0.24^a	40.20±0.20^a	40.20±0.20^a
	130	45.00±0.71^a	45.00±0.32^a	44.60±0.75^a
	170	62.80±0.37^a	61.00±0.84^ab	60.00±0.71^b
	210	75.00±0.32^a	74.80±0.37^a	74.40±0.24^a

虫态 Stage	猎物密度 Prey density	处理 Pesticide
虫态 Stage	猎物密度 Prey density	CK	F LC₂₅	F LC₅₀
1龄幼虫 1st instar	5	2.80±0.20^a	2.80±0.20^a	2.80±0.20^a
	10	5.20±0.20^a	5.00±0.24^ab	4.80±0.20^ab
	15	6.80±0.37^a	6.60±0.40^a	6.80±0.24^a
	20	7.60±0.24^a	7.40±0.24^a	7.60±0.20^a
	25	7.80±0.20^a	7.60±0.24^a	7.80±0.24^a
2龄幼虫 2nd instar	10	6.20±0.20^a	5.80±0.20^ab	5.60±0.24^abc
	20	10.40±0.51^a	10.00±0.32^a	9.60±0.24^ab
	30	14.20±0.58^a	13.80±0.58^a	13.20±0.73^ab
	40	17.80±0.37^a	16.80±0.49^ab	15.80±0.37^bc
	50	19.00±0.32^a	17.80±0.20^ab	17.40±0.40^bc
3龄幼虫 3rd instar	30	15.60±0.24^a	15.20±0.20^a	15.00±0.20^a
	60	26.80±0.37^a	26.00±0.55^a	25.40±0.24^a
	90	35.00±0.32^a	34.20±0.58^ab	33.20±0.20^b
	120	46.00±0.32^a	45.00±0.32^a	44.60±0.24^a
	150	53.00±0.32^a	51.80±0.58^ab	50.40±0.51^b
4龄幼虫 4th instar	50	33.40±0.51^a	32.20±0.37^a	27.80±0.20^b
	90	43.00±0.45^a	41.00±0.32^b	37.80±0.20^c
	130	52.00±0.55^a	49.40±0.40^b	45.20±0.20^c
	170	69.00±0.32^a	66.20±0.37^b	59.20±0.37^c
	210	90.00±0.55^a	89.20±0.58^a	80.00±1.10^b
成虫 Adult	50	29.00±0.32^a	28.60±0.24^a	28.40±0.24^a
	90	40.60±0.24^a	40.20±0.20^a	40.20±0.20^a
	130	45.00±0.71^a	45.00±0.32^a	44.60±0.75^a
	170	62.80±0.37^a	61.00±0.84^ab	60.00±0.71^b
	210	75.00±0.32^a	74.80±0.37^a	74.40±0.24^a

剂量 Dose	虫态 Stage	Holling-II圆盘方程 Holling-II equation	相关系数 Relate coefficient	瞬时攻击率 Instantaneous attack rate (α)	处理时间 (d) Handling time (T_h)	日最大捕食量 (head) Maximum consumption (Na_max)
CK	1龄幼虫	Na=0.678 1N/(1+0.038 4N)	0.989 4	0.678 1	0.056 6	17.67
LC₂₅		Na=0.688 1N/(1+0.042 9N)	0.992 8	0.688 1	0.062 3	16.05
LC₅₀		Na=0.674 3N/(1+0.039 8N)	0.994 1	0.674 3	0.059 0	16.95
CK	2龄幼虫	Na=0.725 7N/(1+0.017 7N)	0.997 9	0.725 7	0.024 4	40.98
LC₂₅		Na=0.673 3N/(1+0.016 3N)	0.998 7	0.024 5	0.675 0	40.82
LC₅₀		Na=0.653 9N/(1+0.017 0N)	0.999 4	0.653 9	0.026 0	38.46
CK	3龄幼虫	Na=0.594 8N/(1+0.005 1N)	0.996 9	0.594 8	0.008 5	117.65
LC₂₅		Na=0.578 3N/(1+0.005 0N)	0.996 7	0.578 3	0.008 7	114.94
LC₅₀		Na=0.572 9N/(1+0.005 2N)	0.995 3	0.572 9	0.009 1	109.89
CK	4龄幼虫	Na=0.868 1N/(1+0.007 1N)	0.916 1	0.868 1	0.008 2	121.95
LC₂₅		Na=0.832 3N/(1+0.007 1N)	0.900 2	0.832 3	0.008 5	117.65
LC₅₀		Na=0.699 8N/(1+0.006 1N)	0.938 4	0.699 8	0.008 7	114.94
CK	成虫	Na=0.763 2N/(1+0.007 0N)	0.943 0	0.763 2	0.009 2	108.70
LC₂₅		Na=0.751 3N/(1+0.006 9N)	0.949 0	0.751 3	0.009 2	108.70
LC₅₀		Na=0.749 1N/(1+0.007 0N)	0.949 0	0.749 1	0.009 3	107.53

剂量 Dose	虫态 Stage	Holling-II圆盘方程 Holling-II equation	相关系数 Relate coefficient	瞬时攻击率 Instantaneous attack rate (α)	处理时间 (d) Handling time (T_h)	日最大捕食量 (head) Maximum consumption (Na_max)
CK	1龄幼虫	Na=0.678 1N/(1+0.038 4N)	0.989 4	0.678 1	0.056 6	17.67
LC₂₅		Na=0.688 1N/(1+0.042 9N)	0.992 8	0.688 1	0.062 3	16.05
LC₅₀		Na=0.674 3N/(1+0.039 8N)	0.994 1	0.674 3	0.059 0	16.95
CK	2龄幼虫	Na=0.725 7N/(1+0.017 7N)	0.997 9	0.725 7	0.024 4	40.98
LC₂₅		Na=0.673 3N/(1+0.016 3N)	0.998 7	0.024 5	0.675 0	40.82
LC₅₀		Na=0.653 9N/(1+0.017 0N)	0.999 4	0.653 9	0.026 0	38.46
CK	3龄幼虫	Na=0.594 8N/(1+0.005 1N)	0.996 9	0.594 8	0.008 5	117.65
LC₂₅		Na=0.578 3N/(1+0.005 0N)	0.996 7	0.578 3	0.008 7	114.94
LC₅₀		Na=0.572 9N/(1+0.005 2N)	0.995 3	0.572 9	0.009 1	109.89
CK	4龄幼虫	Na=0.868 1N/(1+0.007 1N)	0.916 1	0.868 1	0.008 2	121.95
LC₂₅		Na=0.832 3N/(1+0.007 1N)	0.900 2	0.832 3	0.008 5	117.65
LC₅₀		Na=0.699 8N/(1+0.006 1N)	0.938 4	0.699 8	0.008 7	114.94
CK	成虫	Na=0.763 2N/(1+0.007 0N)	0.943 0	0.763 2	0.009 2	108.70
LC₂₅		Na=0.751 3N/(1+0.006 9N)	0.949 0	0.751 3	0.009 2	108.70
LC₅₀		Na=0.749 1N/(1+0.007 0N)	0.949 0	0.749 1	0.009 3	107.53

剂量 Dose	虫态 Stage	寻找效应方程 Search effect equation	相关系数(R) Relate coefficient
CK	1龄幼虫	S=0.678 1/(1+0.038 4N)	0.989 4
LC₂₅		S=0.688 1/(1+0.042 9N)	0.992 8
LC₅₀		S=0.674 3/(1+0.039 8N)	0.994 1
CK	2龄幼虫	S=0.725 7/(1+0.017 7N)	0.997 9
LC₂₅		S=0.673 3/(1+0.016 3N)	0.998 9
LC₅₀		S=0.653 9/(1+0.017 0N)	0.999 4
CK	3龄幼	S=0.594 8/(1+0.005 1N)	0.996 9
LC₂₅		S=0.578 3/(1+0.005 0N)	0.996 7
LC₅₀		S=0.572 9/(1+0.005 2N)	0.995 3
CK	4龄幼	S=0.868 1/(1+0.007 1N)	0.916 1
LC₂₅		S=0.832 3/(1+0.007 1N)	0.900 2
LC₅₀		S=0.699 8/(1+0.006 1N)	0.938 4
CK	成虫	S=0.763 2/(1+0.007 0N)	0.943 0
LC₂₅		S=0.751 3/(1+0.006 9N)	0.949 0
LC₅₀		S=0.749 1/(1+0.007 0N)	0.949 0