Physiological Responses of Seedlings of Different Apple Rootstocks to Salt-Alkali Complex Stress

doi:10.6048/j.issn.1001-4330.2021.09.016

Abstract

Abstract: 【Objective】 The salt-alkali tolerance of different apple rootstocks was comprehensively evaluated, and apple rootstocks suitable for cultivation in saline-alkali soil in southern Xinjiang were selected.【Method】 Using 7 four-year-old apple rootstocks of M. hupehensis (Pamp.) Rehder, M. xiaojinensis Cheng et Jiang, M. robusta, M. hupehensis, M. sieversii Roem, M. Chaenomeles, and M. baccata Borkh as the test materials. NaCl, Na₂SO₄, NaHCO₃, Na₂CO₃were mixed in the proportion of 1∶9∶9∶1, and 1/2 Hoagland nutrient solution was irrigated in a pot, simulating the mixed saline-alkaline stress of 0, 15, 30, 45 and 60 mmol/L. The chlorophyll content, relative conductivity, and proline content of the leaves of seven apple rootstocks under mixed salt-alkali stress were measured. The membership function method was used to comprehensively evaluate the salt tolerance of each rootstock.【Results】 With the increase of stress intensity, the chlorophyll content of each rootstock decreased, the relative conductivity and proline content showed an overall upward trend, the total soluble sugar content first increased and then decreased, the soluble protein content showed two trends of "increase decrease increase".【Conclusion】 The salt-tolerant ability of the seven tested apple rootstocks in order from strong to weak was M. robusta > M. sieversii Roem > M. hupehensis > M. xiaojinensis Cheng et Jiang > M. baccata Borkh > M. Chaenomeles > M. hupehensis (Pamp.) Rehder.

Key words: apple rootstock; salt-alkali complex stress; physiological index; membership function method

摘要： 【目的】综合评价不同苹果砧木的耐盐碱性,筛选适合新疆南疆盐碱土壤栽培的苹果砧木。【方法】以湖北海棠、小金海棠、八棱海棠、平邑甜茶、野苹果、白海棠、山定子7个4年生苹果砧木为材料,将NaCl、Na₂SO₄、NaHCO₃、Na₂CO₃按1∶9∶9∶1的比例混合,采用盆栽浇灌1/2 Hoagland营养液,模拟浓度为0、15、30、45、60 mmol/L的盐碱复合胁迫,测定7个苹果砧木在盐碱复合胁迫下叶片的叶绿素含量、相对电导率、脯氨酸含量等5项生理指标,利用隶属函数法综合评价各砧木的耐盐性。【结果】随着胁迫强度的增大,各砧木叶片叶绿素含量不断减小,相对电导率和脯氨酸含量总体呈上升趋势,可溶性总糖含量总体呈先上升后下降,可溶性蛋白含量呈先上升后下降和“升高—降低—升高”2种变化趋势。【结论】7种供试苹果砧木的耐盐碱能力由强到弱依次为八棱海棠>野苹果>平邑甜茶>小金海棠>山定子>白海棠>湖北海棠。

关键词: 苹果砧木, 盐碱复合胁迫, 生理指标, 隶属函数法

CLC Number:

S661.1

XU Panyun, LI Chunlan, SONG Jindi, WANG Xi, CHENG Jiabao, WU Yuxia, HE Tianming. Physiological Responses of Seedlings of Different Apple Rootstocks to Salt-Alkali Complex Stress[J]. Xinjiang Agricultural Sciences, 2021, 58(9): 1694-1703.

许盼云, 李春兰, 宋金迪, 王鑫, 程嘉宝, 吴玉霞, 何天明. 不同苹果砧木实生苗对盐碱复合胁迫的生理响应[J]. 新疆农业科学, 2021, 58(9): 1694-1703.

References

[1] Jia X M, Wang H, Svetla S, et al. Comparative physiological responses and adaptive strategies of apple Malus halliana to salt, alkali and saline-alkali stress[J]. Scientia Horticulturae, 2019, 245 : 154 - 162.
[2] 李超. 盐胁迫下不同抗盐苹果砧木响应的生理差异及褪黑素的缓解效应[D]. 杨凌 : 西北农林科技大学, 2012.
LI Chao. Physiological differences in response of different salt-tolerant apple rootstocks under salt stress and the mitigation effect of melatonin[D]. Yangling : Northwest A&F University, 2012.
[3] 云雪雪, 陈雨生. 国际盐碱地开发动态对我国的启示[J]. 国土与自然资源研究, 2020, (1) : 84 - 87.
YUN Xuexue, CHEN Yusheng. Enlightenment of the development of saline alkali land in the world to China[J]. Research on Land and Natural Resources, 2020, (1) : 84 - 87.
[4] 余洋, 谷海斌, 张丽, 等. 绿洲农田盐碱斑土壤盐分特征分析[J].中国农业大学学报, 2016, 21(3) : 89 - 95.
YU Yang, GU Haibin, ZHANG Li, et al. Analysis of soil salt characteristics in oasis farmland[J]. Journal of China Agricultural University, 2016, 21(3) : 89 - 95.
[5] 胡明芳, 田长彦, 赵振勇, 等. 新疆盐碱地成因及改良措施研究进展[J]. 西北农林科技大学学报(自然科学版), 2012, 40(10) : 111 - 117.
HU Mingfang, TIAN Changyan, ZHAO Zhenyong, et al. Salinization causes andresearch progress of technologies improving saline-alkali soil in Xinjiang[J]. Journal of Northwest A&F University (NaturalScience Edition), 2012, 40(10) : 111 - 117.
[6] 刘璐, 郭梁, 王景红, 等. 中国北方苹果主产地苹果物候期对气候变暖的响应[J]. 应用生态学报, 2020, 31(3) : 845 - 852.
LIU Lu, GUO Liang, WANG Jinghong, et al.Response of apple phenology to climate warming in the main apple producing areas of northern China[J]. Chinese Journal of Applied Ecology, 2020, 31(3) : 845 - 852.
[7] Alizadeh A, Alizade V, Xelilova X. Salinity effect on nutrient absorption in dwarf apple rootstocks (M. pumila)[J]. Scientific Report, 2013, 4 : 192 - 197.
[8] Jia X M, Zhu Y F, Zhang R, et al. Ionomic and metabolomic analyses reveal the resistance response mechanism to saline-alkali stress in Malus halliana seedlings[J]. Plant Physiology and Biochemistry, 2020, 147 : 77 - 90.
[9] 李艳华, 杨敏生, 王海英, 等. 树木耐盐生理研究进展[J]. 河北林果研究, 2000, 15(2) : 189 - 196.
LI Yanhua, YANG Minsheng, WANG Haiying, et al. Research progress on the physiology of tree salt tolerance[J]. Hebei Forest and Fruit Research, 2000, 15(2) : 189 - 196.
[10] 张瑞, 贾旭梅, 朱祖雷, 等. ‘烟富六号’苹果在不同砧木上响应盐碱胁迫的光合及生理特性[J]. 果树学报, 2019, 36(6) : 718 - 728.
ZHANG Rui, JIA Xumei, ZHU Zulei, et al. Photosynthesis and physiological characteristics of yanfu 6 under saline-alkali stress on different rootstocks[J]. Journal of Fruit Science, 2019, 36(6) : 718 - 728.
[11] Sadia R, Ghulam A, Muhammad S, et al. Effect of salinity on cadmium tolerance, ionic homeostasis and oxidative stress responses in conocarpus exposed to cadmium stress: Implications for phytoremediation[J]. Ecotoxicology and Environmental Safety, 2019, 171 : 146 - 153.
[12] Shi D C, Sheng Y M. Effect of various salt-alkaline mixed stress conditions on sunflower seedlings and analysis of their stress factors[J]. Environmental and Experimental Botany, 2005, 54(1) : 8 - 21.
[13] 朱燕芳. 混合盐碱胁迫下苹果砧木的抗性生理和蛋白组学分析[D]. 甘肃 : 甘肃农业大学, 2018.
ZHU Yanfang. Analysis of resistance physiology and proteomics of apple rootstocks under mixed salt-alkali stress[D]. Gansu : Gansu Agricultural University, 2018.
[14] Yang J Y, Zhang W, Tian y, et al. Effects of various mixed salt-alkaline stresses on growth, photosynthesis, and photosynthetic pigment concentrations of Medicago ruthenica seedlings[J]. Photosynthetica, 2011, 49(2) : 275 - 284.
[15] 林冰冰, 韩振海, 王忆, 等. 苹果实生砧木种质资源耐缺铁和耐盐碱性评价[J]. 中国农业大学学报, 2016, 21(1) : 48 - 58.
LIN Bingbing, HAN Zhenhai, WANG Yi, et al. Evaluation of the germplasm resources of apple seedling rootstocks for tolerance to iron deficiency and salt and alkaline[J]. Journal of China Agricultural University, 2016, 21(1) : 48 - 58.
[16] 邹琦. 植物生理学实验指导[M]. 北京 : 中国农业出版社, 2000.
ZOU Qi. Guide of plant physiology experiment[M]. Beijing : China Agriculture Press, 2000.
[17] 高俊凤, 孙群, 曹翠玲, 等. 植物生理学试验指导[M]. 北京: 高等教育出版社, 2006.
GUO Junfeng, SUN Qun, CAO Cuiling, et al. Guidance of plant physiological experiment[M]. Beijing : Higher Education Press, 2000.
[18] 刘竞择, 曹柠, 张艳霞, 等. 葡萄砧木冬季抗抽干能力及抗旱性综合评价[J]. 果树学报, 2020, 37(3) : 339 - 349.
LIU Jingze, CAO Ning, ZHANG Yanxia, et al. Comprehensive evaluation of anti-drying ability and drought resistance of grape rootstocks in winter[J]. Journal of Fruit Science, 2020, 37(3) : 339 - 349.
[19] Zhang J H, Guo S J, Guo P Y, et al. The interacting effect of urea and fenoxaprop-P-ethyl on photosynthesis and chlorophyll fluorescence in Perilla frutescens[J]. Photosynthetica, 2014, 52(3) : 456 - 463.
[20] Chutipaijit S, Cha-um S, Sompornpailin K. High contents of proline and anthocyanin increase protective response to salinity in Oryza sativa L. spp. indica[J]. Australian Journal of Crop Science, 2011, 5(10) : 1191 - 1198.
[21] Gao Z W, Han J Y, Mu C S, et al. Effects of saline and alkaline stresses on growth and physiological changes in oat (Avena sativa L.) seedlings[J]. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2014, 42(2) : 357 - 362.
[22] 鞠淼. 盐及盐碱混合条件对燕麦胁迫作用的比较[D]. 长春 : 东北师范大学, 2008.
JU Miao. Comparison of the effects of salt and salt-alkali mixed conditions on oats stress[D]. Changchun : Northeast Normal University, 2008.
[23] Lu Y, Duan B L, Li C. Physiological responses to drought and enhanced UV-B radiation in two contrasting picea asperata populations[J]. Canadian Journal of Forest Research, 2007, 37(7) : 1253 - 1262.
[24] Khan M A, Ungar I A, Showalter A M The effect of salinity on the growth, water status, and ion content of a leaf succulent perennial halophyte, Suadea fruticosa (L.) Forssk[J]. Journal of Arid Environments, 2000, 45(1) : 73 - 84.
[25] Stepien P, Kobus G. Water relations and photosynthesis in Cucumis sativus L. leaves under salt stress[J]. Biologia Plantarum, 2006, 50(4) : 610 - 616.
[26] 刘晓涵, 张晓帆, 李雪利, 等. 烟草幼苗对复合盐碱的生理响应[J]. 中国烟草学报, 2020, 26(1) : 66 - 74.
LIU Xiaohan, ZHANG Xiaofan, LI Xueli, et al. Physiological response of tobacco seedlings to complex salt-alkali[J]. Acta Tobacco Sinica, 2020, 26(1) : 66 - 74.
[27] 谢阳娇. 几种引进园林树木对干旱和盐碱胁迫的生理响应及抗性评价研宄[D]. 北京:北京林业大学, 2018.
XIE Yangjiao.Research on physiological response and resistance evaluation of several introduced garden trees to drought and salt-alkali stress[D].Beijing:Beijing Forestry University, 2018.
[28] Ashraf M, Akram N A, Al-Qurainy F, et al. Drought tolerance: roles of organic osmolytes, growth regulators, and mineral nutrients[M]. United States : Academic Press, 2011.
[29] Vyrides I, Stuckey D C. Compatible solute addition to biological systems treating waste/wastewater to counteract osmotic and other environmental stresses: a review[J]. Critical Reviews in Biotechnology, 2017, 37(7) : 1 - 15.
[30] Turkan I. Plant responses to drought and salinity stress: developments in a post-genomic era[M]. United States : Academic Priess, 2011.
[31] Liang W J, Ma X L, Wan P, et al. Plant salt-tolerance mechanism: A review[J]. Biochemical and Biophysical Research Communications, 2018, 495(1) : 286 - 291.
[32] Kishor P B K, Sangam S, Amrutha R N, et al. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance[J]. Current Science, 2005, 88 : 424 - 438.
[33] Verbruggen N, Hermans C. Proline accumulation in plants: a review[J]. Amino Acids, 2008, 35 : 753 - 759.
[34] Guo R, Yang Z Z, Li F, et al, Comparative metabolic responses and adaptive strategies of wheat (Triticum aestivum) to salt and alkali stress[J]. BMC Plant Biology, 2015, 15 : 170.
[35] Blackman S A, Obendorf R L, Leopold A C, et al. Maturation proteins and sugars in desiccation tolerance of developing soybean seeds[J]. Plant Physiology. 1992, 100 : 225 - 230.
[36] Szabados L L, Savourcb A. Proline: a mulifunctional amino acid[J]. Trends in Plants Science, 2010, 15(2) : 89 - 97.
[37] Natarajan S K, Zhu W D, Liang X W, et al. Proline dehydrogenase is essential for proline protection against hydrogen peroxide induced cell death[J]. Free Radical Biology and Medicine, 2012, 53(5) : 1181 - 1191.
[38] 张正仁, 宋长铣. 脯氨酸的代谢[J]. 南京大学学报, 1987, 23(3) : 430 - 441.
ZHANG Zhengren, SONG Changxian. Metabolism of proline[J]. Journal of Nanjing University, 1987, 23(3) : 430 - 441.