Xinjiang Agricultural Sciences ›› 2019, Vol. 56 ›› Issue (10): 1828-1838.DOI: 10.6048/j.issn.1001-4330.2019.10.008

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Construction of High Density Genetic Map and QTL Mapping for Downy Mildew Traits in Cucumis melo ssp.melo

ZHANG Xue-jun1,2,YANG Wen-li1,Zhang Yong-bing1,Zhang Jian2,Guo Li-xia1,YANG Yong1,LI Mei-hua1,YI Hong-ping1,2   

  1. 1.The Research Center of Hami-melon ,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;
    2.Quzhou xinnongdu academician workstation, Quzhou Zhejiang 324022, China
  • Received:2019-07-25 Published:2019-11-22
  • Correspondence author: YI Hong-ping(1962-), male, researcher, The research direction is on the genetic and breeding of western melon and melon, (E-mail)xjyhp2010@sina.com.

采用GBS-seq技术构建甜瓜高密度遗传图谱

张学军1,2, 杨文莉1, 张永兵1, 张健2, 郭丽霞1, 杨永1, 李寐华1, 伊鸿平1,2   

  1. 1.新疆农业科学院哈密瓜研究中心,乌鲁木齐 830091;
    2.衢州新农都院士工作站,浙江衢州 324022
  • 通讯作者: 伊鸿平(1962-),男,福建武夷山人,研究员,硕士,研究方向为西甜瓜遗传育种,(E-mail)xjyhp2010@sina.com
  • 作者简介:张学军(1980-),男,吉林四平人,副研究员,硕士,研究方向为西甜瓜抗病育种,(E-mail)zxj333@126.com
  • 基金资助:
    新疆维吾尔自治区自然科学基金(2015211A027)

Abstract: 【Objective】 To construct high-density genetic linkage map of muskmelon and improve the positio- ing accuracy for the study of genetic mapping of muskmelon.【Method】 In this study, were constructed F2 isolated populations crossed by the melon materials K7-4 (highly resistant to downy mildew) and K7-2 (highly susceptible to downy mildew). The library building and high-throughput sequencing of F2 population were conducted by genotype by sequencing method (GBS).We used SAM TOOLS software, and obtained the high throughput SNP markers of the populations.Using sliding Windows method , the genotyping of the F2 p opulation was carried out,Joinmap 4.0 maximum likelihood (ML) method was used for the mapping, R/ QTL genetic distance was calculated (kosambi mapping function), linkage map was drawn with perl SVG module, and R/ QTL software package and composite interval mapping model were used for QTL mapping.【Result】 The total length of the high-density genetic map was4,823.55 cM, and the average genetic distance between bin markers was 1.43 cM. Totally, 26 QTLS were identified for downy mildew traits in seedling, middle and late stages.【Conclusion】 The high-density genetic map of melon was obtained based on GBS-seq technology, and the resistance downy mildew gene was finally linked to the CM3.5.1_scaffold00005 of 6,831,227 -7,830,935 bp, within the range of about 1Mb.

Key words: Cucumis melo ssp.melo; GBS; High density genetic map; downy mildew; QTL

摘要: 【目的】构建甜瓜高密度遗传图谱,为研究甜瓜重要性状基因定位及功能分析奠定基础。【方法】以甜瓜材料K7-4(高抗霜霉病)和K7-2(高感霜霉病)为亲本,杂交再自交得到 F2分离群体。构建GBS文库并进行双末端测序,使用滑动窗口的方法进行基因型分型,SAM TOOLS软件检测SNP位点,采用Joinmap 4.0软件进行排图,用perl SVG模块绘制连锁图,用win QTL cart 2.5进行QTL检验,根据复合区间作图法,使用R/qtl软件包进行QTL定位。【结果】构建了总长为4 823.55 cM的遗传图谱,标记间平均遗传距离为1.43 cM。在苗期、生长中期和生长后期共检测到26个与甜瓜霜霉病性状相关的QTL。【结论】基于GBS-seq技术获得了甜瓜高密度遗传图谱,最终将抗霜霉病基因关联到CM3.5.1_scaffold00005上的6,831,227~7,830,935 bp,约1Mb的区间范围内。

关键词: 甜瓜, GBS, 遗传图谱, 霜霉病, QTL