Identification of Pathogenic Fungi Causing the Root Rot Disease of Dabancheng Faba Bean and Analysis of Antagonistic Effect by the Biocontrol Bacteria

doi:10.6048/j.issn.1001-4330.2019.010.016

Abstract

Abstract: 【Objective】 To identify pathogens causing root rot disease in Dabancheng faba bean and analyze antagonistic effects by the biocontrol bacteria.【Method】 Diseased plants of Dabancheng faba bean were collected, and pathogenic fungi were isolated and identified by the molecular method. And also, the inhibitory effects of biocontrol bacteria on pathogenic fungi were tested.【Result】 The results showed the root rot disease of Dabancheng faba bean was caused by Fusarium chlamydosporum var. fuscum. Pathogenicity analysis displayed that it had strong infectivity to Dabancheng faba bean and pathogenicity could reach 100%. Both of biocontrol bacteria B6 and B1 had good inhibitory effect on the pathogen fungus, and the rate of inhibition zone to colony could reach 1.90.【Conclusion】 Fusarium chlamydosporum var. fuscum causing the root rot disease of Dabanchen faba bean was reported firstly, which, meanwhile, provided the scientific basis and theory for efficient biological control of faba bean root rot.

Key words: faba bean; root rot disease; pathogen; biocontrol bacteria

摘要： 【目的】研究鉴定达坂城蚕豆根腐病病原菌,分析生防菌对病原菌的拮抗效果,为高效生物防治提供了科学依据和理论基础。【方法】采集达坂城蚕豆根腐病病株,进行病原菌分离及微生物分子鉴定,分析生防菌拮抗效果。【结果】达坂城蚕豆根腐病病原菌为厚孢镰孢菌变种(Fusarium chlamydosporum var. fuscum),其对达坂城蚕豆具有很强的侵染性,可达到100%致病。所采用的生防菌B6和B1均对病原菌株具有较好的抑制效果,其最大抑菌圈菌落比可达到1.90。【结论】达坂城蚕豆根腐病病原菌为厚孢镰孢菌变种(Fusarium chlamydosporum var. fuscum)。

关键词: 蚕豆, 根腐病, 病原菌, 生防菌

CLC Number:

:S435.23

CHU Min, GU Mei-Ying, TANG Qi-yong, ZHU Jing, HAO Xiu-ying, ZHANG Zhi-dong. Identification of Pathogenic Fungi Causing the Root Rot Disease of Dabancheng Faba Bean and Analysis of Antagonistic Effect by the Biocontrol Bacteria[J]. Xinjiang Agricultural Sciences, 2019, 56(10): 1904-1911.

楚敏, 顾美英, 唐琦勇, 朱静, 郝秀英, 张志东. 达坂城蚕豆根腐病病原菌鉴定及生防菌拮抗效果分析[J]. 新疆农业科学, 2019, 56(10): 1904-1911.

References

[1]段晓东.达坂城蚕豆根腐病的流行因子及综合防控研究[J]. 基层农业推广,2017,5(7):42-45.;DUAN Xiao-dong.Study on the epidemic factors and integrated control of root rot of Vicia faba in Dabancheng [J]. Primary Agricultural Technology Extension, 2017,5(7):42-45.
[2] 俞大绂. 蚕豆病害[M].北京:科学出版社,1979.;Yu Da-fuFaba bean diseases [M]. Beijing:Science Press,1979.
[3] 张丽荣.浅谈达坂城区地理标志蚕豆的发展现状[J]. 农民致富之友,2017,(8):62.;ZHANG Li-rong.Development status of geographical indication broad bean in Dabancheng District [J]. Nongmin Zhifuzhiyou Yuekan, 2017,(8):62.
[4] 任泉,蔡新婷,马文惠,等.新疆达坂城地区52a来气温和降水变化特征分析[J]. 干旱区资源与环境,2011,25(10):116-121.;REN Quan,CAI Xin-ting,MA Wen-hui,et al. Changing features of temperature and precipitation in recent 52 years in Dabancheng region [J]. Journal of Arid Land Resources and Environment,2011,25(10):116-121.
[5] 李彦忠, 聂红霞. 甘肃省红豆草病原真菌鉴定及病害发生动态调查[J]. 植物保护学报, 2016, 43(2):222-232.;LI Yan-zhong,NIE Hong-xia. The causal agents of sainfo in fungal diseases and disease dynamics in Gansu Province [J]. Journal of Plant Protection, 2016,43(2):222-232.
[6] 董艳, 董坤, 汤利,等. 蚕豆根系分泌物中氨基酸含量与枯萎病的关系[J]. 土壤学报,2015,52(4):919-925.;DONG Yan, DONG Kun, TANG Li, et al. Relationship of free amino acids in root exudates with wilt disease (fusarium oxysporum)of faba bean[J]. Acta Pedologica Sinica,2015,52(4):919-925.
[7] Kim, O. S., Cho, Y. J. , Lee, K. , Yoon, S. H. , Kim, M. , & Na, H. , et al. Introducing eztaxon-e: a prokaryotic 16s rrna gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol, 2012,62: 716-721.
[8] 李阳, 李健强, 宋素琴,等. 高放射土壤中拮抗放线菌菌株的筛选及鉴定[J]. 新疆农业科学, 2017, 54(1):132-139.;LI Yang, LI Jian-qiang , SONG Su-qin, et al. The Screening and Identification of Antagonistic Actinomycetes Strains from the High R adiation Soil [J]. Xinjiang Agricultural Sciences, 2017, 54(1):132-139.
[9] Ghule, M. R. , Sawant, I. S. , Sawant, S. D. , Sharma, R. , & Shouche, Y. S. Identification of fusarium species as putative mycoparasites of plasmopara viticola causing downy mildew in grapevines. Australasian Plant Disease Notes, 2018,13(1):16.
[10] 任海英,陈志谊,许志刚,等.常熟地区蚕豆枯萎病病原菌鉴定及其致病力初探[J].植物保护,2003,(4):30-32.;REN Hai-yin, CHEN Zhi-yi, XU Zhi-gang, et al. Study on main species and virulence of faba bean Fusarium wilt in Changshu aera[J].Plant Protection, 2003,(4):30-32.
[11] 李春杰,南志标.临夏地区春蚕豆根腐病发生与为害调查[J].植物保护,1996,(6):25-26.;LI Chun-jie, NAN Zhi-biao. Investigation on occurrence and damage of spring silkworm root rot in Linxia area [J].Plant Protection,1996, (6):25-26.
[12] 鲍建荣,王拱辰,叶琪明.浙江省蚕豆镰刀菌病害的病原种类及其分析[J].浙江农业大学学报,1992,18(3):61-64.;BAO Jian-rong, WANG Gong-zheng, YE Qi-ming. Analysis on Fusarium pathogens on faba bean in Zhejiang Province [J]. Journal of Zhejiang Agricultural University,1992,18(3):61-64.
[13] 常敬华, 何志明, 李高峰. 河北、内蒙古地区小麦镰刀菌的分离与鉴定[C]//.中国菌物学会2015年学术年会论文摘要集. 2015:200.;CHANG Jin-hua, HE Zhi-ming, LI Gao-feng. Isolation and identification of Fusarium graminearum from wheat in Hebei and Inner Mongolia[C]//.Abstracts of academic papers of Chinese mycological society in 2015:200 .
[14] 刘怀宇.玉米镰刀菌型茎腐、穗腐、苗期根腐病的相互关系及防治[J].农民致富之友,2018,(7):150.;LIU Huai-yu. Relationship and control of Fusarium root rot, ear rot and seedling root rot in Maize [J]. Nongmin Zhifuzhiyou Yuekan, 2018,(7):150.
[15] 张亚朵, 刘佳, 黄文坤,等. 河北廊坊大豆枯萎病病原镰刀菌的分子鉴定[J]. 植物病理学报,2018.;ZHANG Ya-duo, LIU Jia, HUANG Wen-kun, et al. Molecular identification of Fusarium species from the wilt soybean lines in Langfang, Hebei Province [J]. Acta Phytopathologica Sinica, 2018.
[16] Gupta, V. K. , & Misra, A. K. Fusarium chlamydosporum, causing wilt disease of guava (psidium guajava l.) in india. Archives of Phytopathology and Plant Protection, 2012,45(20):2,425-2,428.
[17] Jian, L. I., Yan, L. I. , Xing-Xiang, G., Feng, F. , & Mei, L. I. The biological characteristics of digitaria sanguinalis pathogen fusarium chlamydosporum strain ZC201301. Acta Prataculturae Sinica, 2016,3(25):234-239.
[18] Trabelsi, R., Gdoura, R., & Triki, M. A. Fusarium Brachygibbosum and Fusarium Chlamydosporum Causing Wilt and Die-back of Olive in Tunisia. Euro-Mediterranean Conference for Environmental Integration. Springer, Cham, 2017.
[19] F. R., Shi, N. N., & Ruan, H. C. First report of fusarium chlamydosporum causing banana crown rot in Fujian Province, china. Plant Disease, 2017,101(6):1,048.