Analysis on Process Optimization about Accelerated Solvent Extraction of Main Components in Cordyceps militaris

doi:10.6048/j.issn.1001-4330.2018.05.017

Xinjiang Agricultural Sciences ›› 2018, Vol. 55 ›› Issue (5): 928-935.DOI: 10.6048/j.issn.1001-4330.2018.05.017

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Analysis on Process Optimization about Accelerated Solvent Extraction of Main Components in Cordyceps militaris

CHEN Fang-yuan^1,2, MA Mai-yan^1,2, ZHANG Xiang-feng¹, MA Zheng-hai², JIAO Zi-wei¹

1.College of Biological and Geographic Sciences, Yili Normal University, Yining Xinjiang 835000, China;
2. College of life Science and Technology, Xinjiang University, Urumqi 830046, China

Online:2018-05-20 Published:2018-07-25
Correspondence author: JIAO Zi-wei(1973-),male, Ph.D., professor, research for selection and artificial cultivation of edible fungi, and machenism of microorganism promotion,(E-mail)741285332@qq.com; ZHANG Xiang-feng(1976-), male, master, lecturer, research for selection and artificial cultivation of edible fungi, (E-mail) 759737497@qq.com
Supported by:
Autonomous Region Technology Program of Supporting Xinjiang " Key technology integration and product development of agricultural standardization production of green facility agriculture in Yili, Xinjiang "(2017E0239); Microbiological Key Platform Program of Yili Normal University: " Analysis on different exogenous selenium treatments to main effective components and fruiting body of Cordyceps militars" (YLUKLM201606)

加速溶剂萃取法提取蛹虫草主要成分工艺优化分析

陈方圆^1,2,马麦燕^1,2,张相锋¹,马正海²,焦子伟¹

1.伊犁师范学院生物与地理科学学院,新疆伊宁 835000;
2. 新疆大学生命科学学院,乌鲁木齐 830046

通讯作者: 焦子伟(1973-),男,博士,教授,研究方向为药食用真菌选育及人工栽培、微生物促生机理,(E-mail)741285332@qq.com
张相锋(1976-),男,硕士,讲师,研究方向为药食用真菌选育及人工栽培,(E-mail)759737497@qq.com
作者简介:陈方圆(1990-),女,河南人,硕士,研究方向为药食用真菌活性成分提取分析,(E-mail)
基金资助:
自治区科技支疆项目“新疆伊犁绿色设施农业标准化生产关键技术集成及产品开发”(2017E0239);微生物重点平台项目“不同外源外源硒对蛹虫草子实体影响及主要有效成分分析”(YLUKLM201606)

Abstract

Abstract: 【Objective】 Cordyceps militaris contains many kinds of active substances such as nucleosides, polysaccharides, cordycepin, sterols, sugar alcohols, enzymes, pigments and other active substances, among which cordycepin, adenosine, polysaccharide and so on are widely studied.【Method】 The extraction of cordycepin and adenosine was commonly used by ultrasonic method, reflux method, percolation extraction method and so on. In this paper, accelerated solvent extraction was used to obtain the active substances of cordycepin and adenosine from the fruiting bodies of Cordyceps militaris. The optimum conditions were obtained by the method of orthogonal experiment and the rapid extraction method. The method of accelerated solvent extraction was optimized by orthogonal experiment. In addition, four factors (temperature, static extraction time, ethanol concentration and cycle number) were designed to determine the optimal combination conditions.【Result】 The optimum combination conditions for the extraction of cordycepin and adenosine by accelerated extraction were obtained by experiments.【Conclusion】 The optimum conditions for the extraction of adenosine by accelerated extraction were as follows: temperature 70℃, time 5 min, ethanol concentration 20% and cycle times 2, and the optimum conditions for extracting adenosine were as follows: temperature 100℃, time 10 min, ethanol content 0 and cycle times 2 times.

Key words: Cordyceps militaris; cordycepin; adenosine; extraction; accelerated solvent extraction

摘要： 【目的】蛹虫草(Cordyceps militaris)成分内含有大量的核苷类、多糖、虫草酸、甾醇、糖醇、酶和色素等多种活性物质,其中对虫草素(cordycepin)、腺苷(adenosine)、多糖等研究广泛。【方法】目前提取虫草素、腺苷常用超声法、回流法、渗漉提取法等。采取加速溶剂萃取法,从蛹虫草子实体中获取虫草素、腺苷活性物质,并进行四因素(温度、静态萃取时间、乙醇浓度和循环次数)三水平正交试验设计,明确其最优组合条件。【结果】通过试验得到了加速萃取法提取虫草素、腺苷的最佳组合条件。【结论】加速萃取法提取虫草素的最适条件是:温度70℃,时间5 min,乙醇浓度20%,循环次数2次;提取腺苷的最适条件是:温度100℃,时间10 min,乙醇含量0,循环次数2次。

关键词: 蛹虫草, 虫草素, 腺苷, 提取, 加速溶剂萃取法

CLC Number:

CHEN Fang-yuan, MA Mai-yan, ZHANG Xiang-feng, MA Zheng-hai, JIAO Zi-wei. Analysis on Process Optimization about Accelerated Solvent Extraction of Main Components in Cordyceps militaris[J]. Xinjiang Agricultural Sciences, 2018, 55(5): 928-935.

陈方圆, 马麦燕, 张相锋, 马正海, 焦子伟. 加速溶剂萃取法提取蛹虫草主要成分工艺优化分析[J]. 新疆农业科学, 2018, 55(5): 928-935.

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Analysis on Process Optimization about Accelerated Solvent Extraction of Main Components in Cordyceps militaris

加速溶剂萃取法提取蛹虫草主要成分工艺优化分析

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