基于非靶向代谢组学分析气调包装对精河枸杞鲜果采后代谢物的影响

doi:10.6048/j.issn.1001-4330.2025.08.018

新疆农业科学 ›› 2025, Vol. 62 ›› Issue (8): 1996-2011.DOI: 10.6048/j.issn.1001-4330.2025.08.018

• 园艺特产·微生物·贮藏保鲜加工 • 上一篇下一篇

基于非靶向代谢组学分析气调包装对精河枸杞鲜果采后代谢物的影响

李圣杰¹, 郑素慧^2,3,4, 韩蓉¹, 孙天罡⁵, 潘俨^2,3,4

1. 新疆农业大学食品科学与药学学院, 乌鲁木齐 830091;
2. 新疆维吾尔自治区农业科学院农产品加工研究所, 乌鲁木齐 830091;
3. 新疆主要农副产品精深加工工程技术研究中心, 乌鲁木齐 830091;
4. 新疆农产品加工与保鲜重点实验室, 乌鲁木齐 830091;
5. 精河县枸杞产业发展中心, 新疆精河 833300

收稿日期:2025-01-20 发布日期:2025-12-06
作者简介:李圣杰（1997—），男，新疆石河子人，硕士研究生，研究方向农产品加工及贮藏工程，（E-mail）1048213107@qq.com
基金资助:
新疆维吾尔自治区重点研发计划项目“新疆枸杞优质干燥与保鲜贮运关键技术研发与示范”（2023B02009-2）

Effects of air-conditioned packaging on postharvest metabolites of Jinghe Lycium barbarum L. fresh fruits analyses based on untargeted metabolomics

LI Shengjie¹, ZHENG Suhui^2,3,4, HAN Rong¹, SUN Tiangang⁵, PAN Yan^2,3,4

1. College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China;
2. Institute of Agricultural Products Processing, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
3. Research Center of Main Agricultural Products Processing Engineering in Xinjiang, Urumqi 830091, China;
4. Key Laboratory of Agricultural Products Processing and Preservation in Xinjiang, Urumqi 830091, China;
5. Jinghe County Wolfberry Industry Development Center, Jinghe Xinjiang 833300, China

Received:2025-01-20 Online:2025-12-06
Supported by:
The Major Scientific R & D Program Special Project of Xinjiang Uygur Autonomous Region "R & D of Key Technologies for HighQuality Drying and Fresh-Keeping Storage and Transportation of Xinjiang Wolfberry"（2023B02009-2）

摘要/Abstract

摘要： 【目的】采用非靶向代谢组学技术研究低温条件下气调包装（MAP）处理精河枸杞鲜果的代谢物差异，分析比较对照（CK）与MAP枸杞鲜果在贮藏期间代谢物的组成。【方法】以新疆精河枸杞鲜果为试材，利用高效液相色谱串联质谱进行非靶向代谢组学分析，鉴定和比较不同包装枸杞鲜果的代谢物差异。【结果】2种包装枸杞鲜果中共检测出1 550种代谢物，包括脂类和类脂类、有机酸及其衍生物、有机杂环类和苯类等15类。通过多元分析，共筛选出387种差异代谢物，包含142种上调代谢物、245种下调代谢物，以有机酸类、脂类、苯类和杂环类为主。KEGG富集分析发现，2种包装枸杞鲜果的差异代谢物主要以氨基酸代谢和碳水化合物代谢通路为主。【结论】不同包装对精河枸杞鲜果采后代谢物的含量影响较大，可为枸杞鲜果的营养品质、生物多样性及包装方式的多样化发展提供理论参考。

关键词: 精河枸杞; 气调包装; 鲜果; 采后代谢物; 非靶向代谢组学

Abstract: 【Objective】To study the differences in metabolites of Jinghe Lycium barbarum L. fresh fruits treated with modified atmosphere packaging（MAP）under low temperature conditions by using nontargeted metabolomics technology, and analyze and compare the composition of metabolites of CK and MAP Lycium barbarum L. fresh fruits during storage.【Methods】The fresh fruits of Lycium barbarum L. fresh in Jinghe, Xinjiang were used as test materials, and high performance liquid chromatography tandem mass spectrometry was used for non-targeted metabolomics analysis to identify and compare the differences in metabolites of fresh fruits of Lycium barbarum L. fresh in different packaging.【Results】A total of 1550 metabolites were detected in the two packaged Lycium barbarum L. fruits, including 15 categories of lipids and lipids, organic acids and their derivatives, organic heterocyclics and benzenes. Through multivariate analysis, a total of 387 differential metabolites were screened, including 142 up-regulated metabolites and 245 downregulated metabolites, mainly organic acids, lipids, benzenes and heterocycles. KEGG enrichment analysis found that the differential metabolites of the two packaged Lycium barbarum L. fruits were mainly amino acid metabolism and carbohydrate metabolism pathways.【Conclusion】Different packaging has a great influence on the content of metabolites in fresh fruits of Jinghe Lycium barbarum L. fresh fruits, which can provide theoretical reference for the nutritional quality, biodiversity and diversified development of packaging methods of Lycium barbarum fruits.

Key words: Jinghe Lycium barbarum L.; modified atmosphere packaging; fresh fruits; postharvest metabolites; metabolomics

中图分类号:

S609

李圣杰, 郑素慧, 韩蓉, 孙天罡, 潘俨. 基于非靶向代谢组学分析气调包装对精河枸杞鲜果采后代谢物的影响[J]. 新疆农业科学, 2025, 62(8): 1996-2011.

LI Shengjie, ZHENG Suhui, HAN Rong, SUN Tiangang, PAN Yan. Effects of air-conditioned packaging on postharvest metabolites of Jinghe Lycium barbarum L. fresh fruits analyses based on untargeted metabolomics[J]. Xinjiang Agricultural Sciences, 2025, 62(8): 1996-2011.

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基于非靶向代谢组学分析气调包装对精河枸杞鲜果采后代谢物的影响

Effects of air-conditioned packaging on postharvest metabolites of Jinghe Lycium barbarum L. fresh fruits analyses based on untargeted metabolomics

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