加工番茄系统抗性诱导促生菌的筛选鉴定及其促生防病效果

doi:10.6048/j.issn.1001-4330.2022.06.018

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (6): 1466-1474.DOI: 10.6048/j.issn.1001-4330.2022.06.018

加工番茄系统抗性诱导促生菌的筛选鉴定及其促生防病效果

林青¹(), 史应武¹, 王娜², 华兰兰³, 杨红梅¹, 楚敏¹, 曾军¹, 高雁¹, 霍向东¹()

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091
2.新疆大学生命科学与技术学院,乌鲁木齐 830046
3.新疆师范大学生命科学学院,乌鲁木齐 830054

收稿日期:2021-10-15 出版日期:2022-06-20 发布日期:2022-07-07
通信作者: 霍向东(1974-),男,甘肃人,副研究员,研究方向为微生物资源,(E-mail) xiangdonghuo@163.com
作者简介:林青(1985-),女,山东人,硕士,助理研究员,研究方向为微生物生态,(E-mail) qinglinxj@163.com
基金资助:
新疆维吾尔自治区自然科学基金“介导加工番茄诱导系统抗性促生菌的筛选鉴定及其抗病效能的研究”(2018D01B43)

Screening and Identification of Systemic Resistant Inducing Growth-Promoting Bacteria and Their Effects on Growth and Disease Prevention of Processing Tomatoes

LIN Qing¹(), SHI Yingwu¹, WANG Na², HUA Lanlan³, YANG Hongmei¹, CHU Min¹, ZENG Jun¹, GAO Yan¹, HUO Xiangdong¹()

1. Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences/ Xinjiang Laboratory of Special Environmental Microbiology, Urumqi, Xinjiang 830091, China
2. College of Life Science and Technology,Xingjiang University,Urumqi, Xinjiang 830046, China
3. School of Life Sciences,Xinjiang Normal University, Urumqi, Xinjiang 830054, China

Received:2021-10-15 Published:2022-06-20 Online:2022-07-07
Supported by:
Natural Science Foundation Project of Xinjiang Uygur Autonomous Region "Screening and Identification of Microbia with Growth-Promoting, System Resistance Induction of Processing Tomato, and Their Efficiencies Research"(2018D01B43)

摘要/Abstract

摘要：

【目的】 筛选具有诱导番茄系统抗性的促生菌并验证其对加工番茄早疫病、灰霉病的防效,为新疆加工番茄病害的生物防治提供理论依据和菌种资源。【方法】 采用稀释涂布法,分离番茄等作物的根部土壤细菌,以无菌生理盐水和稀释至10⁴倍数未接菌的NB培养基为空白对照,利用发芽实验初筛加工番茄促生菌;用菌株发酵液灌根处理加工番茄幼苗,以清水处理为空白对照,测量叶片中茉莉酸含量,复筛系统抗性诱导菌株,分别挑战接种番茄早疫病、灰霉病病原菌,统计疫情指数和诱抗效果。测定菌株16S rDNA序列,初步进行菌种鉴定。【结果】 共分离到147株细菌,筛选得到19株显著(P<0.05)促进加工番茄种子萌发、增加根长的促生菌,有4株促生菌显著(P<0.05)提高加工番茄叶片中茉莉酸含量,防病验证最终得到3株能同时有效防治加工番茄早疫病、灰霉病的菌株,诱抗防效在27.59%~39.44%。菌株FY10、FY12、FY93鉴定为Bacillus atrophaeus,Pseudomonas wadenswilerensis和Bacillus pumilus。【结论】 获得3株具有系统抗性诱导功能且有效防治加工番茄早疫病、灰霉病的促生菌。

关键词: 系统抗性诱导; 促生菌; 加工番茄; 早疫病; 灰霉病

Abstract:

【Objective】 The purpose of the research is to screen the growth-promoting bacteria that can induce tomato systemic resistance and verify its control effect against early blight and gray mold of processing tomato, so as to provide theoretical basis and bacterial resources for the biological control of processing tomato diseases in Xinjiang.【Method】 The soil bacteria were isolated by the dilution coating terile normal saline and NB medium diluted to 10⁴times without inoculation as blank control, the germination experiment was used to initially screen processing tomato growth-promoting bacteria.After irrigating root of tomato seedlings with strain fermentation liquid,clean water treatment as a blank jasmonic acid content in the leaves was measured to re-screen the systemic resistance inducing disease index and induced effect counted after inoculation with the pathogen of tomato early blight and gray mold 16S rDNA sequence was determined to identify the strain initially.【Results】 A total of 147 strains of bacteria were isolated, and 19 strains were screened to significantly (P<0.05) promote the germination and root growth of processed tomato seeds, and 4 strains significantly (P<0.05) increased the content of jasmonic acid in processing tomato,3 strains were obtained, which effectively control early blight and gray mold of processed tomato at the same time with the induced effect between 27.59% and 39.44%.Strain FY10, FY12, and FY93 were preliminarily identified as Bacillus atrophaeus, Pseudomonas wadenswilerensis and Bacillus pumilus.【Conclusion】 3 growth-promoting bacteria obtained with the ability to induce systemic resistance and effectively control early blight and gray mold of processed tomato.

Key words: resistance induction; growth-promoting bacteria; processed tomato; early blight; gray mold

中图分类号:

S188

林青, 史应武, 王娜, 华兰兰, 杨红梅, 楚敏, 曾军, 高雁, 霍向东. 加工番茄系统抗性诱导促生菌的筛选鉴定及其促生防病效果[J]. 新疆农业科学, 2022, 59(6): 1466-1474.

LIN Qing, SHI Yingwu, WANG Na, HUA Lanlan, YANG Hongmei, CHU Min, ZENG Jun, GAO Yan, HUO Xiangdong. Screening and Identification of Systemic Resistant Inducing Growth-Promoting Bacteria and Their Effects on Growth and Disease Prevention of Processing Tomatoes[J]. Xinjiang Agricultural Sciences, 2022, 59(6): 1466-1474.

图/表 6

表1 不同作物根际和根区土壤分离细菌数量

Table 1 The number of bacteria isolated from rhizosphere and root zone soil of different crops

	样本数量 Number of samples	根际土壤细菌数量 The number of bacteria in rhizosp here soil	根区土壤细菌数量 The number of bacteria in root zone soil
番茄 Tomato	15	48	32
辣椒 Chilli Pepper	5	24	12
豆角 Beans	4	19	12

图1 加工番茄促生菌的促生效果注:A:加工番茄种子根长;B:加工番茄种子发芽率;C:加工番茄种子发芽势

Fig.1 Growth-promoting effect of processing tomato growth-promoting bacteria Note:A: Root length of processing tomato seeds; B: Germination rate of processing tomato seeds; C: Germination potential of processing tomato seeds

图2 菌株诱导接种后加工番茄幼苗茉莉酸含量

Fig.2 Jasmonic acid content of processing tomato seedlings after induction of strains

图3 基于16SrDNA序列构建的系统发育树

Fig.3 Phylogenetic tree based on the 16S rDNA sequence

图4 诱导菌株与茄链格孢菌Alternaria solani(A)灰葡萄孢菌Botrytis cinerea(B)的拮抗作用

Fig.4 Antagonism of induced strains with Alternaria solani (A) and Botrytis cinerea(B)

表2 诱导菌株对加工番茄早疫病、灰霉病的诱抗效果

Table 2 Inducing effect of induced strains on early blight and gray mold of processing tomato

菌株 Strain	早疫病 Early blight				灰霉病 Gray mold
	病情指数 Disease index (%)		诱抗效果 Induced effect(%)		病情指数 Disease index(%)		诱抗效果 Induced effect (%)
	5 d	14 d	5 d	14 d	5 d	14 d	5 d	14 d
CK	16.00^d	50.67^c	-	-	16.67^b	56.00^c	-	-
FY10	4.67^b	34.67^b	70.57^b	31.54^b	2.00^a	35.33^a	88.33^a	36.67^a
FY12	1.33^a	30.67^a	92.13^c	39.44^c	4.67^a	39.33^b	88.33^a	29.63^a
FY93	3.33^ab	36.67^b	78.90^bc	27.59^b	4.00^a	38.00^ab	75.48^a	31.85^a
FY136	8.00^c	51.33^c	50.07^a	-1.33^a	3.33^a	36.67^ab	78.81^a	34.32^a

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加工番茄系统抗性诱导促生菌的筛选鉴定及其促生防病效果

Screening and Identification of Systemic Resistant Inducing Growth-Promoting Bacteria and Their Effects on Growth and Disease Prevention of Processing Tomatoes

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