不同地理种群棉蚜三种关键性酶活差异及其与抗性相关性

doi:10.6048/j.issn.1001-4330.2022.08.022

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (8): 2007-2013.DOI: 10.6048/j.issn.1001-4330.2022.08.022

• 植物保护·农产品分析检测·农业装备工程与机械化 • 上一篇下一篇

不同地理种群棉蚜三种关键性酶活差异及其与抗性相关性

王小丽¹(), 韩睿¹, 张全成¹, 赵鹏程², 王俊刚¹()

1.石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用重点实验室,新疆石河子 832003
2.新疆天禾嘉信农业科技有限公司,乌鲁木齐 830000

收稿日期:2021-10-15 出版日期:2022-08-20 发布日期:2022-10-01
通信作者: 王俊刚（1970-）,男,甘肃秦安人,教授,博士,研究生导师,研究方向为植物保护,（E-mail） wangjungang98@163.com
作者简介:王小丽（1997-）,女,新疆昌吉人,硕士研究生,研究方向为植物保护,（E-mail） 1436647660@qq.com
基金资助:
国家自然科学基金“棉长管蚜诱导棉花防御反应对棉长管蚜和棉蚜种间竞争影响”(31660519);国家自然科学基金“缩节胺调控棉花防御棉蚜的生化机制研究”(31260435)

Study on the Differences of Three key Enzyme Activities of Different Geographical Populations of Aphis gossypii in Xinjiang and Their Correlation with Resistance

WANG Xiaoli¹(), HAN Rui¹, ZHANG Quancheng¹, ZHAO Pengcheng², WANG Jungang¹()

1. College of Agriculture/Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi Xinjiang 832003, China
2. Xinjiang Tianhe Jiaxin Agricultural Technology Co., Ltd., Urumqi 830000,China

Received:2021-10-15 Published:2022-08-20 Online:2022-10-01
Correspondence author: WANG Jungang（1970-）, male, native place: Taian Gansu, Professor and master tutor of Shihezi University, research field： plant protection,（E-mail） wangjungang98@163.com
Supported by:
Regional Science Foundation projects “The Cotton Defense Response Induced by Acyrthosiphom gossypii on Interspecific Competition between Acyrthosiphom gossypii and Aphis gossypii ”(31660519);Regional Science Foundation projects “Biochemical Mechanism of Cotton Resistant to Aphis gossypii by mepiquat chloride ”(31260435)

摘要/Abstract

摘要：

【目的】研究新疆不同地理种群棉蚜3种酶的活性及抗药性的差异。【方法】测定新疆库尔勒、五家渠、石河子、奎屯、哈密地区棉蚜和实验室敏感种群羧酸酯酶、谷胱甘肽-S-转移酶、乙酰胆碱酯酶活性,并分析3种酶活性与吡虫啉抗性之间的关系。【结果】库尔勒、五家渠、石河子、奎屯和哈密地区乙酰胆碱酯酶和谷胱甘肽-S-转移酶的比活力比值分别为4.2、2.7、2.3、2.5、2.1倍和2.3、1.5、1.3、2.5、2.1倍,差异显著（P<0.05）;羧酸酯酶比活力比值为6.5、4.2、3.4、2.7、1.2倍,除哈密地区相对接近外,其余地区均明显高于1.0,差异显著（P<0.05）,2种棉蚜关键解毒酶和1种神经传导关键性酶与抗药性之间存在一定相关性。【结论】各地理种群棉蚜3种酶活性均显著高于敏感种群（P<0.05）,且彼此之间存在差异性,羧酸酯酶在棉蚜感受到吡虫啉杀虫剂胁迫时极有可能起到重要作用。

关键词: 棉蚜; 羧酸酯酶; 乙酰胆碱酯酶; 谷胱甘肽-S-转移酶; 抗药性

Abstract:

【Objective】Objective to explore the difference of three enzyme activities and drug resistance of different geographical populations of Aphis gossypii in Xinjiang. 【Methods】 The activities of carboxylesterase, glutathione-S-transferase and acetylcholinesterase of Aphis gossypii and laboratory sensitive populations in Korla, Wujiaqu, Shihezi, Kuitun and Hami of Xinjiang were determined, and the relationship between the activities of the three enzymes and imidacloprid resistance was analyzed. 【Results】 The specific activity ratios of acetylcholinesterase and glutathione-S-transferase in Korla, Wujiaqu, Shihezi, Kuitun and Hami were 4.2, 2.7, 2.3, 2.5, 2.1 times and 2.3, 1.5, 1.3, 2.5, 2.1 times, respectively（P < 0.05）; the specific activity ratios of carboxylesterase were 6.5, 4.2, 3.4, 2.7, 1.2 times, except Hami, the other areas were significantly higher than 1.2 times There was a certain correlation between two key detoxification enzymes and one key neurotransmitter enzyme of Aphis gossypii and drug resistance.【Conclusion】 The results showed that the activities of three kinds of enzymes in different populations were significantly higher than those in sensitive populations（P <0.05）, and there were differences among them. Carboxylesterase may play an important role in the process of Aphis gossypii feeling imidacloprid stress.

Key words: Aphis gossypii; carboxylesterase; acetylcholinesterase; glutathione-S-transferase; drug resistance

中图分类号:

王小丽, 韩睿, 张全成, 赵鹏程, 王俊刚. 不同地理种群棉蚜三种关键性酶活差异及其与抗性相关性[J]. 新疆农业科学, 2022, 59(8): 2007-2013.

WANG Xiaoli, HAN Rui, ZHANG Quancheng, ZHAO Pengcheng, WANG Jungang. Study on the Differences of Three key Enzyme Activities of Different Geographical Populations of Aphis gossypii in Xinjiang and Their Correlation with Resistance[J]. Xinjiang Agricultural Sciences, 2022, 59(8): 2007-2013.

图/表 4

表 1 不同地理种群棉蚜的羧酸酯酶比活力

Table 1 CarE activities in wingless adults of different geographic populations of Aphis gossypii

棉蚜种群 Population	CarE比活力 Specific activity of CarE （U /mgpro-min）	CarE比活力比值 Dynamic ratio of CarE
SS	0.236±0.006 ^d	1.0
奎屯 Kurtun	0.647±0.116 ^c	2.7
石河子 SHi hezi	0.808±0.002 ^bc	3.4
哈密 Ha mi	0.284±0.002 ^d	1.2
五家渠 Wujaqu	0.992±0.153 ^b	4.2
库尔勒 Kuerle	1.359±0.119 ^a	6.5

表 2 不同地理种群棉蚜的乙酰胆碱酯酶比活力

Table 2 AchE activities in wingless adults of different geographic populations of Aphis gossypii

棉蚜种群 Population	CarE比活力 Specific activity of CarE （U /mgpro-min）	AchE比活力比值 Dynamic ratio of AchE
SS	2.997±0.034 ^f	1.0
奎屯 Kurtun	7.346±0.030 ^c	2.5
石河子 SHi hezi	6.950±0.028 ^d	2.3
哈密 Ha mi	6.146±0.016 ^e	2.1
五家渠 Wujaqu	8.196±0.044 ^b	2.7
库尔勒 Kuerle	12.570±0.051 ^a	4.2

表 3 不同地理种群棉蚜的谷胱甘肽-S-转移酶比活力

Table 3 GST activities in wingless adults of different geographic populations of Aphis gossypii

棉蚜种群 Population	CarE比活力 Specific activity of CarE （U /mgpro-min）	GST比活力比值 Dynamic ratio of GST
SS	3.986±0.035 ^f	1.0
奎屯 Kurtun	10.080±0.343 ^a	2.5
石河子 SHi hezi	5.242±0.035 ^e	1.3
哈密 Ha mi	8.445±0.323 ^c	2.1
五家渠 Wujaqu	6.143±0.097 ^d	1.5
库尔勒 Kuerle	9.150±0.106 ^b	2.3

图 1 不同地理种群棉蚜对吡虫啉抗性与3种酶活性的相关性注：图中数据为平均数±标准差。柱上不同小写字母表示同地区不同棉蚜体内酶活差异显著（P<0.05）。

Fig. 1 Relationship between imidaclopridresistance to Aphis gossypii and three enzyme activities in different geographical populations Note： The data in the figure are mean ± standard deviation. Different lowercase letters on the column indicated that there were significant differences in enzyme activities among different Aphis gossypii in the same area（P<0.05）.

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不同地理种群棉蚜三种关键性酶活差异及其与抗性相关性

Study on the Differences of Three key Enzyme Activities of Different Geographical Populations of Aphis gossypii in Xinjiang and Their Correlation with Resistance

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