新疆部分地区牛源空肠弯曲菌分离鉴定及耐药性分析

doi:10.6048/j.issn.1001-4330.2017.09.020

摘要/Abstract

摘要： 【目的】研究新疆部分地区牛空肠弯曲菌污染及耐药性。【方法】从乌鲁木齐、昌吉、石河子3个地区共采集牛肛拭子171份。用布氏肉汤初步增菌后,用改良CCD琼脂基础和哥伦比亚血平板2种选择培养基培养,用聚合酶链式反应(PCR)鉴定,最后参照2010年美国临床实验室标准化研究所(CLSI)推荐使用的琼脂扩散法进行药敏试验。【结果】从乌鲁木齐牛场肛拭子分离鉴定到1株空肠弯曲菌(编号为N22),检出率为1.11%(1/90);昌吉牛场肛拭子分离鉴定到1株空肠弯曲菌(编号为C25),检出率为3.33%(1/30)。石河子牛场肛拭子没有分离到空肠弯曲菌。2株空肠弯曲菌对单环β内酰胺类、氨基糖苷类抗生素高度敏感;对喹诺酮类抗菌药物、大环内酯类和大部分头孢菌素类抗生素产生了不同程度的耐药。【结论】新疆牛群存在空肠弯曲菌的感染,感染菌株可用单环β-内酰胺类、氨基糖苷类抗生素进行防治。研究结果可为评价新疆牛群空肠弯曲菌的流行状况和制定防控措施提供科学依据。

关键词: 空肠弯曲菌; PCR; 分离鉴定; 耐药性检测

Abstract: 【Objective】 To study the prevalence of campylobacter jejuni from cattle in partial areas of Xinjiang and its drug resistance.【Method】In this experiment, 171 fecal samples were collected from cattle in Urumqi, Changji and Shihezi. After the initial enrichment of Brucella broth, the culture medium was cultured with two improved media, namely, the improved CCD agar base and the Columbia blood plate, and finally identified by polymerase chain reaction (PCR). Finally, the agar diffusion method recommended by the United States Clinical Laboratory Standardization Institute (CLSI) in 2010 was used for drug susceptibility test.【Result】A strain of Campylobacter jejuni (No. N22) was isolated from the Urumqi cattle farm and the detection rate was 1.11%(1/90). A strain of Campylobacter jejuni (No.C25) was identified from Changji cattle farmand the detection rate was 3.33%(1/30).There were no strains of Campylobacter jejuni isolated from Shihezi cattle farm. 2 strains of Campylobacter jejuni were highly sensitive to monocyclic beta lactam and aminoglycoside antibiotics,and showing different degrees ofresistenceto quinolones, macrolides and most of the cephalosporins antibiotics.【Conclusion】The infection of campylobacter jejuni was found from cattle in Xinjiang, and the infection strains could be used to prevent and cure with monobactam and aminoglycoside antibiotics. The results can provide a scientific basis for the evaluation of the prevalence of campylobacter jejuni and the prevention and control measures in Xinjiang cattle herds.

Key words: campylobacter jejuni; PCR; isolation and identification; drug resistance test

中图分类号:

代婧, 彭斌, 雷程红, 阿热阿依·海依拉提. 新疆部分地区牛源空肠弯曲菌分离鉴定及耐药性分析[J]. 新疆农业科学, 2017, 54(9): 1730-1736.

DAI Jing, PENG Bin, LEI Chen-hong, Aray Hayrat. Isolation,Identification and Drug Resistance Detection of Campylobacter Jejuni from Cattle in Partial Areas of Xinjiang[J]. Xinjiang Agricultural Sciences, 2017, 54(9): 1730-1736.

参考文献

[1] 翟海华. 青岛地区鸡源空肠弯曲菌流行病学、ERIC-PCR分型及毒力基因研究[D].泰安:山东农业大学硕士学位论文,2014.
   ZHAI Hai-hua. (2014). Epidemiology, ERIC-PCR typing and virulence genes of Campylobacter jejuni in chicken farms in Qingdao [D]. Master Thesis. Shandong Agricultural University, Taian. (in Chinese)
[2] 翟海华,王娟,王君伟,等. 空肠弯曲菌的致病性及致病机制研究进展[J]. 动物医学进展,2013,(12):164-169.
   ZHAI Hai-hua, WANG Juan, WANG Jun-wei, et al. (2013). Pathogenicity and Pathogenesis of Campylobacter jejuni [J]. Advances in Animal Medicine, (12): 164-169. (in Chinese)
[3] Altekruse, S. F., Stern, N. J., Fields, P. I., & Swerdlow, D. L. (1999). Campylobacter jejuni-an emerging foodborne pathogen. Emerging Infectious Diseases, 5(1): 28-35.
[4] Allos, B. M. (2001). Campylobacter jejuni infections: update on emerging issues and trends. Clinical Infectious Diseases, 32(8): 1,201.
[5] 许紫建. 猪、鸡弯曲菌的分离鉴定和耐药性分析及空肠弯曲菌LAMP检测方法的研究[D].保定:河北农业大学硕士学位论文,2013.
   XU Zi-jian. (2013). Analysis of Isolation and Identification of Candida spp. And Analysis of Drug Resistance and LAMP Detection Method of Campylobacter jejuni[D]. Master Thesis. Hebei Agricultural University,Baoding. (in Chinese)
[6] 朱冬梅.肉鸡屠宰过程中弯曲菌的分离鉴定、耐药性及PFGE分析[D].成都:四川农业大学硕士学位论文,2014.
   ZHU Dong-mei. (2014). Isolation, identification and drug resistance of PFM in broiler slaughtering process [D]. Master Thesis. Sichuan Agricultural University,Chengdu. (in Chinese)
[7] 郑扬云.食品中空肠弯曲菌的污染分布规律及遗传多样性研究[D].广州:广东工业大学硕士学位论文,2013.
   ZHENG Yang-yun.(2013). Study on the Distribution Law and Genetic Diversity of Campylobacter jejuni in Food [D]. Master Thesis. Guangdong University of Technology, Guangzhou. (in Chinese)
[8] 刘夏阳.弯曲菌的分离培养及分型分析[D].北京:中国疾病预防控制中心硕士学位论文,2014.
   LIU Xia-yang. (2014). Isolation and culture of Campylobacter and its typing analysis [D]. Master Thesis.China Center for Disease Control and Prevention, Beijing. (in Chinese)
[9] Vanniasinkam, T., Lanser, J. A., & Barton, M. D. (1999). Pcr for the detection of campylobacter spp. in clinical specimens. Letters in Applied Microbiology, 28(1): 52-56.
[10] 韩新锋,刘书亮,张晓利,等.鸡肉空肠弯曲杆菌的分离鉴定及耐药性分析[J]. 中国人兽共患病学报,2012,(1):31-36.
   HAN Xin-feng, LIU Shu-liang, ZHANG Xiao-li, et al. (2012). Isolation and identification of Campylobacter jejuni in chicken and analysis of drug resistance [J]. Chinese Journal of Zoology, (1):31-36. (in Chinese)
[11] Cockerill, F. R. (2010). Performance standards for antimicrobial susceptibility testing : twentieth informational supplement. Performance standards for antimicrobial susceptibility testing: twenty-third informational supplement. Clinical and Laboratory Standards Institute.
[12] Headrick ML, Korancy S, Bean NH, et al. (1998). Profile of raw milk-associated food borne disease outbreaks reported in the United States, 1973 through 1992. American journal of public health, 88(8):1,219-1,221.
[13] 吴蜀豫,张立实,冉陆. 弯曲菌及弯曲菌病的流行现状[J]. 中国食品卫生杂志,2004,(1):58-61.
   WU Shu-yu, ZHANG Li-shi, RAN Lu. (2004). The prevalence of Campylobacter and Campylobacter disease [J]. Chinese Journal of Food Hygiene, (1):58-61. (in Chinese)
[14] 陈志新,吕德生,万绍平, 等. 儿童空肠弯曲菌感染的流行病学研究[J]. 中华预防医学杂志,1995,(3):144-146.
   CHEN Zhi-xin, LÜ De-sheng,WAN Shao-ping, et al.(1995). Epidemiological study of Campylobacter jejuni infection in children [J]. Chinese Journal of Preventive Medicine, (3):144-146. (in Chinese)
[15] 黄金林,许海燕,郑国军,等. 部分省市鸡群空肠弯曲菌和结肠弯曲菌的流行状况分析[J]. 中国预防兽医学报,2008,(1):72-75.
   HUANG Jin-lin, XU Hai-yan, ZHENG Guo-jun, et al.(2008). Analysis of epidemic situation of Campylobacter jejuni and Campylobacter jejuni in some provinces and cities [J]. Journal of Preventive Veterinary Medicine, (1):72-75. (in Chinese)
[16] 吴光先. 不同季节鸡、猪肠道中嗜热弯曲菌群携带状况的调查[J]. 中国人兽共患病杂志,1990,(3):44.
   WU Guang-xian.(1990) Investigation on the carrying status of thermophilic benthic strains in chickens and pigs in different seasons [J] . Chinese Journal of Zoonoses, (3):44. (in Chinese)
[17] 张弓. 水禽空肠弯曲菌流行病学及分离株的多位点序列分型分析[D]. 扬州: 扬州大学硕士学位论文,2010.
   ZHANG Gong. (2010). Epidemiology of Campylobacter jejuni in waterfowl and analysis of multi - locus sequence of isolates [D]. Master Thesis. Yangzhou University,Yangzhou. (in Chinese)
[18] 吴忠亮. 空肠弯曲杆菌感染分布研究[D]. 上海:上海交通大学硕士学位论文,2007.
   WU Zhong-liang. (2007). Distribution of Campylobacter jejuni infection [D]. Master Thesis. Shanghai Jiaotong University, Shjanghai. (in Chinese)
[19] 谢永强,钟华敏,虢艳,等.广州地区儿童空肠弯曲菌感染的检测与分析[J]. 国际检验医学杂志,2016,(17):2 448-2 450.
   XIE Yong-qiang, ZHONG Hua-min, GUO Yan, et al. (2016). Detection and analysis of Campylobacter jejuni infection in children in Guangzhou [J]. Journal of International Journal of Laboratory Medicine, (17):2,448-2,450. (in Chinese)
[20] 侯凤琴,沈宝铨,孙新婷. 200株空肠弯曲菌对30种抗生素敏感性研究[J]. 医师进修杂志,2001,(3):39-40.
   HOU Feng-qin, SHEN Bao-quan, SUN Xin-ting. (2001). Study on the susceptibility of 200 strains of Campylobacter jejuni to 30 antibiotics [J]. Journal of Physician Training, (3):39-40.(in Chinese)

编辑推荐 0

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	0	0	0	75

来源	本网站	其他网站

次数	12	63
比例	16%	84%

摘要

292

最新录用	在线预览	正式出版

0	0	292

来源	本网站	其他网站

次数	176	116
比例	60%	40%