海棠感染苹果茎痘病毒发生与检测

doi:10.6048/j.issn.1001-4330.2024.12.021

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (12): 3061-3066.DOI: 10.6048/j.issn.1001-4330.2024.12.021

• 草业·植物保护·畜牧兽医 • 上一篇下一篇

海棠感染苹果茎痘病毒发生与检测

张李娅¹(), 刘保军¹, 单佳祁¹, 王澍¹, 顾爱星¹(), 白剑宇²()

1.新疆农业大学农学院,乌鲁木齐 830052
2.新疆林业科学院经济林研究所,乌鲁木齐 830063

收稿日期:2024-04-10 出版日期:2024-12-20 发布日期:2025-01-16
通信作者: 顾爱星( 1973- ),女,新疆人,教授,博士,硕士生/博士生导师,研究方向为棉花抗病育种,(E-mail)gax@xjau.edu.cn；
白剑宇( 1977- ),男,河北人,研究员,研究方向为林业有害生物监测预警与防控,(E-mail)bjyhao2010@sina.com
作者简介:张李娅( 1999- ),女,云南人,硕士研究生,研究方向为植物病理学,(E-mail)2768296845@qq.com
基金资助:
国家林业和草原局林业科技专项“新疆森林、草原、湿地生态系统外来入侵植物病原微生物普查”项目(65000022P00789110010X)

Occurrence and detection of begonia infected by apple stem pitting virus

ZHANG Liya¹(), LIU Baojun¹, SHAN Jiaqi¹, WANG Shu¹, GU Aixing¹(), BAI Jianyu²()

1. College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China
2. Research Institute of Economic Forestry, Xinjiang Academy of Forestry Sciences, Urumqi 830063, China

Received:2024-04-10 Published:2024-12-20 Online:2025-01-16
Supported by:
The Forestry Science and Technology Special Project of the National Forestry and Grassland Administration "Census of Alien Invasive Plant Pathogenic Microorganisms in Xinjiang Forest, Grassland and Wetland Ecosystem"(65000022P00789110010X)

摘要/Abstract

摘要：

【目的】 研究海棠(Malus spp.)上病毒病的种类及田间侵染情况。【方法】 利用宏病毒组测序技术结合RT-PCR方法,对采自新疆7个海棠种植区的158份样品进行病毒检测。【结果】 宏病毒组测序结果只对比到苹果茎痘病毒(Apple stem pitting virus, ASPV)一种植物病毒。158份海棠样品中巴音郭楞蒙古自治州(简称巴州)、阿克苏地区、喀什地区、昌吉回族自治州(简称昌吉州)、石河子市、伊犁哈萨克自治州(简称伊犁州)、奎屯市ASPV检出率分别为0.00%、12.00%、0.00%、31.71%、29.41%、17.65%和0.00%。在检测的7个种植区中,昌吉州检出率最高,为31.71%;石河子市检出率次之,为29.41%;巴州、喀什地区和奎屯市未检测到ASPV。【结论】 海棠上的ASPV在北疆检出率较高,在南疆检出率较低。在观赏海棠样品中检测到ASPV,其病毒检出样本均为田间发病症状叶片,与田间观察到的发病情况相符合,海棠为ASPV的自然寄主之一。

关键词: 海棠; 宏病毒组测序; 苹果茎痘病毒; RT-PCR; 病毒检测

Abstract:

【Objective】 To identify the species and field infection of viral diseases on ornamental Malus spp.【Methods】 A total of 158 samples from 7 areas of Xinjiang were detected by macro virus sequencing technique combined with RT-PCR.【Results】 Only one plant virus was compared with apple stem pitting virus (ASPV).Among 158 samples of ornamental begonia, the ASPV detection rates were 0.00%, 12.00%, 0.00%, 31.71%, 29.41%, 17.65% and 0.00% in Bayingoleng Mongolian Autonomous Prefecture, Aksu Region, Kashgar Region, Changji Hui Autonomous Prefecture, Shihezi City, Yili Kazak Autonomous Prefecture and Kuitun City, respectively.Among the 7 areas tested, Changji had the highest detection rate (31.71%).The detection rate of Shihezi City was 29.41%.ASPV was not detected in Kashgar Prefecture and Kuitun City.【Conclusion】 The results showed that the detection rate of ASPV on ornamental begonia is higher in northern Xinjiang and lower in southern Xinjiang.ASPV is detected in ornamental begonia samples, and the virus detected samples were all symptomatic leaves in the field, which was consistent with the disease observed in the field.Ornamental begonia is one of the natural hosts of ASPV.

Key words: ornamental begonia; macro virus sequencing; apple stem pitting virus; RT-PCR; virus detection

中图分类号:

S436

张李娅, 刘保军, 单佳祁, 王澍, 顾爱星, 白剑宇. 海棠感染苹果茎痘病毒发生与检测[J]. 新疆农业科学, 2024, 61(12): 3061-3066.

ZHANG Liya, LIU Baojun, SHAN Jiaqi, WANG Shu, GU Aixing, BAI Jianyu. Occurrence and detection of begonia infected by apple stem pitting virus[J]. Xinjiang Agricultural Sciences, 2024, 61(12): 3061-3066.

图/表 5

图1 海棠健康及疑似病毒病叶片表型注:A:健康叶片;B、C、D:疑似感病叶片

Fig.1 The Leaves of ornamental begonia phenotypes of healthy and suspected viral disease Note: A: Healthy leaves; B, C, D: suspected susceptible leaves

图2 苹果茎痘病毒特异性引物的PCR检测注:M:DL2000 DNA Marker;1:苹果茎痘病毒;CK:纯净水

Fig.2 Detection of apple stem pitting virus specific primers by PCR Note: M: DL2000 DNA Marker; 1: Apple stem pox virus; CK: Purified water

图3 克隆阳性鉴定PCR检测注:M:DL2000 DNA Marker;1~8:克隆阳性鉴定菌样

Fig.3 Cloning positive identification PCR detection Note:M: DL5000 DNA Marker; 1-8: Clone positive identification of bacterial samples

图4 部分样品苹果茎痘病毒PCR检测结果注:M:DL2000 DNA Marker;1~16:检测样本;CK:纯净水

Fig.4 Results of PCR detection of apple stem poxvirus in some samples Note: M: DL2000 DNA Marker; 1-16: Test sample; CK: Purified water

表1 田间检测结果

Tab.1 Field test results

采样地点 Sampling site	采集样品数 Sample collection	检出样品数 Number of samples detected	检出率 Detection rate(%)
库尔勒 Korla	15	0	0.00
阿克苏 Aksu	25	3	12.00
喀什 Kashgar	7	0	0.00
昌吉 Changji	41	13	31.71
石河子 Shihezi	17	5	29.41
伊犁 Ili	34	6	17.65
奎屯 Kuytun	19	0	0.00
总计 Total	158	27	17.09

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海棠感染苹果茎痘病毒发生与检测

Occurrence and detection of begonia infected by apple stem pitting virus

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