Abstract: 【Objective】 The purpose of this study is to reveal the dynamic changes in the expression of wheat resistance genes under the treatment of Streptomyces sp. HU2014 and to explore the possible mechanism by which this strain induces disease resistance in the host. 【Methods】 In this paper, the cell-free fermentation filtrate of this strain was used to irrigate the roots of three wheat varieties Aikang 58 (AK58), Bainong 307 (BN307), and Zhoumai 22 (ZM22). After irrigation, leaves were collected at 2 h, 6 h, 12 h, 24 h, 48 h, and 96 h, and the changes in the expression of related resistance genes were detected by real-time fluorescent quantitative PCR technology. 【Results】 The fermentation filtrate of this strain could induce the expression of 4 defense response genes, 1 salicylic acid pathway gene, and 1 jasmonic acid pathway gene. Among them, the transcript of TaPR1 gene accumulated at highest levels at 6 h in AK58, 24 h in BN307, and 48 h in ZM22 respectively. The expression of TaPR2 gene reached the highest level at 6 hours in AK58, 12 h in BN307, and 24 h in ZM22 respectively. The transcript of TaPR3 gene reached its peak at 2 h in AK58, 6 h in BN307, and 2 h in ZM22 respectively. TaPR5 gene reached its peak at 72 h in AK58, 96 h in BN307, and 12 h in ZM22 respectively. The PAL gene showed the highest fold change at 48 h in AK58, 24 h in BN307, and 24 h in ZM22 respectively. The expression of LOX gene reached its peak at 6 h in AK58, BN307, and ZM22 respectively. 【Conclusion】 The results indicate that the TaPR1 gene is down-regulated in AK58 and ZM22, and the TaPR3 gene is down-regulated in AK58 and BN307. The highest expression levels of TaPR2, TaPR3, TaPR5, PAL, and LOX in ZM22 are higher than those in AK58 and BN307, while the highest expression level of TaPR1 in BN307 is higher than that in AK58 and ZM22. Streptomyces sp. HU2014 can induce systemic acquired resistance and systemic resistance in wheat.