不同引发剂处理对春小麦种子活力及生理特性的影响

Effects of different priming treatments on seed vigor and physiological characteristics of spring wheat

  • 摘要: 【目的】 研究引发剂对春小麦种子萌发及幼苗生长特性的影响,为增强春小麦种子活力提供理论依据。 【方法】 以春小麦新春26号种子为供试材料,用不同浓度水杨酸SA(0.05、0.1、0.15 mmol/L)、氯化钙CaCl2(6、9、12 mmol/L)、蒸馏水H2O分别引发4、6、8、10、12和14 h后回干,测定春小麦种子发芽势、发芽率、发芽指数、活力指数、幼苗鲜干重、苗长和根长、电导率、SOD活性、POD活性、脱氢酶活性和丙二醛含量等指标。 【结果】 不同引发剂处理春小麦种子发芽势、发芽率、发芽指数、活力指数、幼苗鲜干重、苗长和根长、SOD、POD、脱氢酶活性等指标均较对照显著提高,电导率和丙二醛含量显著降低,其中A2处理和B1处理的效果最优。引发12 h时各引发剂处理的春小麦发芽指标、幼苗生长指标、生理指标均达到最大值,与其他引发时间之间差异显著。 【结论】 引发剂处理使春小麦种子酶活性增强,有效地抵抗了膜质过氧化作用,减少膜质过氧化产物含量,维持细胞结构的完整性,从而有效提高春小麦种子活力,促进幼苗生长,其中0.1 mmol/L SA和6 mmol/L CaCl2引发12 h的效果最佳。

     

    Abstract: 【Objective】 To study the effects of different seed priming on seed germination and seedling growth characteristics of spring wheat in the hope of providing theoretical basis for improving seed vitality of the wheat. 【Methods】 Xinchun 26 wheat seeds were taken as the test material, and primed with different concentrations of SA (0.05, 0.1, 0.15 mmol/L), CaCl2 (6, 9, 12 mmol/L) and water for different time 4, 6, 8, 10, 12 and 14 h, respectively to measure the indexes such as the germination potential, germination rate, germination index, vigor index, fresh dry weight and length of seedling and root, electrical conductivity, SOD activity, POD activity, dehydrogenase activity and MDA content and other factors. 【Results】 The results showed that the seed germination potential, germination rate, germination index, vigor index, fresh dry weight, seedling length and root length, SOD, POD, dehydrogenase activity and other indexes of spring wheat treated with different initiators were significantly increased compared with the control, and the electrical conductivity and malondialdehyde content were significantly decreased, among which A2 and B1 treatments had the best effect. Under the condition of different priming time, germination index, seedling growth index and physiological index of each initiator treatment reached the maximum value at 12 h, and there was significant difference between them and other priming time. 【Conclusion】 Seed priming enhances the enzyme activity of spring wheat seeds, effectively resists membrane peroxidation, reduces the content of membrane peroxidation products, maintains the integrity of cell structure, and effectively improves the seed activity of spring wheat, and promotes the growth of seedlings. Among them, 0.1 mmol/L SA and 6 mmol/L CaCl2 have the best effect on the priming time of 12 h.

     

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