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CRISPR/Cas9 editing and expression analysis of LOC_Os10g05490 in rice under cold stress
SHEN Chunxiu
Xinjiang Agricultural Sciences
2017, 1 (1):
1-10.
DOI: 10.3969/j.issn.1004-1524.2017.01.01
To explore the expression and cold tolerance function of LOC_Os10g05490 in rice under cold stress, semi-quantitative analysis was performed for Dongxiang wild rice and 93-11. The results showed that expression level of LOC_Os10g05490 in 93-11 was the same before and after cold stress, but it was down regulated in Dongxiang wild rice after cold stress. This was consistent with RNA-seq data and further study indicated that this gene might be associated with cold tolerance in Dongxiang wild rice. Plant expression vectors with CRISPR/Cas9 genome editing technique were subsequently constructed to target the LOC_Os10g05490 locus, and were transformed into the japonica rice variety TP309. A total of 26 transgenic positive plants were obtained. This study may lay the foundation for subsequent screening of mutant plants and identification of genes associated with cold tolerance in rice.
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