Comparison of postharvest sugar content and sugar metabolizing enzyme activities of different varieties of thick-skinned melons

doi:10.6048/j.issn.1001-4330.2023.06.021

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (6): 1476-1484.DOI: 10.6048/j.issn.1001-4330.2023.06.021

• Microbes·Horticultural Special Local Products·Storage and Preservation Processing • Previous Articles Next Articles

Comparison of postharvest sugar content and sugar metabolizing enzyme activities of different varieties of thick-skinned melons

JIA Binxin¹^,²(), YANG Shiying¹^,², WANG Yan², YANG Pengpeng³, HE Weizhong², WANG Cheng², LIU Fengjuan²(), FAN Yingying²()

1. College of Food and Pharmaceutical Sciences, Xinjiang Agricultural University, Urumqi 830052, China
2. Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), of Ministry of Agriculture and Rural Affairs of the P.R.C. / Research Institute of Agricultural Quality Standards & Testing Technology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
3. College of Biological and Geographical Sciences, Yili Normal University, Yining Xinjiang 835000, China

Received:2022-10-16 Online:2023-06-20 Published:2023-06-20
Correspondence author: LIU Fengjuan(1985-), female, Xinle, Hebei, associate researcher, PhD, research direction: quality and safety of agricultural products, (E-mail) liufengjuan2050@1236.com; FAN Yingying(1987-), female, Gongyi, Henan, associate researcher, PhD in progress, research direction: quality and safety of agricultural products, (E-mail) fyyxaas@sina.com
Supported by:
Basic Scientific R &D Program Projects of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China(KY2020108);Basic Scientific R &D Program Projects of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China(KY2021139)

不同品种厚皮甜瓜采后糖含量及糖代谢酶活性比较

贾斌鑫¹^,²(), 杨世英¹^,², 王艳², 杨朋朋³, 何伟忠², 王成², 刘峰娟²(), 范盈盈²()

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052
2.新疆农业科学院农业质量标准与检测技术研究所/农业农村部农产品质量安全风险评估实验室(乌鲁木齐)/新疆农产品质量安全重点实验室,乌鲁木齐 830091
3.伊犁师范大学生物与地理科学学院,新疆伊宁 835000

通讯作者: 刘峰娟(1985-),女,河北新乐人,副研究员,博士,研究方向为农产品质量与安全,(E-mail) liufengjuan2050@1236.com; 范盈盈(1987-),女,河南巩义人,副研究员,博士研究生,研究方向为农产品质量与安全,(E-mail) fyyxaas@sina.com
作者简介:贾斌鑫(1996-),女,辽宁朝阳人,硕士研究生,研究方向为食品加工与安全,(E-mail)1071989701@qq.com
基金资助:
自治区公益性科研院所基本科研业务经费项目(KY2020108);自治区公益性科研院所基本科研业务经费项目(KY2021139)

Abstract

Abstract:

【Objective】 To compare the differences in the sugar content and their associated metabolic enzyme activity among different varieties of thick-skinned melons in Xinjiang.【Methods】 9 varieties of thick-skinned melons were tested. The soluble sugar, invertase (Ivr), sucrose phosphate synthase (SPS) and sucrose synthase (SS) were extracted and determined. Meanwhile, the correlation between sugar content and their associated metabolic enzyme activity were analyzed. 【Results】 Results showed that significant differences exist in the contents of fructose, glucose and sucrose among different varieties of melons. Fructose, glucose and sucrose content ranged from 2.17 to 3.21 g/100 g, 1.09 to 2.56 g/100 g and 0.77 to 5.76 g/100 g, respectively. Among which, Huang Meng cui has the highest fructose content, Jun xiu has the highest glucose content, and Zao zui xian has the highest sucrose content. The activities of SPS and SS of "Gan tian mi bao" were the highest, which were 7.5 μmol/(h·g) and 8.7 μmol/(h·g), respectively. Ivr of all the 9 melons have low activity.【Conclusion】 There were different correlations between sugar content and their associated metabolic enzyme activity among different varieties of melons.

Key words: thick-skinned melon; soluble sugar; invertase; sucrose phosphate enzyme; sucrose synthase

摘要：

【目的】研究新疆不同品种甜瓜糖组分及其相关代谢酶活性差异。【方法】以9种新疆特色厚皮甜瓜为试材,提取和测定甜瓜中可溶性糖和转化酶(Ivr)、蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS),分析糖组分和糖代谢相关酶相关性。【结果】不同品种甜瓜的果糖、葡萄糖和蔗糖含量存在差异,果糖、葡萄糖和蔗糖含量范围分别为2.17~3.21、1.09~2.56和0.77~5.76 g/100 g;其中果糖含量最高的是黄梦脆,葡萄糖含量最高的是俊秀,蔗糖含量最高的是早醉仙。甘甜蜜宝的蔗糖磷酸合酶和蔗糖合成酶活性最高,分别为7.5和8.7 μmol/(h·g),9种成熟甜瓜中转化酶处在低活性状态。【结论】不同品种厚皮甜瓜的糖组分和糖代谢相关酶存在着不同相关性。

关键词: 厚皮甜瓜, 可溶性糖, 转化酶, 蔗糖磷酸合酶, 蔗糖合成酶

CLC Number:

S652

JIA Binxin, YANG Shiying, WANG Yan, YANG Pengpeng, HE Weizhong, WANG Cheng, LIU Fengjuan, FAN Yingying. Comparison of postharvest sugar content and sugar metabolizing enzyme activities of different varieties of thick-skinned melons[J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1476-1484.

贾斌鑫, 杨世英, 王艳, 杨朋朋, 何伟忠, 王成, 刘峰娟, 范盈盈. 不同品种厚皮甜瓜采后糖含量及糖代谢酶活性比较[J]. 新疆农业科学, 2023, 60(6): 1476-1484.

Figures/Tables 11

References 23

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	SPS,SS提取缓冲液 Sucrose phosphate synthase, sucrose synthase extraction buffer pH 7.0	SPS,SS脱盐缓冲液 Sucrose phosphate synthase, sucrose synthase desalting buffer pH 7.0	Ivr提取缓冲液 Invertaseextraction buffer pH 7.5	Ivr脱盐缓冲液 Ivertase desalting buffer pH 7.5
反应体系 Reaction System	50 mmol/L Tris-HCl	25 mmol/L Tris-HCl	100 mmol/L 磷酸缓冲液	20 mmol/L 磷酸缓冲液
	5 mmol/L MgCl₂	2.5 mmol/L MgCl₂	5 mmol/L MgCl₂	0.25 mmol/L MgCl₂
	2 mmol/L EDTA-Na₂	1 mmol/L EDTA-Na₂	0.1%琉基乙醇	0.01%琉基乙醇
	2%乙二醇	1%乙二醇	0.05%Triton-X100	0.05%BSA
	0.2%BSA	1 mmol/L DTT	0.05%BSA
	2%PVP		2%PVPP
	5 mmol/L DTT		100 mmol/L 磷酸缓冲液

	SPS,SS提取缓冲液 Sucrose phosphate synthase, sucrose synthase extraction buffer pH 7.0	SPS,SS脱盐缓冲液 Sucrose phosphate synthase, sucrose synthase desalting buffer pH 7.0	Ivr提取缓冲液 Invertaseextraction buffer pH 7.5	Ivr脱盐缓冲液 Ivertase desalting buffer pH 7.5
反应体系 Reaction System	50 mmol/L Tris-HCl	25 mmol/L Tris-HCl	100 mmol/L 磷酸缓冲液	20 mmol/L 磷酸缓冲液
	5 mmol/L MgCl₂	2.5 mmol/L MgCl₂	5 mmol/L MgCl₂	0.25 mmol/L MgCl₂
	2 mmol/L EDTA-Na₂	1 mmol/L EDTA-Na₂	0.1%琉基乙醇	0.01%琉基乙醇
	2%乙二醇	1%乙二醇	0.05%Triton-X100	0.05%BSA
	0.2%BSA	1 mmol/L DTT	0.05%BSA
	2%PVP		2%PVPP
	5 mmol/L DTT		100 mmol/L 磷酸缓冲液

	蔗糖磷酸合成酶 Sucrose phosphate synthase pH 7.0	蔗糖合成酶(合成方向) Sucrose synthase (synthetic direction) pH 7.0	蔗糖合成酶(分解方向) Sucrose synthase (cleavage direction) pH 5.5	酸性转化酶 Acid invertase pH5.5	中性转化酶 Neutral invertase pH 7.5
	50 mmol/L tris-Hcl	50 mmol/L tris-Hcl	80 mmol/L Mes缓冲液	0.000 1 mol/L 磷酸缓冲液	0.000 1 mol/L 磷酸缓冲液
反应体系	10 mmol/L 磷酸-6-果糖	10 mmol果糖	5 mmol/L NaF	10 g/L 蔗糖	10 g/L 蔗糖
Reaction	5 mmol/L MgCl₂	5 mmol/L MgCl₂	100 mmol/L 蔗糖	250 μL脱盐后酶液	250 μL脱盐后酶液
System	5 mmol/L DTT	5 mmol/L DTT	5 mmol/L UDP(脲苷二磷酸)
			100 μL脱盐后酶液

	蔗糖磷酸合成酶 Sucrose phosphate synthase pH 7.0	蔗糖合成酶(合成方向) Sucrose synthase (synthetic direction) pH 7.0	蔗糖合成酶(分解方向) Sucrose synthase (cleavage direction) pH 5.5	酸性转化酶 Acid invertase pH5.5	中性转化酶 Neutral invertase pH 7.5
	50 mmol/L tris-Hcl	50 mmol/L tris-Hcl	80 mmol/L Mes缓冲液	0.000 1 mol/L 磷酸缓冲液	0.000 1 mol/L 磷酸缓冲液
反应体系	10 mmol/L 磷酸-6-果糖	10 mmol果糖	5 mmol/L NaF	10 g/L 蔗糖	10 g/L 蔗糖
Reaction	5 mmol/L MgCl₂	5 mmol/L MgCl₂	100 mmol/L 蔗糖	250 μL脱盐后酶液	250 μL脱盐后酶液
System	5 mmol/L DTT	5 mmol/L DTT	5 mmol/L UDP(脲苷二磷酸)
			100 μL脱盐后酶液

品种 Variety	果糖含量 Fructose content (g/100g)	葡萄糖含量 Glucose content (g/100g)	蔗糖含量 Sucrose content (g/100g)
甘甜蜜宝 Gantianmibao	2.80±0.01^e	2.26±0.02^c	0.77±0.01ⁱ
西州蜜1号 Xizhoumi 1	2.17±0.03^h	1.09±0.02^f	4.39±0.03^c
早醉仙 Zaozuixian	2.57±0.04^g	1.65±0.03^e	5.76±0.02^a
纳斯密 Nasimi	2.91±0.01^d	2.29±0.03^c	3.32±0.02^e
黄梦脆 Huangmengcui	3.21±0.01^a	2.41±0.03^b	2.55±0.05^f
白皮脆 Baipicui	2.70±0.01^f	1.73±0.03^d	2.25±0.02^g
西州蜜25号 Xizhoumi 25	3.15±0.04^b	1.63±0.04^e	5.05±0.05^b
俊秀 Junxiu	2.91±0.01^d	2.56±0.05^a	2.08±0.03^h
20-123	3.01±0.01^c	1.76±0.04^d	4.14±0.04^d

Comparison of postharvest sugar content and sugar metabolizing enzyme activities of different varieties of thick-skinned melons

不同品种厚皮甜瓜采后糖含量及糖代谢酶活性比较

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Figures/Tables 11

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项目 Item	品种 Variety	糖代谢酶 Sugar metabolic enzymes
项目 Item	品种 Variety	AI	NI	SPS	SS(分解)	SS(合成)
蔗糖 Sucrose	甘甜蜜宝	-0.219	1.000^**	0.26	-0.435	0.9
	西州蜜1号	-0.935	0.277	0.363	-0.025	0.625
	早醉仙	0.626	-0.873	-0.908	-0.1	-0.971
	纳斯密	0.33	-0.987	-0.94	-0.961	-0.747
	黄梦脆	0.383	-0.54	0.866	0.159	0.756
	白皮脆	0.78	0.037	-0.992	0.576	0.626
	西州蜜25号	-0.096	0.041	-0.944	0.142	1.000^**
	俊秀	-1.000^*	0.711	-0.515	-0.999^*	0.997^*
	20-123	-0.771	-0.372	-0.044	0.987	-0.999^*

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项目 Item	品种 Variety	糖代谢酶 Sugar metabolic enzymes
项目 Item	品种 Variety	AI	NI	SPS	SS(分解)	SS(合成)
果糖 Fructose	甘甜蜜宝	0.918	0.172	0.997^*	0.804	0.595
	西州蜜1号	0.942	0.410	0.908	.999^*	-0.817
	早醉仙	-0.979	-0.040	-0.96	0.578	-0.89
	纳斯密	0.992	-0.048	0.998^*	0.843	0.905
	黄梦脆	0.128	0.05	-0.997	-0.566	-0.964
	白皮脆	0.245	0.893	-0.943	0.733	0.45
	西州蜜25号	0.21	0.075	-0.875	0.308	0.985
	俊秀	-0.986	0.566	0.996	0.625	-0.644
	20-123	-0.382	-0.778	-0.033	0.989	-0.999^*

项目 Item	品种 Variety	糖代谢酶 Sugar metabolic enzymes
项目 Item	品种 Variety	AI	NI	SPS	SS(分解)	SS(合成)
果糖 Fructose	甘甜蜜宝	0.918	0.172	0.997^*	0.804	0.595
	西州蜜1号	0.942	0.410	0.908	.999^*	-0.817
	早醉仙	-0.979	-0.040	-0.96	0.578	-0.89
	纳斯密	0.992	-0.048	0.998^*	0.843	0.905
	黄梦脆	0.128	0.05	-0.997	-0.566	-0.964
	白皮脆	0.245	0.893	-0.943	0.733	0.45
	西州蜜25号	0.21	0.075	-0.875	0.308	0.985
	俊秀	-0.986	0.566	0.996	0.625	-0.644
	20-123	-0.382	-0.778	-0.033	0.989	-0.999^*

项目 Item	品种 Variety	糖代谢酶 Sugar metabolic enzymes
项目 Item	品种 Variety	AI	NI	SPS	SS(分解)	SS(合成)
葡萄糖 Glucose	甘甜蜜宝	0.689	-0.865	0.273	0.836	-0.549
	西州蜜1号	0.557	0.873	0.479	0.781	-1.000^*
	早醉仙	-0.54	-0.74	-0.475	0.987	-0.3
	纳斯密	0.642	-0.866	0.49	0.908	0.131
	黄梦脆	-0.971	0.998^*	-0.195	-0.884	-0.377
	白皮脆	0.899	0.257	0.518	0.515	-0.966
	西州蜜25号	0.538	0.647	0.963	0.449	-0.822
	俊秀	-0.96	0.867	0.861	0.901	-0.912
	20-123	0.999^*	0.854	-0.929	-0.474	0.369

项目 Item	品种 Variety	糖代谢酶 Sugar metabolic enzymes
项目 Item	品种 Variety	AI	NI	SPS	SS(分解)	SS(合成)
葡萄糖 Glucose	甘甜蜜宝	0.689	-0.865	0.273	0.836	-0.549
	西州蜜1号	0.557	0.873	0.479	0.781	-1.000^*
	早醉仙	-0.54	-0.74	-0.475	0.987	-0.3
	纳斯密	0.642	-0.866	0.49	0.908	0.131
	黄梦脆	-0.971	0.998^*	-0.195	-0.884	-0.377
	白皮脆	0.899	0.257	0.518	0.515	-0.966
	西州蜜25号	0.538	0.647	0.963	0.449	-0.822
	俊秀	-0.96	0.867	0.861	0.901	-0.912
	20-123	0.999^*	0.854	-0.929	-0.474	0.369