白星花金龟对糖醋液9种挥发物的触角电位和行为反应

doi:10.6048/j.issn.1001-4330.2022.02.025

摘要/Abstract

摘要：

【目的】 研究白星花金龟(Protaetia brevitarsis Lewis)对糖醋液的趋性,寻找对白星花金龟具有引诱活性的化合物。【方法】 运用触角电位(electroantennogram,EAG)仪和“Y”型嗅觉仪测定白星花金龟触角电位和行为反应。【结果】 白星花金龟对9种糖醋液挥发物均有一定的EAG反应,雌、雄成虫对戊酸乙酯、2,2,4,6,6-五甲基庚烷和糠醛有较高的EAG反应值,此外,雄成虫还对乳酸乙酯和正己酸有较高的EAG反应值。“Y”型嗅觉仪分析表明,2,2,4,6,6-五甲基庚烷和乳酸乙酯对白星花金龟雌、雄两性成虫均有良好的引诱作用;白星花金龟雌、雄成虫对糠醛和正己酸的选择存在性别差异,糠醛对雌成虫具有较好引诱作用,雄成虫对其无明显趋性,正己酸对雄成虫具有较好引诱作用,雌成虫对其无明显趋性。【结论】 2,2,4,6,6-五甲基庚烷、乳酸乙酯、糠醛和正己酸可作为白星花金龟引诱剂配方的组成成分。

关键词: 白星花金龟; 糖醋液; 挥发物; EAG; “Y”型嗅觉仪

Abstract:

【Objective】 To elucidate the reason why Potosia brevitarsis Lewis is attracted to sugar-vinegar liquid, and to search for compounds with attractant activity to Potosia brevitarsis Lewis. 【Method】 EAG and “Y”-typed olfactometer were used to tested the EAG and behavioral response of Potosia brevitarsis Lewis. 【Result】 Potosia brevitarsis Lewis contained EAG reaction to 9 volatiles of sugar-vinegar liquid, and the female and male adults had higher EAG reaction values to ethyl valerate, 2, 2, 4, 6, 6-pentamethyl-heptane and furfural, the male adults also had higher EAG reaction values to ethyl lactate and hexanoic acid.The analysis of “Y”-typed olfactometer showed that 2, 2, 4, 6, 6- pentamethyl-heptane and ethyl lactate had good attraction to both male and female adults of the Potosia brevitarsis Lewis.There were gender differences in the selection of furfural and hexanoic acid between female and male adults.Furfural had a good attraction to female adults, but male adults had no obvious tendency to them and hexanoic acid had a good attraction to male adults, while female adults had no obvious tropism to them. 【Conclusion】 2,2,4,6,6-pentamethyl-heptane, ethyl lactate, furfural and hexanoic acid can be used as the components of the attractant formula for the Potosia brevitarsis Lewis.

Key words: Potosia brevitarsis Lewis; sugar-vinegar liquid; volatile; electroantennogram (EAG); “Y”-typed olfactometer

中图分类号:

李明杰, 王少山. 白星花金龟对糖醋液9种挥发物的触角电位和行为反应[J]. 新疆农业科学, 2022, 59(2): 466-473.

LI Mingjie, WANG Shaoshan. Study on Electroantennogram and Behavioral Response of Potosia brevitarsis Lewis to 9 Volatiles in Sugar-Vinegar Liquid[J]. Xinjiang Agricultural Sciences, 2022, 59(2): 466-473.

图/表 4

表1 用于测定白星花金龟触角电位反应的标准化合物

Table 1 Standard compounds used to determine the Electroantennogram response of the Potosia brevitarsis Lewis

序号 Code	化合物 Compound	CAS号 CAS number	纯度 Purity (%)	来源 Source
1	戊酸乙酯	539-82-2	≥99.5	Macklin
2	糠醛	98-01-1	≥99.5	Macklin
3	2,2,4,6,6-五甲基庚烷	13475-82-6	≥98	Macklin
4	L-乳酸乙酯	687-47-8	≥98	Sigma
5	乳酸乙酯	97-64-3	≥98	Sigma
6	己酸乙酯	123-66-0	≥99	Sigma
7	丁酸乙酯	105-54-4	≥99.5	Macklin
8	正己酸	142-62-1	≥98	Sigma
9	2,3-丁二醇	513-85-9	≥98	Macklin

图1 白星花金龟雌、雄成虫对不同挥发物的触角电位反应注:图中不同小写字母(大写字母)分别表示雌、雄成虫对不同化合物在P<0.05(P<0.01)水平下差异显著;数字表示各挥发物EAG反应相对值;**表示雌、雄成虫对同种挥发物的EAG反应差异极显著 (T检验)

Fig.1 Electroantennographic responses of female and male adults of the Potosia brevitarsis Lewis to different volatiles Note:The small letters (capital letters) indicate significant differences between female and male adults at the P<0.05(P<0.01) levels; the numbers indicate the relative EAG values of the volatiles; **indicates significant difference in the EAG response between female and male adults to the same volatile (P< 0.01) (T-test)

图2 白星花金龟雌(A)、雄(B)成虫对不同挥发物的行为选择反应注:*、**分别表示处理与对照之间经χ2检验在P<0.05和P<0.01水平差异显著,ns表示χ2测验结果差异不显著

Fig.2 Behavioral selective responses of female (A) and male (B) adults of Potosia brevitarsis Lewis to different volatiles Note: *、** indicate significant difference between the treatment and the control P<0.05 or P<0.01 level by χ2 test, respectively; ns indicate no significant difference in the results of the χ2 test

表2 白星花金龟雌、雄成虫对不同挥发物的行为选择反应率

Table 2 Behavioral selective response rate of female and male adults of Potosia brevitarsis Lewis to different volatiles

挥发物 Volatiles	性别 Sex	反应率 Response rate(%)	选择反应率 Selection response rate(%)	选择系数 Selection coefficient
乳酸乙酯 Ethyl lactate	雌虫	100.00	65.00	0.30
乳酸乙酯 Ethyl lactate	雄虫	100.00	66.67	0.33
正己酸 Hexanoic acid	雌虫	95.00	54.39	0.09
正己酸 Hexanoic acid	雄虫	98.33	64.41	0.29
2,2,4,6,6-五甲基庚烷 2,2,4,6,6-pentamethyl-Heptane	雌虫	95.00	63.16	0.26
2,2,4,6,6-五甲基庚烷 2,2,4,6,6-pentamethyl-Heptane	雄虫	93.33	64.29	0.29
糠醛 Furfural	雌虫	96.67	65.52	0.31
糠醛 Furfural	雄虫	100.00	48.33	-0.03
戊酸乙酯 Ethyl valerate	雌虫	100.00	55.00	0.10
戊酸乙酯 Ethyl valerate	雄虫	91.67	45.46	-0.09

参考文献 34

[1]	索中毅, 白明, 李莎, 等. 中国白星花金龟地理变异的几何形态学分析及其新疆种群的入侵来源推断[J]. 昆虫学报, 2015, 58(4):408-418.
	SUO Zhongyi, BAI Ming, LI Sha, et al. A geometric morphometric analysis of the morphological variations among Chinese populations of Protaetia brevitarsis( Coleoptera: Scarabaeidae ) with an inference of the invading source of its Xinjiang populations.[J]. Acta Entomologica Sinica, 2015, 58(4):408-418.
[2]	郝双红, 李广泽, 张涛, 等. 白星花金龟行为学观察及其信息素的诱虫效果[J]. 中国生物防治学报, 2005, 21(2):124-126.
	HAO Shuanghong, LI Guangze, ZHANG Tao, et al. Behavior of the Scarab Beetle, Potosia brevitarsis and Pheromone Attraction Effects[J]. Chinese Journal of Biological Control, 2005, 21(2):124-126.
[3]	王政, 孟倩倩, 钟国华. 植食性昆虫取食行为过程及机制研究[J]. 环境昆虫学报, 2014, 36(4):612-619.
	WANG Zheng, MENG Qianqian, ZHONG Guohua. Study on the feeding behavior process and mechanism of phytophagous insect[J]. Journal of Environmental Entomology, 2014, 36(4):612-619.
[4]	Knolhoff Lisa M, Heckel David G. Behavioral Assays for Studies of Host Plant Choice and Adaptation in Herbivorous Insects[J]. Annual Review of Entomology, 2014, 59:263-278. DOI PMID
[5]	陆宴辉, 张永军, 吴孔明. 植食性昆虫的寄主选择机理及行为调控策略[J]. 生态学报, 2008,(10):5113-5122.
	LU Yanhui, ZHANG Yongjun, WU Kongming. Host-plant selection mechanisms and behavioural manipulation strategies of phytophagous insects[J]. Acta Ecologica Sinica, 2008, (10):5113-5122.
[6]	杜家纬. 植物-昆虫间的化学通讯及其行为控制[J]. 植物生理学报, 2001,(3):193-200.
	DU Jiawei. Plant-insect Chemical Communication and Its Behavior Control[J]. Acta Plant Physiology, 2001,(3):193-200.
[7]	王攀, 汪海洋, 单俊奇, 等. 草螟科昆虫性信息素组分结构及分布特点[J]. 生态学杂志, 2017,(1):250-259.
	WANG Pan, WANG Haiyang, DAN Junqi, et al. Structure and distribution characteristics of sex pheromone components in Crambidae insects[J]. Chinese Journal of Ecology, 2017,(1):250-259.
[8]	, Kong , Wei A, Wang , Yi , Guo , Yong U. et al. Behavioral effects of different attractants on adult male and female oriental fruit moths, Grapholitamolesta[J]. Pest Management Science, 2020.
[9]	龚建, 陈立, 王少山. 白星花金龟对醇和醛类植物挥发物的触角电位反应[J]. 新疆农业科学, 2017, 54(6):1085-1092.
	GONG Jian, CHEN Li, WANG Shaoshan. Electroantennogram Responses of Female and Male Adults of Potosia brevitasis Lewis to Volatile Alcohols and Aldehydes[J]. Xinjiang Agricultural Sciences, 2017, 54(6):1085-1092.
[10]	李为争, 杨雷, 申小卫, 等. 金龟甲对蓖麻叶挥发物的触角电位和行为反应[J]. 生态学报, 2013, 33(21):6895-6903.
	LI Weizheng, YANG Lei, SHEN Xiaowei, et al. Electroantennographic and behavioural responses of scarab beetles to Ricinus communis leaf volatiles[J]. Acta Ecologica Sinica, 2013, 33(21):6895-6903.
[11]	马艳, 史黎央, 赵艺, 等. 云斑天牛对不同受害状态下山核桃挥发物成分的触角电位和行为反应[J]. 浙江农林大学学报, 2019, 36(3):12-18.
	MA Yan, SHI Liyang, ZHAO Yi, et al. Electroantennogram and behavioral responses of Batocera horsfieldi(Coleoptera: Cerambycidae) to volatiles released from the Caryacathayensis in different damaged states[J]. Journal of Zhejiang A&F University, 2019, 36(3):12-18.
[12]	王瑞笛, 王少山. 白星花金龟成虫对10种寄主挥发物的嗅觉行为反应[J]. 新疆农业科学, 2018, 55(9):1656-1662. DOI
	WANG Ruidi, WANG Shaoshan. Olfactory Behavioral Responses of Potosia brevitarsis Lewis to 10 Kinds of Host Volatiles[J]. Xinjiang Agricultural Sciences, 2018, 55(9):1656-1662.
[13]	林方辉, 童应华. 椰心叶甲对植物挥发化合物的触角电位与行为反应[J]. 森林与环境学报, 2019, 39(2):105-110.
	LIN Fanghui, TONG Yinghua. Electrophysiological and behavioral responses of Brontispa longissima to plant volatile compounds[J]. Journal of Forest and Environment, 2019, 39(2):105-110.
[14]	杨明禄, 熊仁次, 吐合妮木·艾尔肯, 等. 糖醋液对香梨优斑螟和草蛉的诱杀作用[J]. 植物保护, 2011, 37(2):170-173.
	YANG Minglu, XIONG Renci, Tuhenimu Aierken, et al. Trapping effects of the mixture of sugar and acetic acid on moths and lacewings[J]. Plant Protection, 2011, 37(2):170-173.
[15]	王少山, 周天跃, 刘政, 等. 石河子白星花金龟发生为害调查研究[J]. 中国农学通报, 2011, 27(18):288-292.
	WANG Shaoshan, ZHOU Tianyue, LIU Zheng, et al. Investigation on Occurrences and Damages of Postosia brevitarsis lewis in Shihezi[J]. Chinese Agricultural Science Bulletin, 2011, 27(18):288-292.
[16]	高有华, 于江南. 白星花金龟引诱剂的筛选[J]. 新疆农业大学学报, 2011, 34(4):332-334.
	GAO Youhua, YU Jiangnan. Screening of the Attractants for Potosia brevitarsis Lewis[J]. Journal of Xinjiang Agricultural University, 2011, 34(4):332-334.
[17]	宁眺, 樊建庭, 方宇凌, 等. 不同危害状态下寄主萜烯挥发物含量的变化及松墨天牛对其组分的触角电位反应[J]. 昆虫学报, 2006, 49(2):179-188.
	NING Tiao, FAN Jianting, FANG Yuling, et al. Changes in contents of host volatile terpenes under different damaged states and electroantennogram response of Monochamus alternatus Hope to these volatiles[J]. Acta Entomologica Sinica, 2006, 49(2):179-188.
[18]	李雪, 李建一, 曹雅忠, 等. 华北大黑鳃金龟聚集信息素的分离鉴定及其引诱效果[J]. 植物保护学报, 2018, 45(2):257-265.
	LI Xue, LI Jianyi, CAO Yazhong, et al. Identification and bioassay of aggregation pheromone components of northern China scarab beetle Holotrichia oblita[J]. Journal of Plant Protection, 2018, 45(2):257-265.
[19]	Mei Z L, Qian J U, Jing Q M, et al. EAG and behavioral responses of the large black chafer,Holotrichia parallela(Coleoptera:Scarabaeidae) to its sex pheromone[J]. Acta Entomologica Sinica, 2009.
[20]	张月白, 娄永根. 植物与植食性昆虫化学互作研究进展[J]. 应用生态学报, 2020, 31(7):2151-2160.
	ZHANG Yuebai, LOU Yonggen. Research progress in chemical interactions between plants and phytophagous insects[J]. Chinese Journal of Applied Ecology, 2020, 31(7):2151-2160.
[21]	Bruce T.J, Wadhams L.J, Woodcock C.M. Insect host location: a volatile situation[J]. Trends in Plant Science, 2005, 10:269-274. DOI PMID
[22]	黄金水, 何学友, 丁珌, 等. 星天牛蛹的发育及成虫补充营养特性的研究[J]. 林业科学, 2003,(S1):148-152.
	HUANG Jinshui, HE Xueyou, DING Bi, et al. Study on development of pupa and adult supplement nutrient of anoplophorachinensis[J]. Scientia Ailvae Sinicae, 2003,(S1):148-152.
[23]	梁潇予. 云斑天牛对补充营养寄主的选择性研究[D]. 成都:四川农业大学, 2007.
	LIANG Xiaoyu. Preference of Batocera horsfieldi (Hope)for host ofsupplermentary feeding[D]. Chengdu: Sichuan Agricultural University, 2007.
[24]	鲍晓文, 郑峰, 蔡明飞, 等. 补充营养对梨小食心虫成虫生殖及寿命的影响[J]. 西北农林科技大学学报(自然科学版), 2010, 38(8):119-123.
	BAO Xiaowen, ZHENG Feng, CAI Mingfei, et al. Effects of complementary nutrients on adult's reproduction and longevity of oriental fruit moth, Grapholitha molesta Busck[J]. Journal of Northwest A&F University (Natural Science Ed.), 2010, 38(8):119-123.
[25]	太一梅, 朱斌, 何绍华, 等. 杨梅果蝇综合防治技术研究[J]. 植物保护, 2015, 41(1):193-195,200.
	TAI Yimei, ZHU Bin, HE Shaohua, et al. Integrated control techniques of fruit fly(Drosophila melanogaster Meigen)in waxberry[J]. Plant Protection, 2015, 41(1):193-195,200.
[26]	陈光华, 文家富, 王刚云. 糖醋液诱杀果树害虫白星花金龟试验效果[J]. 陕西农业科学, 2007,(6):55-94.
	CHEN Guanghua, WEN Jiafu, WANG Gangyun. Effects of sugar-vinegar liquid on trapping and killing fruit tree pests Potosiabrevitarsis Lewis[J]. Shaanxi Agricultural Sciences, 2007,(6):55-94.
[27]	孙凡, 胡基华, 王广利, 等. 东北大黑鳃金龟嗅感器超微结构[J]. 昆虫学报, 2007,(7):675-681.
	SUN Fan, HU Jihua, WANG Guangli, et al. Ultrastructure of olfactory sensilla of Holotrichia diomphalia Bates (Coleoptera: Melolonthidae)[J]. Acta Entomologica Sinica, 2007,(7):675-681.
[28]	路常宽, 王晓勤. 苹毛丽金龟触角嗅感器超微结构[J]. 昆虫学报, 2009, 52(1):39-45.
	LU Changkuan, WANG Xiaoqin. Ultrastructure of olfactory sensilla on the antenna of Proagopertha lucidula (Coleoptera:Scarabaeidae)[J]. Acta Entomologica Sinica, 2009, 52(1):39-45.
[29]	张晓军, 孙伟, 张健, 等. 鞘翅目昆虫触角感器研究进展[J]. 安徽农业科学, 2013, 41(7):2932-2935.
	ZHANG Xiaojun, SUN Wei, ZHANG Jian, et al. Research Progress of Coleopteran Insect Species Antennal Sensilla[J]. Journal of Anhui Agriculture. 2013, 41(7):2932-2935.
[30]	王瑞笛. 白星花金龟对不同挥发物的行为反应[D]. 石河子:石河子大学, 2019.
	WANG Ruidi. Behavioral responses of Potosiabrevitarsis Lewis to different volatiles[D]. Shihezi: Shehezi University, 2019.
[31]	袁丽芳. 花椒窄吉丁对寄主挥发物化学感受机制的初步研究[D]. 杨凌:西北农林科技大学, 2016.
	YUAN Lifang. The preliminary study on mechanism of odor perception of Agriluszanthoxylumito volatilesfrom Zanthoxylumbungeanum[D]. Yangling: Northwest A&F University, 2016.
[32]	Angelo C, Giovanni B, Giacinto S, et al. Behavioural and electrophysiological responses to overlooked female pheromone components in the olive fruit fly, Bactroceraoleae (Diptera: Tephritidae)[J]. Chemoecology, 2015, 25(3).
[33]	王惠, 郝双红, 王正明. 一种植物源金龟甲聚集素的分离与鉴定[C]. 全国植物性农药研究与开发学术讨论会.中国植物保护学会,西安, 1999:124-127.
	WANG Hui, HAO Shuanghong, WANG Zhengming. Seperationang Identification of a botanic Aggregation pheromone of a Scarab beetle[C]. National Symposium on Research and Development of Plant Pesticides, Chinese Plant Protection Society, Xi'an, 1999:124-127.
[34]	闫凤鸣. 化学生态学[M]: 北京: 科学出版社, 2011.
	YAN Fengming. Chemical ecology [M]. Beijing: Science Press, 2011.