黄地老虎成虫对15种植物挥发物的电生理和行为反应

doi:10.6048/j.issn.1001-4330.2020.11.008

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (11): 2020-2027.DOI: 10.6048/j.issn.1001-4330.2020.11.008

黄地老虎成虫对15种植物挥发物的电生理和行为反应

李琳^1,2, 修春丽^2,3, 路伟¹, 陆宴辉²

1.新疆农业大学农学院,乌鲁木齐 830091;
2.中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京 100193;
3.中国农业科学院茶叶研究所,杭州 310008

收稿日期:2020-03-15 出版日期:2020-11-20 发布日期:2020-10-23
通信作者: 陆宴辉(1980-),男,浙江人,研究员,博士生导师,研究方向为农业昆虫与害虫防治,(E-mail) luyanhui@caas.cn
作者简介:李琳(1994-),女,山东人,硕士研究生,研究方向为农业昆虫与害虫防治,(E-mail)543409281@qq.com
基金资助:
国家重点研发计划项目(2017YFD0201900);国家棉花产业技术体系(CARS-15-19)

Electrophysiological and Behavioral Responses of Agrotis Segetum Adults to 15 Plant Volatiles

LI Lin^1,2, XIU Chunli^2,3, LU Wei¹, LU Yanhui²

1. College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China;
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China

Received:2020-03-15 Online:2020-11-20 Published:2020-10-23
Correspondence author: LU Yanhui (1980-), male, researcher, doctoral tutor, research direction for Agricultural Insects and Pest Control, (E-mail) luyanhui@caas.cn
Supported by:
Supported by the National Key R & D Project (2017YFD0201900); and China Agriculture Research System (CARS-15-19)

摘要/Abstract

摘要： 【目的】 筛选对黄地老虎Agrotis segetum雌性成虫具有引诱活性的植物挥发物,为黄地老虎食诱剂的研发提供候选物质【方法】 利用触角电位仪(EAG)测试黄地老虎雌性成虫对15种植物挥发物的电生理反应,通过风洞试验,评价每种物质对黄地老虎雌性成虫的吸引作用。【结果】 黄地老虎雌性成虫触角对4种挥发物(癸烷、对乙基苯乙酮、2-甲基壬烷和3,3-二甲基辛烷)没有明显的EAG反应,对其余11种挥发物(1,2-二乙苯、1,4-二乙苯、丁酸丁酯、4,8-二甲基-1,3,7-壬三烯、桉叶油醇、乙酸叶醇酯、芳樟醇、β-月桂烯、壬醛、罗勒烯和β-蒎烯)的电生理反应明显,且反应程度随着挥发物浓度的增加而增加,在最大测试浓度100 μg/mL时产生最强烈的电生理反应。在风洞测试中,黄地老虎雌性成虫对1,2-二乙苯、1,4-二乙苯、丁酸丁酯、癸烷、4,8-二甲基-1,3,7-壬三烯、对乙基苯乙酮、桉叶油醇、乙酸叶醇酯、芳樟醇、β-月桂烯、壬醛、3,3-二甲基辛烷、罗勒烯和β-蒎烯14种挥发物表现出明显的行为趋好。【结论】 筛选出对黄地老虎雌性成虫具有电生理和行为吸引活性的植物挥发物组分。

关键词: 黄地老虎; 成虫; 植物挥发物; 电生理反应; 行为选择

Abstract: 【Objective】 The study aims to screen plant volatiles attracting adult females of the cutwormAgrotis Segetum.【Method】 An electroantennogram (EAG) was used to test the electrophysiological response of female A. Segetum adults to 15 kinds of plant volatiles, and meanwhile,the attraction effect of each substance to female A. Segetum adults was evaluated by wind tunnel test.【Result】 Female adults did not show significant electrophysiological responses to four compounds (Decane, 4-Ethylacetophenone, 2-methylnonane and Octane, 3,3-dimethyl-). However, other 11 volatiles including Benzene, 1,2-diethyl-, Benzene, 1,4-diethyl-, Butanoic acid, butyl ester, (3E)-4,8-dimethyl-1,3,7-Nonatriene, Eucalyptol, cis-3-Hexenyl acetate, Linalool, β-Myrcene, Nonanal, Ocimene and β-Pinene evoked significant electrophysiological responses in EAG trials. Moreover, the electrophysiological responses increased with the increasing volatile concentrations, and the strongest response was produced at the maximum test concentration of 100 μg/mL. Female adults were also highly attracted by 14 compounds (Benzene, 1,2-diethyl-, Benzene, 1,4-diethyl-, Butanoic acid, butyl ester, Decane, (3E)-4,8-dimethyl-1,3,7-Nonatriene, 4-Ethylacetophenone, Eucalyptol, cis-3-Hexenyl acetate, Linalool, β-Myrcene, Nonanal, Octane, 3,3-dimethyl-, Ocimene and β-Pinene) in wind tunnel trials.【Conclusion】 Overall, some plant volatiles evoked electrophysiological and behavioral responses by female A. Segetum are screened, which provides candidate substances for the development of its food attractants in the future.

Key words: Agrotis segetum; adult; plant volatile; electrophysiological response; behavioral selection

中图分类号:

S562

李琳, 修春丽, 路伟, 陆宴辉. 黄地老虎成虫对15种植物挥发物的电生理和行为反应[J]. 新疆农业科学, 2020, 57(11): 2020-2027.

LI Lin, XIU Chunli, LU Wei, LU Yanhui. Electrophysiological and Behavioral Responses of Agrotis Segetum Adults to 15 Plant Volatiles[J]. Xinjiang Agricultural Sciences, 2020, 57(11): 2020-2027.

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黄地老虎成虫对15种植物挥发物的电生理和行为反应

Electrophysiological and Behavioral Responses of Agrotis Segetum Adults to 15 Plant Volatiles

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