食品中金黄色葡萄球菌的快速检测及其评估

doi:10.6048/j.issn.1001-4330.2022.02.018

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (2): 410-416.DOI: 10.6048/j.issn.1001-4330.2022.02.018

• 园艺特产·林业·土壤肥料·节水灌溉·农业生态环境 • 上一篇下一篇

食品中金黄色葡萄球菌的快速检测及其评估

谭慧林¹^,²(), 吴忠红³, 金永生⁴, 牛贵洋², 包东东², 陈海元², 张志东¹()

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091
2.新疆阿克苏食品安全检测中心, 新疆阿克苏 843000
3.新疆农业科学院农产品贮藏加工研究所,乌鲁木齐 830091
4.阿克苏地区检验检测中心,新疆阿克苏 843000

收稿日期:2021-01-21 出版日期:2022-02-20 发布日期:2022-03-22
通信作者: 张志东
作者简介:谭慧林(1977-),女,新疆乌鲁木齐人,正高级工程师,研究方向为食品安全检验,(E-mail) 45118707@qq.com
基金资助:
国家重点研发计划(2018YFC1603400);“天山雪松计划”(2017XS26)

Determination of StaphlococcusAureus in Food and Evaluation of the Fast Kit

TAN Huilin¹^,²(), WU Zhonghong³, JIN Yongseng⁴, NIU Guiyang², BAO Dongdong², CHEN Haiyuan², ZHANG Zhidong¹()

1. Xinjiang Laboratory of Special Environmental Microbiology / Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. Food Safety Testing Center of Aksu Prefecture, Aksu Xinjiang 843000, China
3. Research Institute of Agricultural Products Storage and Processing,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China
4. Inspection and Testing Center of Aksu Prefecture, Aksu Xinjiang 843000, China

Received:2021-01-21 Online:2022-02-20 Published:2022-03-22
Correspondence author: ZHANG Zhidong
Supported by:
National Key R & D Project(2018YFC1603400);Tianshan Cedar Program Project(2017XS26)

摘要/Abstract

摘要：

【目的】 分析食品样品中金黄色葡萄球菌的含量,评估金黄色葡萄球菌快检试剂盒的可操作性和准确性。【方法】 分别购买市售苹果脆片和真空包装水果玉米,采用国标GB 4789.10-2006和快检试剂盒测定其金黄色葡萄球菌含量,并以金黄色葡萄球菌为目标菌,采用人工污染样品,根据《食品快速检测方法评价技术规范》评估其快检试剂盒的相关指标。【结果】 苹果脆片和水果玉米中金黄色葡萄球菌含量最高分别为20和50 CFU/g,均低于国标中相关食品中金黄色葡萄球菌限量要求。金黄色葡萄球菌快检试剂盒的灵敏度为93%,特异性为100%、假阴性率6.6%,假阳性率为0,显著性差异为0.5,相对准确度为96.7%,与参比方法检测数据差异不显著。【结论】 金黄色葡萄球菌快检试剂盒检验食品中的金黄色葡萄球菌操作简单,检验结果较为准确,适合基层开展金黄色葡萄球菌的快速筛查。

关键词: 金黄色葡萄球菌; 快速检测; 评估

Abstract:

【Objective】 To analyze content of Staphylococcus aureus in food samples, and evaluate the accuracy and operability of the rapid kit for S.aureus. 【Methods】 Apple chips in bulk and fruit corn in vacuum packaging were sampled, and S.aureus was determined by the method in GB 4789,10-2006 and the rapid kit.S.aureus was taken as the target bacteria, artificial contamination treatments were used, and according to the Technical Specification for Evaluation of Rapid Food Testing Methods, the indexes of the rapid detection kit for S.aureus were evaluated. 【Results】 The highest content of S.aureus in samples of apple chips and fruit corn was 20 and 45 CFU/g, respectively, which were lower than the limit of pathogens in related foods.The sensitivity, specificity, false-negative rate, false-positive rate and relative accuracy of the kit were 93%, 100%, 6.6%, 0, and 96.7%, respectively.Significant difference was not found between the rapid kit and the reference method. 【Conclusion】 The rapid kit is easy to operate and detect S.aureus accurately, which is suitable for fast checks.

Key words: Staphylococcus aureus; rapid detection; evaluation

中图分类号:

谭慧林, 吴忠红, 金永生, 牛贵洋, 包东东, 陈海元, 张志东. 食品中金黄色葡萄球菌的快速检测及其评估[J]. 新疆农业科学, 2022, 59(2): 410-416.

TAN Huilin, WU Zhonghong, JIN Yongseng, NIU Guiyang, BAO Dongdong, CHEN Haiyuan, ZHANG Zhidong. Determination of StaphlococcusAureus in Food and Evaluation of the Fast Kit[J]. Xinjiang Agricultural Sciences, 2022, 59(2): 410-416.

图/表 6

参考文献 24

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样品情况^a Sample	检测结果^bResult		总数 Total
样品情况^a Sample	阳性Positive	阴性Negative	总数 Total
阳性Positive	N₁₁	N₁₂	N₁.=N₁₁+N₁₂
阴性Negative	N₂₁	N₂₂	N₂.=N₂₁+N₂₂
总数Total	N.1=N₁₁+N₂₁	N.2=N₁₂+N₂₂	N=N₁.+N₂.
显著性差异($\chi^{2}$)Significant difference($\chi^{2}$)	$\chi^{2}$=(\|N₁₂-N₂₁\|-1)²/(N₁₂+N₂₁)		自由度(df)=1
灵敏度Sensitivity(p+,%)	p+=N₁₁/N₁.
特异性Specificity(p-,%)	p-=N₂₂/N₂.
假阴性率False negative rate(pf-,%	pf-=N₁₂/N₁.=100-灵敏度
假阳性率False negative rate(pf+,%)	pf+=N₂₁/N₂.=100-特异性
相对准确度Relative accuracy(%^c)	(N₁₁+N₂₂)/(N₁.+N₂.)

样品情况^a Sample	检测结果^bResult		总数 Total
样品情况^a Sample	阳性Positive	阴性Negative	总数 Total
阳性Positive	N₁₁	N₁₂	N₁.=N₁₁+N₁₂
阴性Negative	N₂₁	N₂₂	N₂.=N₂₁+N₂₂
总数Total	N.1=N₁₁+N₂₁	N.2=N₁₂+N₂₂	N=N₁.+N₂.
显著性差异($\chi^{2}$)Significant difference($\chi^{2}$)	$\chi^{2}$=(\|N₁₂-N₂₁\|-1)²/(N₁₂+N₂₁)		自由度(df)=1
灵敏度Sensitivity(p+,%)	p+=N₁₁/N₁.
特异性Specificity(p-,%)	p-=N₂₂/N₂.
假阴性率False negative rate(pf-,%	pf-=N₁₂/N₁.=100-灵敏度
假阳性率False negative rate(pf+,%)	pf+=N₂₁/N₂.=100-特异性
相对准确度Relative accuracy(%^c)	(N₁₁+N₂₂)/(N₁.+N₂.)

样品 Sample	计数Accounting						统计 Statistics (CFU/g)	回收率 Rate of recovery (%)
样品 Sample	1∶10			1∶100			统计 Statistics (CFU/g)	回收率 Rate of recovery (%)
苹果脆片 (加标100 CFU/g) Apple chips (artificial pollution treatments, 100 CFU/g )	9	9	11	0	0	0	93	93.3
	8	10	9	0	0	0	93	93.3
苹果脆片 (加标1 000 CFU/g) Apple chips (artificial pollution treatments, 1,000 CFU/g )	97	96	108	10	7	8	1 053	102.5
	107	102	105	9	8	9	1 053	102.5

样品 Sample	计数Accounting						统计 Statistics (CFU/g)	回收率 Rate of recovery (%)
样品 Sample	1∶10			1∶100			统计 Statistics (CFU/g)	回收率 Rate of recovery (%)
苹果脆片 (加标100 CFU/g) Apple chips (artificial pollution treatments, 100 CFU/g )	9	9	11	0	0	0	93	93.3
	8	10	9	0	0	0	93	93.3
苹果脆片 (加标1 000 CFU/g) Apple chips (artificial pollution treatments, 1,000 CFU/g )	97	96	108	10	7	8	1 053	102.5
	107	102	105	9	8	9	1 053	102.5

	T₁	T₂	T₃	T₄	T₅
国标法检测结果 Results by the GB method (CFU/g)	26	86	123	862	1 053
测试片法检测结果 Results by the rapid kit method (CFU/g)	20	80	120	810	1 140
	20	90	110	910	1 180
	20	80	100	870	1 100
	30	80	110	860	1 020
	30	80	120	880	1 040
	20	90	120	910	1 030
	20	80	110	820	1 120
	20	90	110	840	1 030
	30	90	110	900	1 150
	30	80	100	810	1 010

食品中金黄色葡萄球菌的快速检测及其评估

Determination of StaphlococcusAureus in Food and Evaluation of the Fast Kit

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图/表 6

参考文献 24

相关文章 15

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Metrics

样品实际状态^a Sample	测试片检测结果^b Results		合计Total
样品实际状态^a Sample	阳性Postive	阴性 Negative	合计Total
阳性Postive(>100 CFU/g)	28	2	30
阴性Negative(≤100 CFU/g)	0	30	30
合计	30	30	60
评估指标Evaluation indexs
灵敏度 Sensitivity	特异性 Specificity	假阴性率 False negative rate	假阳性率 False negative rate
93%	100%	6.6%	0%

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