不同基因编辑类型对FGF5基因编辑羊羊毛性状的影响

doi:10.6048/j.issn.1001-4330.2024.03.024

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (3): 734-741.DOI: 10.6048/j.issn.1001-4330.2024.03.024

不同基因编辑类型对FGF5基因编辑羊羊毛性状的影响

岳成广¹(), 李忠慧¹^,², 刘晨曦¹^,², 贺三刚¹^,², 马海叶³, 刘璇³, 李婧平¹, 李文蓉¹^,²()

1.新疆畜牧科学院生物技术研究所,乌鲁木齐 830011
2.新疆维吾尔自治区动物生物技术重点实验室,乌鲁木齐 830011
3.新疆农业大学动物科学学院,乌鲁木齐 830052

收稿日期:2023-07-09 出版日期:2024-03-20 发布日期:2024-04-19
通信作者: 李文蓉(1969-),女,江苏人,研究员,博士,硕士生/博士生导师,研究方向为动物遗传育种,(E-mail)xjlwr@126.com
作者简介:岳成广(1997-),男,河南人,硕士,研究方向为动物遗传育种,(E-mail)945372465@qq.com
基金资助:
国家转基因生物新品种培育重大专项子课题“长毛型转基因超细毛羊的培育”(2016ZX08008001-002)

Effects of different gene editing types on traits of FGF5 gene-edited sheep wool

YUE Chenggung¹(), LI Zhonghui¹^,², LIU Chenxi¹^,², HE Sangang¹^,², MA Haiye³, LIU Xuan³, LI Jingping¹, LI Wenrong¹^,²()

1. Institute of Biotechnology,Xinjiang Academy of Animal Sciences,Urumqi 830011,China
2. Key Laboratory of Animal Biotechnology of Xinjiang,Urumqi 830011,China
3. College of Animal Sciences,Xinjiang Agricultural University,Urumqi 830052,China

Received:2023-07-09 Online:2024-03-20 Published:2024-04-19
Correspondence author: LI Wenrong(1969 -),female,from Jiangsu, researcher,Ph.D, doctoral supervisor,majoring in animal genetics and breeding,(E-mail) xjlwr@126.com
Supported by:
National Major Special Sub project for the Cultivation of New Genetically Modified Biological Varieties "Cultivation of Long Hair Type Genetically Modified Superfine Wool Sheep"(2016ZX08008001-002)

摘要/Abstract

摘要：

【目的】 分析不同基因编辑类型对成纤维生长因子5 (FGF5)基因编辑细毛羊羊毛性状的影响,为开展基因编辑细毛羊的羊毛性状遗传参数评估提供理论依据。【方法】 收集181只FGF5周岁基因羊,分析编辑细毛羊羊毛自然长度、伸直长度、羊毛纤维直径和剪毛量等性状的数据。【结果】 FGF5基因编辑细毛羊羊毛自然长度平均值为10.49 cm,变异系数为11.82%。FGF5-InDel和FGF5-HDR基因编辑细毛羊羊毛自然长度和剪毛量均极显著高于野生型(P<0.01)。-28 bp或-26 bp、2 bp和置换(c.61 C>T)的羊毛自然长度和伸直长度均极显著长于wt(P<0.01),-28 bp或-26 bp的剪毛量显著高于wt(P<0.05)。-28 bp基因型的双等位基因编辑和单等位基因编辑突变的羊毛自然长度和伸直长度均极显著长于野生型(P<0.01)。【结论】 不同基因编辑类型的FGF5基因编辑细毛羊均可显著提高羊毛自然长度和剪毛量,可利用表型性状突出的后代组建核心编辑羊群体,促进FGF5基因编辑细毛羊选育扩繁。

关键词: FGF5基因编辑细毛羊; 编辑类型; 羊毛自然长度; 剪毛量; 基因型; 突变类型

Abstract:

【Objective】 To study the effects of different gene editing types on wool traits in FGF5 gene-edited fine-wool sheep in the hope of providing a theoretical basis for subsequent studies on the genetic parameters of wool traits in gene-edited fine-wool sheep.【Methods】 In this study,data on traits such as wool length,wool straight length,wool fiber diameter and greasy fleece weight from 181 FGF5-year-old gene-edited fine-wood sheep from the sheep breeding base of Xinjiang Academy of Animal Sciences were collected for analysis.【Results】 The wool length of FGF5 gene editing fine wool sheep was 10.49 cm with a coefficient variation of 11.82%.Wool length and greasy fleece weight of FGF5-InDel and FGF5-HDR gene-edited fine-wool were extremely significant higher than that of the wild type(P<0.01).The wool length and wool straight length of -28 bp or -26 bp,-2 bp and replacement(c.61 C>T) were extremely significant longer than that of the wild type(P<0.01),and the greasy fleece weight at -28 bp or -26 bp was significantly higher than that of the wild type(P<0.05).The wool length and wool straight length of double-allele-edited and single-allele-edited mutations of the -28 bp genotype had extremely significantly longer than those of thewild type(P<0.01).【Conclusion】 Different gene editing types of FGF5 gene editing in fine wool sheep significantly increase the wool length and greasy fleece weight,which can be used to form a core population of gene-edited sheep with outstanding phenotypic traits,promote the selection and expansion of FGF5 gene-edited sheep.

Key words: FGF5 gene-edited fine-wool sheep; edit type; wool length; greasy fleece weight; genotype; mutation type

中图分类号:

S826
S814.8

岳成广, 李忠慧, 刘晨曦, 贺三刚, 马海叶, 刘璇, 李婧平, 李文蓉. 不同基因编辑类型对FGF5基因编辑羊羊毛性状的影响[J]. 新疆农业科学, 2024, 61(3): 734-741.

YUE Chenggung, LI Zhonghui, LIU Chenxi, HE Sangang, MA Haiye, LIU Xuan, LI Jingping, LI Wenrong. Effects of different gene editing types on traits of FGF5 gene-edited sheep wool[J]. Xinjiang Agricultural Sciences, 2024, 61(3): 734-741.

图/表 6

参考文献 25

[1]	李忠慧. CRISPR/cas9基因编辑技术在羊毛品质改良中的应用[J]. 饲料博览, 2020,(11):32-34.
	LI Zhonghui. Application of CRISPR/cas9 gene editing technology in wool quality improvement[J]. Feed Review, 2020,(11):32-34.
[2]	Zhao H Y, Hu R X, Li F D, et al. Five SNPs Within the FGF5 Gene Significantly Affect Both Wool Traits and Growth Performance in Fine-Wool Sheep(Ovis aries)[J]. Front Genet, 2021, 12:732097. DOI URL
[3]	徐鑫, 刘明军. CRISPR/Cas9基因编辑技术在绵羊中的应用研究进展[J]. 中国畜牧兽医, 2022, 49(11):4129-4138. DOI
	XU Xin, LIU Mingjun. Research progress on application of CRISPR/Cas9 genome editing systems in sheep[J]. China Animal Husbandry & Veterinary Medicine, 2022, 49(11):4129-4138.
[4]	史玉洁, 李芳, 王昕. CRISPR技术应用于山羊和绵羊育种的研究进展[J]. 中国畜牧杂志, 2022, 58(4):16-21.
	SHI Yujie, LI Fang, WANG Xin. Research progress on application of CRISPR in goat and sheep breeding[J]. Chinese Journal of Animal Science, 2022, 58(4):16-21.
[5]	曹俊霞, 王友亮, 王征旭. 精准调控CRISPR/Cas9基因编辑技术研究进展[J]. 遗传, 2020, 42(12):1168-1177.
	CAO Junxia, WANG Youliang, WANG Zhengxu. Advances in precise regulation of CRISPR/Cas9 gene editing technology[J]. Hereditas (Beijing), 2020, 42(12):1168-1177.
[6]	王欢, 邹惠影, 朱化彬, 等. CRISPR/Cas9基因编辑技术在家畜育种新材料创制中的研究进展[J]. 畜牧兽医学报, 2021, 52(4):851-861.
	WANG Huan, ZOU Huiying, ZHU Huabin, et al. Advances in evaluation of livestock breeding new materials by using the CRISPR/Cas9 gene editing technology[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4):851-861.
[7]	Fan Z Q, Perisse I V, Cotton C U, et al. A sheep model of cystic fibrosis generated by CRISPR/Cas9 disruption of the CFTR gene[J]. JCI Insight, 2018, 3(19):e123529. DOI URL
[8]	Yoshizawa Y, Wada K, Shiomi G, et al. A 1-bp deletion in Fgf5 causes male-dominant long hair in the Syrian hamster[J]. Mammalian Genome, 2015, 26(11):630-637. DOI URL
[9]	Mizuno S, Iijima S, Okano T, et al. Retrotransposon-mediated Fgf5(go-Utr) mutant mice with long pelage hair[J]. Experimental Animals, 2011, 60(2):161-167. PMID
[10]	Ota Y, Saitoh Y, Suzuki S, et al. Fibroblast Growth Factor 5 Inhibits Hair Growth by Blocking Dermal Papilla Cell Activation[J]. Biochemical and Biophysical Research Communications, 2002, 290(1):169-176. DOI URL
[11]	Rosenquist T A, Martin G R. Fibroblast growth factor signalling in the hair growth cycle:expression of the fibroblast growth factor receptor and ligand genes in the murine hair follicle[J]. Developmental Dynamics:an Official Publication of the American Association of Anatomists, 1996, 205(4):379-386. PMID
[12]	Li W R, Liu C X, Zhang X M, et al. CRISPR/Cas9-mediated loss of FGF5 function increases wool staple length in sheep[J]. The FEBS Journal, 2017, 284(17):2764-2773. DOI URL
[13]	Ito C, Saitoh Y, Fujita Y, et al. Decapeptide with fibroblast growth factor(FGF)-5 partial sequence inhibits hair growth suppressing activity of FGF-5[J]. Journal of Cellular Physiology, 2003, 197(2):272-283. DOI URL
[14]	He X L, Chao Y, Zhou G X, et al. Fibroblast growth factor 5-short(FGF5s) inhibits the activity of FGF5 in primary and secondary hair follicle dermal papilla cells of Cashmere goats[J]. Gene, 2016, 575(2):393-398. DOI URL
[15]	Zhang L H, He S G, Liu M J, et al. Molecular cloning,characterization,and expression of sheep FGF5 gene[J]. Gene, 2015, 555(2):95-100. DOI URL
[16]	高原, 阿力玛, 李璐, 等. 靶除内蒙古白绒山羊FGF5基因对其毛被性状的影响[J]. 内蒙古农业大学学报(自然科学版), 2016, 37(1):61-65.
	GAO Yuan, A Lima, LI Lu, et al. Effecs of knockout FGF5 in Inner Mongolian white Cashmere goats on fleece traits[J]. Journal of Inner Mongolia Agricultural University(Natural Science Edition), 2016, 37(1):61-65.
[17]	Hu R, Fan Z Y, Wang B Y, et al. RAPID COMMUNICATION:Generation of FGF5 knockout sheep via the CRISPR/Cas9 system[J]. J Anim Sci, 2017, 95(5):2019-2024. DOI PMID
[18]	毛林军. FGF5基因编辑绵羊精液冷冻及体外胚胎的制备与分析[D]. 乌鲁木齐: 新疆农业大学, 2022.
	MAO Linjun. Preparation and analysis of frozen semen and in vitro embryos of FGF5 gene-edited sheep[D]. Urumqi: Xinjiang Agricultural University, 2022.
[19]	胡慧宇. FGF5基因编辑细毛羊的遗传稳定性和自身健康评估[D]. 乌鲁木齐: 新疆农业大学, 2021.
	HU Huiyu. Evaluation of genetic stability and self-health safety by FGF5 gene edited fine-wool sheep[D]. Urumqi: Xinjiang Agricultural University, 2021.
[20]	关鸣轩, 魏趁, 佀博学, 等. 苏博美利奴羊主要经济性状的遗传参数估计[J]. 中国畜牧杂志, 2022, 58(1):97-101.
	GUAN Mingxuan, WEI Chen, SI Boxue, et al. Estimation of genetic parameters of main economic traits of Subo Merino sheep[J]. Chinese Journal of Animal Science, 2022, 58(1):97-101.
[21]	魏趁, 关鸣轩, 付雪峰, 等. 运用贝叶斯方法估计中国美利奴羊(新疆型)毛用性状及繁殖性状的遗传参数[J]. 畜牧兽医学报, 2020, 51(7):1537-1547.
	WEI Chen, GUAN Mingxuan, FU Xuefeng, et al. Estimates of genetic parameters for wool and reproductive traits in Chinese Merino sheep(Xinjiang type) by bayesian method[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7):1537-1547.
[22]	乔国艳, 袁超, 李文辉, 等. 高山美利奴羊重要经济性状遗传参数估计[J]. 中国畜牧杂志, 2019, 55(10):58-62.
	QIAO Guoyan, YUAN Chao, LI Wenhui, et al. Estimation of genetic parameter for important economic traits of Alpine Merino sheep[J]. Chinese Journal of Animal Science, 2019, 55(10):58-62.
[23]	Wang X, Niu Y, Zhou J, et al. CRISPR/Cas9-mediated MSTN disruption and heritable mutagenesis in goats causes increased body mass[J]. Animal Genetics, 2018, 49(1):43-51. DOI PMID
[24]	尚利青, 宋绍征, 张婷, 等. MSTN基因突变纯合子兔的繁育和表型分析[J]. 生物工程学报, 2022, 38(5):1847-1858.
	SHANG Liqing, SONG Shaozheng, ZHANG Ting, et al. Propagation and phenotypic analysis of mutant rabbits with MSTN homozygous mutation[J]. Chinese Journal of Biotechnology, 2022, 38(5):1847-1858 DOI PMID
[25]	Xu Y X, Liu H M, Pan H L, et al. CRISPR/Cas9-mediated Disruption of Fibroblast Growth Factor 5 in Rabbits Results in a Systemic Long Hair Phenotype by Prolonging Anagen[J]. Genes, 2020, 11(3):297. DOI URL

编辑方式 Edit Mode	编辑序列 Edit Sequence	基因型 Genotype	突变类型 Mutation Type
FGF5-InDel	GAGAAGCGCCTCGCACCCAAAGGGCAGCCCGGACCGGCTGCCACCGAGAG GAACCC	WT	FGF5+/FGF5⁺
	GAGAAGCGCCTCGCACC------------GAGAGGAACCC	-28 bp/-28 bp	FGF5-/FGF5^-
	GAGAAGCGCCTCGCACC----------------GAGAGGAACCC GAGAAGCGCCTCGCACCCAAAGGt-----GCCCGGACCGGCTGCCACCGAGAGGAACCC	-28 bp/-2 bp	FGF5-/FGF5^-
	GAGAAGCGCCTCGCACC---------------------GAGAGGAACCC GAGAAGCGCCTCGCACCT--------------------CGAGAGGAACCC	-28 bp/-26 bp	FGF5- /FGF5^-
FGF5-HDR	AGCCACCTGATCCTCAGCGCCTGGGCTCAAGGGGAGAAGCGCCTCGCAC	wt	FGF5+/FGF5⁺
FGF5-HDR	AGCCACCTGATCCTCAGCGCCTGGGCTTAAGGGGAGAAGCGCCTCGCACC	置换	FGF5-/FGF5-

编辑方式 Edit Mode	编辑序列 Edit Sequence	基因型 Genotype	突变类型 Mutation Type
FGF5-InDel	GAGAAGCGCCTCGCACCCAAAGGGCAGCCCGGACCGGCTGCCACCGAGAG GAACCC	WT	FGF5+/FGF5⁺
	GAGAAGCGCCTCGCACC------------GAGAGGAACCC	-28 bp/-28 bp	FGF5-/FGF5^-
	GAGAAGCGCCTCGCACC----------------GAGAGGAACCC GAGAAGCGCCTCGCACCCAAAGGt-----GCCCGGACCGGCTGCCACCGAGAGGAACCC	-28 bp/-2 bp	FGF5-/FGF5^-
	GAGAAGCGCCTCGCACC---------------------GAGAGGAACCC GAGAAGCGCCTCGCACCT--------------------CGAGAGGAACCC	-28 bp/-26 bp	FGF5- /FGF5^-
FGF5-HDR	AGCCACCTGATCCTCAGCGCCTGGGCTCAAGGGGAGAAGCGCCTCGCAC	wt	FGF5+/FGF5⁺
FGF5-HDR	AGCCACCTGATCCTCAGCGCCTGGGCTTAAGGGGAGAAGCGCCTCGCACC	置换	FGF5-/FGF5-

编辑方式 Edit mode	家系 (Family)	F₀父本(基因型) F₀ sire (Genotype)	F₀代选配类型 F₀ type	F₁代羔羊 (基因型) F₁ lambs (Genotype)	F₁父本 (基因型) F₁ sire (Genotype)	F₁代选配类型 F₁ type	F₂代羔羊 (基因型) F₂ lambs (Genotype)
FGF5-InDel	1	GM007 (-28 bp/-2 bp、G>T, FGF5^-/FGF5^-)	F₀♂×WT♀ F₀♂×F₀♀	-28 bp,杂合 -2 bp杂合	GM18090 (-28 bp,杂合)	F₀♂×WT♀	-28 bp,杂合 WT/WT
	2	GM009 (-28 bp/-28 bp, FGF5^-/FGF5^-)	F₀♂×WT♀	-28 bp,杂合	TG127 (-28 bp,杂合)	F₀♂×WT♀ F₀♂×F₀♀	-28 bp,杂合 WT/WT 其他
	3	GM022 (-28 bp/-26 bp, FGF5^-/FGF5^-)	F₀♂×WT♀	-28 bp,杂合 -26 bp,杂合	\	\	\
FGF5-HDR	4	GM18042 (置换, FGF5^-/FGF5^-)	F₀♂×WT♀ F₀♂×F₀♀	C>T,杂合	\	\	\

编辑方式 Edit mode	家系 (Family)	F₀父本(基因型) F₀ sire (Genotype)	F₀代选配类型 F₀ type	F₁代羔羊 (基因型) F₁ lambs (Genotype)	F₁父本 (基因型) F₁ sire (Genotype)	F₁代选配类型 F₁ type	F₂代羔羊 (基因型) F₂ lambs (Genotype)
FGF5-InDel	1	GM007 (-28 bp/-2 bp、G>T, FGF5^-/FGF5^-)	F₀♂×WT♀ F₀♂×F₀♀	-28 bp,杂合 -2 bp杂合	GM18090 (-28 bp,杂合)	F₀♂×WT♀	-28 bp,杂合 WT/WT
	2	GM009 (-28 bp/-28 bp, FGF5^-/FGF5^-)	F₀♂×WT♀	-28 bp,杂合	TG127 (-28 bp,杂合)	F₀♂×WT♀ F₀♂×F₀♀	-28 bp,杂合 WT/WT 其他
	3	GM022 (-28 bp/-26 bp, FGF5^-/FGF5^-)	F₀♂×WT♀	-28 bp,杂合 -26 bp,杂合	\	\	\
FGF5-HDR	4	GM18042 (置换, FGF5^-/FGF5^-)	F₀♂×WT♀ F₀♂×F₀♀	C>T,杂合	\	\	\

性状 Trait	记录数 Recode	最小值 Minimum	最大值 Maximum	平均值 Average	标准差 Standard deviation	变异系数 Coefficient of variation
羊毛自然长度Wool length(cm)	180	7.00	15.50	10.49	1.24	11.82
伸直长度Wool straight length(cm)	178	8.24	17.16	12.69	1.61	12.69
羊毛纤维直径Wool fiber diameter(μm)	181	14.80	27.84	18.97	2.02	10.65
剪毛量Greasy fleece weight(kg)	166	1.90	8.00	4.51	1.19	26.21

不同基因编辑类型对FGF5基因编辑羊羊毛性状的影响

Effects of different gene editing types on traits of FGF5 gene-edited sheep wool

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图/表 6

参考文献 25

相关文章 15

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Metrics

性状Trait	编辑方式Edit Mode
性状Trait	FGF5-InDel	FGF5-HDR	WT
羊毛自然长度Wool length(cm)	10.44±1.31^A(147)	10.74±0.81^A(33)	9.67±1.00^B(29)
伸直长度Wool straight length(cm)	12.57±1.62^a(145)	13.23±1.43b^B(33)	11.04±1.09^cC(29)
羊毛纤维直径Wool fiber diameter(μm)	19.07±1.98(148)	18.51±2.15(33)	18.38±2.24(29)
剪毛量Greasy fleece weight(kg)	4.43±1.27^A(133)	4.86±0.74^A(33)	3.82±1.16^B(29)

性状 Trait	基因型Genotype
性状 Trait	-28 bp或-26 bp	-2 bp	替换(C>T,61)	WT
羊毛自然长度Wool length(cm)	10.50±1.22^A(89)	10.57±1.50^A(30)	10.74±0.81^A(33)	9.67±1.00^B(29)
伸直长度Wool straight length(cm)	12.73±1.55^A(89)	12.51±1.91^A(30)	13.29±1.43^A(33)	11.04±1.09^B(29)
羊毛纤维直径Wool fiber diameter(μm)	19.21±2.10(91)	18.67±1.22(30)	18.51±2.15(33)	18.38±2.24(29)
剪毛量Greasy fleece weight(kg)	4.39±1.28^a(86)	4.75±1.34^aB(29)	4.86±0.74^aB(33)	3.82±1.16^bC(29)

性状 Trait	突变类型Mutation type
性状 Trait	-/-	-/+	+/+
羊毛自然长度Wool length(cm)	10.50±1.54^A(12)	10.50±1.17^A(77)	9.67±1.00^B(29)
伸直长度Wool straight length(cm)	12.21±1.83^A(12)	12.81±1.50^A(77)	11.04±1.09^B(29)
羊毛纤维直径Wool fiber diameter(μm)	18.97±1.90(13)	19.25±2.14(78)	18.38±2.24(29)
剪毛量Greasy fleece weight(kg)	3.97±1.49^ab(12)	4.47±1.24^a(71)	3.82±1.16^b(29)

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[5]	刘晓婷,罗燕,陈洁,连科讯,刘钢,马昭,朱晓庆,谷新利. 中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡育雏育成期免疫功能的影响[J]. 新疆农业科学, 2018, 55(4): 763-773.
[6]	张校立,徐叶挺,艾沙江·买买提,许娟,邓莉,王继勋. 新疆地方梨品种S基因型的鉴定[J]. 新疆农业科学, 2018, 55(2): 246-252.
[7]	王重;樊哲儒;张跃强;李剑峰;高新;王子霞. 春小麦单倍体胚得胚率的影响因素研究[J]. , 2016, 53(8): 1404-1408.
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[10]	曾斌;高启明;田嘉;李疆. 新疆扁桃品种自交不亲和S-Rnases基因型的分析鉴定[J]. , 2014, 51(8): 1400-1408.
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[12]	李智军;刘武军;祁居中;孟军;刘佳;耿明;姚新奎. 利用微卫星多态性鉴别不同马品种试验[J]. , 2013, 50(9): 1692-1703.
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[14]	甘尚权;张伟;沈敏;李欢;梁耀伟;杨井泉;高磊;刘守仁;王新华. 绵羊X染色体59578440位点多态分析及其与尾(臀)脂性状相关性研究[J]. , 2013, 50(12): 2311-2316.
[15]	孟灵真;陈全家;杨婷;阿依夏木·古丽;王希东;曲延英. 农杆菌介导的bar基因和Bt基因的遗传转化研究[J]. , 2013, 50(12): 2189-2196.