赛里木酸奶中乳酸菌的分离鉴定及特性分析

doi:10.6048/j.issn.1001-4330.2023.09.029

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (9): 2323-2330.DOI: 10.6048/j.issn.1001-4330.2023.09.029

• 草业·畜牧兽医·食品工艺技术 • 上一篇下一篇

赛里木酸奶中乳酸菌的分离鉴定及特性分析

何齐¹(), 冯倩¹, 李雪¹, 易鸳鸯², 顾美英², 朱静², 孙建², 张志东¹^,²()

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052
2.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091

收稿日期:2022-12-26 出版日期:2023-09-20 发布日期:2023-09-19
通信作者: 张志东(1977-),男,新疆乌鲁木齐人,研究员,博士,硕士生导师,研究方向为特殊环境微生物及益生菌资源挖掘,(E-mail)zhangzheedong@qq.com
作者简介:何齐(1997-),男,安徽人,硕士研究生,研究方向为食品科学与食品安全, (E-mail)heqi9711@163.com
基金资助:
新疆区域协同创新专项(上海合作组织科技伙伴计划)(2020E01007);国家重点研发计划项目(2018YFC1603400);国家重点研发计划项目(2018YFC1603404)

Screening, identification and characteristics of Lactic acid bacteria from the sayram ketteki

HE Qi¹(), FENG Qian¹, LI Xue¹, YI Yuanyang², GU Meiying², ZHU Jin², SUN Jian², ZHANG Zhidong¹^,²()

1. College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China
2. Xinjiang Laboratory of Special Environmental Microbiology/Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2022-12-26 Online:2023-09-20 Published:2023-09-19
Correspondence author: ZHANG Zhidong(1977-), male, researcher, research direction for Exploration of Microbial and Probiotic Resources in Special Environments, (E-mail)zhangzheedong@qq.com
Supported by:
Xinjiang Regional Collaborative Innovation Special Project (SCO Science and Technology Partnership Program Project)(2020E01007);National Key R & D Program Project(2018YFC1603400);National Key R & D Program Project(2018YFC1603404)

摘要/Abstract

摘要：

【目的】挖掘和利用赛里木酸奶中的乳酸菌资源。【方法】采集12份阿克苏地区赛里木镇周边酸奶样品,采用平板稀释法进行菌株分离,通过16S rRNA基因序列测序和菌株形态学观察和明确菌株分类学地位,检测菌株溶血性和有害代谢物质等。【结果】在12种赛里木酸奶样品中筛选得到4种乳酸菌,分属于Eremococcus、Leuconostoc、Lentilactobacillus、 Streptococcus 4个属下的4个种,试验菌株可耐受0.5%胆盐,0.5%NaCl,且具有较好耐高温、牛奶胨化和抑制细菌等特性,并展现出良好的自聚合能力;所有菌株均无溶血现象;除H5-4外利用氨基酸均不产生物胺,并不产生硝基还原酶。【结论】从赛里木酸奶中分离获得的乳酸菌具有良好的发酵特性和食品安全性。

关键词: 赛里木酸奶; 乳酸菌; 分离筛选

Abstract:

【Objective】 To explore and utilize the LAB resources in Sayram Ketteki.【Methods】 12 yoghurt samples were collected around Sayram town in Aksu Prefecture, and the strains were isolated by the plate dilution method.The 16S rRNA gene sequences and morphological observation were carried out to clarify the taxonomic status of the strains, and edible safety characteristics such as hemolysis and harmful metabolites were detected.【Results】 A total of 36 LAB strains were isolated and identified, which belonged to 4 species of Eremococcus, Leuconostoc, Lentilactobacillus and Streptococcus; the tested strains could tolerate 0.5% bile salt, 5% NaCl, and showed good high temperature resistance, milk peptonization, different enzyme activities, antibacterial properties, and auto aggregation ability.None of strains were positive for hemolysis.Nonitroreductase and biogenic amines were produced by strains except H5-4.【Conclusion】 The LAB obtained from camel milk in this study have good fermentation characteristics and food safety, which provides a research basis for the subsequent development of lactic acid bacteria resources.

Key words: Sayram Ketteki; Lactic acid bacteria; screening

中图分类号:

S188
S879.1

何齐, 冯倩, 李雪, 易鸳鸯, 顾美英, 朱静, 孙建, 张志东. 赛里木酸奶中乳酸菌的分离鉴定及特性分析[J]. 新疆农业科学, 2023, 60(9): 2323-2330.

HE Qi, FENG Qian, LI Xue, YI Yuanyang, GU Meiying, ZHU Jin, SUN Jian, ZHANG Zhidong. Screening, identification and characteristics of Lactic acid bacteria from the sayram ketteki[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2323-2330.

图/表 7

参考文献 26

[1]	苑贝贝. 拜城赛里木酸奶营养组分及功能因子的分析[D]. 阿拉尔: 塔里木大学, 2017.
	YUAN Beibei. Analysis on nutritional ingredients and functional of sayram ketteki from Baicheng County[D]. Aral: Tarim University, 2017.
[2]	Evivie S E, Huo G C, Igene J O. Some current applications, limitations and future perspectives of Lactic acid bacteria as probiotics[J]. Food & Nutrition Research, 2017, 61(1): 1318034.
[3]	玛依诺·木图拉. 赛里木酸奶中产粘乳酸菌的分离筛选及发酵工艺的研究[D]. 南京: 南京农业大学, 2010.
	Mayinuo Mutula. Isolation and identification of eps producing lactic acid bacteria from sayram, traditional xinjiang ropy yoghurt, and its role in fermentation process[D]. Nanjing: Nanjing Agricultural University, 2010.
[4]	高冬腊. 赛里木生牛乳和酸奶微生物多样性分析及高产γ-氨基丁酸菌株的筛选[D]. 阿拉尔: 塔里木大学, 2020.
	GAO Dongla. Analysis of Microbial Diversity of Sayram Milk and Yogurt and Screening of High-yield γ-Aminobutyric Acid Strains[D]. Aral: Tarim University, 2020.
[5]	东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001: 364-370.
	DONG Xiuzhu, CAI Miaoying. Manual for identification of common bacterial systems[M]. Beijing: Science Press, 2001: 364-370.
[6]	刘晓静, 楚敏, 朱静, 等. 库车小白杏内生细菌多样性及其产酶特性[J]. 食品科学, 2020, 41(14):203-209.
	LIU Xiaojing, CHU Min, ZHU Jing, et al. Diversity and functional enzymes of endophytic bacteria in Kuqa Apricot[J]. Food Science, 2020, 41(14):203-209. DOI
[7]	Aspri M, Bozoudi D, Tsaltas D, et al. Raw donkey milk as a source of enterococcus diversity: assessment of their technological properties and safety characteristics[J]. Food Control, 2017, 73(5): 81-90. DOI URL
[8]	王梦姣. 内蒙古牧区马奶及其制品中肠球菌属乳酸菌安全性评价[D]. 呼和浩特: 内蒙古农业大学, 2014.
	WANG Mengjiao. The safety evaluation of the latic acid bacteria isolated from mare’s milk and its products in the Pastoral Areas of Inner Mongolia[D]. Huhhot: Inner Mongolia Agricultural University, 2014.
[9]	孟丹, 王丽群, 谢国梁, 等. 一株乳酸乳球菌产γ-氨基丁酸能力及其安全性评价[J]. 中国酿造, 2017, 36(4): 72-77.
	MENG Dan, WANG Liqun, XIE Guoliang, et al. γ- aminobutyric acid-producing ability and safety evaluation of Lactococcus lactis[J]. China Brewing, 2017, 36(4): 72-77.
[10]	周铄浛, 杨柳, 安彬, 等. 乳酸菌体外黏附性方法的评价及相关性研究[J]. 食品科技, 2020, 45(8):20-28.
	ZHOU Shuohan, YANG Liu, AN Bin, et al. Correlation between Methods for Evaluating the Adhesion of Lactic Acid Bacteria[J]. Food Science and Technology, 2020, 45(8):20-28.
[11]	Di G A, Francesca N, Gaglio R, et al. The influence of the wooden equipment employed for cheese manufacture on the characteristics of a traditional stretched cheese during ripening[J]. Food Microbiology, 2015, 46(Apr.):81-91. DOI URL
[12]	Wu Q, Xu Y, Chen L. Diversity of yeast species during fermentative process contributing to Chinese Maotai-flavour liquor making[J]. Letters in Applied Microbiology, 2012, 55(4).
[13]	解万翠, 尹超, 宋琳, 等. 中国传统发酵食品微生物多样性及其代谢研究进展[J]. 食品与发酵工业, 2018, 44(10):253-259.
	XIE Wancui, YIN Chao, SONG Lin, et al. Research progress of microganism and its metabolism in traditional fermentation food[J]. Food and Fermentation Industries, 2018, 44(10):253-259.
[14]	王子涵, 林青, 刘钰洁, 等. 赛里木酸奶细菌多样性及挥发性风味物质成分分析[J/OL]. 食品与发酵工业:1-9[2022-03-06].DOI:10.13995/j.cnki.11-1802/ts.030635. DOI
	WANG Zihan, LIN Qing, LIU Yujie, et al. Analysis of bacterial diversity and volatile flavor compounds in sayram yogurt[J/OL]. Food and Fermentation Industries: 1-9[2022-03-06].DOI:10.13995/j.cnki.11-1802/ts.030635. DOI
[15]	Jans C, Bugnard J, Njage P, et al. Lactic acid bacteria diversity of African raw and fermented camel milk products reveals a highly competitive, potentially health-threatening predominant microflora[J]. LWT - Food Science and Technology, 2012, 47(2):371-379. DOI URL
[16]	Saini K, Tomar S K. In vitro evaluation of probiotic potential of Lactobacillus cultures of human origin capable of selenium bioaccumulation[J]. LWT-Food Science and Technology, 2017, 84(2):497-504. DOI URL
[17]	Ji K, Jang N Y, Kim Y T. Isolation of lactic acid bacteria showing antioxidative and probiotic activities from klimchi and infant fece[J]. Journal of Microbiology and Biotechnology, 2015, 25(9): 1568-1577. DOI URL
[18]	王融, 包栩睿, 王鑫钰, 等. 鱼类肠道乳酸菌的抑菌特性及机理研究[J]. 现代化农业, 2014,(8):49-51.
	WANG Rong, BAO Yurui, WANG Xinyu, et al. Study on antibacterial characteristics and mechanism of lactic acid bacteria in fish intestine[J]. Modernizing Agriculture, 2014,(8):49-51.
[19]	蔡静静, 张亚川, 李谞, 等. 新疆伊犁地区乳制品中乳酸菌发酵和益生特性及其复合发酵方案优化[J]. 食品科学, 2020, 41(18): 172-179.
	CAI Jingjing, ZHANG Yachuan, LI Xu, et al. Fermentation and probiotic properties of lactic acid bacteria isolated from dairy products in Yili, Xinjiang and optimization of their mixtures for mixed-culture fermentation[J]. Food Science, 2020, 41(18): 172-179. DOI
[20]	夏学超, 于金换, 曲桂武. 强化发酵对辣椒中生物胺及理化指标影响的研究[J]. 食品科技, 2021, 46(10):13-18.
	XIA Xuechao, YU Jinhuan, QU Guiwu. Effect of Intensified Fermentation on Biogenic Amines and Physicochemical Indexes of Chili[J]. Food Science and Technology, 2021, 46(10):13-18.
[21]	Gow-Chin Y, Hsin K. Antioxidative Effect of Biogenic Amine on the Peroxidation of Linoleic Acid[J]. Bioscience, Biotechnology, and Biochemistry, 1993(1):1.
[22]	Fernanda G, Marisa C, Nicola C, et al. Focused Review: Agmatine in Fermented Foods[J]. Frontiers in Microbiology, 2012, 3.
[23]	田建军, 张开屏, 景智波, 等. 发酵肉制品加工中衍生的非健康因子控制研究进展[J]. 中国食品学报, 2020, 20(1):275-283.
	TIAN Jianjun, ZHANG Kaiping, JING Zhibo, et al. Recent progress on control of the unhealthy factors deriving from spontaneously fermented meat products processing[J]. Journal of Chinese Institute of Food Science and Technology, 2020, 20(1):275-283.
[24]	Jeon A R, Lee J H, Mah J H. Biogenic amine formation and bacterial contribution in Cheonggukjang, a Korean traditional fermented soybean food[J]. LWT, 2018:282-289.
[25]	陈玉峰, 吴燕燕, 李来好, 等. 腌干鱼贮藏过程生物胺的变化及其货架期研究[J]. 核农学报, 2016, 30(8):1548-1557. DOI
	CHEN Yufeng, WU Yanyan, LI Laihao, et al. Study on change of biogenic amines and its shelf life of dried-salted fish at storage[J]. Journal of Nuclear Agricultural Sciences, 2016, 30(8):1548-1557. DOI
[26]	Latorre L, Comas O, Bover S, et al. Tyramine and histamine risk assessment related to consumption of dry fermented sausages by the Spanish population[J]. Food and Chemical Toxicology, 2016:78-85.

序号 Serial number	编号 Code numble	采集地点 Sampling position	备注 Remark
1	H₁	拜城县黑英山乡亚吐尔村4组	自产
2	H₂	拜城县黑英山乡8村1组	半商品化
3	H₃	拜城县黑英山乡8村2组	自产
4	H₄	拜城县黑英山乡明布拉克村大组	自产
5	H₅	拜城县黑英山乡1村4组	自产
6	D₁	拜城县赛里木酸奶厂	商品化
7	M₁	赛里木镇2村2大队	自产
8	K₁	拜城县克孜尔乡	自产
9	S₁	拜城县赛里木酸奶厂	商品化
10	S₄	赛里木镇托喀其买里村	半商品化
11	S₅	赛里木镇6村1组	自产
12	S₆	赛里木镇2村2大队	自产

序号 Serial number	编号 Code numble	采集地点 Sampling position	备注 Remark
1	H₁	拜城县黑英山乡亚吐尔村4组	自产
2	H₂	拜城县黑英山乡8村1组	半商品化
3	H₃	拜城县黑英山乡8村2组	自产
4	H₄	拜城县黑英山乡明布拉克村大组	自产
5	H₅	拜城县黑英山乡1村4组	自产
6	D₁	拜城县赛里木酸奶厂	商品化
7	M₁	赛里木镇2村2大队	自产
8	K₁	拜城县克孜尔乡	自产
9	S₁	拜城县赛里木酸奶厂	商品化
10	S₄	赛里木镇托喀其买里村	半商品化
11	S₅	赛里木镇6村1组	自产
12	S₆	赛里木镇2村2大队	自产

菌株 Strains	温度 Temperature (℃)		NaCl(%)			pH		胆盐 Bile salt (%)	牛奶胨化 Milk peptonization
菌株 Strains	20	45	3	5	7	3	4	0.5	胨化 Peptization	产香 Fragrance
S5-1	+	+	+	+	+	-	w	++	+	+
D1-3	+	+	+	+	-	-	w	++	+	+
H4-2	+	+	+	+	-	-	w	++	+	++
H5-4	+	+	+	+	-	-	w	++	+	+

菌株 Strains	温度 Temperature (℃)		NaCl(%)			pH		胆盐 Bile salt (%)	牛奶胨化 Milk peptonization
菌株 Strains	20	45	3	5	7	3	4	0.5	胨化 Peptization	产香 Fragrance
S5-1	+	+	+	+	+	-	w	++	+	+
D1-3	+	+	+	+	-	-	w	++	+	+
H4-2	+	+	+	+	-	-	w	++	+	++
H5-4	+	+	+	+	-	-	w	++	+	+

菌株 Strains	功能酶 Functional enzymes
菌株 Strains	蛋白酶 Protease	淀粉酶 Amylase	果胶酶 Pectase	酯化酶 Esterase	纤维素酶 Cellulase
S5-1	+	-	-	-	-
D1-3	+	-	-	-	-
H4-2	+	-	-	-	-
H5-4	+	-	-	-	-

赛里木酸奶中乳酸菌的分离鉴定及特性分析

Screening, identification and characteristics of Lactic acid bacteria from the sayram ketteki

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参考文献 26

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Metrics

菌株 Strains	病原指示菌 Pathogen indicator
菌株 Strains	大肠埃希菌 E.coli	枯草芽孢杆菌 B.subtilis	金黄色葡萄球菌 S.aureus	青霉菌 Penicillium	黑曲霉 Asp.niger	白色念珠菌 C.albicans
S5-1	++	-	+	-	-	-
D1-3	+++	+	++	-	-	-
H4-2	+++	++	+++	-	-	+
H5-4	+	+	+	-	-	-

菌株 Strains	生物胺 Biogenic amines					吲哚 Indoles	溶血性 hemolytic	硝基还原酶 Nitroreductase
菌株 Strains	精胺 Spermine	酪胺 Tyramine	色胺 Tryptamine	尸胺 Cadaverine	组胺 Histamine	吲哚 Indoles	溶血性 hemolytic	硝基还原酶 Nitroreductase
S5-1	-	-	-	-	-	-	-	-
D1-3	-	-	-	-	-	-	-	-
H4-2	-	-	-	-	-	-	-	-
H5-4	-	-	+	-	-	-	-	+
^*E.coli	(CK: -)					+	+	+

[1]	贺婷婷, 王旭哲, 宋磊, 马春晖. 不同添加剂对油莎豆青贮品质及有氧稳定性的影响[J]. 新疆农业科学, 2022, 59(7): 1767-1775.
[2]	伊力夏提·艾热提, 李伟, 徐杨林, 严宏孟, 戴志伟, 周建中. 传统发酵酸凝硬质奶酪中乳酸菌的分离鉴定及其体外益生特性[J]. 新疆农业科学, 2022, 59(6): 1491-1501.
[3]	刘晓蓉;向廷建;逄焕明;古丽娜孜;李瑾瑜;王子荣. 乌昌地区传统酸牛奶中乳酸菌的分离鉴定[J]. , 2013, 50(8): 1434-1441.
[4]	唐血梅;李海英;赵芳;姚新奎;董婷;车驰;祝春梅;刘婷婷;曾亚琦;韩煜茹;曹丽萍;王建文;刘志安;孟军;李智军. 新疆酸马奶中高产胞外多糖乳酸菌筛选鉴定及培养条件优化研究[J]. , 2012, 49(8): 1540-1545.
[5]	任志娟;李轶杰;马正海. 乳酸乳球菌质粒提取方法的改进与优化[J]. , 2011, 48(4): 734-738.
[6]	陈阳;姚新奎;潘道东;曾小群. 一株酸马奶乳酸菌分子生物学鉴定及降解胆固醇试验研究[J]. , 2009, 46(5): 1121-1125.
[7]	张晓琳;王炜;张广敏;常玮;孔健. 类开菲尔粒的发酵特性及强产酸乳酸菌的分离鉴定[J]. , 2009, 46(4): 856-860.
[8]	傅力;葛钰瑛;李焕荣;朱正兰;桑昆. 发酵羊肉乳酸菌发酵剂的筛选及发酵性能研究[J]. , 2009, 46(3): 630-634.
[9]	熊素玉;姚新奎;谭小海;刘珩;张积荣;陈潇;许鹏;熊伟. 酸马奶中乳酸菌的分离、纯化与鉴定[J]. , 2007, 44(5): 696-701.
[10]	熊素玉;姚新奎;谭小海;刘珩;张积荣;曾波;许鹏;熊伟;吴津蓉. 不同温度及pH条件对乳酸菌生长影响的研究[J]. , 2006, 43(6): 533-538.
[11]	黄玲. 对番茄酱中乳酸菌培养基的筛选研究[J]. , 2004, 41(z1): 90-91.