HPLC法测定番茄红素胶束中番茄红素Z/E异构体含量

doi:10.6048/j.issn.1001-4330.2022.09.015

摘要/Abstract

摘要：

【目的】 测定番茄红素胶束中番茄红素Z/E异构体含量。【方法】 采用HPLC法，色谱柱为YMC Carotenoid S-5（4.6 mm×250 mm）；流动相 A：乙腈B：甲基叔丁基醚；线性梯度洗脱；流速：1 mL/min；检测波长：472 nm；柱温：30 ℃，在45 min内完成异构体的分离检测，通过紫外-可见吸收光谱图上的特征峰及吸收强度,分析和鉴定番茄红素胶束中存在的异构体。【结果】 番茄红素在0.22~6.57 μg/mL 线性关系良好（r=0.999）；加样回收率为100.06%（RSD=1.99%），该方法专属性强，线性关系良好，快速、准确、简便，适用于番茄红素胶束中异构体的含量测定。【结论】 番茄红素胶束中存在全反式：All-E，以及三个顺式异构体：13 Z，9 Z，5 Z，其平均相对百分含量分别为76.06%、10.83%、4.55%、8.56%。

关键词: 番茄红素; 胶束; 异构体; 含量测定

Abstract:

【Objective】 To determine the content of Z/E isomers in lycopene micelles. 【Method】 HPLC method， The column was YMC Carotenoid S-5（4.6 mm×250 mm）； Mobile phase A：acetonitrile B：methyl tert-butyl ether； Linear gradient elution； Flow rate：1 mL/min； Detection wavelength：472 nm； Column temperature：30℃， Complete the separation and detection of isomers within 45 minutes， the isomers in the lycopene micelles are analyzed and identified through the characteristic peaks and absorption intensity on the ultraviolet-visible absorption spectrum.【Result】 The linear ranges of Lycopene was 0.22~6.57 μg/mL （r=0.999）， Whose average recoveries were 100.06%（RSD=1.99%）. It shows that the method has strong specificity， good linear relationship， fast， accurate and simple， and is suitable for the determination of isomers in lycopene micelles.【Conclusion】 Determined that there are all-trans forms in the lycopene micelles：All-E， and three cis isomers：13Z， 9Z， 5Z.with an average relative percentage of 76.06%，10.83%，4.55%，8.56%， respectively.

Key words: lycopene; micelles; isomers; content determination

中图分类号:

S641.2

马雪红, 刘婷, 李传天, 朱金芳. HPLC法测定番茄红素胶束中番茄红素Z/E异构体含量[J]. 新疆农业科学, 2022, 59(9): 2209-2216.

MA Xuehong, LIU Ting, LI Chuantian, ZHU Jinfang. Determination of Z/E Isomer Content in Lycopene Micelle by HPLC[J]. Xinjiang Agricultural Sciences, 2022, 59(9): 2209-2216.

图/表 10

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放置时间（h） Put time （h）	含量（μg/mL） Content （μg/mL）	平均含量（μg/mL） Average content （μg/mL）	RSD（%）
0	376	371	0.69
1	369
2	369
4	372
6	370
8	372
12	370
24	368

放置时间（h） Put time （h）	含量（μg/mL） Content （μg/mL）	平均含量（μg/mL） Average content （μg/mL）	RSD（%）
0	376	371	0.69
1	369
2	369
4	372
6	370
8	372
12	370
24	368

分组 Grouping	样品中量 Sample volume （μg）	加入量 Amount added （μg）	测得量 Measured amount （μg）	回收率 Recovery rate （%）	平均值 Average value （%）	RSD （%）
低 Low	185	148	328	97.30	100.06	1.99
	185	148	332	99.46
	185	148	337	102.16
中 Middle	185	185	371	100.54
	185	185	370	100.00
	185	185	372	101.08
高 High	185	222	401	96.76
	185	222	412	102.70
	185	222	408	100.54

分组 Grouping	样品中量 Sample volume （μg）	加入量 Amount added （μg）	测得量 Measured amount （μg）	回收率 Recovery rate （%）	平均值 Average value （%）	RSD （%）
低 Low	185	148	328	97.30	100.06	1.99
	185	148	332	99.46
	185	148	337	102.16
中 Middle	185	185	371	100.54
	185	185	370	100.00
	185	185	372	101.08
高 High	185	222	401	96.76
	185	222	412	102.70
	185	222	408	100.54

批号 Batch number	含量（μg/mL） Content	平均含量（μg/mL） Average content	批内 RSD（%） RSD within batch	批间 RSD（%） RSD between batches
20200801	373	371	0.41	0.54
	372
	370
20200802	372	369	0.63
	368
	368
20200803	373	372	0.56
	370
	369