新疆农业科学 ›› 2021, Vol. 58 ›› Issue (12): 2274-2281.DOI: 10.6048/j.issn.1001-4330.2021.12.015

• 植物保护·园艺特产·土壤肥料·节水灌溉·农业生态环境·农业装备工程与机械化 • 上一篇    下一篇

高温胁迫对葡萄叶绿素荧光和光合特性参数的影响

吴久赟1(), 徐桂香1, 李海峰1, 曾晓燕2, 姜建福3, 刘勇翔3, 魏亦农2, 任红松1()   

  1. 1.新疆农业科学院吐鲁番农业科学研究所,新疆吐鲁番 838000
    2.石河子大学农学院,新疆石河子 832000
    3.中国农业科学院郑州果树研究所,河南郑州 450009
  • 收稿日期:2020-10-18 出版日期:2021-12-20 发布日期:2021-12-31
  • 通讯作者: 任红松
  • 作者简介:吴久赟(1988-),男,重庆人,副研究员,硕士,研究方向为葡萄种质的收集、保存、鉴定评价,(E-mail) kobewjy@163.com
  • 基金资助:
    新疆维吾尔自治区天山青年计划(2018Q093);新疆维吾尔自治区天山青年计划(2019Q091);新疆维吾尔自治区自然科学基金项目(2019D01B33);新疆维吾尔自治区天山创新团队(2019D01B33)

Effects of Heat Stress on Chlorophyll Fluorescence and Photosynthetic Characteristic Parameters in Grape(Vitisvinifera L.'Manicure finger')

WU Jiuyun1(), XU Guixiang1, LI Haifeng1, ZENG Xiaoyan2, JIANG Jianfu3, LIU Yongxiang3, WEI Yinong2, REN Hongsong1()   

  1. 1. Turpan Research institute of Agricultural Sciences, Xinjiang Academy of Agricultural Sciences, Turpan Xinjiang 838000, China
    2. College of Agronomy, Shihezi University, Shihezi Xinjiang 832000, China
    3. Zhengzhou Fruit Research Institute, CAAS, Zhengzhou 450009, China
  • Received:2020-10-18 Online:2021-12-20 Published:2021-12-31
  • Contact: REN Hongsong
  • Supported by:
    Tianshan Youth R & D Project of Xinjiang Uygur Autonomous Region "Effects of High Temperature Stress on Physiological Characteristics of Grape"(2018Q093);Tianshan Youth R & D Project of Xinjiang Uygur Autonomous Region "Effects of High Temperature Stress on Physiological Characteristics of Grape"(2019Q091);Natural Science Foundation of Xinjiang Uygur Autonomous Region "Evaluation of High Temperature Response(2019D01B33);Heat Resistance of Grape Variety"(2019D01B33)

摘要: 目的 研究高温胁迫对葡萄的叶绿素荧光和光合特性的影响,分析葡萄在高温胁迫下的响应机制,为葡萄逆境栽培和耐热机理提供参考。方法 以1年生盆栽葡萄美人指为材料,在35、40和45℃植物培养箱中连续处理16 h,以25℃培养的植株作为对照,测定葡萄叶绿素荧光动力学曲线和参数以及光合气体交换参数,分析高温胁迫对葡萄叶绿素荧光和光合特性参数的影响。结果 (1)高温胁迫16 h后导致美人指葡萄Pn降低的原因是非气孔因子导致的,解除胁迫并恢复8 h后,气体交换参数可恢复正常水平;(2)40和45℃高温会导致美人指的OJIP曲线上的荧光值降低,尤其是在J-I相和I-P相上显著低于CK;(3)35℃时美人指的Fv/Fm值与CK无显著差异,但超过40℃时Fv/Fm值显著降低;(4)高温条件下F0VjABS/RCDI0/CS0升高,FmFv/F0Fv/FmPIABSTR0/CSmET0/CSmψoφE0降低,影响了PSII中心捕获电子和电子传递,导致PSII光化学转化效率降低和热耗散比率增加。结论 高温胁迫下美人指葡萄通过降低PSII的光能吸收、量子产量和电子传递,促进热耗散,降低PSII的光化学效率。

关键词: 葡萄, 高温胁迫, 叶绿素荧光, 光合特性

Abstract:

【Objective】 The objective of this research is to study the effects of chlorophyll fluorescence and photosynthetic characteristic parameters of grape leaves under heat stress, and to explore the response mechanism of grapevines to heat stress in the hope of providing a theoretical basis forresearchingheat tolerance mechanism of grape and taking appropriate cultivation managements to reduce the impact of high temperature.【Methods】 The annual grapevines Manicure finger(VitisviniferaL.cv.)were selected and cultured in the artificial climate chamber, which were treated at 35℃ , 40℃and 45℃ for 16 h, with the plants at 25℃ as the control to determine OJIP curve, chlorophyll fluorescence and gas exchange parameters in grape leaves and analyze the effects of chlorophyll fluorescence and photosynthetic characteristics parameters of grape leaves under heat stress.【Results】 (1) The Pn decrease of Manicure finger grape were not caused by stomatal factor after 16 h heat stress, and the gas exchange parameters would be restored to normal after heat stress relieved for 8h; (2) When temperature exceeded 40℃, the fluorescence value of Manicure finger grape decreased on the OJIP curve, especially in J-I phase and I-P phase, which was significantly lower than that of CK.(3) The Fv/Fm value of Manicure finger grape were not significantly different at 35℃, but the Fv/Fm value were decreased significantly when it exceeded 40℃; (4) In the high temperatures condition, the F0, Vj, ABS/RC, DI0/CS0 were increased, the Fm,Fv/F0,Fv/Fm,PIABS,TR0/CSm,ET0/CSm,ψo,φE0 were decreased, which affected the electron capture and transfer in PSII center, leading to the decrease of photochemical conversion efficiency and the increase of heat dissipation ratio in PSII.【Conclusion】 Under high temperature stress, the grape ‘Manicure finger’ can reduce the photochemical efficiency of PSII by reducing the absorption of light energy, quantum yield and electron transfer, thus reducing the photochemical efficiency of PSII.

Key words: grape, heat stress, chlorophyll fluorescence, physiological characteristics

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