新疆农业科学 ›› 2021, Vol. 58 ›› Issue (12): 2274-2281.DOI: 10.6048/j.issn.1001-4330.2021.12.015
• 植物保护·园艺特产·土壤肥料·节水灌溉·农业生态环境·农业装备工程与机械化 • 上一篇 下一篇
吴久赟1(), 徐桂香1, 李海峰1, 曾晓燕2, 姜建福3, 刘勇翔3, 魏亦农2, 任红松1(
)
收稿日期:
2020-10-18
出版日期:
2021-12-20
发布日期:
2021-12-31
通信作者:
任红松(1977-),男,新疆乌鲁木齐人,研究员,博士,研究方向为葡萄种质的收集、保存与利用,(E-mail)1134037950@qq.com作者简介:
吴久赟(1988-),男,重庆人,副研究员,硕士,研究方向为葡萄种质的收集、保存、鉴定评价,(E-mail) kobewjy@163.com
基金资助:
WU Jiuyun1(), XU Guixiang1, LI Haifeng1, ZENG Xiaoyan2, JIANG Jianfu3, LIU Yongxiang3, WEI Yinong2, REN Hongsong1(
)
Received:
2020-10-18
Published:
2021-12-20
Online:
2021-12-31
Correspondence author:
REN Hongsong(1977-),PhD, Researcher, Engaged in the collection, preservation and utilization of grape Germplasm,(E-mail)1134037950@qq.comSupported by:
摘要: 目的 研究高温胁迫对葡萄的叶绿素荧光和光合特性的影响,分析葡萄在高温胁迫下的响应机制,为葡萄逆境栽培和耐热机理提供参考。方法 以1年生盆栽葡萄美人指为材料,在35、40和45℃植物培养箱中连续处理16 h,以25℃培养的植株作为对照,测定葡萄叶绿素荧光动力学曲线和参数以及光合气体交换参数,分析高温胁迫对葡萄叶绿素荧光和光合特性参数的影响。结果 (1)高温胁迫16 h后导致美人指葡萄Pn降低的原因是非气孔因子导致的,解除胁迫并恢复8 h后,气体交换参数可恢复正常水平;(2)40和45℃高温会导致美人指的OJIP曲线上的荧光值降低,尤其是在J-I相和I-P相上显著低于CK;(3)35℃时美人指的Fv/Fm值与CK无显著差异,但超过40℃时Fv/Fm值显著降低;(4)高温条件下F0、Vj、ABS/RC、DI0/CS0升高,Fm、Fv/F0、Fv/Fm、PIABS、TR0/CSm、ET0/CSm、ψo、φE0降低,影响了PSII中心捕获电子和电子传递,导致PSII光化学转化效率降低和热耗散比率增加。结论 高温胁迫下美人指葡萄通过降低PSII的光能吸收、量子产量和电子传递,促进热耗散,降低PSII的光化学效率。
中图分类号:
吴久赟, 徐桂香, 李海峰, 曾晓燕, 姜建福, 刘勇翔, 魏亦农, 任红松. 高温胁迫对葡萄叶绿素荧光和光合特性参数的影响[J]. 新疆农业科学, 2021, 58(12): 2274-2281.
WU Jiuyun, XU Guixiang, LI Haifeng, ZENG Xiaoyan, JIANG Jianfu, LIU Yongxiang, WEI Yinong, REN Hongsong. Effects of Heat Stress on Chlorophyll Fluorescence and Photosynthetic Characteristic Parameters in Grape(Vitisvinifera L.'Manicure finger')[J]. Xinjiang Agricultural Sciences, 2021, 58(12): 2274-2281.
处理 Treatment | F0 | Fm | Fv/F0 | Fv/Fm |
---|---|---|---|---|
35℃ | 405.40±37.29abAB | 1 494.80±76.23aA | 2.71±0.31bA | 0.73±0.02aA |
40℃ | 398.86±98.16abcAB | 1 012.00±409.70bB | 1.48±0.78cB | 0.56±0.14bB |
45℃ | 447.31±72.20aA | 878.77±158.64bB | 0.99±0.43dB | 0.48±0.10cB |
CK | 348.57±37.98bdB | 1546.21±130.05aA | 3.47±0.44aA | 0.77±0.02aA |
表1 不同温度下葡萄叶片F0、Fm、Fv/F0、Fv/Fm变化
Table 1 Effects of high temperature on F0, Fm, Fv/F0, Fv/Fm of grape leaves
处理 Treatment | F0 | Fm | Fv/F0 | Fv/Fm |
---|---|---|---|---|
35℃ | 405.40±37.29abAB | 1 494.80±76.23aA | 2.71±0.31bA | 0.73±0.02aA |
40℃ | 398.86±98.16abcAB | 1 012.00±409.70bB | 1.48±0.78cB | 0.56±0.14bB |
45℃ | 447.31±72.20aA | 878.77±158.64bB | 0.99±0.43dB | 0.48±0.10cB |
CK | 348.57±37.98bdB | 1546.21±130.05aA | 3.47±0.44aA | 0.77±0.02aA |
处理Treatment(℃) | PIabs | Vj | ABS/RC | DI0/CSm |
---|---|---|---|---|
35 | 1.56±0.91bAB | 0.52±0.13bB | 1.93±0.27bcB | 405.40±37.29abAB |
40 | 0.59±0.83bB | 0.58±0.16bAB | 2.60±0.55abAB | 398.86±98.16abcAB |
45 | 0.18±0.20bB | 0.73±0.12aA | 3.10±0.60aA | 447.31±72.20aA |
CK | 5.26±4.49aA | 0.37±0.11cC | 1.71±1.00cB | 348.57±37.98bdB |
表2 不同温度下葡萄叶片PIABS、Vj、ABS/RC、DI0/CS0变化
Table 2 Effects of high temperature on PIABS, Vj, ABS/RC, DI0/C S0 of grape
处理Treatment(℃) | PIabs | Vj | ABS/RC | DI0/CSm |
---|---|---|---|---|
35 | 1.56±0.91bAB | 0.52±0.13bB | 1.93±0.27bcB | 405.40±37.29abAB |
40 | 0.59±0.83bB | 0.58±0.16bAB | 2.60±0.55abAB | 398.86±98.16abcAB |
45 | 0.18±0.20bB | 0.73±0.12aA | 3.10±0.60aA | 447.31±72.20aA |
CK | 5.26±4.49aA | 0.37±0.11cC | 1.71±1.00cB | 348.57±37.98bdB |
处理Treatment(℃) | TR0/CSm | ET0/CSm | ψo | φE0 |
---|---|---|---|---|
35 | 1 089.40±64.78aA | 527.80±147.18bA | 0.48±0.13abAB | 0.35±0.10bA |
40 | 613.14±334.89bB | 242.29±179.36cB | 0.42±0.16bB | 0.23±0.11cB |
45 | 431.46±148.24cB | 121.23±85.59dB | 0.27±0.12cC | 0.13±0.08dB |
CK | 1 197.64±118.91aA | 750.57±160.84aA | 0.63±0.11aA | 0.48±0.09aA |
表3 不同温度下葡萄叶片TR0/CSm、ET0/CSm、ψo、φ E0变化
Table 3 Effects of high temperature on TR0/CSm, ET0/CSm, ψo, φ S0 of grape leaves
处理Treatment(℃) | TR0/CSm | ET0/CSm | ψo | φE0 |
---|---|---|---|---|
35 | 1 089.40±64.78aA | 527.80±147.18bA | 0.48±0.13abAB | 0.35±0.10bA |
40 | 613.14±334.89bB | 242.29±179.36cB | 0.42±0.16bB | 0.23±0.11cB |
45 | 431.46±148.24cB | 121.23±85.59dB | 0.27±0.12cC | 0.13±0.08dB |
CK | 1 197.64±118.91aA | 750.57±160.84aA | 0.63±0.11aA | 0.48±0.09aA |
Correlations | Pn | Gs | Ci | Tr |
---|---|---|---|---|
Pn | 1.000 | |||
Gs | 0.725** | 1.000 | ||
Ci | -0.673** | -0.128 | 1.000 | |
Tr | 0.677** | 0.969** | -0.090 | 1.000 |
表4 相关性
Table 4 Correlations analysis on Pn,Gs, Ci, Tr of grape leaves
Correlations | Pn | Gs | Ci | Tr |
---|---|---|---|---|
Pn | 1.000 | |||
Gs | 0.725** | 1.000 | ||
Ci | -0.673** | -0.128 | 1.000 | |
Tr | 0.677** | 0.969** | -0.090 | 1.000 |
处理 Treat ment | Pn | Gs | Ci | Tr |
---|---|---|---|---|
T | 5.31±2.10aA | 0.05±0.02aA | 277.99±40.53aA | 1.13±0.52aA |
CK | 5.48±1.42aA | 0.08±0.04aA | 323.83±29.75aA | 1.85±0.89aA |
表5 恢复8h后葡萄叶片的光合气体交换参数
Table 5 Photosynthetic gas exchange parameters in grape leaves after 8 h recovery
处理 Treat ment | Pn | Gs | Ci | Tr |
---|---|---|---|---|
T | 5.31±2.10aA | 0.05±0.02aA | 277.99±40.53aA | 1.13±0.52aA |
CK | 5.48±1.42aA | 0.08±0.04aA | 323.83±29.75aA | 1.85±0.89aA |
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