棉铃虫鞣化激素β亚基参与脂肪组织免疫的相关功能基因表达模式

doi:10.6048/j.issn.1001-4330.2020.04.002

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (4): 600-607.DOI: 10.6048/j.issn.1001-4330.2020.04.002

棉铃虫鞣化激素β亚基参与脂肪组织免疫的相关功能基因表达模式

嵇玉洁, 马星宇, 宋永辉, 杜孟芳, 魏纪珍, 尹新明, 刘晓光, 安世恒

河南农业大学植物保护学院/省部共建小麦玉米作物学国家重点实验室, 郑州 450002

收稿日期:2019-11-04 发布日期:2020-03-26
通信作者: 刘晓光(1981-),男,河南舞阳人,副教授,博士,硕士生导师,研究方向为昆虫生理生化,(E-mail)xgliu2000@aliyun.com
作者简介:嵇玉洁(1993-),女,硕士研究生, 研究方向为昆虫生理生化,(E-mail)jiyujie2017@163.com
基金资助:
国家重点研发计划“棉花化肥农药减施技术集成研究与示范” (2017YDF0201900)和“黄淮海夏玉米化肥农药减施技术集成研究与示范”(2018YFD0200600));河南农业大学校创新项目(KJCX2017A11);国家自然科学基金(31601904);中科院昆虫发育与进化重点实验室开放课题(2009DP17321425-IDEB-KF-0021)

Molecular Characterization and Gene Expression Profile of Bursicon-β Subunit Gene Related to Innate Immune Responses in Larval Fat Body of Helicoverpa armigera

JI Yujie, MA Xingyu, SONG Yonghui, DU Mengfang, WEI Jizhen, YIN Xinming, LIU Xiaoguang, AN Shiheng

State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China

Received:2019-11-04 Published:2020-03-26
Correspondence author: LIU Xiaoguang(1981-)male,native place, Henan, Wuyang, pHD,masters supervisor,research field: Insect physiology and Biochemistry,(E-mail)xgliu2000@aliyun.com
Supported by:
Support by National Key R&D Program of China“Fertilizer and pesticide reduction and efficiency technology integration and demonstration of cotton”(2017YDF0201900) and“Fertilizer and pesticide reduction and efficiency technology integration and demonstration of summer maize in Huang huai hai region”(2018YFD0200600); Innovation fund of Henan Agricultural University“Molecular characterization and functional analysis of tachykinin receptor gene in Helicoverpa armigera (Lepidoptera: Noctuidae)”(KJCX2017A11); National Natural Science Foundation of China“Studies on the molecular mechanism of modulation of lipid metabolism by tachykinin in Helicoverpa armigera midgut”(31601904);Open Project Program of key laboratory for insect development and evolution of Chinese Academy of Sciences(2009DP17321425-IDEB-KF-0021)

摘要/Abstract

摘要： 【目的】研究棉铃虫鞣化激素β亚基参与脂肪组织免疫的相关功能基因表达模式。【方法】利用多肽激素体外处理与活体RNAi验证,将保存的鞣化激素β亚基同聚体重组蛋白,体外处理棉铃虫幼虫脂肪体组织,分别处理0、0.5、1和3 h,及体内注射鞣化激素β亚基dsRNA,72 h后取样,通过qRT-PCR检测棉铃虫幼虫脂肪体相关免疫基因表达模式。【结果】鞣化激素β亚基同源二聚体蛋白体外处理棉铃虫幼虫脂肪体后,免疫相关基因cecropin1、cecropin2、cecropin3、attacin、cecropin D、gloverin、defensin、moricin和lebocin在 0.5 h转录水平迅速上升,随后在1 h恢复至初始水平,在3 h与初始转录水平亦无显著变化,具有相同的变化趋势;棉铃虫体内注射鞣化激素Burs- β亚基dsRNA,72 h后,moricin转录水平无显著差异, cecropin1、cecropin2、cecropin3、attacin、cecropin D、gloverin、defensin、moricin和lebocin等8个免疫相关基因转录水平的表达均下调。【结论】鞣化激素β亚基同聚体蛋白质能够参与棉铃虫的免疫相关基因转录。

关键词: 棉铃虫; 鞣化激素β亚基; 免疫反应; RNAi

Abstract: 【Objective】 To investigate whether the Bursicon- β subunit homopolymer protein is involved in the immune function in larval Helicoverpa armigera.【Methods】 Using the strategy of in vitro treatment of peptide hormones and RNAi of live larvae, the Bursicon β-subunit homomeric recombinant protein stored in this experiment was used to treat the Helicoverpa armigera larva fat body tissue in vitro, respectively, 0 h, 0.5 h, 1 h, 3 h, and The Bursicon β-subunit dsRNA was injected in vivo. The expression pattern of associated immune genes in Helicoverpa armigera was studied by qRT-PCR.【Results】 When the Bursicon β-subunit homodimer protein treated Helicoverpa armigera larvae fat body tissue in vitro, the immune-related genes cecropin1, cecropin2, cecropin3, attacin, cecropin D, gloverin, defensin, moricin, and lebocin increased rapidly at 0.5 h, and subsequently it returned to the initial level at 1 h.Then there was no significant change at 3 h with the initial transcription level, with the same trend. when the Burs-β subunit dsRNA was injected into the Helicoverpa armigera,there was no significant difference in the transcription level of moricin and the expression levels of other lipid-immunity-related genes were down-regulated.【Conclusion】 The Bursicon- β subunit homopolymer protein can participate in the transcription of immune-related genes in larval of H. armigera.

Key words: Helicoverpa armigera; Bursicon beta subunit; immune response; RNAi

中图分类号:

S435.622⁺.9

引用本文

嵇玉洁, 马星宇, 宋永辉, 杜孟芳, 魏纪珍, 尹新明, 刘晓光, 安世恒. 棉铃虫鞣化激素β亚基参与脂肪组织免疫的相关功能基因表达模式[J]. 新疆农业科学, 2020, 57(4): 600-607.

JI Yujie, MA Xingyu, SONG Yonghui, DU Mengfang, WEI Jizhen, YIN Xinming, LIU Xiaoguang, AN Shiheng. Molecular Characterization and Gene Expression Profile of Bursicon-β Subunit Gene Related to Innate Immune Responses in Larval Fat Body of Helicoverpa armigera[J]. Xinjiang Agricultural Sciences, 2020, 57(4): 600-607.

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棉铃虫鞣化激素β亚基参与脂肪组织免疫的相关功能基因表达模式

Molecular Characterization and Gene Expression Profile of Bursicon-β Subunit Gene Related to Innate Immune Responses in Larval Fat Body of Helicoverpa armigera

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[5]	于健, 查萌, 郑梦君, 刘小侠. 白僵菌和绿僵菌对棉铃虫的室内防控效果评价[J]. 新疆农业科学, 2020, 57(4): 608-615.
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