四氯虫酰胺对棉铃虫生物活性的温度效应及其解毒酶相关机制

doi:10.6048/j.issn.1001-4330.2021.08.010

新疆农业科学 ›› 2021, Vol. 58 ›› Issue (8): 1447-1453.DOI: 10.6048/j.issn.1001-4330.2021.08.010

• 棉花化肥农药减施专栏 • 上一篇下一篇

四氯虫酰胺对棉铃虫生物活性的温度效应及其解毒酶相关机制

窦亚楠¹, 孙小诺², 安静杰¹, 高占林¹, 郭江龙¹, 党志红¹, 李耀发¹

1.河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业农村部华北北部作物有害生物综合治理重点实验室,河北保定 071000;
2.保定市气象局,河北保定 071000

收稿日期:2021-01-28 出版日期:2021-08-20 发布日期:2021-08-09
通信作者: 李耀发(1977-),男,河北高碑店人,研究员,博士,研究方向为农业昆虫与害虫防治,(E-mail)liyaofa@126.com
作者简介:窦亚楠(1993-),女,河北唐县人,硕士研究生,研究方向为农药学,(E-mail)1304894663@qq.com
基金资助:
国家重点研发计划(2017YFD0201906);河北省棉花产业技术体系(HBCT2018040204)

Temperature Effect of Tetrachlorantraniliprole on the Biological Activity to Helicoverpa armigera Hübner and the Related Mechanism on Detoxification Enzymes

DOU Yanan¹, SUN Xiaonuo², AN Jingjie¹, GAO Zhanlin¹, GUO Jianglong¹, DANG Zhihong¹, LI Yaofa¹

1. Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences/ IPM Center of Hebei Province/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, MOARA, P.R.China, Baoding Hebei 071000, China;
2. Baoding Meteorological Bureau of Hebei Province, Baoding Hebei 071000, China

Received:2021-01-28 Online:2021-08-20 Published:2021-08-09
Correspondence author: LI Yaofa (1977-), male, native place: Gaobeidian, Hebei. Researcher, Ph.D. Research directions: agricultural insect and pest control, (E-mail) liyaofa@126.com
Supported by:
National Key R & D Program Project (2017YFD0201906)and the Project of Cotton Industry Technical System of Hebei Province(HBCT2018040204)

摘要/Abstract

摘要： 【目的】研究四氯虫酰胺对棉铃虫生物活性的温度效应及其影响程度,分析棉铃虫体内4种解毒酶系对该温度效应的影响机制。【方法】采用浸叶法,分别测定15、20、25、30和35℃条件下,四氯虫酰胺对棉铃虫的室内生物活性,测定四氯虫酰胺亚致死剂量下,棉铃虫体内羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GST)、多功能氧化酶(MFO)和尿苷二磷酸糖基转移酶(UGT)比活力的变化。【结果】四氯虫酰胺对棉铃虫的生物活性具有明显的正温度效应,与15℃时相比,其温度系数从+5.52增长为+1 257.35。四氯虫酰胺对棉铃虫的正温度效应可能与GST和MFO有关,其中GST在较低温下的活性变化尤为显著,而CarE和UGT则未发现与四氯虫酰胺生物活性的正温度效应明显相关。【结论】四氯虫酰胺对棉铃虫表现为明显的正温度系数药剂,高温时使用生物活性最佳,低温时抑制GST活性,可能会提高四氯虫酰胺对棉铃虫的生物活性。

关键词: 棉铃虫; 温度效应; 四氯虫酰胺; 解毒酶; 生物活性

Abstract: 【Objective】 To clarify the temperature effect and its degree on the biological activity of tetrachlorantraniliprole to Helicoverpa armigera, and to explore the influence mechanism of four detoxification enzymes of H. armigera to this temperature effect.【Methods】 The authors respectively determined the indoor biological activities of tetrachlorantraniliprole to H.armigera at 15, 20, 25, 30 and 35℃ with the leaf dipping method, and then determined the specific activities of carboxyl esterase (CarE), glutathione S-transferase (GST), multifunctional oxidase (MFO) and UDP-glycosyltransferase (UGT) of H.armigera treated by the sublethal doses of tetrachlorantraniliprole.【Results】 Tetrachlorantraniliprole had a significant positive temperature effect on the biological activity to H.armigera. Compared with 15℃, the temperature coefficient increased from +5.52 to +1,257.35. From the study about the mechanism of the four detoxification enzymes of H.armigera to the temperature effect, it was found that the positive temperature effect might be related to GST and MFO. Among them, the activity of GST at lower temperatures changed more significantly, while CarE and UGT were not clearly related to the positive temperature effect on the biological activity of tetrachlorantraniliprole. 【Conclusion】 Tetrachlorantraniliprole was an insecticide with an obviously positive temperature coefficient to H. armigera and the biological activity would be best when used at high temperature. The biological activity of tetrachlorantraniliprole against H. armiger may increase when the activity of GST is inhibited at low temperature.

Key words: Helicoverpa armigera; temperature effect; tetrachlorantraniliprole; detoxification enzyme; biological activity

中图分类号:

S482.3⁺9

窦亚楠, 孙小诺, 安静杰, 高占林, 郭江龙, 党志红, 李耀发. 四氯虫酰胺对棉铃虫生物活性的温度效应及其解毒酶相关机制[J]. 新疆农业科学, 2021, 58(8): 1447-1453.

DOU Yanan, SUN Xiaonuo, AN Jingjie, GAO Zhanlin, GUO Jianglong, DANG Zhihong, LI Yaofa. Temperature Effect of Tetrachlorantraniliprole on the Biological Activity to Helicoverpa armigera Hübner and the Related Mechanism on Detoxification Enzymes[J]. Xinjiang Agricultural Sciences, 2021, 58(8): 1447-1453.

参考文献

[1] Wu K M , Lu Y H , Feng H Q , et al. Suppression of cotton bollworm in multiple crops in China in areas with Bt toxin-containing cotton [J]. China Basic Science, 2008, 321(5896):1676-1678.
[2] 张丹丹, 杨现明, 陆宴辉, 等. 六种杀虫剂对棉铃虫的毒力效果比较[J]. 应用昆虫学报, 2018, 55(1): 61-66.
ZHANG Dandan, YANG Xianming, LU Yanhui, et al. Comparison of the toxicity of six insecticides against the cotton bollworm Helicoverpa armigera[J]. Chinese Journal of Applied Entomology, 2018, 55(1): 61-66.
[3] 郭予元. 棉铃虫的研究 [M].北京: 中国农业出版社, 1998.
GUO Yuyuan. Studies on cotton bollworm[M]. Beijing: China Agriculture Press, 1998.
[4] 陆宴辉,姜玉英,刘杰, 等.种植业结构调整增加棉铃虫的灾变风险[J].应用昆虫学报,2018,55(1):19-24.
LU Yanhui, JIANG Yuying, LIU Jie, et al. Adjustment of cropping structure increases the risk of cotton bollworm outbreaks in China [J]. Chinese Journal of Applied Entomology, 2018, 55(1):19-24.
[5] Delorenzo M E, Serrano L, Chung K W, et al. Effects of the insecticide permethrin on three life stages of the grass shrimp [J]. Ecotoxicology and Environmental Safety, 2006, 64(2): 122-127.
[6] Norment B R, Chambeas H W. Temperature relationships in organophosphorus poisoning in boll weevils [J]. Journal of Economic Entomology, 1970, 63(2): 502-504.
[7] Chalfant, R. B. Cabbage Looper: Effect of Temperature on Toxicity of Insecticides in the Laboratory [J]. Journal of Economic Entomology, 1973, 66(2):339-341.
[8] Harwood A D, You J, Lydy M J . Temperature as a toxicity identification evaluation tool for pyrethroid insecticides: toxicokinetic confirmation.[J]. Environmental Toxicology & Chemistry, 2010, 28(5): 1051-1058.
[9]马云华. 几类杀虫剂对麦长管蚜和绿盲蝽的温度系数及温度对主要代谢酶的影响 [D]. 保定: 河北农业大学, 2012.
MA Yunhua. Effect of temperature on the toxicity of several insecticides to Sitobionavenae (Fabricius) and Apolyguslucorum (Meyer-Dür) and on the enzyme activity[D]. Baoding: Hebei Agricultural University, 2012.
[10]常秀辉,范晓溪,班兰凤, 等. 杀虫剂四氯虫酰胺对美国白蛾的室内活性及田间药效评价[J].农药,2018,57(10):762-763.
CHANG Xiuhui, FAN Xiaoxi, BAN Lanfeng, et al. Bioactivity and field efficacy of the novel compound silvchongxian′an against Hyphantriacunea[J]. Agrochemicals, 2018, 57(10):762-763.
[11] 窦亚楠,安静杰,高占林, 等. 双酰胺类杀虫剂对甜菜夜蛾毒力的温度效应[J].新疆农业科学,2020,57(4):658-664.
DOU Yanan, AN Jingjie, GAO Zhanlin, et al.Temperature effect on the toxicity of diamide insecticides to Spodopteraexigua Hübner [J]. Xinjiang Agricultural Sciences, 2020,57(4):658-664.
[12] Li Y F, Dou Y N, An J J, et al. Temperature-dependent variations in toxicity of diamide insecticides against three lepidopteran insects[J]. Ecotoxicology, 2020, 29(5):607-612.
[13]徐莉, 王建华, 梅宇, 等. 解毒酶和转运蛋白介导的害虫抗药性分子机制研究进展 [J]. 农药学学报, 2020, 22(1): 1-10.
XU Li, WANG Jianhua, MEI Yu, et al. Research progress on the molecular mechanisms of insecticidesresistance mediated by detoxification enzymes and transporters [J]. Chinese Journal of Pesticide Science, 2020, 22(1): 1-10.
[14]马云华,高占林,李耀发, 等.四种类型杀虫剂对麦长管蚜的温度效应及其与主要解毒酶的关系[J].应用昆虫学报,2011,48(6):1661-1668.
MA Yunhua, GAO Zhanlin, LI Yaofa, et al. Effect of temperature on the toxicity of several insecticides to the English grain aphid, Sitobionavenae[J]. Chinese Journal of Applied Entomology, 2011,48(6):1661-1668.
[15]刘佳,高占林,党志红, 等.不同温度效应杀虫剂诱导对绿盲蝽三种解毒酶活力的影响[J].应用昆虫学报,2015,52(3):609-615.
LIU Jia, GAO Zhanlin, DANG Zhihong, et al. The effect of temperature on the activity of three detoxification enzymes inApolyguslucorum[J]. Chinese Journal of Applied Entomology, 2015, 52(3):609-615.
[16] 冉春, 陈志永, 陈洋, 等. 桔全爪螨田间种群对双甲脒的敏感性及其两种酶的生化特性 [J]. 中国农学通报, 2007, 23(11): 284-288.
RAN Chun, CHEN Zhiyong, CHEN Yang, et al. Susceptibility to amitraz and biochemical characterizations of two enzymes from Panonychuscitri field populations [J]. Chinese Agricultural Science Bulletin, 2007, 23(11): 284-288.
[17] Clark A G, Dick G L, Smith J N. Kinetic studies on a glutathione S-transferase from the larvae of Costelytrazealandica[J]. Biochemical Journal, 1984, 217(1): 51-58.
[18] Kim Y J, Lee S W, Cho J R, et al. Multiple resistance and biochemical mechanisms of dicofol resistance in Tetranychusurticae(Acari: Tetranychidae) [J]. Journal of Asia-Pacific Entomology, 2007, 10(2): 165-170.
[19] 娄琳琳. 杀虫剂对斜纹夜蛾UDP-葡萄糖基转移酶活性的影响 [D]. 南京: 南京农业大学, 2014.
LOU Linlin. Effect of pesticides on UDP-glycosyl transferases activity in Spodopteralitura[D]. Nanjing: Nanjing Agricultural University, 2014.
[20] 王梦瑶. 朱砂叶螨UGT201D3基因参与阿维菌素抗药性的机制研究 [D]. 重庆: 西南大学, 2018.
WANG Mengyao. Mechanism analysis of UGT201D3 associated with abamectin resistance in Tetranychuscinnabarinus (Boisduval) [D]. Chongqing: Southwest University, 2018.
[21] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Analytical Biochemistry, 1976, 72: 248-254.
[22]张晓燕. 高温对几种菜田昆虫乙酰胆碱酯酶及解毒酶系活性影响的研究[D]. 福州: 福建农林大学,2010.
ZHANG Xiaoyan. Effects of high tempertaure on acetylcholinesterase and detexification enzymes in several insects in vegetable fields[D]. Fuzhou: Fujian Agricultural and Forestry University, 2010.
[23]王龙. 四氯虫酰胺对小菜蛾的亚致死效应研究 [D]. 晋中:山西农业大学, 2016.
WANG Long. Sublethal effects of SYP-9080 applied on Plutellaxylostella (Lepidoptera: Yponomeutidae) [D]. Jinzhong: Shanxi Agricultural University, 2016.
[24] Mao T T, Li F C, Fang Y L, et al. Effects of chlorantraniliprole exposure on detoxification enzyme activities and detoxification-related gene expression in the fat body of the silkworm, Bombyx mori [J]. Ecotoxicology & Environmental Safety, 2019, 176:58-63.
[25]Guo L, Su M M, Liang P, et al. Effects of high temperature on insecticide tolerance in whitefly Bemisia tabaci (Gennadius) Q biotype[J]. Pesticide Biochemistry and Physiology, 2018, 150: 97-104.
[26]刘佳. 杀虫剂对绿盲蝽毒力的温度效应及其解毒酶相关机制研究[D]. 保定:河北农业大学, 2015.
LIU Jia. Temperature effect on toxicity of insecticides to Apolyguslucorum (Meyer-Dür) and the related mechanism on detoxification enzymes[D]. Baoding: Hebei Agricultural University, 2015.
[27] An J J, Liu C, Dou Y N, et al. Analysis of differentially expressed transcripts inApolyguslucorum(Meyer-Dür) exposed to different temperature coefficient insecticides [J]. International Journal of Molecular Sciences, 2020,21(2): 658.

四氯虫酰胺对棉铃虫生物活性的温度效应及其解毒酶相关机制

Temperature Effect of Tetrachlorantraniliprole on the Biological Activity to Helicoverpa armigera Hübner and the Related Mechanism on Detoxification Enzymes

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	杨世英, 马玉娥, 贾斌鑫, 华震宇, 沈琦, 刘峰娟, 王成. 油菜素内酯对甜瓜农药残留的降解作用分析[J]. 新疆农业科学, 2023, 60(10): 2541-2550.
[2]	宋雯雯, 陈卓, 李鑫, 陈俐静, 舒展, 李紫仟, 郭雪萍, 叶倩文, 马雪连, 苏战强, 姚刚. 准噶尔双峰驼驼乳营养与功能成分与荷斯坦牛乳的差异比较分析[J]. 新疆农业科学, 2023, 60(1): 234-241.
[3]	麻正辉, 苏悦, 马雪, 羊河, 侯国辉, 陈柄润, 韩旭, 姚永生. 噻虫嗪种子包衣棉花对棉蚜和棉长管蚜解毒酶活性的影响[J]. 新疆农业科学, 2021, 58(9): 1685-1693.
[4]	李维政, 宁忠雄, 梁赫, 路伟. UPLC-MS/MS测定棉铃虫体内丁布的残留量[J]. 新疆农业科学, 2021, 58(10): 1846-1850.
[5]	唐睿, 余浪, 高永健, 王昊杨, 郑哲, 卡哈尔曼·胡吉, 杨涵, 张金平, 张峰, 杨普云, 郭荣. 新疆棉田硫丹防虫替代技术的筛选与评价[J]. 新疆农业科学, 2020, 57(6): 1071-1080.
[6]	马星宇, 嵇玉洁, 薛玉莹, 杜孟芳, 魏纪珍, 尹新明, 刘晓光, 安世恒. 棉铃虫鞣化激素基因克隆及分子特性分析[J]. 新疆农业科学, 2020, 57(4): 589-599.
[7]	嵇玉洁, 马星宇, 宋永辉, 杜孟芳, 魏纪珍, 尹新明, 刘晓光, 安世恒. 棉铃虫鞣化激素β亚基参与脂肪组织免疫的相关功能基因表达模式[J]. 新疆农业科学, 2020, 57(4): 600-607.
[8]	于健, 查萌, 郑梦君, 刘小侠. 白僵菌和绿僵菌对棉铃虫的室内防控效果评价[J]. 新疆农业科学, 2020, 57(4): 608-615.
[9]	窦亚楠, 安静杰, 高占林, 党志红, 潘文亮, 李耀发. 双酰胺类杀虫剂对甜菜夜蛾毒力的温度效应[J]. 新疆农业科学, 2020, 57(4): 658-664.
[10]	吴娜, 张玉栋, 蔡晓虎, 史亚辉, 韩睿, 王俊刚. 棉蚜取食被棉长管蚜危害棉花后其相关酶的活性[J]. 新疆农业科学, 2020, 57(11): 2056-2064.
[11]	芦屹, 王惠卿, 魏新政, 高永健, 代明, 李晶. 害虫远程实时监测系统监测棉铃虫成虫消长动态[J]. 新疆农业科学, 2019, 56(3): 521-527.
[12]	郭志刚,安静杰,高占林,党志红,潘文亮,袁文龙,李耀发. 苯甲酰基脲类杀虫剂对绿盲蝽生物活性的测定[J]. 新疆农业科学, 2019, 56(1): 61-66.
[13]	郭天凤;周晶;叶玉霞;王国平;蔡晓莉;刘铨义;曾庆涛;冯洋;赵富强. 棉蚜啶虫脒不同品系解毒酶活性测定和增效剂作用的研究[J]. , 2015, 52(7): 1334-1339.
[14]	马天文;王俊刚;何泽敏;江海澜;邓小霞;彭俊. 甲氨蝶呤和紫杉醇对棉铃虫繁育能力的影响[J]. , 2012, 49(3): 482-487.
[15]	何海;朱燕;刘宁;晁群芳;刘小宁. 棉铃虫中肠谷胱甘肽S-转移酶(GST)对新疆一枝蒿黄酮提取物和萜类提取物的响应[J]. , 2012, 49(12): 2254-2262.