1株新疆胡杨林昆虫病原线虫鉴定及生物学特性

doi:10.6048/j.issn.1001-4330.2020.03.018

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (3): 536-544.DOI: 10.6048/j.issn.1001-4330.2020.03.018

• 植物保护·土壤肥料·农业机械·畜牧兽医 • 上一篇下一篇

1株新疆胡杨林昆虫病原线虫鉴定及生物学特性

詹发强, 侯敏, 杨蓉, 王宁, 包慧芳, 侯新强, 崔卫东, 龙宣杞

新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室,乌鲁木齐 830091

收稿日期:2019-10-19 出版日期:2020-03-20 发布日期:2020-03-11
通信作者: 龙宣杞(1969-),女,四川人,研究员,硕士生导师,研究方向为农业微生物应用,(E-mail)longxq_xj@sina.com
作者简介:詹发强(1980-),男,副研究员,研究方向为农业微生物应用,(E-mail) zfq_xj@126.com
基金资助:
新疆维吾尔自治区自然科学基金(2019D01A62);新疆农业科学院青年科技骨干创新能力培养项目(xjnkq-2019016)

Research on Steinernema litorale from Populus euphratica Forest in Xinjiang by Identification and Biological Characteristics

ZHAN Faqiang, HOU Min, YANG Rong, WANG Ning, BAO Huifang, HOU Xinqiang, CUI Weidong, LONG Xuanqi

Xinjiang Key Laboratory of Special Environmental Microbiology / Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2019-10-19 Online:2020-03-20 Published:2020-03-11
Correspondence author: LONG Xuanqi(1969-), female, research direction: Application of agricultural microorganisms, (E-mail)609160628@qq.com
Supported by:
Supported by the Natural Science Foundation of Xinjiang Uygur Autonomous Region(2019D01A62); the Project of Renovation Capacity Building for the Young Sci-Tech Talents Sponsored by Xinjiang Academy of Agricultural Sciences(xjnkq-2019016)

摘要/Abstract

摘要： 【目的】对新疆天然胡杨林中昆虫病原线虫(EPNs)进行分离、鉴定并测试其杀虫活性,为胡杨林害虫生物防治提供新的选择。为新疆天然胡杨林中EPNs资源挖掘和基于EPNs的胡杨林害虫生物防治提供资源储备和奠定研究基础。【方法】利用经典方法,诱导及富集EPNs、分子生物学与形态学相结合鉴定,以及不同剂量杀虫测试分析其种属及生物学特性。【结果】在新疆天然胡杨林中存在EPNs,采集地检出率达25%。线虫18S rDNA系统发育树分析其在进化关系上与Steinernema litorale strain Pak.S.P.7 进化关系最近,获得基因登录号No: MK416208;形态学表明其与Steinernema litorale模式株大部分特征值基本吻合,主要区别在于第1代雄虫:体长略长(1 461.01 μm)、排泄孔至头部距离短(86.98 μm)、交合刺短(69.49 μm)、D%(60.45)与SW%158.21 值小于模式株;侵染期幼虫:体长(885.88 μm)和尾长(74.81 μm)短、E%(83.88)值大。共生细菌16S rDNA系统发育树分析与Xenorhabdus bovienii strain Zakharovo 的进化距离最近。杀虫分析表明,在160IJs/Gm (Galleria mellonella) 及其以上处理组36 h之内校正致死率达到100%;对黄粉虫60 h内40IJs/Tm (Tenebrio molitor) 及以上剂量校正致死率超过50%,160IJs/Tm及以上试验组校正致死率超过75%。【结论】在新疆天然胡杨林中发现有EPNs的分布,Steinernema litorale 在采集地是优势种,同时其具有较强的杀虫能力。

关键词: 昆虫病原线虫; 胡杨林; 海滨斯氏线虫; 分离鉴定; 杀虫活性

Abstract: 【Objective】 The purpose of this research was to isolate, identify the species of Entomopathogenic nematodes in natural Populus euphratica forests in Xinjiang and test its insecticidal activity in the hope of providing a new choice for biological control of the insects. 【Method】 The EPNs were induced by Galleria mellonella and collected with white-trap method, and then identified with molecular and morphology and finally biological characteristics were tested by different dosages of EPNs to Galleria mellonella (Gm) and Tenebrio molitor (Tm). 【Results】 The results showed that EPNs existed in natural Populus euphratica forests in Xinjiang, and the detection rate was up to 25%. The phylogenetic analysis of 18S rDNA showed that it had the closest evolutionary relationship with Steinernema litorale strain Pak.S.P.7, and the obtained Genebank accession No was: MK416208. Morphological analysis showed that most of the eigenvalues of Steinernema litorale - Bachu were almost the same with the Steinernema litorale type strain, but the main differences were that: compared to the type strain, the length (1461.01 μm) of first generation male was slightly longer, the distance from anterior end to excretory pore (86.98 μm) was shorter, the spicule length (69.49 μm) was shorter, values of D% (60.45) and SW% (158.21) were lower; Compared to the type strain, the length (885.88 μm) of infective juveniles and tail were shorter. The phylogenetic analysis of 16S rDNA of symbiotic bacteria was the closest to Xenorhabdus bovienii strain Zakharovos. The insecticidal analysis of infective juveniles showed that the mortality was 100% within 36 hours in 160 IJs/Gm and above treatment groups. Mortality was 50% in 40 IJs/Tm and 75% in 160 IJs/Tm and above treatment groups for Tenebrio molitor within 60 hours. 【Conclusion】 The research first discovered the distribution of EPNs (Steinerma litorale) in natural Populus euphratica forest in Xinjiang, and it has high insecticidal activity. This will provide a resource reserve and research foundation for the exploitation of EPNs resources in natural Populus euphratica forests and the biological control of pests in Populus euphratica forests based on EPNs in Xinjiang in the future.

Key words: Entomopathogenic nematodes; Populus euphratica; Steinerma litorale; isolate and identification; insecticidal activity

中图分类号:

S188

詹发强, 侯敏, 杨蓉, 王宁, 包慧芳, 侯新强, 崔卫东, 龙宣杞. 1株新疆胡杨林昆虫病原线虫鉴定及生物学特性[J]. 新疆农业科学, 2020, 57(3): 536-544.

ZHAN Faqiang, HOU Min, YANG Rong, WANG Ning, BAO Huifang, HOU Xinqiang, CUI Weidong, LONG Xuanqi. Research on Steinernema litorale from Populus euphratica Forest in Xinjiang by Identification and Biological Characteristics[J]. Xinjiang Agricultural Sciences, 2020, 57(3): 536-544.

参考文献

[1] Bednarek A, Gaugler R. Compatibility of soil amendments with entomopathogenic nematodes [J]. Journal of Nematology, 1997, 29(2): 220.
[2] Ffrench-Constant R, Waterfield N, Daborn P, et al. Photorhabdus: towards a functional genomic analysis of a symbiont and pathogen [J]. FEMS Microbiology Reviews, 2003, 26(5): 433-456.
[3] 于海滨, 马娟, 王容燕, 等. 昆虫病原线虫对甘薯蚁象的致病力测定[J]. 中国生物防治学报, 2012, 28(4): 514-520.
YU Haibin, MA Juan, WANG Rongyan, et al. Virulence of Entomopathogenic Nematodes against Sweetpotato Weevils [J]. Chinese Journal of Biological Control, 2012, 28(4): 514-520.
[4] Ma J, Chen S, Moens M, et al. Efficacy of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) against the chive gnat, Bradysia odoriphaga[J]. Journal of Pest Science, 2013, 86(3): 551-561.
[5] Szalanski A L, Palmer T W, Mckay T, et al. Infectivity of Steinernema spp.(Nematoda: Steinernematidae) to adult litter beetles, Alphitobius diaperinus (Coleoptera: Tenebrionidae) in the laboratory[J]. Biocontrol Science and Technology, 2004, 14(1): 81-85.
[6] Smart Jr G C. Entomopathogenic nematodes for the biological control of insects [J]. Journal of Nematology, 1995, 27(4S): 529.
[7] Bell N L, Jackson T A, Nelson T L. The potential of entomopathogenic nematodes as biocontrol agents for clover root weevil (Sitona lepidus)[J]. New Zealand Plant Protection, 2000, 53: 48-53.
[8] San-Blas E, Campos-Herrera R, Dolinski C, et al. Entomopathogenic nematology in Latin America: A brief history, current research and future prospects [J]. Journal of Invertebrate Pathology, 2019.
[9] Kepenekci I , Hazir S, Oksal E , et al. Application methods of, Steinernema feltiae, Xenorhabdus bovienii and Purpureocillium lilacinum to control root-knot nematodes in greenhouse tomato systems [J]. Crop Protection, 2018, 108:31-38.
[10] Garriga A, Morton A, García-López D, et al. Compatibility of entomopathogenic nematodes with natural enemies for horticultural pest control [J]. Biological Control, 2019: 104050.
[11] Devi G, Nath D. Entomopathogenic nematodes: A tool in biocontrol of insect pests of vegetables -A review [J].2017.
[12] Campos-Herrera R, Stuart R J, Pathak E, et al. Temporal patterns of entomopathogenic nematodes in Florida citrus orchards: Evidence of natural regulation by microorganisms and nematode competitors [J]. Soil Biology and Biochemistry, 2018.
[13] Shahina F, Tabassum K A , Habib M A . Potential of EPN in management of cotton bollworms in Pakistan [J]. Pakistan Journal of Nematology, 2014, 32:85-90.
[14] 孙瑞红, 武海斌, 宫庆涛, 等. 利用昆虫病原线虫防治几种果树害虫的关键技术[J]. 落叶果树, 2018 (5): 12.
SUN Hongrui, WU Haibing, GONG Qingtao, et al. Key Techniques for Controlling Several Fruits Pests Using Entomopathogenic Nematodes [J]. Deciduous Fruits, 2018 (5): 12.
[15] 吴文丹, 尹姣, 曹雅忠, 等. 我国昆虫病原线虫的研究与应用现状[J]. 中国生物防治学报, 2014, 30(6): 817-822.
WU Wendan, YIN Jiao, CAO Yazhong, et al. Entomopathogenic Nematodes and Their Application in China [J]. Chinese Journal of Biological Control, 2014, 30(6): 817-822.
[16] Yoshida M. Steinernema litorale n. sp.(Rhabditida: Steinernematidae), a new entomopathogenic nematode from Japan[J]. Nematology, 2004, 6(6): 819-838.
[17] Shahina F, Tabassum K A, Salma J, et al. Redescription of Steinernema litorale Yoshida, 2004 (Nematoda: Steinernematidae) from Pakistan on the basis of morphometriec, ITS1-5.8 S-ITS2; 28S (D2/D3) and mtDNA region[J]. International Journal of Nematology, 2012, 22(1/2): 81-93.
[18] MA J, CHEN S, ZOU Y, et al. Natural occurrence of entomopathogenic nematodes in North China[J]. Russian Journal of Nematology, 2010, 18(2).
[19] WANG H, LUAN J, Dong H, et al. Natural occurrence of entomopathogenic nematodes in Liaoning (Northeast China)[J]. Journal of Asia-Pacific Entomology, 2014, 17(3): 399-406.
[20] 刘瑞, 刘奇志. 海滨斯氏线虫生物学特性研究[J]. 中国生物防治学报, 2016, 32(1): 101-105.
LIU Rui, LIU Qizhi. Study on the Biological Characteristics of Steinernema litorale [J]. Chinese Journal of Biological Control, 2016, 32(1): 101-105.
[21] 张旭霞, 韩岚岚, 赵奎军, 等. 低温驯化对海滨斯氏线虫生化物质含量及其生存活力的影响[J]. 应用昆虫学报, 2018, 55(4):133-139.
ZHANG Xuxia, HAN Lanlan, ZHAO Kuijun, et al. Effects of cold acclimation on the biochemical content and survival of Steinernema litorale [J]. Chinese Journal of Applied Entomology, 2018, 55(4):133-139.
[22] 詹发强, 布卡·欧尔娜, 侯敏, 等. 新疆嗜菌异小杆线虫及其共生细菌的初步鉴定研究[J]. 新疆农业科学, 2015, 52(2):244-251.
ZHAN Faqiang, Buka Ouerna, HOU Min, et al. Preliminary Identification of Entomopathogenic Nematodes (Heterorhabditis bacteriophora strain Nileke) and Its Symbiotic Bacteria in Xinjiang [J]. Xinjiang Agricultural Sciences, 2015, 52(2):244-251.
[23] Nguyen K, Hunt D. Entomopathogenic Nematodes: Systematics, Phylogeny and Bacterial Symbionts [M]. Brill, 2007.
[24] 刘红兵, 刘奇志. 来自新疆的昆虫病原线虫的鉴定及生物学特性的研究[J]. 中国生物防治学报, 2016, 32(1): 106-111.
LIU Hongbing, LIU Qizhi. Identification and Biological Characteristics of Entomopathogenic Nematode Isolated from Xinjiang [J]. Chinese Journal of Biological Control, 2016, 32(1): 106-111.
[25] 吕洋, 韩岚岚, 赵奎军, 等. 哈尔滨2种斯氏线虫及其共生菌的分离鉴定和低温侵染力分析[J]. 中国生物防治学报, 2014, 30(4): 558-563.
LÜ Yang, HAN Lanlan, ZHAO Kuijun, et al. Isolation and Identification of Entomopathogenic Nematodes and Their Symbiotic Bacteria from Harbin (Northeast China) and Infectivity of Entomopathogenic Nematodes in Low Temperature [J]. Chinese Journal of Biological Control, 2014, 30(4): 558-563.

1株新疆胡杨林昆虫病原线虫鉴定及生物学特性

Research on Steinernema litorale from Populus euphratica Forest in Xinjiang by Identification and Biological Characteristics

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	徐李娟, 陈勇, 王则玉, 王博, 艾尼江·尔斯满, 郭瑞, 李克梅, 宋素琴. 新疆拜城县马铃薯疮痂病病原菌的分离鉴定及生物学特性分析[J]. 新疆农业科学, 2023, 60(9): 2258-2265.
[2]	陈澄, 侯新强, 詹发强, 杨蓉, 包慧芳, 王宁, 史应武, 龙宣杞. 三种昆虫病原线虫非生物胁迫耐受特性及其共生菌抑菌作用[J]. 新疆农业科学, 2023, 60(12): 3072-3079.
[3]	宋素琴, 高海峰, 吕卓, 唐琦勇, 顾美英, 张志东, 楚敏, 朱静, 王玮. 马铃薯疮痂病病原菌的分离鉴定及其生长特性分析[J]. 新疆农业科学, 2022, 59(8): 1999-2006.
[4]	高宇洁, 詹发强, 陈澄, 包慧芳, 杨蓉, 王宁, 侯新强, 侯敏, 史应武, 龙宣杞. 黑曲霉拮抗菌Xenorhabdus bovienii445筛选、鉴定及发酵优化[J]. 新疆农业科学, 2022, 59(5): 1203-1215.
[5]	高勇刚, 高海峰, 吕卓, 徐李娟, 古丽尼沙·沙依木, 唐琦勇, 顾美英, 张丽娟, 朱锦泉, 宋素琴. 新疆北部沿天山主要种植区马铃薯病害分析[J]. 新疆农业科学, 2022, 59(1): 170-178.
[6]	方涛, 张军高, 樊国全, 陈勇, 梁晶, 李进, 周小云, 王莉, 郑瑞明, 鄢蓉, 雷斌. 28种植物提取物对棉蚜的杀虫活性测定[J]. 新疆农业科学, 2021, 58(10): 1868-1875.
[7]	吕卓, 高勇刚, 唐琦勇, 楚敏, 顾美英, 朱静, 程思琦, 马晓雪, 宋素琴, 王静. 奇台窖藏马铃薯病害病原鉴定[J]. 新疆农业科学, 2020, 57(5): 902-909.
[8]	巴哈提古丽·马那提拜, 师璐, 李晓岩, 苏欢, 张雪妮, 房芳, 蒋刚强. 感官异常番茄酱和杏酱中腐败细菌的分离及分子生物学鉴定[J]. 新疆农业科学, 2019, 56(8): 1495-1501.
[9]	韩猛立, 张星星, 吴桐忠, 郭强强, 黄新, 钟发刚. 兔源巴氏杆菌分离与鉴定[J]. 新疆农业科学, 2018, 55(7): 1333-1342.
[10]	王新英, 史军辉, 刘茂秀. 塔里木河流域天然胡杨林营养积累特征及动态变化[J]. 新疆农业科学, 2018, 55(6): 1036-1045.
[11]	邱琳,王蕾,罗磊,郑江华. 基于时间序列塔里木河中游胡杨林春尺蠖危害的遥感监测[J]. 新疆农业科学, 2018, 55(3): 518-527.
[12]	代婧, 彭斌, 雷程红, 阿热阿依·海依拉提. 新疆部分地区牛源空肠弯曲菌分离鉴定及耐药性分析[J]. 新疆农业科学, 2017, 54(9): 1730-1736.
[13]	吐尔逊阿依·麦麦提依明;玛依古丽·库尔班;马丽艳木·阿木东;米尔班古丽·阿卜杜如苏力;努斯热提古丽·安外尔;艾克拜尔·木哈塔尔;祖丽皮亚·玉努斯;艾尔肯·热合曼. 新疆Kiyik废弃古河道天然胡杨林地可培养细菌的多样性研究[J]. , 2016, 53(5): 921-934.
[14]	布卡·欧尔娜;詹发强;龙宣杞;罗明;侯敏;杨蓉. 异小杆线虫NLK-1大量增殖技术研究[J]. , 2015, 52(9): 1693-1699.
[15]	宋素琴;楚敏;王玮;朱静;顾美英;唐琦勇. 一株高产多糖耐辐射菌的分离鉴定及其多糖的相关性质研究[J]. , 2014, 51(12): 2262-2268.