肉用绵羊超数排卵效果实例分析

doi:10.6048/j.issn.1001-4330.2017.08.022

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (8): 1550-1558.DOI: 10.6048/j.issn.1001-4330.2017.08.022

肉用绵羊超数排卵效果实例分析

郭延华, 万鹏程, 刘长彬

省部共建绵羊遗传改良与健康养殖国家重点实验室/新疆农垦科学院畜牧兽医研究所,新疆石河子 832000

收稿日期:2017-03-06 发布日期:2020-07-22
通信作者: 刘长彬(1977-),男,河南虞城人,副研究员,研究方向为牛羊胚胎工程,(E-mail)406271412@qq.com
作者简介:郭延华(1983-),男,新疆喀什人,助理研究员,硕士,研究方向为动物遗传育种与繁殖,(E-mail)guoyanhuatlr@sohu.com
基金资助:
兵团院士基金“绵羊高效MOET生产及受体处理程序优化研究”(2013BB023),兵团科技攻关与成果转化计划项目“新品种选育与种质资源创新(超细毛羊)”(2016AC027),国家自然科学基金项目“基于羔羊体外成熟卵母细胞转录组分析及核质成熟相关机制研究”(31160460)

A Retrospective Survey Analysis of Superovulation in Mutton Sheep

GUO Yan-hua, WAN Peng-cheng, LIU Chang-bin

State Key Laboratory of Sheep Genetic Improvement and Healthy Production/ Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi Xinjiang 832000, China

Received:2017-03-06 Published:2020-07-22
Correspondence author: Liu Chang-bin(1977-),male, native place: Yucheng, Henan. Associate Researcher, research field: Bovine and ovine embryo engineering.(E-mail)406271412@qq.com
Supported by:
Academician Fund of XPCC "Optimization of High Efficiency MOET Production and Receptor Processing in Sheep" (2013BB023); Project of Tackling Key Scientific Problems and Achievements Transformation "Breeding of New Varieties and Innovation of Germplasm Resources (Superfine Wool Sheep)" (2016AC027) and National Natural Foundation of China "Study on the Transcriptome Analysis and Cytoplasmic Maturation of Lamb Mature Oocytes in Vitro" (31160460)

摘要/Abstract

摘要： 【目的】超排胚胎移植技术是提高绵羊遗传潜力的一项重要应用技术,但影响供体超数排卵的因素很多,也需要大量数据支持。【方法】以影响供体超排的因素做回顾性调查分析,从激素来源、肉羊品种、日粮水平、年龄、超排间隔和超排剂量等6个方面观察对供体超排的影响。【结果】国产FSH超排效果与进口FSH差异不显著(P＞0.05),肉羊品种间无显著差异性(P＞0.05),年龄对供体超排反应影响显著(P＜0.05),营养对供体的超排反应显著(P＜0.05),供体以短间隔超排重复利用获得胚胎数量最多,增加超排剂量能显著改善超排反应(P＜0.05),但对重复利用供体差异不明显(P＞0.05)。【结论】对超排反应有影响的因素分为显著的和不显著的因素,显著的因素包括年龄、营养、超排间隔、超排剂量,而不显著的因素为品种。

关键词: 绵羊; 超排胚胎移植; 超排反应; 影响因素

Abstract: 【Objective】 Multiple ovulation and embryo transfer ( MOET ) is an important applied technology to improve the genetic potential of sheep. There are many factors that affect donor superovulation, and a large amount of data is needed to support it.【Method】A retrospective survey about the factors such as Follicle stimulating hormone resource, breed, daily ration, age, superovulation interval and dosage were analyzed and summarized between superovulation responses in our application for mutton sheep.【Result】The results showed that domestic FSH was equal to Canadian FSH in superovulation response (P＞0.05), different breeds of mutton sheep had no significant difference in superovulation response (P＞0.05); It was remarkable between youth and adult in the superovulation response (P＜0.05); It was the same to ration supply(P＜0.05). The donor received the largest number of embryos when repeatedly superovulated by short internal, the increased FSH dosage impacted superovulation response signivicantly in mutton sheep (P＜0.05), but continuous repeatedly superovulation had no obvious effect (P＞0.05).【Conclusion】In conclusion, the influencing factors could be divided into remarkable and indistinctive factors. Age, daily ration, superovulation interval and dosage can be relegated to the remarkable factors and breed can be classified to the indistinctive factors.

Key words: sheep; embryo transfer; superovulatory response; influencing factor

中图分类号:

S826.9⁺2

郭延华, 万鹏程, 刘长彬. 肉用绵羊超数排卵效果实例分析[J]. 新疆农业科学, 2017, 54(8): 1550-1558.

GUO Yan-hua, WAN Peng-cheng, LIU Chang-bin. A Retrospective Survey Analysis of Superovulation in Mutton Sheep[J]. Xinjiang Agricultural Sciences, 2017, 54(8): 1550-1558.

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肉用绵羊超数排卵效果实例分析

A Retrospective Survey Analysis of Superovulation in Mutton Sheep

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