甘蓝型油菜含油量性状的QTL定位

doi:10.6048/j.issn.1001-4330.2017.08.008

新疆农业科学 ›› 2017, Vol. 54 ›› Issue (8): 1437-1443.DOI: 10.6048/j.issn.1001-4330.2017.08.008

甘蓝型油菜含油量性状的QTL定位

王燕惠¹, 顾元国¹, 范李萍¹, 段雅洁¹, 高文伟¹, 曲延英¹, 陈跃华²

1.新疆农业大学农学院/新疆农业大学农业生物技术试验室,乌鲁木齐 830052;
2.新疆农业科学院经济作物研究所,乌鲁木齐 830091

收稿日期:2017-06-05 发布日期:2020-07-22
通信作者: 高文伟(1973-),男,陕西乾县人,教授,研究方向为作物遗传育种,(E-mail)280594606@qq.com;陈跃华(1958-),男,新疆昌吉人,研究员,研究方向为油菜遗传育种与栽培,(E-mail)chenyuehua158576 @ sina.com
作者简介:王燕惠(1989-),女,福建安溪人,硕士研究生,研究方向为作物遗传育种,(E-mail)280594606@qq.com
基金资助:
自治区高技术研究发展计划“新疆甘蓝型春油菜分子标记辅助杂种培育技术应用”(201311109)

Mapping QTLs for Oil Content Traits in Brassica napus L.

WANG Yan-hui¹, GU Yuan-guo², FAN Li-ping¹, DUAN Ya-jie¹, GAO Wen-wei¹, QU Yan-ying¹, CHEN Yue-hua²

1. College of Agronomy, Xinjiang Agricultural University/Key Laboratory of Agriculture Biological Technology, Xinjiang Agricultural University, Urumqi 830052, China;
2. Research Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2017-06-05 Published:2020-07-22
Correspondence author: GAO Wen-wei (1973-) male, native place:Shanxi, Professor, research field: Crop genetics and breeding. (E-mail)280594606@qq.com; CHEN Yue-hua (1958-), male, Changji, Xinjiang ,Researcher,research field: Genetics, breeding and cultivation of rape,(E-mail)chenyuehua158576@sina.com
Supported by:
Autonomous research and development plan for high technology "Application of molecular marker assisted hybrid breeding technique for Brassica napus in Xinjiang" (201311109)

摘要/Abstract

摘要： 【目的】开发与含油量相关的分子标记,发掘与该性状相关的基因。【方法】以Springfield-B和ymnm-8作为亲本构建F₂群体,利用SRAP分子标记构建甘蓝型油菜遗传连锁图谱。【结果】该遗传图谱含有52个标记位点,图谱全长1 039.6 cM,含有15个连锁群,标记间的平均遗传距离为19.9 cM。分析与含油量相关的QTL位点,获得了2个与含油量相关QTL位点。其中qOC1位于连锁群LG1:EM9ME6-EM17ME24标记区,qOC15位于连锁群LG1: EM3ME5-EM4ME22a标记区间,其表型变异解释率分别为11.23%和4.12%,加性效应值分别为0.48和3.56。【结论】获得了2个与含油量相关QTL位点,为研究甘蓝型油菜含油量奠定了一定的基础。

关键词: 甘蓝型油菜; 含油量; 遗传连锁图谱; QTL定位

Abstract: 【Objective】 To locate the molecular markers related to the oil and explore the genes related to the trait. 【Method】In this study, F₂ population was constructed with Springfield-B and ymnm-8 as parents, and SRAP molecular markers were used to construct the genetic linkage map of Brassica napus L. 【Result】The results showed that the genetic map contained 52 marker sites with a total length of 1,039.6 cM and 15 linkage groups. The average genetic distance between the markers was 19.9 cM. The QTLs associated with oil content were analyzed and two QTLs related to oil content were obtained. QOC15 was located in the linkage group LG1: EM3ME5-EM4ME22a, and the explanatory rates were 11.23% and 4.12%, respectively, and the additive effect values were 0.48 and 3.56, respectively. The qOC1 was located in the linkage group LG1: EM9ME6-EM17ME24. 【Conclusion】Two QTL loci related to oil content were obtained, which laid a foundation for the study of oil content in Brassica napus L.

Key words: Brassica napus L.; oil content; genetic linkage map; QTL mapping

中图分类号:

S565.4

王燕惠, 顾元国, 范李萍, 段雅洁, 高文伟, 曲延英, 陈跃华. 甘蓝型油菜含油量性状的QTL定位[J]. 新疆农业科学, 2017, 54(8): 1437-1443.

WANG Yan-hui, GU Yuan-guo, FAN Li-ping, DUAN Ya-jie, GAO Wen-wei, QU Yan-ying, CHEN Yue-hua. Mapping QTLs for Oil Content Traits in Brassica napus L.[J]. Xinjiang Agricultural Sciences, 2017, 54(8): 1437-1443.

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甘蓝型油菜含油量性状的QTL定位

Mapping QTLs for Oil Content Traits in Brassica napus L.

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