CEffects of Supplemented with Guanidinoacetic Acid on the Plasma Guanidinoacetic Acid and Creatine Concentrations of Yili Horses During Exercise Training

doi:10.6048/j.issn.1001-4330.2022.09.022

Abstract

Abstract:

【Objective】 The purpose of this experiment was to investigate the effect of guanidine acetic acid （GAA） supplementation on the concentration of guanidinacetic acid and creatine in plasma of Yili horse. 【Method】 Twenty Yili horses aged 2 to 3 years old， similar in weight （373.2±30.53） kg， and rigorously trained for speed races， were randomly divided into 4 groups and with 5 horses （3♀+2♂） in each group， and the training intensity were consistent. Each horse was fed 4 kg of concentrate supplements per day， and freely collected dry herbage. On this basis， each horse in each group was fed with 0 （CON group）， 25 （25 GAA group）， 50 （50 GAA group） and 75 mg/kg BW （75 GAA group） guanidine acetic acid respectively. 【Result】 The results showed as follows：（1） Supplementation of guanidine acetic acid significantly increased the plasma Cr/GAA， of horses in 50 GAA group from 1 to 10 days and 75 GAA group for 7 days and 10 days at 2 hours after feeding （P<0.01）， and significantly increased the plasma creatinine concentration at 2 hours after feeding for 7 days and 10 days in 50 GAA group （P<0.05）. （2） Supplementation of guanidine acetic acid significantly increased plasma Cr/GAA in all experimental groups （P<0.01）， and plasma creatine concentration and 24 h urine creatine metabolite excretion in 50 and 75 GAA groups were significantly increased （P<0.01）. 【Conclusion】 It can be concluded that under the conditions of this experiment， guanidine acetic acid supplementation increased the concentration of plasma creatine metabolites and promoted the rapid absorption of guanidine acetic acid in Yili horse， and the suitable level of guanidine acetic acid supplementation was 50 mg/kg BW.

Key words: Yili horses; training; guanidinoacetic acid; creatine; metabolism

摘要：

【目的】 研究补喂胍基乙酸（GAA）对伊犁马血浆胍基乙酸、肌酸浓度的影响。【方法】 选取20匹年龄为2~3岁、体重[（373.2±30.53）kg]相近且经过严格训练的速度赛用伊犁马，随机分为4组，每组5匹（3♀+2♂），每日等强度训练。每匹马每天饲喂精料补充料4 kg，自由采食青干草，各组每天每匹马分别补喂0（CON组）、25（25 GAA组）、50（50 GAA组）和75 mg/kg BW（75 GAA组）的胍基乙酸。【结果】 （1）补喂胍基乙酸极显著提高了50 GAA组马匹第1~10 d以及75 GAA 组马匹7 d、10 d的饲喂后2 h血浆Cr/GAA（P<0.01），显著提高了50 GAA组马匹第7 d、10 d的饲喂后2 h血浆肌酐浓度（P<0.05）。（2）补喂胍基乙酸极显著提高了各试验组血浆Cr/GAA（P<0.01），极显著提高了50、75 GAA组马匹血浆肌酸浓度及24 h尿液肌酸代谢物排出量（P<0.01）。【结论】 补喂胍基乙酸提高了伊犁马血浆肌酸代谢物浓度，促进了伊犁马机体对胍基乙酸的快速吸收，以供机体利用，适宜添加水平为50 mg/kg BW。

关键词: 伊犁马, 训练, 胍基乙酸, 肌酸, 代谢

CLC Number:

S821

LIU Lv, LI Xiaobin, MA Yan, YANG Hao, ZHANG Wenjie, LU Hao, WEI Xinlan, GAO Junjie, OUYANG Wen, YANG Kailun. CEffects of Supplemented with Guanidinoacetic Acid on the Plasma Guanidinoacetic Acid and Creatine Concentrations of Yili Horses During Exercise Training[J]. Xinjiang Agricultural Sciences, 2022, 59(9): 2267-2275.

刘律, 李晓斌, 马艳, 杨昊, 张文杰, 鲁豪, 卫鑫岚, 高俊杰, 欧阳文, 杨开伦. 补喂胍基乙酸对运动训练伊犁马血浆胍基乙酸、肌酸浓度的影响[J]. 新疆农业科学, 2022, 59(9): 2267-2275.

Figures/Tables 8

References 23

[1]	Reardon T F, Ruell P A, Fiatarone S M A, et al. Creatine supplementation does not enhance submaximal aerobic training adaptations in healthy young men and women[J]. European Journal of Applied Physiology, 2006, 98(3):234-241. PMID
[2]	Maccormick V M, Hill L M, Macneil L, et al. Elevation of creatine in red blood cells in vegetarians and nonvegetarians after creatine supplementation[J]. Canadian Journal of Applied Physiology, 2004, 29(6):704-713. PMID
[3]	SEWELL D A, HARRIS R C. Effects of creatine supplementation in the thoroughbred horse[J]. Equine Veterinary Journal, 1995, 27(18):239-242. DOI URL
[4]	李晓斌, 马军, 聂彪彪, 等. 补喂肌酸对速步马运动性能、糖代谢及抗氧化能力的影响[J]. 动物营养学报, 2016, 28(5):1506-1514.
	LI Xiaobin, MA Jun, NIE Biaobiao, et al. Effects of creatine supplementation on exercise performance, glucose metabolism and antioxidation of speedhorses[J]. Chinese Journal of Animal Nutrition, 2016, 28(5):1506-1514. (in Chinese)
[5]	张德福, 田耀耀, 马佳, 等. 胍基乙酸对AA肉仔鸡生长性能和经济效益的影响[J]. 饲料研究, 2016,(17):32-35.
	ZHANG Defu, TIAN Yaoyao, MA Jia, et al. Effects of guanidine acetic acid on growth performance and economic benefit of AA broilers[J]. Feed Research, 2016,(17):32-35.
[6]	EDISON E E, BROSNAN M E, MEYER C, et al. Creatine synthesis: production of guanidinoacetate by the rat and human kidney in vivo[J]. Am J Physiol Renal Physiol, 2007, 293(6):1799-1804.
[7]	WEBER C J. Studies on the metabolism of guanidoacetic acid[J]. Hope-Seylers Z Physiol Chein, 1936,(114):cvii.
[8]	OSTOJIC S M. Guanidinoacetic acid as a performance-enhancing agent[J]. Amino Acids, 2016, 48(8):1867-1875. DOI PMID
[9]	OSTOJIC S M, NIESS B, STOJANOVIC M D, et al. Serum creatine, creatinine and total homocysteine concentration-time profiles after a single oral dose of guanidinoacetic acid in humans[J]. Journal of Functional Foods, 2014,(6):598-605.
[10]	LU Y, ZOU T, WANG Z, et al. Dietary guanidinoacetic acid improves the growth performance and skeletal muscle development of finishing pigs through changing myogenic gene expression and myofibre characteristics[J]. Journal of Animal Physiology and Animal Nutrition, 2020, 104(6):1875-1883. DOI URL
[11]	Setoue M, Ohuchi S, Morita T, et al. Hyperhomocysteinemia induced by guanidinoacetic acid is effectively suppressed by choline and betaine in rats[J]. Bioscience, Biotechnology, and Biochemistry, 2008, 72(7):1696-1703.
[12]	Wada T, Wada T, Shimbo H, et al. A simple screening method using ion chromatography for the diagnosis of cerebral creatine deficiency syndromes[J]. Amino Acids, 2012, 43(2):993-997. DOI PMID
[13]	WYSS M, KADDURAH-DAOUK R. Creatine and creatinine metabolism[J]. Physiological Reviews, 2000, 3(80):1107-1213.
[14]	王连生, 张圆圆, 单安山. 胍基乙酸的体内代谢及在动物生产中的应用[J]. 中国畜牧兽医, 2010, 37(6):13-16.
	Wang Liansheng, Zhang Yuanyuan, Shan Anshan. Metabolism of Guanidine Acetic Acid and Application in Animal Production[J]. Chinese Animal Husbandry and Veterinary Medicine, 2010, 37(6):13-16.
[15]	王誉杰, 张进威, 王讯, 等. 胍基乙酸及代谢产物肌酸的研究进展[J]. 畜牧兽医学报, 2018, 49(8):1577-1584.
	WANG Yujie, ZHANG Jinwei, WANG Xun, et al. Advances in studies on guanidine acetic acid and its metabolite creatine[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2018, 49(8):1577-1584.
[16]	晁雅琳, 刘博, 寇启芳, 等. 胍基乙酸对舍饲滩羊生长性能、屠宰性能、脂肪沉积及肌肉营养成分的影响[J]. 动物营养学报, 2019, 31(1):388-394.
	YAO Yalin, LIU Bo, KOU Qifang, et al. Effects of guanidine acetic acid on growth performance, slaughtering performance, fat deposition and muscle nutritional composition of house-fed tan sheep[J]. Chinese Journal of Animal Nutrition, 2019, 31(1):388-394.
[17]	Kodambashi Emami N, Golian A, Rhoads D D, et al. Interactive effects of temperature and dietary supplementation of arginine or guanidinoacetic acid on nutritional and physiological responses in male broiler chickens[J]. British Poultry Science, 2017, 58(1):87-94. DOI PMID
[18]	Majdeddin M, Braun U, Lemme A, et al. Guanidinoacetic acid supplementation improves feed conversion in broilers subjected to heat stress associated with muscle creatine loading and arginine sparing[J]. Poult Sci, 2020, 99(9):4442-4453. DOI URL
[19]	HE D T, GAI X R, YANG L B, et al. Effects of guanidinoacetic acid on growth performance, creatine and energy metabolism, and carcass characteristics in growing-finishing pigs[J]. Journal of Animal Science, 2018, 96(8):3264-3273. DOI PMID
[20]	Persky A M, Brazeau G A, Gunther H. Pharmacokinetics of the dietary supplement creatine[J]. Clinical Pharmacokinetics, 2003, 6(42):557-574.
[21]	Soboll S, Conrad A, Eistert A, et al. Uptake of creatine phosphate into heart mitochondria: a leak in the creatine shuttle[J]. Biochim Biophys Acta, 1997, 1320(1):27-33. PMID
[22]	LI S Y, WANG C, WU Z Z, et al. Effects of guanidinoacetic acid supplementation on growth performance, nutrient digestion, rumen fermentation and blood metabolites in angus bulls[J]. Animal, 2020, 14(12):2535-2542. DOI URL
[23]	TachikawA M, Ikeda S, FujinawA J, et al. γ-aminobutyric acid transporter 2 mediates the hepatic uptake of guanidinoacetate, the creatine biosynthetic precursor, in rats[J]. PLoS One, 2012, 7(2):1-9.

项目 Items	精料补充料 Concentrate supplement	青干草 Green hay
干物质 DM	92.06	94.11
有机物 OM	91.11	93.55
粗脂肪 EE	2.85	0.76
粗蛋白质 CP	18.29	12.94
中性洗涤纤维 NDF	25.82	59.33
酸性洗涤纤维 ADF	7.92	29.73
钙 Ca	0.77	0.93
磷 P	1.20	0.12

项目 Items	精料补充料 Concentrate supplement	青干草 Green hay
干物质 DM	92.06	94.11
有机物 OM	91.11	93.55
粗脂肪 EE	2.85	0.76
粗蛋白质 CP	18.29	12.94
中性洗涤纤维 NDF	25.82	59.33
酸性洗涤纤维 ADF	7.92	29.73
钙 Ca	0.77	0.93
磷 P	1.20	0.12

试验项目（d） Testing days	组别 Groups				P值 P-value
试验项目（d） Testing days	CON	25 GAA	50 GAA	75 GAA	总体	线性	二次
1	30.87±0.60^B	32.15±1.58^B	35.42±2.19^A	34.93±0.65^A	<0.001	<0.001	0.184
3	39.83±0.71^bB	37.21±0.64^cBC	46.56±0.78^aA	35.54±3.41^cC	<0.001	0.346	<0.001
5	43.81±1.72^cC	49.64±0.84^bB	56.65±1.82^aA	41.25±1.95^dC	<0.001	0.840	<0.001
7	37.26±0.81^D	47.60±0.38^B	55.22±0.78^A	41.58±1.45^C	<0.001	<0.001	<0.001
10	35.03±0.29^dC	42.88±2.03^bB	52.84±2.76^aA	38.04±1.76^cC	<0.001	<0.001	<0.001

试验项目（d） Testing days	组别 Groups				P值 P-value
试验项目（d） Testing days	CON	25 GAA	50 GAA	75 GAA	总体	线性	二次
1	30.87±0.60^B	32.15±1.58^B	35.42±2.19^A	34.93±0.65^A	<0.001	<0.001	0.184
3	39.83±0.71^bB	37.21±0.64^cBC	46.56±0.78^aA	35.54±3.41^cC	<0.001	0.346	<0.001
5	43.81±1.72^cC	49.64±0.84^bB	56.65±1.82^aA	41.25±1.95^dC	<0.001	0.840	<0.001
7	37.26±0.81^D	47.60±0.38^B	55.22±0.78^A	41.58±1.45^C	<0.001	<0.001	<0.001
10	35.03±0.29^dC	42.88±2.03^bB	52.84±2.76^aA	38.04±1.76^cC	<0.001	<0.001	<0.001

试验项目（d） Testing days	组别 Groups				P值 P-value
试验项目（d） Testing days	CON	25 GAA	50 GAA	75 GAA	总体	线性	二次
1	89.21±7.13	93.92±7.14	94.42±6.58	90.51±8.61	0.628	0.739	0.905
3	84.99±5.58	84.48±3.44	89.87±7.64	82.44±2.21	0.176	0.828	0.153
5	80.96±4.27	85.22±5.92	86.38±4.26	83.61±3.95	0.319	0.299	0.375
7	84.43±7.62^b	89.37±4.89^ab	93.65±6.25^a	86.38±4.64^ab	0.021	0.537	0.261
10	88.42±8.49^b	96.09±3.96^ab	100.81±7.98^a	91.72±6.22^ab	0.040	0.329	0.212